Table 1. Kinetic and thermodynamic characterization of the binding of 8F9 Fab and M2J5 Fab to AD‐2S1
Biacore analysisITC analysis
ka (1 M−1 s−1)kd (1 s−1)KD (nM)KD (nM)ΔG° (kcal mol−1)ΔH° (kcal mol−1)ΔS° (cal mol−1 K−1)nT (K)
8F92.5 × 1058.5 × 10−334285
43−9.2−11.4−6.21.2287
3.5 × 1050.0412094−9.3−14.3−161.2297
240−9.3−16−21.91.2307
M2J54600−7−6.70.80.93287
19 000−6.4−11.2−16.10.95297