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Linkage between the bacterial acid stress and stringent responses: the structure of the inducible lysine decarboxylase

Usheer Kanjee, Irina Gutsche, Eftichia Alexopoulos, Boyu Zhao, Majida El Bakkouri, Guillaume Thibault, Kaiyin Liu, Shaliny Ramachandran, Jamie Snider, Emil F Pai, Walid A Houry

Author Affiliations

  1. Usheer Kanjee1,
  2. Irina Gutsche2,
  3. Eftichia Alexopoulos1,3,,
  4. Boyu Zhao1,
  5. Majida El Bakkouri1,
  6. Guillaume Thibault1,,
  7. Kaiyin Liu1,
  8. Shaliny Ramachandran1,
  9. Jamie Snider1,
  10. Emil F Pai1,3,4,5 and
  11. Walid A Houry*,1
  1. 1 Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
  2. 2 Unit for Virus Host–Cell Interactions, Université Joseph Fourier/European Molecular Biology Laboratory Grenoble Outstation/Centre National de la Recherche Scientifique, Grenoble, France
  3. 3 Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
  4. 4 Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
  5. 5 Division of Cancer Genomics and Proteomics, Ontario Cancer Institute/Princess Margaret Hospital, Campbell Family Institute for Cancer Research, Toronto, Ontario, Canada
  1. *Corresponding author. Departments of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8. Tel.: +416 946 7141; Fax: +416 978 8548; E-mail: walid.houry{at}utoronto.ca
  • Present address: BBS Naturwissenschaften Ludwigshafen, Franz‐Zang‐Straße 3‐7, D‐67059 Ludwigshafen, Germany

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Abstract

The Escherichia coli inducible lysine decarboxylase, LdcI/CadA, together with the inner‐membrane lysine‐cadaverine antiporter, CadB, provide cells with protection against mild acidic conditions (pH∼5). To gain a better understanding of the molecular processes underlying the acid stress response, the X‐ray crystal structure of LdcI was determined. The structure revealed that the protein is an oligomer of five dimers that associate to form a decamer. Surprisingly, LdcI was found to co‐crystallize with the stringent response effector molecule ppGpp, also known as the alarmone, with 10 ppGpp molecules in the decamer. ppGpp is known to mediate the stringent response, which occurs in response to nutrient deprivation. The alarmone strongly inhibited LdcI enzymatic activity. This inhibition is important for modulating the consumption of lysine in cells during acid stress under nutrient limiting conditions. Hence, our data provide direct evidence for a link between the bacterial acid stress and stringent responses.

Introduction

In the early 1940s, Gale and Epps (1942) observed that Escherichia coli induced a number of amino‐acid decarboxylases in response to reduced pH and that the action of these decarboxylases resulted in the increase of pH of the growth medium. Four acid stress‐induced amino‐acid decarboxylases are responsible for this phenomenon. They include the paralogous GadA/GadB glutamic acid decarboxylases (Castanie‐Cornet et al, 1999), the inducible arginine decarboxylase AdiA (Iyer et al, 2003), the inducible ornithine decarboxylase SpeF (Kashiwagi et al, 1991), and the inducible lysine decarboxylase LdcI (also called CadA) (Meng and Bennett, 1992; Snider et al, 2006). Each of these systems has a different pH optimum and as such is capable of providing protection against acid stress over a wide range of pH values: down to pH 2.0 for extreme acid stress with GadA/B and AdiA (and to a lesser extent LdcI) and to pH 5.0 with LdcI and SpeF. The enzymes utilize pyridoxal‐5′‐phosphate (PLP) as a co‐factor to decarboxylate the α‐carbonyl group of their target amino acids in a reaction that consumes an intracellular proton (Foster, 2004). The products of decarboxylation are exchanged for fresh substrate via specific inner‐membrane antiporters.

The cytoplasmic inducible lysine decarboxylase (LdcI/CadA—SwissProt # P0A9H3) is found together with an inner‐membrane lysine‐cadaverine antiporter CadB (Soksawatmaekhin et al, 2004) on the cadBA operon. Transcription from this operon is controlled by the upstream membrane‐bound transcription activator CadC (Watson et al, 1992). Maximum cadBA expression occurs under conditions of low pH with excess lysine and anaerobiosis. We recently showed that LdcI interacts with a novel MoxR AAA+ (ATPases associated with various cellular activities) protein named RavA (Snider et al, 2006).

LdcI catalyses the proton‐dependent decarboxylation of l‐lysine to produce the polyamine cadaverine and carbon dioxide (Sabo et al, 1974). Carbon dioxide diffuses passively out of the cell and the basic cadaverine is excreted into the medium in exchange for fresh lysine by CadB. The cadBA system provides moderate protection for cells exposed to extreme acid shock (pH 2.0–2.5) (Iyer et al, 2003; Snider et al, 2006), but is important for cells growing under less extreme conditions (∼pH 5.0) (Watson et al, 1992; Dell et al, 1994). Of note, both the gadABC and cadBA systems contribute to the survival of cells grown under conditions that mimic those found in the small intestine, namely: anaerobic environment, phosphate starvation, and presence of fermentation acids (Moreau, 2007). The cadBA system also provides protection against acid stress for pathogenic enterobacteria such as Salmonella enterica serovar Typhimurium (Park et al, 1996) and Vibrio cholerae (Merrell and Camilli, 1999).

Here, we present the X‐ray crystal structure of LdcI and show that LdcI has a specific binding pocket for the stringent response regulator/alarmone ppGpp. We demonstrate that the activity of LdcI is inhibited in the presence of ppGpp in vitro and in vivo. The induction of the alarmone occurs under various stress conditions or when nutrients become limiting and this has a profound effect on cell physiology. Such effects include the downregulation of stable RNA expression and upregulation of amino‐acid biosynthesis genes through the direct binding of ppGpp to the β and β′ subunits of RNA polymerase (RNAP) (Artsimovitch et al, 2004; Potrykus and Cashel, 2008), inhibition of DNA replication (Wang et al, 2007), inhibition of protein synthesis (Svitil et al, 1993), induction of the general stress response sigma factor σS (Cashel et al, 1996), as well as, numerous pleiotropic effects that optimize the cell for survival under stress and starvation conditions.

The novel interaction that we have observed between ppGpp and LdcI is important for regulating the activity of LdcI and provides a previously unknown link between the acid stress and stringent response systems.

Results

The overall structure of LdcI

The X‐ray crystal structure of LdcI was determined to a resolution of 2.0 Å with five monomers in the asymmetric unit (refer to Alexopoulos et al (2008) for crystallographic details and to Table I for refinement statistics). The LdcI monomer can be divided into three domains (Figure 1A): an N‐terminal wing domain (residues 1–129), a core domain (residues 130–563), and a C‐terminal domain (CTD) (residues 564–715). The core domain is made up of a linker region (residues 130–183) followed by two subdomains: a PLP‐binding subdomain (PLP‐SD) (residues 184–417) corresponding to the large domain of PLP‐fold type I enzymes (Käck et al, 1999), and subdomain 4 (SD4) (residues 418–563) corresponding to the small domain of PLP‐fold type I enzymes. The wing domain adopts a flavodoxin‐like fold and belongs to the CheY‐related SCOP‐fold family (Murzin et al, 1995), which is characterized by a five‐stranded parallel β‐sheet sandwiched between two sets of amphipathic α‐helices (Figure 1B). The linker region consists of a short helical bundle (α5 – η2 – α6 – α7) that extends outwards from the PLP‐SD and is almost at right angles to the wing domain. The PLP‐SD has a seven‐stranded β‐sheet core surrounded by three sets of α‐helices. Following strand β14 of the β‐sheet core is a short α‐helix (α13) that contains the conserved lysine residue K367 that forms a covalent bond to the PLP co‐factor. SD4 stacks on top of PLP‐SD and has a central four‐stranded antiparallel β‐sheet core and the outer face has three prominent α‐helices (α16, α17, and α18), where α16 and α18 are almost parallel and form part of the oligomerisation interface. The CTD has a predominantly α‐helical outer surface and an inner surface that has two sets of β‐sheets. The CTD folds over the surface formed by PLP‐SD and SD4 and forms part of the entry channel into the active site of the enzyme.

Figure 1.

Primary sequence and structure of LdcI dimer. (A) The primary sequence of LdcI is shown with the secondary structure elements indicated above the sequence. The boundaries of the domains are indicated with boxes as follows: N‐terminal wing domain—red, linker region—orange, PLP‐SD—yellow, SD4—green; and CTD—blue. The conserved lysine (K367) that covalently binds the PLP co‐factor is highlighted in red and residues that are involved in binding to ppGpp are highlighted in blue. (B) Cartoon representation of the LdcI dimer is shown orthogonal to the molecular two‐fold axis with the domains of the top monomer coloured as in (A) and the bottom monomer coloured grey. Inserts show the secondary structure elements of each of the domains. The subdomains that are part of the core domain are indicated with bold boxes. The PLP co‐factor is shown as sticks with the co‐factor for the top monomer coloured lime green and the one for the bottom monomer (PLP′) coloured grey. For PLP, oxygen atoms are in red, nitrogen atoms are in blue, and the phosphate atoms are in cyan. Residues 712–715 were not visible in the electron density map and are not modelled.

View this table:
Table 1. Data collection and final refinement statistics for the LdcI crystal structure

The primary unit of the LdcI structure is the two‐fold symmetric dimer (Figure 1B) within which the core domains of each monomer are tightly associated, while the wing domains project outwards from the body of the dimer. The majority of the dimer interface is formed by the linker region and PLP‐SD with smaller contributions from the remaining protein domains (Supplementary Figure S1). As a consequence of dimerization, the active site of each monomer is buried and the dimer interface forms a narrow cleft that leads into the active site. Dimerization is also necessary for the completion of the active site of each monomer, a common feature in PLP‐dependent fold type I decarboxylases (Käck et al, 1999).

The LdcI decamer is ∼183 Å wide and 93 Å high and has a central pore of 25 Å diameter as well as five 9 Å wide side pores (Figure 2A). The decamer is formed by the association of five dimers to form a double‐ringed structure with distinct five‐fold symmetry as we and others previously observed by negative‐staining electron microscopy (EM) (Sabo et al, 1974; Snider et al, 2006) and during the initial determination of the X‐ray crystal structure of LdcI (Alexopoulos et al, 2008). In the current work, we obtained an ∼20 Å cryoEM reconstruction of LdcI (Figure 2B) that also clearly shows the central and side pores in the decameric structure. A rigid body fit of the X‐ray crystal structure, which was determined at pH 8.5, into the cryoEM reconstruction obtained at pH 8.0 (Supplementary Figure S2) indicates that the docking was of high quality, suggesting that the oligomeric conformation is stable and that the obtained X‐ray structure of LdcI matches the solution structure of the protein.

Figure 2.

Structure of the LdcI decamer. (A) A cartoon representation of the LdcI decamer is shown beneath a transparent van der Waals' surface. Neighbouring monomers in a dimer are colour matched except for those of the top‐most pair that are coloured as in Figure 1B. The dimensions of one of the side pores and the central pore are indicated. All scale bars are 10 Å. (B) Side and top views of the cryoEM reconstruction of the LdcI decamer to a resolution of ∼20 Å are shown. (C) The inter‐ring/dimer interface and the intra‐ring/decamer interface are shown schematically. (D) A close‐up view of the two interaction interfaces that are responsible for the association of neighbouring dimers into the decamer. Secondary structure elements from the light blue monomer are indicated with a prime symbol. Box 1 and Box 2 interaction interfaces are shown. Residue L89 that is mutated to generate an LdcI dimer (see Figure 6) is labelled. (E) Oligomerisation of AdiA decamer (PDB ID 2VYC) and OdcI dodecamer (PDB ID 1ORD). Dimers in each oligomer are colour matched. Lower panels show close‐up views of the oligomerisation interfaces. The wing domain of one grey coloured monomer is shown interacting with the neighbouring monomer in blue. The Box 1 and Box 2 interfaces are shown.

Within the context of the LdcI decamer, each monomer participates in two types of interactions: inter‐ring/dimer interactions and intra‐ring/decamer interactions (Figure 2C). The intra‐ring interactions are between surfaces of the wing and SD4 domains of neighbouring monomers and are indicated as Box 1 and Box 2 in Figure 2D. In Box 1, the interface is formed by the extended loop from β4′ to η1′ and parts of β5′ of the wing domain of one subunit (light blue in Figure 2D) and helices α16 and α18 of SD4 of the neighbouring subunit (coloured green). In Box 2, the interface is formed by residues in β2 and α2 in the wing domain of one subunit (coloured red) that interact with the N‐terminus of α1′ and the loop between β5′ and the N‐terminus of α4′ in the wing domain of the neighbouring subunit.

The X‐ray crystal structures for two other LdcI homologs have also been determined: the E. coli inducible arginine decarboxylase AdiA (PDB ID: 2VYC) (Andrell et al, 2009) and the Lactobacillus 30A ornithine decarboxylase OdcI (PDB ID: 1ORD) (Momany et al, 1995). Structurally, the core and CTDs of the three proteins are very similar. For these domains, the root mean squared deviation between LdcI and AdiA is 1.4 Å for 561 equivalent Cα atoms and between LdcI and OdcI is 1.6 Å for 558 equivalent Cα atoms. AdiA oligomerises to form decamers composed of five dimers while OdcI forms a dodecamer composed of six dimers (Figure 2E). All of the structures have the Box 1 interface in common while only the decameric proteins LdcI and AdiA possess the Box 2 interface. This might explain the higher oligomerisation state of OdcI. The Box 1 interface is analogous to the interaction interface formed between CheY, to which the wing domain is structurally related, and its interacting partners CheA, CheZ, and FliM (McEvoy et al, 1999) suggesting that the protein–protein interaction function of members of the CheY‐like fold is conserved.

LdcI active site

Members of the fold type I family of PLP‐dependent enzymes, despite their wide range of substrate specificities and divergent evolution, maintain a core set of residues and protein contacts that are critical for co‐factor binding (Käck et al, 1999; Paiardini et al, 2004). The active site of LdcI is shown in Figure 3A and B and as a schematic in Supplementary Figure S3A. The PLP co‐factor was clearly visible in the electron density and is covalently bonded to the ε‐amino group of K367 via a Schiff base to form the internal aldimine. During catalysis, the internal aldimine is converted to a lysine‐bound external aldimine via a transimination reaction (Christen and Mehta, 2001). The external aldimine tautomerizes to form the quinonoid intermediate that can undergo three possible reactions via loss of one of the substituents at the Cα position. Selectivity of the reaction product is determined by the orientation of the π‐orbitals of the Cα substituents as predicted by the Dunathan stereoelectronic hypothesis (Dunathan, 1966; Eliot and Kirsch, 2004).

Figure 3.

PLP and ppGpp‐binding sites in LdcI. (A) The top ring of an LdcI decamer is shown as a cartoon representation, where each monomer has a different colour and the bottom ring is shown in grey as a van der Waals' surface. The PLP in each active site and the ppGpp molecules are shown as van der Waals' spheres. Oxygen atoms are in red, nitrogen atoms are in blue, and phosphate atoms are in cyan. (B) The active site of one of the LdcI monomers is shown. Residues that directly interact with PLP are labelled and residues from the second monomer in the dimer that are involved in coordinating the PLP phosphate are shown in grey and labelled with a prime symbol. The grey wire‐mesh in parts B, C, and D represent the refined 2mFoDFc (Murshudov et al, 1997) electron density map contoured at the 1σ level. (C) Shown is a close‐up view of one of the ppGpp‐binding sites where the left‐hand monomer is coloured orange and the right‐hand monomer is coloured grey (labels for this monomer are indicated with a prime symbol). The position of the C5′ and C3′ carbon atoms on the ppGpp ribose ring as well as the phosphate residues are indicated in italics. (D) An alternative conformation of the 3′ β‐phosphate was observed in four of the five ppGpp molecules within each ring of the LdcI decamer and the fit of this conformer to the electron density is shown. (E) Schematic of ppGpp‐binding site generated using HBPLUS (McDonald and Thornton, 1994) and LIGPLOT (Wallace et al, 1995). Carbon atoms are shown as black spheres, oxygen atoms are shown as red spheres, nitrogen atoms are shown as blue spheres, and the phosphorous atoms are shown as cyan spheres. ppGpp is shown with purple bonds, residues from one monomer are shown with orange bonds, and residues from the neighbouring monomer are shown with grey bonds. The inset shows the lower occupancy alternative conformation that was observed for the 3′Pβ in four of the five ppGpp molecules in the LdcI pentamer. (F) Location of the ppGpp‐binding site relative to the neighbouring PLP‐binding sites in LdcI is shown. Binding of ppGpp may result in conformational changes that are communicated to the active site of the left‐hand monomer (green), potentially via helices α8′, α15′, and α16′ to helix α13′ that bears PLP′.

PLP participates in five sets of interactions with residues in the active site. (1) The phosphate group of PLP forms hydrogen bonds both with residues from the monomer to which the PLP is covalently bound (T220, S221, S364, and H366) as well as to residues from the second monomer in the dimer (T398′, T399′, and S400′). (2) The electron withdrawing ability of the pyridoxal ring is enhanced (John, 1995) by the structurally conserved salt bridge/hydrogen bond between the pyridine nitrogen N1 and the carboxylate group of D330. (3) The pyridoxal O3 oxygen atom interacts with the ring nitrogen NE1 of W333. (4) The imidazole ring of H245 stacks parallel to the re face of the pyridoxal ring. The parallel stacking of an aromatic side chain (H, Y, W, F) in this position favours the formation of the quinonoid intermediate during catalysis (John, 1995; Käck et al, 1999). (5) The methyl group of A332 forms a σ–π stacking interaction with the si face of the PLP ring consistent with the general stabilization of the si face of the ring with small amino acids (I, V, A, T, S) (Käck et al, 1999). An overlay of the active site of LdcI on that of AdiA or OdcI (Supplementary Figure S3B and C) shows that the residues coordinating PLP are highly conserved.

Analysis of the channels leading to the active sites in LdcI, AdiA, and OdcI dimers showed that LdcI had a larger channel volume compared with that of AdiA and OdcI (Supplementary Figure S4A and B). The residues involved in coordinating the substrate during decarboxylation have not been clearly identified in these enzymes. However, we observed a number of negatively charged residues that line the entry channel to the active site that may interact with the positively charged substrates (Supplementary Figure S4C and D). Residues on the lower part of the cleft are mostly from the SD4 and CT domains of one monomer while residues on the upper part of the cleft are exclusively from the PLP‐SD of the opposing monomer. Momany et al (1995) proposed that E532 may be involved in coordinating the ornithine aldimine in OdcI, but this residue, while present in LdcI as E526, is absent in AdiA. In contrast, Andrell et al (2009) proposed that D202′ and E739 in AdiA may be important in coordinating the arginine aldimine. Negatively charged residues in equivalent positions straddling the active site cleft are present in LdcI (D192′ and D692) and OdcI (D167′ and E697). In all cases, interaction with the substrate aldimine might require closure of the active site channel to bring them into close proximity. This effect has been observed most notably in E. coli aspartate aminotransferase where the residue R292 undergoes large movements upon substrate binding leading to formation of the closed complex (Kirsch et al, 1984; Ishijima et al, 2000).

LdcI binds ppGpp

During the structure refinement process, we observed that portions of the electron density could not be adequately accounted for either as part of the amino‐acid chain of LdcI or by any of the components of the crystallization mixture. After testing different small molecules, we determined that the stringent response effector molecule ppGpp provided the best fit to the electron density with generally good occupancy and reasonable average B‐factors (see Supplementary Figure S5A–D for views of the unbiased 2mFoDFc electron density maps). This was an unexpected finding indicating that ppGpp co‐purified and then co‐crystallized with LdcI. This also suggests that the interaction between LdcI and ppGpp is a specific and strong interaction (see below).

The ppGpp molecules are located at the interface between neighbouring monomers within each ring of the decamer resulting in 10 ppGpp‐binding sites per decamer (Figure 3A, C–F). The ppGpp‐binding pocket is made up of residues from SD4 and CTD of one monomer (coloured light orange in Figure 3C) and the wing domain, PLP‐SD, and SD4 of the neighbouring subunit (coloured grey and labelled with a prime symbol). The guanosine base of ppGpp is in the syn conformation and the ribose ring is in the 3′ endo conformation. Specificity for the guanosine base is provided for by three residues: the side chain of R558 hydrogen bonds with O6 and N7 of the guanosine base; the guanidinium group of R97′ stacks against one face of the guanosine base, and the side chain of L564 forms a σ–π interaction with the opposite face.

The ppGpp‐binding pocket has a number of positively charged residues that bind to the phosphate groups of ppGpp. The majority of the interactions are with the 5′α‐ and 5′β‐phosphates, where every oxygen atom except the phospho‐esterified oxygen atom participates in an interaction (Figure 3C and E). The 5′α‐phosphate interacts with the side chain of R585 and N568, as well as with the backbone amide of R565. The 5′β‐phosphate interacts with the side chain of R585, the side chain of R565, and the side chain of R206′. Comparison of the residues in equivalent positions to the LdcI ppGpp‐binding site in AdiA and OdcI showed that 3 of the 10 residues are strictly conserved (K417, G418, and R206) (Supplementary Figure S5E and F).

In four of the five ppGpp‐binding sites within each ring of the LdcI decamer, two conformations for the 3′β‐phosphate were observed. In the primary conformation of the 3′β‐phosphate, observed in all five ppGpp‐binding sites (Figure 3C and E), the 3′β‐phosphate forms hydrogen bonds to both the side chain of R206′ and the backbone amide of G418′. The secondary conformation (Figure 3D and inset in E) was observed in four of the five binding sites, and the electron density around this phosphate was generally weaker, indicating that this site was occupied less frequently compared with the primary site. Due to the change in position of the 3′β‐phosphate, the hydrogen bonds with R206′ and G418′ are lost while a new hydrogen bond is formed with the side chain of K417′. The ppGpp molecule also interacts with a number of water molecules, but none of these interactions are spatially conserved among the five binding sites.

The fact that the ppGpp‐binding site was found at an interface between two LdcI protomers and has contributions from residues from several domains led us to speculate that the alarmone might modulate LdcI activity and/or oligomerisation.

LdcI activity is inhibited by the alarmone

To determine whether ppGpp has an effect on LdcI activity, we purified apo‐LdcI from a ppGpp0 strain and tested the activity of the apoenzyme (at a concentration of 25 nM) at different pH values (Figure 4A). In the absence of any nucleotide, LdcI has an activity maximum at pH 5.5 corresponding to an activity of 234 (nmoles cadaverine produced) min−1 (μg LdcI)−1. Addition of ppGpp at low salt concentrations (25–135 mM NaCl depending on the buffer) resulted in a dramatic inhibition of LdcI activity of ∼10‐fold at pH values higher than 5.0 (Figure 4A, upper panel). The pentaphosphate pppGpp also inhibited LdcI but only at pH values higher than 6.5. The related nucleotides GDP and GTP were ineffective at inhibiting LdcI, indicating that there is a high degree of specificity for (p)ppGpp. At pH values lower than 5.0, there is no effect of any nucleotide on LdcI activity.

Figure 4.

Inhibition of LdcI activity by (p)ppGpp. (A) LdcI (25 nM) activity was measured from pH 4.5 to 8.5 in the absence or presence of 100 μM of nucleotides under low salt (25–135 mM NaCl, upper panel) or high salt (⩾300 mM NaCl, lower panel) conditions. Error bars represent the s.d. for the measurements of three repeats. (B) Isothermal titration calorimetry was used to measure ppGpp binding to LdcI at pH 6.5. Data were fit to a binding model with two non‐identical independent binding sites. The inset shows the derived number of binding sites, Kd, ΔH, and ΔS for each type of binding site. (C) Kinetics of LdcI inhibition by ppGpp were followed at pH 6.5 using an isothermal titration calorimetry protocol. Error bars represent the s.d. for the measurements of three repeats. The inset shows the results of the fit of the data. The values in parentheses indicate the standard error from the calculated fits and NA indicates not applicable.

For free ppGpp, the physiological pKa values of the 3′β‐ and 5′β‐phosphates were calculated using MARVIN (ChemAxon, 2009) to be 6.52 and 6.82, respectively. Hence, as the pH increases above these pKa values, the overall charge of ppGpp becomes more negative resulting in a tighter interaction with LdcI. In addition, the effective range of inhibition was lowered when higher concentrations of either NaCl (⩾300 mM) (Figure 4A, lower panel) or KCl (⩾300 mM) (Supplementary Figure S6) were used in the assay, indicating that the binding of ppGpp to LdcI has a strong ionic character. Moreover, the identity of the cation (Na+ or K+) did not affect the activity of LdcI or the inhibition by ppGpp (Supplementary Figure S6).

In order to determine the stoichiometry of binding of ppGpp to LdcI, we performed isothermal titration calorimetry (ITC) at pH 6.5 (Figure 4B). For each LdcI decamer, we observed a total of 10 binding sites with 5 high affinity sites (Kd∼13 nM) and 5 lower affinity binding sites (Kd∼685 nM). To explore the mechanism of inhibition, we used an ITC‐based enzyme assay to follow the effect of ppGpp on LdcI activity over a range of lysine concentrations (Figure 4C). Apo‐LdcI exhibits typical Michaelis–Menten kinetics with a KM of 0.42 mM, kcat of 1.80 × 103 min−1, and a kcat/KM of 4.26 × 106 M−1 min−1. In the presence of ppGpp, the kinetics (Figure 4C) can be modelled to simple inhibition mechanisms that generate comparable kinetic parameters to apo‐LdcI with Ki values in the nM to μM range (Figure 4C). This suggests that LdcI will be effectively inhibited in vivo at the physiological concentrations of ppGpp (in the mM range, also see below) reached during the stringent response (Cashel et al, 1996).

LdcI oligomerisation is modulated by pH and concentration

We utilized a number of techniques to probe the oligomerisation of LdcI as a function of pH and protein concentration. LdcI at 1 μM concentration was run on a size exclusion chromatography (SEC) column at different pH values. At pH 5.0, large aggregates that elute in the void volume of the column were observed (exclusion limit of the column is ∼40 000 kDa) while at pH 6.5 and 8.0 decamers were dominant (Figure 5A). Sedimentation velocity analytical ultracentrifugation experiments of 25 μM LdcI at pH 8.0 yielded similar results, while experiments at pH 6.5 showed a mixture of LdcI decamers and larger species (Supplementary Figure S7A). Negative‐staining EM on ∼400 nM LdcI (Figure 5B, top row) shows that, at pH 5.0, decamers and higher‐order oligomers are present while, at pH 6.5 and 8.0, decamers and an increasing proportion of dimers are present. If we assume equal binding of decamers and dimers to the grid, based on the analysis of 35 000 particles, we estimate that at pH 6.5 there are ∼25% dimers and 75% decamers while at pH 8.0 the proportion of dimers increases to 95%. Glutaraldehyde cross‐linking of 25 nM LdcI at various pH values (Supplementary Figure S7B) shows a similar pattern of large oligomers at pH 5.0 and a mixture of dimers and decamers at higher pH values.

Figure 5.

The effect of pH and ppGpp on LdcI oligomerisation. (A) Size exclusion chromatography using a Superose 6 HR 10/30 column of 1 μM LdcI at different pHs. The top row indicates 1 ml elution fractions and the positions of molecular weight standards are indicated schematically along the bottom. Each panel represents a slice from a silver‐stained polyacrylamide gel. (B) Negative‐staining EM of about 400 nM LdcI at pH 5.0, 6.5, and 8.0 is shown in the absence (top row) and presence (bottom row) of 100 μM ppGpp. Decamers are indicated by black arrowheads, dimers are indicated by white arrowheads, and higher‐order oligomers are indicated by white bordered black arrowheads. The scale bar is 20 nm. (C) Percent inhibition of activity of 20 μM LdcI at pH 5.0, 6.5, and 8.0 by 250 μM ppGpp. Error bars represent the s.d. for the measurements of three repeats. (D) Size exclusion chromatography of 20 μM LdcI on a Superose 6 HR 10/30 column at pH 5.0, 6.5, and 8.0 in the absence and presence of 100 μM ppGpp. Each panel represents a slice from a Coomassie‐stained polyacrylamide gel. (E) For selected fractions, the activity of the protein was measured using the TNBS‐based LdcI assay either in the absence (grey bars) or presence (white bars) of 100 μM ppGpp. Error bars represent the s.d. for the measurements of three repeats.

As the cellular concentration of LdcI increases to about 20 μM upon mild acid stress (see below), we additionally tested the oligomerisation and activity at this high‐protein concentration. Figure 5C shows that indeed significant inhibition of 20 μM LdcI is observed at pH 6.5 and 8.0 but not at pH 5.0, consistent with the results from enzyme assays conducted at lower protein concentrations (Figure 4A). The SEC profile of 20 μM LdcI (Figure 5D) shows that at pH 5.0, a range of large oligomeric species are present and that addition of 100 μM ppGpp shifts the distribution towards more decamers, consistent with the EM observations at lower enzyme concentrations (Figure 5B). The addition of ppGpp had no effect on the oligomerisation at either pH 6.5 or pH 8.0, where a predominance of LdcI decamers was observed. At 20 μM LdcI, no significant accumulation of LdcI dimers was visible at any of the pH values tested. The activities of selected fractions (Figure 5E) from the SEC experiment at 20 μM LdcI indicated that at pH 6.5 and 8.0, the decamer is the species that is inhibited by ppGpp. At pH 5.0, the protein activity is distributed over a range of large oligomeric species and these species are not subject to inhibition by ppGpp.

Together, these data suggest that (1) at pH 6.5 and 8.0, LdcI decamer formation is favoured as protein concentration increases and that, at cellular concentrations, LdcI is found predominantly as a decamer. (2) At low protein concentrations, LdcI has a pH‐dependent dissociation from decamers to dimers. Class averages of the LdcI dimers in solution show that the overall conformation of the dimers closely matches the conformation that we determined in the crystal structure (Supplementary Figure S7C). This suggests that dimers free in solution maintain a conformation that favours decamer formation. (3) LdcI forms higher‐order oligomers at pH 5.0, the size of which is affected by ppGpp but not their activity. Hence, this suggests that, while ppGpp can bind to LdcI stacked oligomers at pH 5.0, it is unable to promote the inhibited conformation of the enzyme at this pH. (4) At pH 6.5 and 8.0 and low protein concentrations, the addition of 100 μM ppGpp was seen to increase the proportion of decamers (Figure 5B, bottom row). This apparent stabilization of decamers by ppGpp is consistent with the location of the binding site of ppGpp at the interface between neighbouring monomers in the decamer.

Hence, pH and concentration have an important role in modulating LdcI oligomerisation and activity.

The effect of alarmone binding to LdcI on the lysine‐dependent E. coli acid stress response

Subsequently, we carried out experiments to determine the significance of ppGpp binding to LdcI on the acid stress response in E. coli. To address this question, we first generated two LdcI mutants at the ppGpp‐binding interface, R97A and R206S (Figure 3C and D) as well as a mutant that formed a dimer‐only version of LdcI, L89R (Figure 2D). The oligomerisation of the different mutants was tested by SEC at pH 6.5 (Figure 6A). The ppGpp‐binding site mutants R97A and R206S showed wild‐type oligomerisation profiles while L89R eluted at the expected position for an LdcI dimer (about 150 kDa).

Figure 6.

ppGpp modulates the activity of LdcI in the cell. (A) Wild‐type and mutant proteins at 1 μM were separated on a size exclusion column at pH 6.5 to assess the oligomeric state of LdcI. MW standards are indicated at the bottom. (B) Lysine decarboxylation activity was measured using the TNBS‐activity assay in the absence and presence of 100 μM ppGpp for WT LdcI and its different mutants at 25 nM. Rates are relative to the maximum rate obtained at pH 5.5 (Figure 4A) and error bars indicate the s.d. of the data from three repeats. The top panel shows data at pH 5.0 and the bottom panel shows data at pH 6.5. (C) Wild‐type and ΔcadBA knockout strains were grown in defined rich media containing 30 mM l‐lysine at pH 5.0 until mid‐log phase (pre‐shift), then the cells were shifted to minimal media also containing 30 mM l‐lysine weakly buffered at pH 5.0 (post‐shift). The OD600 of the cells is shown in the top panel, the pH of the culture media is shown in the middle panel, and the amount of ppGpp extracted from the cultures is shown in the bottom panel. The inset shows an HPLC trace demonstrating the induction of ppGpp in the ΔcadBA pcadBA‐R97A strain post‐shift. These experiments were repeated three times.

The decarboxylation activities of the proteins were then tested at pH 5.0 and 6.5 in the absence and presence of 100 μM ppGpp (Figure 6B). At pH 5.0, none of the proteins was inhibited by ppGpp as was observed for WT LdcI. At pH 6.5, WT LdcI was strongly inhibited by ppGpp while the R97A and R206S mutants were insensitive to the addition of ppGpp and had activities comparable to WT LdcI in the absence of ppGpp. The dimeric mutant of LdcI (L89R) showed about five‐fold lower level of activity than WT and this activity was not inhibited by ppGpp. A similar trend was also observed for an additional dimeric mutant (L547R) (Supplementary Figure S8). Hence, LdcI needs to be a decamer in order to bind ppGpp.

To determine the significance of the effect of ppGpp on LdcI activity in vivo, an approach was developed whereby the activity of LdcI in cells was determined by monitoring the change in pH of weakly buffered media containing l‐lysine when cells undergo a stringent response. The following strains were generated and tested: WT MG1655 p322, ΔcadBA p322, ΔcadBA pcadBA, ΔcadBA pcadBA‐R97A, ΔcadBA pcadBA‐R206S, and ΔcadBA pcadBA‐L89R. p322 is a pET‐based plasmid that we constructed (see Supplementary data), while pcadBA refers to the p322 plasmid containing the cadBA operon. The mutations refer to mutations in LdcI.

Cells were grown under LdcI inducing conditions in defined rich media strongly buffered at pH 5.0 and supplemented with all the amino acids plus 30 mM l‐lysine. At mid‐log phase, the cells were harvested, washed, and re‐suspended in minimal media lacking amino acids but supplemented with 30 mM l‐lysine to induce a stringent response and weakly buffered at pH 5.0. The activity of LdcI and the levels of ppGpp were then followed as a function of time (Figure 6C). As expected, the ΔcadBA strain did not show an increase in the pH of the media as it lacks the LdcI system but the mutant strains in which LdcI is not inhibited by ppGpp (ΔcadBA pcadBA‐R97A, ΔcadBA pcadBA‐R206S, and ΔcadBA pcadBA‐L89R) all displayed a more rapid rate of pH increase than both the WT and ΔcadBA pcadBA strains. ppGpp was only detectable post‐shift and, within the errors of such measurements, the levels were comparable between the different strains and did not change significantly post‐shift (Figure 6C). In addition, the amount of LdcI protein as detected by western blot was generally similar among the different strains (Supplementary Figure S9A and B). To confirm that the difference in the in vivo activity between the LdcI wild type and mutants was dependent on ppGpp, we repeated this shift experiment in a ΔrelA ΔspoT ΔcadBA strain complemented with the same plasmids as described above. Deletion of relA and spoT genes renders the strain not capable of generating ppGpp (Xiao et al, 1991). In this case, the wild‐type and mutant strains displayed an identical rate of pH change (Supplementary Figure S10).

It is interesting to note that the in vivo activity of all the LdcI ppGpp‐binding site and dimer mutants were similar (Figure 6C; Supplementary Figure S10), even though the LdcI R97A and R206S mutants have about three‐fold higher activity than the LdcI dimer mutant L89R when measured in vitro (Figure 6B). This suggests that, under the conditions tested in vivo, such differences cannot be measured or that there are other factors that may have limited the observed activity of the proteins such as saturation of the inner‐membrane lysine‐cadaverine antiporter CadB.

The data in Figure 6C indicate that the activity of LdcI in the WT and ΔcadBA pcadBA strains was inhibited by the presence of ppGpp when the cells underwent a stringent response. Hence, it could be speculated that, under nutrient limiting conditions, ppGpp is used by the cells to limit LdcI activity upon recovery from acid stress to possibly prevent the over‐consumption of lysine.

Discussion

Our unexpected finding of the modulation of LdcI activity by ppGpp through the direct binding to a specific pocket on the enzyme provides, for the first time, a link between the bacterial acid stress response and the stringent response. It is known that the major effects of the stringent response include the downregulation of stable RNAs, reduction in cellular growth rate, and induction of stress response and amino‐acid biosynthesis genes that occur as a result of the change in the transcription profile of RNAP caused by the binding of ppGpp to RNAP (Potrykus and Cashel, 2008). (p)ppGpp is also capable of exerting an effect through direct interaction with targets other than RNAP (Cashel et al, 1996), although, generally, the molecular mechanism by which the alarmone exerts its influence on other proteins is not known.

We found that the LdcI decamers have two types of ppGpp‐binding sites—low and high affinity sites (Figure 4B). This is surprising from the point of view of the structure as the five ppGpp‐binding sites in the asymmetric unit are very similar. However, we do observe two conformations for ppGpp in the binding site, one at higher occupancy and the other at lower occupancy (Figure 3C–E). Binding in the lower occupancy site could be correlated with weaker binding, but other explanations are also possible and might reflect allostery of binding. It should be noted that, when pppGpp was modelled into the binding site, we observed that the 5′γ‐phosphate was not well supported in the difference map and made few hydrogen bonds with amino acids in the binding site. Combined with the observation that pppGpp inhibits LdcI less efficiently (Figure 4A), this suggests that ppGpp is the preferred inhibitor.

It is not clear at this stage whether other decarboxylases would also be inhibited by the alarmone. However, the fact that many of the related amino‐acid decarboxylases (AdiA, SpeF, SpeC, LdcC) in E. coli are known to form oligomers suggests that the inhibition by ppGpp might be a general method for regulating these enzymes, some of which participate in the acid stress response and some of which are critical for production of polyamines. Further experimentation will be needed to establish this link.

In order to determine the effects of ppGpp binding on the LdcI structure, we crystallized apo‐LdcI. So far, the diffraction pattern from the best crystal only extends to 4.1 Å (Supplementary Figure S11; Supplementary Table S1). At this resolution, we would be able to observe only large‐scale conformational changes between LdcI‐ppGpp and apo‐LdcI or major rearrangements of the active site or ppGpp‐binding site. However, no such differences have been seen. Given the uncertainties in the placement of side‐chain groups at this resolution, we cannot comment on the possibility that ppGpp binding induces subtle changes that lead to inhibition of LdcI. As binding of the alarmone does cause inhibition, we speculate that upon ppGpp binding, smaller structural changes may be transduced via helices α8, α15, and α16 to helix α13 that bears PLP (Figure 3F). We are undertaking a systematic mutational and enzymatic analysis coupled with obtaining the structure of both the apoenzyme and holoenzyme at different pHs in order to gain a more detailed understanding of the mechanism of ppGpp inhibition.

In vitro, LdcI is seen to adopt a number of different oligomeric states depending on protein concentration and pH: namely, higher‐order stacked oligomers, decamers, and dimers. These diverse oligomeric states of LdcI exist under different conditions, have different activities, and are differently affected by ppGpp. At high‐protein concentrations, about tens of μM, LdcI forms stacked oligomers at pH 5 or lower and mainly decamers at pH 6.5 or higher, but no significant percentage of dimers are observed at these high‐enzyme concentrations (Figure 5D; Supplementary Figure S7A). At low LdcI concentrations, <1 μM, the enzyme forms stacked oligomers at pH 5 or lower, while, at pH 6.5 or higher, decamers and dimers are observed with the percentage of dimers increasing as pH increases (Figure 5A and B; Supplementary Figure S7B). Hence, there are multiple mechanisms by which the cell can regulate LdcI activity to either counter acid stress or to facilitate recovery from acid stress.

Under mild acid stress conditions with an external pH of 4–5, the internal pH inside the cell is expected to be 6–7 (Richard and Foster, 2004); under these conditions, LdcI will be highly induced (i.e. will be at high concentrations) and the decameric form of the enzyme will be the major species. If cells reach stationary phase or face starvation, then ppGpp is induced and LdcI decamer activity will be inhibited. Furthermore, as the cells recover from acid stress, LdcI levels will decrease as transcription from the cadBA operon is abolished (Watson et al, 1992) halting LdcI production and the protein concentration will be reduced due to cell division or degradation activity. As LdcI concentration is reduced and the internal pH is increased, the proportion of dimers is expected to increase as suggested by the experiments of Figure 5B. Based on the measured activity of the two dimer‐only mutants, L89R (Figure 6B) and L547R (Supplementary Figure S8B), dimers have lower specific activity than decamers. We expect this lower activity of LdcI dimers to be important for cells recovering from acid stress in the absence of a stringent response. This should eventually result in the reduction of LdcI activity and stop the consumption of lysine by the enzyme. The cellular lysine pool would then be fully available for other functions such as protein synthesis.

Under extreme acid stress conditions with an external pH of 2–3 (Richard and Foster, 2004) or in the presence of organic acids (Wilks and Slonczewski, 2007), the internal pH will be 4–5. LdcI is not expected to be significantly induced under such conditions. However, if LdcI is present under these conditions, whether at low or high concentrations, it is expected to form higher‐order oligomers composed of stacks of decamers (Figure 5A, B, and D). At this pH, LdcI is insensitive to ppGpp (Figures 4A and 5E) and this may be an important adaptation to allow LdcI to function, albeit at a reduced activity, when the internal pH of the cytoplasm falls to below pH 5 (i.e. external pH of about 3) during severe acid challenges.

The above scenarios reveal a very interesting dilemma. If cells are faced with acidic stress under nutrient limiting conditions, then high levels of amino‐acid decarboxylases such as LdcI are required to raise a proper response; however, at the same time, cells need to conserve the amount of amino acids consumed during the decarboxylation reactions and to quickly shut down such enzymes when the pH approaches neutral. The use of ppGpp to allosterically inhibit enzymatic decarboxylation activity seems to be a very efficient method to fine‐tune the bacterial acid stress response. It should be noted in this regard that, since the weaker Kd for ppGpp binding to LdcI is 685 nM, we expected LdcI to be completely inhibited during the stringent response when the concentration of ppGpp is increased to ∼1–2 mM (Figure 6C), the concentration of LdcI is ∼20–30 μM (Supplementary Figure S9B) and the cytoplasmic pH is expected to be close to neutral (Wilks and Slonczewski, 2007). Under these conditions, we expect LdcI to be predominantly decameric and to have its activity regulated primarily by binding ppGpp. Indeed, we do observe lower LdcI activity for the WT protein versus the ppGpp‐binding site mutants in vivo (Figure 6C); however, the incomplete inhibition of the protein suggests the possibility of additional systems that might regulate the inhibition of LdcI by ppGpp binding. Hence, we suspect the presence of other systems that might fine‐tune the activity of LdcI.

Materials and methods

Biochemical assays

The activity of lysine decarboxylase was measured using either a 2,4,6‐trinitrobenzensulfonic acid (TNBS) assay as described (Snider et al, 2006; Kanjee and Houry, 2010) or via ITC (using a Microcal VP‐ITC) to measure the heat of the reaction of lysine decarboxylation under a range of substrate and inhibitor concentrations. The VP‐ITC was used to measure the binding of ppGpp to LdcI following standard procedures.

SEC experiments were performed in 100 mM buffer (sodium acetate for pH 5.0, sodium MES for pH 6.5, and sodium HEPES for pH 8.0), 300 mM NaCl, 0.1 mM PLP, and 1 mM DTT and run on either an ÄKTA FPLC (GE) or a Biologic DuoFlow (Bio‐Rad) chromatography system and all experiments were performed between 4 and 10°C. Protein absorbance was determined at 280 nm and fraction sizes were 1 ml unless otherwise noted. Samples were run on either a Superose 6 HR 10/30 column (GE) or a Superdex 200 10/300 GL column (GE).

In vivo assays

The effect of binding of ppGpp to LdcI was measured in vivo as follows. Cells were grown to an OD600 of between 0.4 and 0.5 with shaking at 37°C in defined rich media (Neidhardt et al, 1974) modified as follows: media were supplemented with 40 mM MES and adjusted to pH 5.0 with KOH; the concentration of all 20 amino acids was 50 mg ml−1; the carbon source was 0.1% (w/v) d‐glucose and the media were supplemented with 30 mM l‐lysine. At mid‐log phase, cells were pelleted by centrifugation at 4000 g at 22°C for 10 min, washed once in the post‐shift media (5 mM MES pH 5.0, 1.32 mM K2HPO4, 0.2 mM each of adenine, cytosine, guanine, and uracil, and 0.001% (w/v) d‐glucose), pelleted again as above and finally re‐suspended to an OD600 of 1.0 in post‐shift media containing 30 mM l‐lysine. The cells were incubated at 37°C with shaking for 2 h and samples were withdrawn every half an hour for determination of the amount of LdcI and ppGpp and measurement of the OD600 and pH. The extraction of ppGpp from the cell culture was performed as described (Buckstein et al, 2008) with minor modifications.

Other details and biochemical protocols are provided in Supplementary data.

LdcI crystallization and structure determination

The purification, crystallization, data collection, and preliminary structure determination of LdcI‐ppGpp have been described previously (Alexopoulos et al, 2008). A detailed description of the structure determination process is presented in Supplementary data. We obtained initial low‐resolution maps at 4.5 Å by single isomorphous replacement with anomalous scattering (SIRAS) using LdcI crystals soaked in the heavy atom cluster hexatantalum dodecabromide (Ta6Br122+). The initial electron density maps were not of sufficient quality to trace the protein backbone but enabled us to generate a molecular replacement (MR) search model based on the structure of the orthologous Lactobacillus 30A ornithine decarboxylase OdcI (PDB ID: 1ORD) that shares 31.4% sequence identity with LdcI and has a similar domain architecture. We obtained an MR solution in PHASER (McCoy et al, 2007) consisting of five monomers in a star‐like arrangement. The initial refinement was started in REFMAC (Murshudov et al, 1997) using the Native‐1 data set (Alexopoulos et al, 2008) to 2.57 Å resolution, and the refinement was extended to 2.0 Å with the current high‐resolution data set (Table I). Five percent of the reflections were randomly selected as an Rfree set for cross‐validation. Initial rigid body refinement was followed by a refinement using tight non‐crystallographic symmetry restraints. Model building was performed interactively using COOT (Emsley and Cowtan, 2004). During the final refinement steps, the tight NCS restraints were released. Final refinement statistics are given in Table I. The coordinates have been deposited in the RCSB with PDB ID 3N75. All structure figures were prepared in PyMOL (DeLano, 2002). The structure determination of apo‐LdcI is described in Supplementary data with the respective coordinates deposited in the RCSB with PDB ID 3Q16.

Electron microscopy

CryoEM reconstruction of LdcI was performed on purified protein as follows. The vitrified specimen was prepared on holey carbon copper grids as described (Dubochet et al, 1988) and observed with a Phillips CM200 transmission electron microscope with a LaB6 filament at 200 kV. Images were recorded under low electron dose conditions on Kodak SO‐163 films and negatives were digitized with a Nikon Coolscan 6000 scanner at 3.34 Å pixel size at the specimen level. The defocus of the images used for further analysis was ∼2–2.5 μm as determined from the power spectra. Image processing was carried out using the EMAN software package (Ludtke et al, 1999) for particle selection, CTFFIND (Mindell and Grigorieff, 2003) for CTF determination and correction, Imagic‐5 (van Heel et al, 1996) for classification and angular reconstitution and EMAN refine procedure as an alternative 3D‐reconstruction option. No matter whether our previously described 3D reconstruction based on negative‐stain images of the LdcI decamer (Snider et al, 2006) was used as an initial model or whether ab initio calculations were performed, the final reconstruction was the same. For the purpose of this work and because of the notable similarity with the X‐ray crystal structure, we filtered this reconstruction to 20 Å resolution and did not attempt to improve it further.

For preparation of negatively stained LdcI, protein solution was applied to the clear side of carbon on a carbon–mica interface and stained with 2% (w/v) silicotungstate. The pH of the stain was adjusted with NaOH to be 5.0, 6.5, or 8.0 to correspond exactly to the pH of the sample buffer. Images were recorded under low‐dose conditions with a JEOL 1200 EX II microscope at 100 kV.

Supplementary data

Supplementary data are available at The EMBO Journal Online (http://www.embojournal.org).

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary Information

Supplementary Figure S1 [emboj20115-sup-0001.pdf]

Supplementary Figure S2 [emboj20115-sup-0002.pdf]

Supplementary Figure S3 [emboj20115-sup-0003.pdf]

Supplementary Figure S4 [emboj20115-sup-0004.pdf]

Supplementary Figure S5 [emboj20115-sup-0005.pdf]

Supplementary Figure S6 [emboj20115-sup-0006.pdf]

Supplementary Figure S7 [emboj20115-sup-0007.pdf]

Supplementary Figure S8 [emboj20115-sup-0008.pdf]

Supplementary Figure S9 [emboj20115-sup-0009.pdf]

Supplementary Figure S10 [emboj20115-sup-0010.pdf]

Supplementary Figure S11 [emboj20115-sup-0011.pdf]

Supplementary Methods [emboj20115-sup-0012.doc]

Acknowledgements

We thank Dr Michael Cashel (National Institutes of Health, Bethesda, MD) for sending us bacterial strains, plasmids, and necessary protocols. We thank Dr Eric Massé (University of Sherbrooke, Québec City, Canada) for the gift of purified P1 phage. We thank Dr Tyrell Conway (University of Oklahoma, OK) for the gift of the MG1655 ΔrelA ΔspoT strain. UK is the recipient of a National Sciences and Engineering Research Council of Canada (NSERC) Postgraduate Scholarship, a Fellowship of the Canadian Institutes of Health Research (CIHR) Strategic Training Program in the Structural Biology of Membrane Proteins Linked to Disease, and a University of Toronto Open Fellowship. EA is the recipient of a fellowship from the CIHR Strategic Training Program in Protein Folding: Principles and Diseases. BZ is the recipient of an NSERC Undergraduate Student Research Award (NSERC‐USRA). KY is the recipient of a Life Sciences Award from the University of Toronto. MEB is the recipient of a fellowship from the CIHR Strategic Training Program in Protein Folding and Interaction Dynamics: Principles and Diseases. SR is the recipient of an NSERC‐USRA and the University of Toronto Excellence Award in Natural Sciences and Engineering. GT and JS are recipients of an NSERC Postgraduate Scholarship. This work was supported by a grant from the CIHR (MOP‐67210) to WAH, by the Canada Research Chairs Program and the Ontario Research and Development Challenge Fund to EFP and in part by the Ontario Ministry of Health and Long Term Care. The views expressed do not necessarily reflect those of the OMOHLTC. The results shown in this manuscript are derived from work performed at Argonne National Laboratory, beamline 19‐BM‐D, Structural Biology Center and beamline 14‐BM‐C, BioCARS sector, both at the Advanced Photon Source. Argonne is operated by UChicago Argonne, LLC, for the US Department of Energy, Office of Biological and Environmental Research under contract DE‐AC02‐06CH11357. The use of the BioCARS Sector 14 was supported by the National Institutes of Health, National Center for Research Resources under grant No. RR0077‐7. We also thank the members of Canadian Light Source (CLS) beamline staff at CMCF‐08ID‐1 for assistance with data collection.

Footnotes

  • Current address: Temasek Life Sciences Laboratory, University of Singapore, 117604, Singapore

References

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