Table 1. List of plasmids used in this study
PlasmidsDescriptionSource
pML63Expression cassette for GUSM.Locke
pML142Expression cassette for AVR‐PitaM.Locke
pCB980AVR‐Pita ORF in ClaI–BamHI site of pBSSK (+)Orbach et al. (2000)
pCB16460.5 kb NdeI–NotI fragment encoding AVR‐Pita176 in pET28a (+)this study
pCB1650NΔ586: Pi‐ta LRD in‐frame fusion to EcoRI–SalI of GAL4 DNA activation domain of pAD‐GAL4this study
pCB1657AVR‐Pita176 in‐frame fusion to EcoRI–SalI of GAL4 DNA‐binding domain of pBD‐GAL4this study
pCB1760Pi‐ta LRDA918S in‐frame fusion to EcoRI–SalI of pBD‐GAL4this study
pCB1761AVR‐Pita176 in‐frame fusion to EcoRI–SalI of pAD‐GAL4this study
pCB1896NΔ586: Pi‐ta LRD in‐frame fusion to EcoRI–SalI of pBD‐GAL4this study
pCB1900Pi‐ta LRD in‐frame fusion to an S‐tag in EcoRI–SalI of modified pET 29a (+)this study
pCB1902AVR‐Pita ORF in‐frame fusion to EcoRI–SalI of pAD‐GAL4this study
pCB19062.8 kb NcoI–HindIII of Pi‐ta ORF in pSL1180Bryan et al. (2000)
pCB1917NΔ247: 2.1 kb EcoRI–HindIII of Pi‐ta NΔ247 in pBD‐GAL4this study
pCB1921NΔ315: 1.8 kb EcoRI–SalI of Pi‐ta in pBD‐GAL4this study
pCB1922NΔ684: 0.7 kb EcoRI–HindIII of Pi‐ta N684 in pBD‐GAL4this study
pCB1925AVR‐Pita176 E177D in‐frame fusion to EcoRI–SalI of pAD‐GAL4this study
pCB19270.5 kb NdeI–NotI fragment encoding AVR‐Pita176M177W in pET28 (+)this study
pCB19410.5 kb NcoI–KpnI fragment encoding AVR‐Pita166 in pML142this study
pCB19470.6 kb NcoI–KpnI fragment encoding AVR‐Pita176 in pML142this study
pCB20120.6 kb NcoI–KpnI fragment encoding avr‐pita176E177D in pML142this study
pCB20130.6 kb NcoI–KpnI fragment encoding avr‐pita176M178W in pML142this study
pCB20742.8 kb EcoRI–SalI Pi‐ta ORF in‐frame fusion to pBD‐GAL4this study
pCB21110.7 kb KpnI–SpeI fragment encoding AVR‐Pita223 in pML142this study