A PACS‐1, GGA3 and CK2 complex regulates CI‐MPR trafficking

Gregory K Scott, Hao Fei, Laurel Thomas, Guruprasad R Medigeshi, Gary Thomas

Author Affiliations

  1. Gregory K Scott,
  2. Hao Fei,
  3. Laurel Thomas,
  4. Guruprasad R Medigeshi and
  5. Gary Thomas*,1
  1. 1 Vollum Institute, Portland, OR, USA
  1. *Corresponding author. Vollum Institute, Oregon Health & Science University, Oregon Health Sciences University, L‐474, 3181 SW Sam Jackson Park Road, Portland, OR 97239‐3098, 97239, USA. Tel.: +1 503 494 6955; Fax: +1 503 494 1218; E-mail: thomasg{at}
  • Present address: Department of Psychiatry and Biobehavioral Sciences, UCLA, Los Angeles, CA 90095, USA

  • Present address: Department of Molecular Microbiology and Immunology, OHSU, Portland, OR, USA

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The cation‐independent mannose‐6‐phosphate receptor (CI‐MPR) follows a highly regulated sorting itinerary to deliver hydrolases from the trans‐Golgi network (TGN) to lysosomes. Cycling of CI‐MPR between the TGN and early endosomes is mediated by GGA3, which directs TGN export, and PACS‐1, which directs endosome‐to‐TGN retrieval. Despite executing opposing sorting steps, GGA3 and PACS‐1 bind to an overlapping CI‐MPR trafficking motif and their sorting activity is controlled by the CK2 phosphorylation of their respective autoregulatory domains. However, how CK2 coordinates these opposing roles is unknown. We report a CK2‐activated phosphorylation cascade controlling PACS‐1‐ and GGA3‐mediated CI‐MPR sorting. PACS‐1 links GGA3 to CK2, forming a multimeric complex required for CI‐MPR sorting. PACS‐1‐bound CK2 stimulates GGA3 phosphorylation, releasing GGA3 from CI‐MPR and early endosomes. Bound CK2 also phosphorylates PACS‐1Ser278, promoting binding of PACS‐1 to CI‐MPR to retrieve the receptor to the TGN. Our results identify a CK2‐controlled cascade regulating hydrolase trafficking and sorting of itinerant proteins in the TGN/endosomal system.

  • Received January 10, 2006.
  • Accepted August 16, 2006.
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