Table 1. RHD proteins induced by TNFα stimulation in NF‐κB knockout cells
MEFs genotypeκB‐binding proteinsκB‐binding complexes in EMSA
p50p52p65cRel
wt++p50:p50ap50:p65p65:p65a
nfκb1−/−++p52:p65p65:p65a
nfκb1−/−nfκb2−/−+p65:p65
nfκb2−/−++p50:p50ap50:p65p65:p65a
rela−/−++p50:p50p50:cRelcRel:cRel
rela−/−crel−/−+p50:p50
crel−/−++p50:p50ap50:p65p65:p65a
nfκb1−/−rela−/−
  • Fibroblast cell lines derived from embryos of indicated genotypes (column 1) exhibit TNFα‐inducible κB‐binding activity that consists of indicated RHD proteins (column 2). These proteins form κB‐binding complexes indicated in column 3. All data are based on EMSA with κB‐site‐containing probes and antibodies directed against specific RHD proteins.

  • a Complexes detected with only a subset of κB‐site‐containing probes.