Table 2. Cryo‐EM data collection, refinement and validation statistics
TssJLM core complex (EMD‐0264) (PDB 6HS7)TssJLM complex (EMD‐0265)TssJLM C1 complex (EMD‐0266)TssJLM base complex (EMD‐0267)
Data collection and processing
 Magnification120,000×120,000×120,000×120,000×
 Voltage (kV)200200200200
 Electron exposure (e–/Å2)120120120120
 Defocus range (μm)0.4–50.4–50.4–50.4–5
 Pixel size (Å)1.241.241.241.24
 Symmetry imposedC5C5C1C5
 Initial particle images (no.)167,825167,825167,825167,825
 Final particle images (no.)36,82836,82836,82836,828
 Map resolution (Å)4.64.97.917
 FSC threshold0.1430.1430.1430.143
 Map resolution range (Å)3.9 and 18 Å3.8–33 Å
Tomography
 Number of grid points
 Voxel size
 Projections
Refinement
 Initial model used (PDB code)4Y7O
 Model resolution (Å)
 FSC threshold4.6 Å
 Model resolution range (Å)
 Map sharpening B factor (Å2)
 Model composition
 Non‐hydrogen atoms52,890
 Protein residues6,905
 LigandsN/A
 B factors (Å2)
 Protein
 LigandN/A
 R.m.s. deviations
 Bond lengths (Å)0.006
 Bond angles (°)0.989
 Validation
 MolProbity score1.92
 Clashscore6.39
 Poor rotamers (%)0.04
 Ramachandran plot
 Favoured (%)89.46
 Allowed (%)10.54
 Disallowed (%)0