Table 1. Structural statistics
ΔG83 MicroROSEWT MicroROSE
Number of experimental restraints
 Distance restraints from NOEs:369371
  Within the stem and loop
   Intranucleotide166155
   Internucleotide203216
   Number of NOEs per residue2020
  Number of δ dihedral angles2829
 Number of H‐bond restraints (two per bond)3228
 Total number of experimental restraints429428
Structure calculation statistics
 Number of refined structures2020
Number of distance violations >0.2 Å02
Maximum violation0.20.27
Number of angle violation >5°1.65±0.62.5±0.6
Maximum violation (deg)8.578.77
Deviations from idealised covalent geometry
 Bonds (Å)4.7±3.7 × 10−34.7±1.6 × 10−3
 Angles (deg)2.14±0.022.13±0.02
Pairwise r.m.s.d.'s for heavy atoms (Å)
 Global1.01±0.3 Å0.84±0.26 Å
 Lower stem (G74‐A78, U98‐ C102)0.47±0.2 Å0.19±0.08 Å
 Upper stem and loop (C84‐ G93)0.25±0.1 Å0.36±0.19 Å
 Region of interest
  (U79‐U82, G94‐U97)0.49±0.2 Å0.69±0.34 Å
  (U79‐G83, G94‐U97)