Alzheimer's disease (AD) is the most common form of dementia and associated with progressive deposition of amyloid β‐peptides (Aβ) in the brain. Aβ derives by sequential proteolytic processing of the amyloid precursor protein by β‐ and γ‐secretases. Rare mutations that lead to amino‐acid substitutions within or close to the Aβ domain promote the formation of neurotoxic Aβ assemblies and can cause early‐onset AD. However, mechanisms that increase the aggregation of wild‐type Aβ and cause the much more common sporadic forms of AD are largely unknown. Here, we show that extracellular Aβ undergoes phosphorylation by protein kinases at the cell surface and in cerebrospinal fluid of the human brain. Phosphorylation of serine residue 8 promotes formation of oligomeric Aβ assemblies that represent nuclei for fibrillization. Phosphorylated Aβ was detected in the brains of transgenic mice and human AD brains and showed increased toxicity in Drosophila models as compared with non‐phosphorylated Aβ. Phosphorylation of Aβ could represent an important molecular mechanism in the pathogenesis of the most common sporadic form of AD.
The post‐translational modification by phosphorylation has an important role in the regulation of protein activity. Protein kinases that catalyse the phosphorylation reaction mainly exert their activity towards intracellular targets and thereby regulate important physiological and pathophysiological processes, including cellular metabolism, differentiation and proliferation. In addition to intracellular protein kinases, several kinase activities have been demonstrated at the cell surface and in extracellular fluids (Chen et al, 1996; Redegeld et al, 1999). These ecto‐protein kinases phosphorylate cell surface localized as well as extracellular soluble proteins, including proteins of the complement system, coagulation factors and cell adhesion proteins. Several distinct ecto‐protein kinases have been characterized and appear to be very similar to intracellular kinases, such as protein kinases (PK) A, C, CK1 and CK2 (Kübler et al, 1989; Walter et al, 1996). However, the physiological and/or pathophysiological significance of extracellular protein phosphorylation is poorly understood.
Alzheimer's disease (AD) is the most common form of dementia and associated with the progressive accumulation of amyloid β‐peptides (Aβ) in form of extracellular amyloid plaques in the human brain. Aβ derives from the amyloid precursor protein (APP) by proteolytic processing. The sequential cleavage of APP by enzymes called β‐ and γ‐secretase leads to secretion of Aβ from cells into extracellular fluids (Selkoe, 2001; Mattson, 2004). Aβ peptides could aggregate and form insoluble fibrils that represent major components of extracellular plaques. The role of plaque deposition in the pathogenesis of AD and particularly their neurotoxic properties are currently under debate. However, a close relationship of plaques and impaired dendritic activity supports the critical role of plaques in neurotoxicity (Spires‐Jones et al, 2007; Koffie et al, 2009; Meyer‐Luehmann et al, 2009). Notably, recent research also strongly supports an important role of small oligomeric forms of Aβ in neurotoxicity and degeneration (Yankner, 1996; Chiti and Dobson, 2006; Haass and Selkoe, 2007; Selkoe, 2008).
A critical role of Aβ in the pathogenesis of AD is strongly supported by gene mutations that cause early‐onset familial forms of the disease. Such mutations have been identified in the APP gene itself and in presenilin 1 and 2. Importantly, all mutations identified in the different genes commonly lead to early deposition of extracellular plaques likely by increasing the generation and/or aggregation of Aβ (Nilsberth et al, 2001; Kennedy et al, 2003; Tanzi and Bertram, 2005; Hori et al, 2007; Di et al, 2009).
The aggregation of Aβ and other proteins that cause neurodegenerative and other diseases appears to follow similar pathways and depends on the formation of small soluble nuclei that could act as seeds and thereby promote rapid fibril growth (Harper and Lansbury, 1997; Soto and Estrada, 2008). Thus, the assembly of monomeric proteins or peptides into smaller oligomeric structures is the rate‐limiting step in fibril formation. Certain mutations within the Aβ domain that cause early‐onset AD (EOAD) promote formation of oligomeric assemblies and fibrillization (Kirkitadze et al, 2001; Murakami et al, 2003; Tomiyama et al, 2010). However, such disease causing mutations in the Aβ domain are very rare and only account for a few cases of EOAD.
Mechanisms that drive formation of oligomeric nuclei of wild‐type (WT) Aβ and thereby might promote the pathogenesis of AD remain largely unclear. However, studies on pyroglutamate‐modified variants of Aβ suggest a critical role of N‐terminal modification in the aggregation process (Schilling et al, 2008). Whether other post‐translational modifications could affect Aβ deposition is unknown. Here, we sought to determine whether extracellular Aβ could be phosphorylated by ecto‐protein kinase in the brain and its pathophysiological implications.
Phosphorylation of extracellular Aβ by ecto‐PKA
To test the phosphorylation of extracellular Aβ, primary cultures of mouse cortical neurons were incubated with synthetic Aβ and [γ‐32P]ATP. Aβ was readily phosphorylated (Figure 1A). No radiolabelling was observed in the absence of cells (data not shown), indicating the presence of an ecto‐protein kinase at the surface of neurons that phosphorylates Aβ (Figure 1A). Aβ was not detected in the corresponding cell lysates, suggesting that Aβ was not internalized and phosphorylated by intracellular kinases under the experimental conditions (Figure 1B). In silico analysis revealed serine residue 8 (Ser8) within a potential recognition motif (R‐x‐x‐S) for PKA (Figure 1C). Phosphorylation of extracellular Aβ was significantly reduced in the presence of the selective PKA inhibitor H‐89, indeed indicating an involvement of an extracellular PKA activity. However, cAMP did not further stimulate Aβ phosphorylation (Figure 1D and E). These findings are consistent with a selective secretion of PKA catalytic subunits and the absence of extracellular regulatory β‐subunits that has been demonstrated previously in tumour cells (Cho et al, 2000b).
To directly demonstrate phosphorylation of Aβ by PKA, different variants of Aβ were incubated with purified PKA in vitro. PKA readily phosphorylated Aβ40 or Aβ42, the two major variants in the human brain. Further in vitro assays using truncated variants, including Aβ1‐16, Aβ17‐40 and pre‐phosphorylated Aβ variants, confirmed the selective phosphorylation of Aβ by PKA at Ser8 (Figure 1F, G and H; Supplementary Figure S1A and B). Phosphorylation of Aβ was detected at low nanomolar concentrations of ATP (Supplementary Figure S1C and D), indicating that physiological concentrations of extracellular ATP in biological fluids allow phosphorylation of Aβ in vivo.
To assess the presence of extracellular PKA activity in the human brain, we performed ex vivo phosphorylation assays with human cerebrospinal fluid (CSF). CSF was incubated with [γ‐32P]ATP and histone, a cognate substrate for PKA. Notably, histone was efficiently phosphorylated by endogenous kinase activity in the CSF. Since this phosphorylation was strongly reduced by H‐89, these data confirm the presence of an extracellular PKA‐like activity in human CSF (Figure 1I). Importantly, human CSF also contained Aβ phosphorylating activity that could be efficiently inhibited by several PKA‐specific inhibitors (KT5720, PKI and H‐89) or by immunodepletion of PKA (Figure 1J). These combined data demonstrate the presence of extracellular PKA in the human brain that could phosphorylate Aβ at Ser8.
Phosphorylation of Aβ promotes its aggregation
We next assessed whether the phosphorylation of Ser8 affects the aggregation of Aβ. Phosphorylated Aβ (pAβ) and non‐phosphorylated Aβ (npAβ) were incubated and aggregation was monitored by Thioflavin T (Figure 2A) or Congo Red assays (Supplementary Figure S2). npAβ showed the expected behaviour in aggregation with a pronounced lag phase before rapid fibril growth in both assays (Figure 2A; Supplementary Figure S2). Notably, pAβ showed significantly increased aggregation as compared with npAβ. However, both variants formed characteristic fibrils highly similar in morphology and size at the end point of this assay (Figure 2B). The analysis of aggregation kinetics revealed that npAβ takes about five times longer to exit the lag phase than pAβ (Supplementary Table SI). It is also interesting to note that, despite the remarkably faster aggregation of pAβ than npAβ, their apparent rate constant values ‘k’ were very similar. Altogether, these data suggest that the higher rate of aggregation for pAβ is predominantly caused by a more efficient nucleation, during which a higher number of small aggregates are formed. To prove increased oligomer formation of pAβ, samples were separated by SDS–PAGE and Aβ variants detected by western blotting (WB). As compared with npAβ, pAβ formed SDS‐resistant low‐molecular weight oligomers (i.e. dimers and trimers) much faster (Figure 2C). Consistent with faster nucleation and oligomer formation, pAβ also showed increased aggregation into larger assemblies that were detected as smears at the upper parts of the gels. These data demonstrate that phosphorylation of Aβ strongly promotes the formation of low‐molecular weight oligomers and further aggregation into fibrils.
The formation of small soluble oligomers is associated with conformational changes resulting in increased β‐sheet structure. We first measured the structural conversion of Aβ monomers to aggregates by circular dichroism (CD) spectroscopy. At the start of the aggregation assay, the initial far‐UV CD spectrum of npAβ revealed the characteristic features of a mostly random coil state (Figure 2D). Incubation of npAβ at 37°C resulted in a prominent change in the CD spectrum. After incubation for 8 h, a CD profile was observed that is characteristic for extended β‐sheet structure (broad negative peak at 210–220 nm; Figure 2D). The initial CD spectrum of pAβ was also as expected for an unfolded peptide. However, a characteristic pattern of extended β‐sheet structure was clearly evident already after 2 h of incubation (Figure 2E), indicating that phosphorylation increases the propensity of Aβ to adopt a β‐sheet conformation and thereby promotes oligomerization. The combined in vitro data demonstrate that phosphorylation of Aβ at Ser8 promotes the formation of oligomers that could increase aggregation.
Next, we compared the effect of pAβ and npAβ variants in nucleation‐dependent polymerization. As expected, preformed oligomeric nuclei of npAβ significantly reduced the lag period of fibril formation as compared with a non‐seeded reaction (Figure 2F). More interestingly, oligomeric nuclei of pAβ were much more efficient in promoting the aggregation reaction of npAβ and almost completely eliminated the lag phase. These data demonstrate that oligomeric nuclei of pAβ could efficiently seed the aggregation of npAβ.
Detection of pAβ in APP transgenic mice and human AD brain
To assess the phosphorylation of Aβ and its effect on aggregation in vivo, we first generated phosphorylation‐state‐specific antibodies. Affinity purified antibody SA5434 was found to be highly specific for Aβ phosphorylated at Ser8 (Supplementary Figure S3A). We also tested several monoclonal antibodies for their binding to pAβ and npAβ. While antibody 82E1 detected both peptides, the antibody 6E10 was found to be highly specific for npAβ (Supplementary Figure S3A). This antibody recognizes an epitope between amino acids 4 and 12 of the Aβ domain that contains the identified phosphorylation site (Kim et al, 1988). Notably, SA5434 did not react with full‐length APP or its C‐terminal fragment in brain extracts of transgenic mice, suggesting selective phosphorylation of Aβ (Supplementary Figure S4). SA5434 showed no reactivity with endogenous mouse APP in non‐transgenic mice, further demonstrating the specificity of this antibody (Supplementary Figure S4). The antibody also did not cross‐react with other modified Aβ species, including synthetic Aβ phosphorylated at Ser26 or pyro Glu‐modified Aβ and with non‐phosphorylated aggregates (Supplementary Figure S3B–D).
We took advantage of the phosphorylation‐state‐specific antibodies to analyse the accumulation and deposition of pAβ and npAβ variants in the brains of APPswe/PS1ΔE9 double transgenic (tg) mice. At an age of 9 months, strong labelling of amyloid deposits with SA5434 was observed in the hippocampal region (Figure 3A). Most deposits also contained npAβ as indicated by the co‐staining with antibody 6E10. In individual plaques, however, a more pronounced reactivity of SA5434 in the core was evident, suggesting preferential deposition of pAβ. Age‐dependent analysis also revealed deposits of pAβ already in the cortex of 2‐month‐old (2m) mice (Figure 3B). Aβ deposition strongly increased with age and a large overlap of staining with antibodies SA5434 and 6E10 was found, indicating co‐deposition of pAβ together with npAβ in extracellular plaques. Again, pAβ appeared to be concentrated in the centre of individual plaques. We also detected small grain‐like deposits selectively labelled by phospho‐specific antibody SA5434 with little if any reactivity for 6E10 (Figure 3B). Such small deposits were not detected in non‐transgenic mice (Supplementary Figure S5). Additional double staining revealed that smaller SA5434 positive deposits of pAβ were not labelled with the fluorescent Congo red analogue K114 (Crystal et al, 2003; LeVine, 2005). In contrast, larger deposits showed a large overlap of SA5434 and K114 labelling (Supplementary Figure S5).
To further demonstrate pAβ in brains of tg mice, we detected pAβ and npAβ by WB. Quantitative analysis revealed that about 20–25% of extracted monomeric Aβ in 18m tg mice is in a phosphorylated state (Supplementary Figure S6). The reactivity of monomeric Aβ with antibody 6E10 markedly increased after treatment of brain homogenates with alkaline phosphatase, also indicating that about 20–30% of monomeric Aβ is in a phosphorylated state in vivo at this age (Supplementary Figure S7). Consistent with the immunohistochemical data, levels of pAβ and npAβ strongly increased with age (Figure 3C). Importantly, SA5434 showed strong reactivity with a smear in the upper regions of the gels, most likely representing oligomeric assemblies of Aβ. These species were already detected at 2 months and became prominent at 6 months. At these ages, SA5434 detected very little monomeric Aβ. In contrast, monoclonal antibody 6E10 readily detected monomeric Aβ already in 6‐months‐old mice that strongly increased with age. As compared with antibody SA5434, the reactivity of antibody 6E10 with oligomeric Aβ assemblies was much weaker and mainly detected in 12‐ and 18‐months‐old animals (Figure 3C). Together, the specific detection of npAβ and pAβ in mouse brain indicates an enrichment of pAβ in oligomeric assemblies and suggests that phosphorylation increased oligomerization of Aβ in vivo.
Deposits containing pAβ were also detected in senile plaques in human AD brain (Figure 4A; Supplementary Figure S8). Strong reactivity with SA5434 was observed in the core of neuritic plaques, while antibody 22C11 against the extracellular domain of APP selectively detected dystrophic neurites in close proximity to the amyloid core of the neuritic plaques (Figure 4B). Importantly, immunoprecipitation of Aβ variants from distinct fractions of human AD brains revealed the selective accumulation of pAβ in SDS‐resistant oligomers (Figure 4C). Antibody SA5434 immunoprecipitated almost exclusively SDS stable dimers from fractions enriched in membrane‐associated/intracellular Aβ (TBS‐TX extracts) or insoluble/fibrillar Aβ (formic acid extracts), while both monomeric and dimeric pAβ were isolated from fractions enriched in soluble extracellular Aβ (TBS extract). In contrast, antibody AW8 detecting both pAβ and npAβ precipitated monomeric and dimeric forms from all three fractions. These data demonstrate that pAβ is highly enriched in oligomeric assemblies of Aβ in the human AD brain and support an increased aggregation of pAβ in vivo. The selective detection of dimeric pAβ upon extraction with formic acid also indicates a very high stability of these toxic assemblies in the human AD brain.
Increased accumulation and toxicity of pseudophosphorylated Aβ in Drosophila
Drosophila expresses an APP‐like protein, but the lack of β‐secretase precludes generation of endogenous Aβ‐like peptides (Bilen and Bonini, 2005). Thus, Drosophila models allow to study the aggregation of exogenous Aβ variants in the absence of endogenous Aβ peptides. First, we tested whether the substitution of Ser8 by an aspartate residue could mimic the aggregation promoting effect of phosphorylation. Importantly, pseudophosphorylated Aβ S8D showed very similar aggregation characteristics like pAβ in vitro and is, therefore, suitable to mimic pAβ (Supplementary Figure S9). To prevent potential effects of the mutation on processing, we generated constructs encoding the Aβ domain with signal sequence that drives selective expression of Aβ variants in the secretory pathway (Crowther et al, 2005). By quantitative real‐time RT–PCR, we identified transgenic lines that express similar mRNA levels (Supplementary Figure S10A).
Consistent with previous results (Finelli et al, 2004; Crowther et al, 2005), expression of Aβ led to age‐dependent degeneration of eyes (Figure 5A). Notably, the pseudophosphorylated Aβ S8D variant led to strongly increased degeneration as compared with Aβ WT (Figure 5A; Supplementary Figure S10B). The degeneration of eyes was associated with significant death of photoreceptor cells (Figure 5B), demonstrating increased toxicity of pseudophosphorylated Aβ. Together, these data indicate that (pseudo)phosphorylated Aβ exerts increased toxicity in vivo. We also detected Aβ levels in corresponding eye extracts. Similar levels of Aβ WT and Aβ S8D were detected in extracts of 1‐day‐old flies, also indicating similar expression of both Aβ variants (Figure 5C). Importantly, pseudophosphorylated Aβ S8D accumulated to much higher levels as compared with Aβ WT in 24‐day‐old flies (Figure 5C), strongly indicating that pseudophosphorylated Aβ S8D showed increased aggregation and accumulation in vivo. Next, we also tested the effects of Aβ WT and Aβ S8D variants upon neuron‐specific expression in the brain on its accumulation as well as on the climbing behaviour of flies, two additional parameters for aggregation and Aβ‐mediated neurotoxicity in vivo (Luheshi et al, 2007). Confocal microscopic analysis of whole‐mount brains revealed age‐dependent accumulation of Aβ peptides. As compared with Aβ WT, Aβ S8D showed highly increased accumulation (Figure 6A). Further, locomotor activity of Aβ S8D flies shows an accelerated decrease compared with Aβ WT or control flies during ageing (Figure 6B; Supplementary Movie S1), indicating a progressive age‐dependent phenotype, which is caused by Aβ peptide accumulation.
Our data demonstrate that the phosphorylation of Aβ increases its aggregation and toxicity. Since this post‐translational modification can occur on WT Aβ, these findings could have very important implications to the pathogenesis of sporadic, late‐onset forms of AD.
Increased aggregation and formation of neurotoxic oligomers of Aβ appears to be critically involved in the initiation and progression of AD (Kayed et al, 2003; Forman et al, 2004; Tsai et al, 2004; Spires et al, 2005; Haass and Selkoe, 2007; Li et al, 2009). This is strongly supported by the identification of mutations within the APP and PS genes that are major causes of EOAD. These mutations commonly cause increased production of Aβ variants that show an increased tendency to form toxic aggregates (Chiti et al, 2003). Especially, the C‐terminal elongated Aβ42 shows strongly increased aggregation as compared with the Aβ40 variant. In addition, several FAD mutations are localized within or close to the β‐turn region of Aβ and could stabilize a β‐sheet conformation of the peptide, thereby also promoting formation of neurotoxic aggregates. Recently, two mutations have been identified in the N‐terminal region of Aβ that also cause EOAD (Hori et al, 2007; Di et al, 2009). This is particularly interesting, because previous studies suggested that this region of the peptide is highly flexible with very little secondary structure and has a minor role in aggregation (Murakami et al, 2002). Moreover, a genetic mutation at position 2 of the Aβ domain was recently identified to cause late‐onset AD in a recessive fashion, indicating that the N‐terminal part of Aβ could have important roles in its aggregation and disease pathogenesis (Di et al, 2009). A critical role of the N‐terminal domain of Aβ is supported by the finding that N‐terminal truncation and formation of pyroglutamate at position 3 of the Aβ peptide also strongly promotes aggregation and is an abundant species found in Aβ deposits in the AD brain (Saido et al, 1995; Schilling et al, 2008). It will now be interesting to assess the relative abundance of the different modified variants of Aβ in human brains or CSF. Mass spectrometry would help to analyse the different modifications of Aβ in vivo and could also provide definite proof whether they occur on individual peptides or probably in combination on the same molecule.
Protein fibrillization generally involves nucleation‐dependent oligomerization as a rate‐limiting step before rapid fibril growth (Rochet and Lansbury, 2000; Chiti et al, 2003). That Aβ plaque formation could be induced by inoculation of amyloid containing brain homogenates into monkeys or APP transgenic mice suggests that nucleation‐dependent fibrillization also occurs in vivo (Walker et al, 2002; Meyer‐Luehmann et al, 2006). The rapid appearance of amyloid plaques within brains of tg mice further supports seeded growth of Aβ fibrils in vivo (Meyer‐Luehmann et al, 2008). Our data demonstrate that phosphorylation at Ser8 promotes the nucleation of Aβ and the formation of oligomers by increasing the propensity to adopt β‐sheet conformation. This might be caused by a relative stabilization of the more extended peptide structure in pAβ, possibly due to its higher negative charge density than the npAβ. Moreover, ordering of disordered regions in the vicinity of the site of phosphorylation might lower the entropic cost for formation of ordered aggregates, contributing to an increased aggregation in case of pAβ when compared with npAβ. Thus, phosphorylated variants of Aβ could trigger oligomerization and deposition of Aβ during pathogenesis of sporadic AD (Figure 7). In agreement with this hypothesis, the nuclei of pAβ were capable to promote aggregation of npAβ in vitro much faster than nuclei of npAβ (Figure 2F).
Importantly, the isolation of small soluble Aβ assemblies from human AD brain strongly supported a critical role of pAβ species in the formation of oligomeric assemblies in vivo. The precipitation with antibodies specifically detecting pAβ revealed a selective enrichment in dimeric Aβ variants, which are known to be highly neurotoxic (Roher et al, 1996; Shankar et al, 2008). In addition, the selective detection of pAβ in dimeric forms could also reflect increased stability of aggregates containing pAβ. Thus, it will be also interesting to assess the stability of aggregates formed by pAβ in comparison with that containing npAβ. The importance of the present finding is further supported by the detection of pAβ in neuritic plaques and SDS‐stable dimers, which strongly argues in favour of a critical role of pAβ in AD‐related neurodegeneration (Roher et al, 1996; Shankar et al, 2008). Furthermore, pAβ also occurs in senile plaques of tg mice and recent studies have shown that such Aβ deposits induce plaque‐associated neuritic degeneration (Tsai et al, 2004; Spires et al, 2005).
Drosophila is a valid model to study aggregation and toxicity of different Aβ variants in vivo. Drosophila melanogaster has been utilized for the understanding of the molecular and cellular basis of AD pathogenesis (Bilen and Bonini, 2005). Aβ expressing fly models offer the possibility of studying Aβ‐induced toxicity and clearance, aggregation propensity and neurodegeneration, genetic and pharmacological screening system for developing therapeutics for AD. Since Drosophila lacks β‐secretase activity, this model allows to assess Aβ‐mediated toxicity in the absence of endogenous Aβ species (Finelli et al, 2004; Bilen and Bonini, 2005; Crowther et al, 2005; Iijima et al, 2008). To mimic pAβ, Ser8 was substituted by an aspartate residue, a strategy that has been successfully used to study the effect of protein phosphorylation (Fluhrer et al, 2004). Importantly, the Aβ S8D variant showed very similar aggregation characteristics like pAβ in vitro, demonstrating the suitability of this strategy. We generated constructs that drive expression of Aβ variants in the secretory pathway without APP, thereby ruling out any effects of the artificial mutation on the production of Aβ peptides by altered proteolytic processing of its precursor. Pseudophosphorylated Aβ S8D strongly promoted age‐dependent degeneration of eyes associated with death of photoreceptor cells as compared with Aβ WT, supporting increased toxicity of pAβ in vivo. Moreover, the selective expression of Aβ variants in brain neurons led to accelerated age‐dependent climbing deficits in flies expressing Aβ S8D, also demonstrating increased toxicity of pAβ species in the brain. Although the exact mode of toxicity is unclear, the combined data demonstrate that mimicking phosphorylation of Aβ strongly promotes age‐dependent dysfunction of brain neurons. It will now be interesting to further dissect the underlying molecular pathways of pAβ‐mediated toxicity and its pathophysiological implications in more detail.
The presence of extracellular protein kinase activities has been widely described, but their biological relevance is poorly understood (Redegeld et al, 1999). These kinases use extracellular ATP that is present in low nanomolar concentrations in the brain, but could increase locally to micromolar concentrations upon certain stimuli, including synaptic activity, inflammation and ischaemia in vivo (Melani et al, 2005; Gourine et al, 2007; Pedata et al, 2007). Our combined data suggest that an extracellular PKA‐like activity is involved in the phosphorylation of Aβ. Interestingly, secretion of PKA has been demonstrated with different peripheral cell types (Cho et al, 2000a). We here show that a PKA‐like activity is also present in the conditioned media and/or the cell surface of primary mouse cortical neurons. Moreover, we identified a PKA‐like activity in human CSF. These novel data strongly indicate that secretion of PKA occurs in the human brain. However, further studies are required to identify the molecular mechanism that underlie and regulate PKA secretion in the brain.
Because our data also indicate an important role of extracellular or cell surface‐localized protein kinases in the pathogenesis of AD, these kinases could represent potential targets to decrease Aβ aggregation and toxicity. Further, the detection of phosphorylated and npAβ in biological fluids could also be explored for evaluation as biomarkers.
Materials and methods
Chemicals and antibodies
The following chemicals and antibodies were used: adenosine‐3′, 5′‐cyclic monophosphate (Biolog Life Science Institute, Germany), Congo red (AppliChem GmbH, Germany), K114, a fluorescent amyloid‐specific dye (an analogue of Congo Red), Thioflavin T, Histone, H‐89, PKI and KT5720 were purchased from Sigma (Germany) and Aβ peptides were from Peptide Specialty Laboratory (Heidelberg, Germany). Monoclonal Aβ antibodies 6E10 and 4G8 were purchased from Signet Laboratories, 82E1 antibody was from IBL Corporation (Japan), 2G3 and 21F12 antibodies, which specifically recognize Aβ terminating at residues 40 and 42, were generous gift from Drs Peter Seubert and Dale Schenk (Elan Pharmaceuticals). AW8, a polyclonal anti‐Aβ antibody, was raised to aggregated synthetic Aβ1‐42 and fluorochrome‐coupled anti‐mouse IR800 antibody was from Rockland (Gilbertsville, PA). Monoclonal APP antibody 22C11 was purchased from Chemicon. The Cy‐2 and Cy‐3 fluorochromes were from Dianova (Germany) and Alexa Fluor 488 goat anti‐mouse and Alexa Fluor 594 goat anti‐rabbit secondary antibodies were purchased from Invitrogen (Germany). The PKA Cα‐ and Cβ‐specific antibodies were from Santa Cruz biotechnology.
Generation of phosphorylation‐state‐specific antibodies
The polyclonal phospho‐specific antibody SA5434 was generated in rabbits by inoculation of synthetic peptide that represents amino acids 1–16 of Aβ with a phosphoserine at position 8 coupled to keyhole limpet haemocyan (Eurogentec, Belgium). Phosphorylation‐state‐specific antibodies were isolated from the serum by double affinity purification against the phosphorylated and non‐phosphorylated peptide. The specificity of the antibodies was characterized by ELISA and WB.
In vitro, in vivo and ex vivo phosphorylation of Aβ using purified PKA, primary mouse neurons and human CSF are described in detail in Supplementary data.
Aβ aggregation assays
Aβ aggregation assays were carried out using synthetic npAβ and pAβ peptides and monitored by Thioflavin T, Congo Red, CD Spectroscopy and transmission electron microscopy. Further information is described in Supplementary data.
Transgenic mice, protein extraction and immunohistochemistry
The biochemical and immunohistochemical analyses of transgenic mice were carried out using APPswe/PS1ΔE9 double transgenic mice (Strain Name: B6C3‐Tg, Jax Laboratories). Preparation of brain homogenates, protein extraction, WB, quantification and immunohistochemistry are described in detail in Supplementary data.
Analysis of human AD brain
Human AD brains were obtained from the University Hospital Bonn with the laws and the permission of the local ethical committees. Immunofluorescence and confocal microscopy of human AD brain, human brain sample preparation and quantitation of Aβ in human brain extracts by immunoprecipitation and western‐blotting analysis are described in details in Supplementary data.
In vitro seeding assay
In vitro seeded aggregation of npAβ was carried out using preaggregated npAβ and pAβ as seeds and aggregation was followed by ThT fluorescence measurement. The detailed procedures of all the above are described in Supplementary data.
Supplementary data are available at The EMBO Journal Online (http://www.embojournal.org).
Conflict of Interest
The authors declare that they have no conflict of interest.
Supplementary Video 1
We thank P Wunderlich for protein quantifications by ECL imaging, Dr C Barry for help with the in vitro aggregation assays, Dr D Riedel for the electron micrographs, and Dr D Crowther for providing pUAST Aβ42 and Aβ40 plasmid vectors. This work was supported by the Deutsche Forschungsgemeinschaft (DFG) to JW (WA1477/6, SFB645, KFo177) and to MZ by the DFG and the Max Planck Society.
Author Contributions: SK wrote parts of the paper and performed most of the experiments. DT, DRT and MTH contributed in immunohistochemical analyses and discussions; NR‐G and MZ performed CD spectroscopy and seeding experiments, and contributed in data analyses and discussions. MR and MH contributed in the generation and analysis of transgenic Drosophila; DMW and JMM analysed pAβ in human AD brain extracts. JW conceived and supervised the research project, and wrote main parts of the manuscript. All authors were involved in the design of experiments and data interpretation.
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