Skip to main content
Advertisement
  • Other Publications
    • EMBO Press
    • The EMBO Journal (Home)
    • EMBO reports
    • EMBO Molecular Medicine
    • Molecular Systems Biology
    • Life Science Alliance
Login

   

Search

Advanced Search

Journal

  • Home
  • Latest Online
  • Current Issue
  • Archive
  • Subject Collections
  • Review Series & Focuses

Authors & Referees

  • Submit
  • Author Guidelines
  • Aims & Scope
  • Editors & Board
  • Transparent Process
  • Bibliometrics
  • Referee Guidelines
  • Open Access Charges

Info

  • E-Mail Editorial Office
  • Alerts
  • RSS Feeds
  • Subscriptions & Access
  • Reprints & Permissions
  • Advertise & Sponsor
  • Media Partners
  • News & Press
  • Recommend to Librarian
  • Customer Service
  • Home
  • The EMBO Journal: 36 (13)

Transparent Process

Article

PAR‐1 promotes microtubule breakdown during dendrite pruning in Drosophila

Svende Herzmann, Rafael Krumkamp, Sandra Rode, Carina Kintrup, View ORCID ProfileSebastian Rumpf
DOI 10.15252/embj.201695890 | Published online 29.05.2017
The EMBO Journal (2017) 36, 1981-1991
Svende Herzmann
Institute for Neurobiology, University of Münster, Münster, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rafael Krumkamp
Institute for Neurobiology, University of Münster, Münster, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sandra Rode
Institute for Neurobiology, University of Münster, Münster, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carina Kintrup
Institute for Neurobiology, University of Münster, Münster, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sebastian Rumpf
Institute for Neurobiology, University of Münster, Münster, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Author Affiliations

  1. Svende Herzmann1,
  2. Rafael Krumkamp1,
  3. Sandra Rode1,
  4. Carina Kintrup1 and
  5. Sebastian Rumpf (sebastian.rumpf{at}uni-muenster.de)*,1
  1. 1Institute for Neurobiology, University of Münster, Münster, Germany
  1. ↵*Corresponding author. Tel: +49 251 8322390; E‐mail: sebastian.rumpf{at}uni-muenster.de
View Full Text
  • Article
  • Figures & Data
  • Transparent Process
Loading

Abstract

Pruning of unspecific neurites is an important mechanism during neuronal morphogenesis. Drosophila sensory neurons prune their dendrites during metamorphosis. Pruning dendrites are severed in their proximal regions. Prior to severing, dendritic microtubules undergo local disassembly, and dendrites thin extensively through local endocytosis. Microtubule disassembly requires a katanin homologue, but the signals initiating microtubule breakdown are not known. Here, we show that the kinase PAR‐1 is required for pruning and dendritic microtubule breakdown. Our data show that neurons lacking PAR‐1 fail to break down dendritic microtubules, and PAR‐1 is required for an increase in neuronal microtubule dynamics at the onset of metamorphosis. Mammalian PAR‐1 is a known Tau kinase, and genetic interactions suggest that PAR‐1 promotes microtubule breakdown largely via inhibition of Tau also in Drosophila. Finally, PAR‐1 is also required for dendritic thinning, suggesting that microtubule breakdown might precede ensuing plasma membrane alterations. Our results shed light on the signaling cascades and epistatic relationships involved in neurite destabilization during dendrite pruning.

Synopsis

Embedded Image

The developmental elimination of long stretches of neurite during neuronal morphogenesis—also known as pruning—involves the disassembly of microtubules in affected neurites. Using genetic and imaging analyses of dendrite pruning in Drosophila, we found that microtubule disruption is driven by the kinase PAR‐1, likely via an inhibitory effect on Tau.

  • Mutation or knockdown of PAR‐1 leads to dendrite pruning defects.

  • Loss of PAR‐1 prevents microtubule disruption during pruning.

  • PAR‐1 increases microtubule dynamics at the onset of pruning.

  • Genetic analysis suggests Tau is the relevant PAR‐1 target.

  • PAR‐1 is also required for subsequent membrane collapse.

  • dendrite
  • PAR‐1
  • pruning
  • Tau

The EMBO Journal (2017) 36: 1981–1991

  • Received October 15, 2016.
  • Revision received April 21, 2017.
  • Accepted April 26, 2017.
  • © 2017 The Authors
View Full Text

Subscribers, please sign in with your username and password.

Log in using your username and password

Enter your The EMBO Journal username.
Enter the password that accompanies your username.
Forgot your user name or password?

Log in through your institution

You may be able to gain access using your login credentials for your institution. Contact your library if you do not have a username and password.
If your organization uses OpenAthens, you can log in using your OpenAthens username and password. To check if your institution is supported, please see this list. Contact your library for more details.

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$35.00

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

EMBO Members please login here to access the journals

Subscribe to the Journal

EMBO Journal

EMBO Reports

Recommend to your Librarian

EMBO Journal

EMBO Reports

 

 

Previous Article in this IssueNext Article in this Issue
Back to top

  • PDF
  • Share
  • Export
  • Print
Loading

PDF

Review Process

In this Issue
Volume 36, Issue 13
03 July 2017 | pp 1803 - 1991
The EMBO Journal: 36 (13)
About the cover
Alert me when this article is cited
Alert me if a correction is posted

Article

  • Article
    • Abstract
    • Synopsis
    • Introduction
    • Results
    • Discussion
    • Materials and Methods
    • Author contributions
    • Conflict of interest
    • Expanded View
    • Acknowledgements
    • References
  • Figures & Data
  • Transparent Process

Related Content

More Articles

  • Continuous signaling of CD79b and CD19 is required for the fitness of Burkitt lymphoma B cells
    Xiaocui He, Kathrin Kläsener, Joseena M Iype, Martin Becker, Palash C Maity, Marco Cavallari, Peter J Nielsen, Jianying Yang, Michael Reth
    The EMBO Journal : e97980
  • Thermal proteome profiling of breast cancer cells reveals proteasomal activation by CDK4/6 inhibitor palbociclib
    Teemu P Miettinen, Julien Peltier, Anetta Härtlova, Marek Gierliński, Valerie M Jansen, Matthias Trost, Mikael Björklund
    The EMBO Journal : e98359
  • The RNA‐binding protein Hfq is important for ribosome biogenesis and affects translation fidelity
    José M Andrade, Ricardo F dos Santos, Irina Chelysheva, Zoya Ignatova, Cecília M Arraiano
    The EMBO Journal : e97631
More Article

Related Articles

Cited By...

Request Permissions

Subject Areas

  • Cell Adhesion, Polarity & Cytoskeleton
  • Neuroscience

Journal

  • Latest Online
  • Current Issue
  • Archive
  • Bibliometrics
  • E-Mail Editorial Office

Authors & Referees

  • Aims & Scope
  • Editors & Board
  • Transparent Process
  • Author Guidelines
  • Referee Guidelines
  • Open Access
  • Submit

Info

  • Alerts
  • RSS Feeds
  • Subscriptions & Access
  • Reprints & Permissions
  • Advertise & Sponsor
  • News & Press
  • Recommend to Librarian
  • Customer Service

EMBO

  • Funding & Awards
  • Events
  • Science Policy
  • Members
  • About EMBO

Online ISSN  1460-2075

Copyright© 2018 EMBO

This website is best viewed using the latest versions of all modern web browsers. Older browsers may not display correctly.