Table 1. Apparent kinetic parameters for hydrolytic editing of mischarged A. aeolicus [3H]Ile‐tRNALeuGAG
Proteinskcatapp (s−1)Kmapp (μM)kcatapp/Kmapp (μM−1 s−1)kcatapp/Kmapp (relative)Loss of efficiency (‐fold)
LeuRS
 αβ‐LeuRS1.30±0.121.70±0.250.7611
 αβ‐LeuRS‐Δ201.20±0.101.50±0.220.801.050.95
CP1
Aa‐CP15.0±0.7 × 10−221±2.82.38 × 10−33.0 × 10−3330
Aa‐CP1‐Δ201.0±0.2 × 10−230±3.20.33 × 10−34.3 × 10−42303
  • Catalytic efficiency values from five independent determinations were within ±10%. kcatapp and Kmapp are apparent kcat and Km, respectively.