Table 1. Kinetic analysis of Dicer‐2 processing in the presence and absence of 25 mM phosphate. Data are mean ± standard deviation for three independent trials
SubstrateEnzymeKM (nM)Change in KMkcat (min)Change in kcatkcat/KM (nM/min)Change in kcat/KM
pre‐let‐7Dicer‐21500 ± 5001.02.0 ± 0.51.00.0014 ± 0.00011.0
Dicer‐2 + Phosphate3200 ± 10002.10.6 ± 0.20.310.00020 ± 0.000030.14
30‐bp dsRNADicer‐21100 ± 3001.02.7 ± 0.31.00.0026 ± 0.00031.0
Dicer‐2 + Phosphate2700 ± 6002.52.0 ± 0.20.730.00077 ± 0.000120.29
104‐bp dsRNADicer‐242 ± 101.00.086 ± 0.0081.00.0021 ± 0.00031.0
Dicer‐2 + Phosphate41 ± 50.980.090 ± 0.0081.00.0022 ± 0.00011.0