Table 3. Cryo‐ET data collection, refinement and validation statistics
STA_AvgVol_15000 (EMD‐4561)STA_AvgVol_23500 (EMD‐4562)
Data collection and processing
 Magnification42,00042,000
 Voltage (kV)300300
 Electron exposure (e–/Å2)9090
 Defocus range (μm)0 to −80 to −8
 Pixel size (Å)6.898 (binned once), or 3.45 unbinned6.898 (binned once), or 3.45 unbinned
 Symmetry imposedC5C5
 Initial particle images (no.)25,27628,463
 Final particle images (no.)15,00023,500
 Map resolution (Å)20
 FSC threshold0.143
 Map resolution range (Å)NA
Tomography
 Number of grid points928 × 928 × 400928 × 928 × 400
 Voxel size13.8 × 13.8 × 13.813.8 × 13.8 × 13.8
 Projections6161