Table 2. Synthetic interaction of mutations affecting OTase activity
 23°C30°C37°C
stt3‐3+++
Δost5+++
Δost3+++
Δost5 Δost3++
stt3‐3 Δost3++
stt3‐3 Δost5
  • Strains carrying the individual mutation were crossed, segregants were germinated on YPD + 1 M sorbitol medium at 23°C and tested for growth (+ or −) on YPD medium lacking sorbitol at the temperature indicated. stt3‐3 Δost5 double mutants are not viable.