Table 1. Suppression of colony formation by JNK1
Vectors transfectedNo. of colonies
Experiment 1Experiment 2
pSV‐Neo (2 μg)215195
pBabe‐Puro (2 μg)198209
pSV‐Neo + pCMV‐JNK1WT (1 μg)3539
pSV‐Neo + pCMV‐JNK1WT (2 μg)2216
pSV‐Neo + pCMV‐JNK1Mut (1 μg)189179
pSV‐Neo + pCMV‐JNK1Mut (2 μg)212215
pBabe‐Puro + Rb (2 μg)4542
  • Approximately 10 000 Saos‐2 cells were stably transfected with the indicated vectors. After selection in 40 μg/ml of G418 or 1 μg/ml of puromycin for 14 days, colonies of >20 cells were counted.