Table 1. Crystallization, X‐ray data collection and refinement statistics for structures of wild‐type elF5B(517–858)
elF5B(517–858)·GTP·Na+elF5B(517–858)·GTPγS·Na+elF5B(517–858)·GTPγS·K+
Crystallization
Condition100 mM HEPES (pH 7.25), 12% PEG 4000, 100 mM NaOAc100 mM HEPES (pH 7), 13% PEG 4000, 125 mM NaOAc11% PEG 8000, 6% glycerol, 50 mM KCI
Temperature (°C)202020
Data collection
Space groupP21P21P41212
Unit cella = 55.6 Åa = 55.6 Åa = 116.1 Å
b = 116.5 Åb = 115.9 Åb = 116.1 Å
c = 66.2 Åc = 66.1 Åc = 120.3 Å
α = 90°α = 90°α = 90°
β = 101.1°β = 101 4°β = 90°
γ = 90°γ = 90°γ = 90°
Molecules/asym. unit222
Resolution (Å)1.55 (1.64–1.55)1.53 (1.63–1.53)2.28 (2.42–2.28)
Observed reflections405,616 (64,352)510,862 (86,738)307,464 (49,685)
Unique reflections118,431 (18,849)121,606 (20,942)38,004 (6,021)
Completeness (%)98.8 (97.7)98.5 (98.4)99.9 (99.7)
<I>/σ15.5 (2.3)18.8 (2.8)19.3 (3.8)
Rsym (%)4.6 (50.5)3.7 (49.6)6.3 (54.2)
CC (1/2) (%)99.9 (75.1)99.9 (82.2)99.9 (99.1)
Refinement
Rwork (%)15.716.320.0
Rfree (%)18.418.523.9
Rmsd from standard stereochemistry
Bond length (Å)0.0190.020.005
Bond angles (°)1.81.91.0
Ramachandran plot statistics
Most favored (%)96.998.198.5
Allowed regions (%)1.11.91.5
Disallowed regions (%)000
  • Values in parentheses refer to the highest resolution shell.

  • Rwork and Rfree factors are calculated using the formula Formula, where f(obs)hkl and f(calc)hkl are observed and measured structure factors, respectively. Rwork and Rfree differ in the set of reflections they are calculated from: Rfree is calculated for the test set, whereas Rwork is calculated for the working set.