Table 1. Thermodynamic parameters of the interaction of Syntaxin1a and Munc18‐1 measured by ITC
Interaction ofKd (nM)ΔH° (kcal/mol)n
Syx1a1–262/M18wta1.4±0.3−34.6±0.21.03
Syx1a1–262/M18L130K28.2±3.7−22.7±0.30.91
Syx1a1–262/M18F115E6.6±1.4−25.8±0.20.91
Syx1a1–262/M18E59K48.1±5.6−14.7±0.20.98
Syx1a25–262/M18wta8.1±1.0−25.1±0.21.01
Syx1a25–262/M18L130K30.4±3.6−22.1±0.20.95
Syx1a25–262/M18F115E19.2±6.4−25.1±0.60.98
SNARE complex with Syx1a1–262/M18wta719.4±118.0−4.8±0.40.84
SNARE complex with Syx1a1–262/M18L130K
SNARE complex with Syx1a1–262/M18F115E
SNARE complex with Syx1a1–262/M18E59K
SNARE complex with Syx1a25–262/M18wt
  • The experimental isothermal titration calorimetry (ITC) data for the interaction of Syntaxin1a and Munc18‐1 variants are shown in Supplementary Figure 1. The errors reported in Table I are the numbers obtained from fitting the data of a single ITC run.

  • From Burkhardt et al, 2008.