Table 1. Motility characteristics of mglA, mglB, and frz mutants
StrainGenotypeaReversal period (min)Velocity (μm min–1)
(a)
DK1622mglBA+13.93.2±0.3
DK6204ΔmglBANANA
SA3302ΔmglBA/mglBA+14.23.5±0.5
SA3303ΔmglBA/mglBAG21V4.62.6±0.2
SA3304ΔmglBA/mglBAT26/27NNANA
SA3306mglBA+/mglBA+14.03.6±0.2
SA3307mglBA+/mglBAG21V4.73.0±0.3
SA3308mglBA+/mglBAT26/27N483.4±0.7
SA3334mglBAG21V4.63.1±0.5
SA3335mglBAT26/27NNANA
(b)
DK8505frzlof, mglBA+>1002.2±0.4
SA3325frzlof, ΔmglBANANA
SA3318frzlof, ΔmglBA/mglBAG21V5.72.3±0.3
SA3321frzlof, ΔmglBA/mglBAT26/27NNANA
DK8506frzgof, mglBA+1.52.4±0.5
SA3324frzgof, ΔmglBANANA
SA3319frzgof, ΔmglBA/mglBAG21V5.01.6±0.2
SA3320frzgof, ΔmglBA/mglBAT26/27NNANA
(c)
DK3685mglA9NANA
SA2096mglA9/mglA+13.83.0±0.4
SA3359mglA9/yfp–mglA+12.92.8±0.2
SA3360mglA9/yfp–mglAG21V4.72.4±0.3
SA3361mglA9/yfp–mglAT26/27NNANA
(d)
SA3387ΔmglB6.53.0±0.4
SA3388ΔmglB/mglB–yfp12.42.8±0.2
SA3385ΔmglBA/yfp–mglA+6.82.4±0.3
SA3386ΔmglBA/mglB–yfpNANA
  • NA, not applicable.

  • a frzlof and frzgof alleles are frzCD∷Tn5lacΩ536 and frzCD∷Tn5Ω224, respectively.