Table 1. Chromosome copy number quantification from the karyotypes of late passage Ch9, 12, 10, and 14 MEFs
ChCh9 control copy numberCh9 ICL copy numberCh12 control copy numberCh12 ICL copy numberCh10 control copy numberCh10 ICL copy numberCh14 control copy numberCh14 ICL copy number
4Non‐44Non‐44Non‐44Non‐44Non‐44Non‐44Non‐44Non‐4
1713218128713020020417321
2191200191191182182183168
3173182191173173812174159
417b3b2b18b1911552b18b12b8b1741014
5191191191173191164138159
6164200182164191173138186
7020020119119020020318024
8155182191182173713183168
913a7a0a20a164164173155165159
1018218217319116a4a0a20a201186
11173191182155173128219123
1214610108a12a0a20a1737131110618
13173164191200155911516123
1416418216419116b4b5b15b17a4a0a24a
15182200191191191173174177
16164200182191155713201213
1718219120020014661421b0b16b8b
18164128173182182137183168
19173155191164173173183195
X20018218213715b5b4b16b10111311
Y146155
  • Repeat experiments were performed on ICL lines 9, 12, and 14, and no significant differences between ICL and control were observed in individual chromosomes in both replicates other than the targeted chromosome (data not shown). Since this Ch9 line was derived from a male embryo, the statistical tests for chromosomes X and Y were calculated for a copy number of 2 vs. non‐2.

  • a Statistically significant loss of targeted chromosome loss in the respective ICL line, in relation to the corresponding control (P < 0.05, n = 20 metaphases for Ch9, 12, 10; n = 21 for Ch14 control and n = 24 for Ch14 ICL). Fisher exact test was used to determine statistical significance and a false discovery correction was applied to the P‐values.

  • b Other significant chromosome number variations were not consistently observed in the same chromosome across all four lines (P < 0.05, n = 20 metaphases for Ch9, 10; n = 21 for Ch14 control and n = 24 for Ch14 ICL). Fisher exact test was used to determine statistical significance and a false discovery correction was applied to the P‐values.