Human angiotensin‐converting enzyme 2 (ACE2) is a functional receptor for SARS coronavirus (SARS‐CoV). Here we identify the SARS‐CoV spike (S)‐protein‐binding site on ACE2. We also compare S proteins of SARS‐CoV isolated during the 2002–2003 SARS outbreak and during the much less severe 2003–2004 outbreak, and from palm civets, a possible source of SARS‐CoV found in humans. All three S proteins bound to and utilized palm‐civet ACE2 efficiently, but the latter two S proteins utilized human ACE2 markedly less efficiently than did the S protein obtained during the earlier human outbreak. The lower affinity of these S proteins could be complemented by altering specific residues within the S‐protein‐binding site of human ACE2 to those of civet ACE2, or by altering S‐protein residues 479 and 487 to residues conserved during the 2002–2003 outbreak. Collectively, these data describe molecular interactions important to the adaptation of SARS‐CoV to human cells, and provide insight into the severity of the 2002–2003 SARS epidemic.
SARS coronavirus (SARS‐CoV) is the etiological agent of severe acute respiratory syndrome (SARS), an acute pulmonary syndrome that, when it emerged in the winter of 2002–2003, resulted in the death of approximately 800 individuals, close to 10% of those infected (Drosten et al, 2003; Fouchier et al, 2003; Ksiazek et al, 2003; Kuiken et al, 2003). Despite concerns that SARS‐CoV would re‐emerge, last winter (2003–2004), only a handful of individuals were found infected by the virus. These individuals appeared to have much less severe symptoms, and no secondary transmission was observed (Liang et al, 2004; Peiris et al, 2004; Song et al, 2005). Severe cases of SARS were also reported in 2004, but these resulted from laboratory infections (Normile, 2004).
The coronavirus spike (S) protein mediates infection of receptor‐bearing cells (Gallagher and Buchmeier, 2001; Holmes, 2003; Hofmann and Pohlmann, 2004). Angiotensin‐converting enzyme 2 (ACE2) is a functional receptor for SARS‐CoV, and binds the SARS‐CoV S protein with high affinity (Li et al, 2003). Several lines of evidence suggest that ACE2 is a physiologically relevant receptor during infection. Tissue expression of the receptor corresponds to the localization of virus in infected individuals and animals (Harmer et al, 2002; Chan et al, 2004; Ding et al, 2004; Hamming et al, 2004). Also, the efficiency of infection in humans, mice, and rats correlates with the ability of the ACE2 of each species to support viral replication (Li et al, 2004; Subbarao et al, 2004; Wentworth et al, 2004). Antibodies that block ACE2 association (Sui et al, 2004) protect mice against infection (Sui et al, 2005). Finally, although many cell lines do not express ACE2, most cell lines shown to support SARS‐CoV infection or replication detectably express this receptor (Hofmann et al, 2004; Nie et al, 2004). Although DC‐SIGNR (L‐SIGN, CD209L) and DC‐SIGN (CD209) have recently been shown to enhance infection of ACE2‐expressing cells (Jeffers et al, 2004; Marzi et al, 2004; Yang et al, 2004), these proteins do not appear to mediate efficient infection in the absence of ACE2 (Jeffers et al, 2004; Marzi et al, 2004).
Unlike many type I fusion proteins, including those of other coronaviruses, the S protein of SARS‐CoV is not cleaved in the virus‐producing cell (Xiao et al, 2003; Moore et al, 2004). However, two domains corresponding to the S1 and S2 proteins of processed coronaviruses can be defined (Gallagher and Buchmeier, 2001). The S1 domain mediates receptor association, whereas the S2 domain is membrane‐associated and likely undergoes structural rearrangements that mediate membrane fusion. A discrete receptor‐binding domain (RBD) of the S protein has been defined at residues 318–510 of the S1 domain (Xiao et al, 2003; Babcock et al, 2004; Wong et al, 2004). This RBD binds ACE2 with higher affinity than does the full S1 domain (Wong et al, 2004).
Early cases of SARS in 2002 were reported to have occurred in animal traders and restaurant workers handling wild mammals, and SARS‐CoV has been isolated from two such mammals, palm civets (Paguma larvata) and raccoon dogs (Nyctereutes procyonoides) (Guan et al, 2003). Palm civets have also been implicated in the minor 2003–2004 SARS outbreak (Zhong, 2004). The sequences of the S‐protein genes of 13 viruses isolated from palm civets, and one from raccoon dogs, have been determined (Guan et al, 2003; Song et al, 2005). Interestingly, the RBDs of these animal S proteins are highly conserved except residue 479, which varies between asparagine and basic amino acids, but differ at several positions from the RBDs of viruses isolated during the 2002–2003 outbreak. Although different from palm‐civet‐derived RBDs, the latter RBDs were themselves highly conserved in the more than 100 S‐protein genes obtained during the severe human outbreak (He et al, 2004; Zhang et al, 2004). Full sequence has also been published for two S‐protein genes obtained during the mild 2003–2004 outbreak (He et al, 2004; Song et al, 2005). These genes are nearly identical and contain elements common to S protein isolated during the earlier human outbreak and to that isolated from palm civets.
Here we describe the S‐protein‐binding domain of human ACE2 by characterizing chimeras of human and rat ACE2. By introducing four residues of human ACE2 into rat ACE2, we convert this receptor, which supports little or no S‐protein‐mediated infection, into a receptor that binds the S1 domain and supports infection, with efficiency close to that of human ACE2. By characterizing additional ACE2 variants, we localize the S‐protein‐binding domain primarily to α‐helix 1 of ACE2 and to a loop leading to β‐sheet 5. We then show that a representative S protein from the mild 2003–2004 outbreak, and one from palm civets, mediates more efficient infection of cells expressing palm‐civet ACE2 compared to cells expressing human receptor. In contrast, S protein from the severe 2002–2003 outbreak efficiently binds and utilizes both receptors. Two regions of the S‐protein‐binding site on ACE2, and two residues in the RBD of these S proteins, largely determine these differences. Our data describe S‐protein adaptations, and their receptor counterparts, that permit efficient infection of human cells. These adaptations, absolutely conserved during 2002–2003 outbreak, may in part account for the unusual severity of SARS.
Localization of the S‐protein‐binding domain on human ACE2
We have previously shown that S‐protein‐mediated infection of cells expressing human ACE2 is substantially more efficient than that of cells expressing the same levels of rat ACE2 (Li et al, 2004). We first investigated whether this difference localized to the ACE2 enzymatic or collectrin domain. The latter domain is defined by its close homology with a small, kidney‐expressed protein of the same name (Zhang et al, 2001). Figure 1A shows that a fusion protein comprising the SARS‐CoV S1 domain and the Fc domain of human IgG1 (S1‐Ig) efficiently precipitated human ACE2, as well as an ACE2 chimera with the human catalytic domain and the rat collectrin domain. In contrast, S1‐Ig could not precipitate rat ACE2 or an ACE2 chimera with the rat catalytic domain and the human collectrin domain. The ability of these chimeric receptors to bind S1‐Ig was also reflected in their ability to support S‐protein‐mediated infection (Figure 2C). These data indicate that differences in the ability of rat and human ACE2 to support infection are localized to the catalytic domain of the receptor.
Guided by the structure of the ACE2 catalytic domain (Towler et al, 2004), we made a series of human ACE2 variants in which one or a few solvent‐exposed residues were altered to their rat ACE2 counterparts. Two variants consistently bound S1‐Ig less efficiently than did wild‐type human ACE2. Introduction of rat residues 82–84 (NFS), which include a glycosylation site at asparagine 82 not present in human ACE2, partially inhibited S1‐Ig association (Figures 1B and 2A) and S‐protein‐mediated entry (Figure 2C). Alteration of lysine 353 to a histidine residue present on the rat receptor interfered more dramatically with S1‐Ig association, and also partially inhibited S‐protein‐mediated entry. Introduction of human residues 82–84 (MYP) and lysine 353 into rat ACE2 resulted in substantial increases in S1‐Ig binding, as assayed both by immunoprecipitation and by flow cytometry (Figure 2A and B). Combination of both sets of residues resulted in a rat ACE2 variant that bound S1‐Ig and supported S‐protein‐mediated infection comparably to human ACE2 (Figure 2A–C). These data suggest that residues 82–84 and, more so, lysine 353 participate in S‐protein association with human ACE2.
We subsequently altered a number of additional residues of human ACE2 in the vicinity of residues 82–84 and 353 to alanine or, in some cases, aspartic acid. Alteration of two residues on the first helix of ACE2, at lysine 31 and tyrosine 41, substantially interfered with the S1‐Ig association, as did residues adjacent to lysine 353, at aspartic acid 355 and at arginine 357, both within ACE2 β‐sheet 5 (Figure 3A). Figure 3B–D shows three views of the crystal structure of human ACE2, in which residues that convert rat ACE2 to an efficient SARS‐CoV receptor are shown in red, and additional residues whose alteration interferes with S1‐Ig association are shown in yellow. Green indicates residues whose alteration did not affect S1‐Ig binding. The C‐terminal collectrin domain is not well ordered in the structure, but is used here to position the molecule with respect to the cell membrane. As shown in the figures, the S‐protein‐binding site on ACE2 is localized above the deep cleft that harbors the catalytic site and on the upper left of the structure when viewed facing that cleft.
S‐protein association is independent of ACE2 conformational changes
The crystal structure of ACE2 has been solved in two distinct conformations, an open one (Figure 3B–D) and a conformation in which the cleft is closed around the ACE2 inhibitor MLN‐4760 (Dales et al, 2002; Towler et al, 2004). These two conformations likely reflect free and substrate‐bound states of the enzyme, respectively. We investigated whether the large conformational change associated with inhibitor binding interfered with S1‐Ig binding or S‐protein‐mediated infection. As previously reported, MLN‐4760 inhibits ACE2 activity in the low nanomolar range (Dales et al, 2002), and no ACE2 enzymatic activity was detected in the presence of 100 nM inhibitor (Figure 4A). However, 100 nM MLN‐4760 did not interfere with immunoprecipitation of ACE2 by S1‐Ig, nor did this inhibitor interfere with S‐protein‐mediated infection (Figure 4B and C). Consistent with these observations, the distances among α and β carbons of residues 31, 41, 353, 355, and 357 in the S‐protein‐binding site of ACE2 varied by less than 0.4 Å between the inhibitor‐bound and ‐unbound structures (Towler et al, 2004). In contrast, distances between these residues and residues across the cleft typically varied by greater than 6 Å in the two structures. These data suggest that the S‐protein‐binding region of ACE2 is not perturbed by inhibitors or substrates that induce large conformational changes in the receptor. Consistent with these studies, we have also observed, using the assay shown in Figure 4A, that S1‐Ig does not interfere with the enzymatic activity of ACE2 (data not shown).
Association of three S proteins with human and palm‐civet ACE2
The S protein used in the studies above was obtained from a patient infected during the severe 2002–2003 SARS‐CoV outbreak. Another outbreak, during the winter of 2003–2004, caused much less severe symptoms in the few individuals infected and resulted in no documented human‐to‐human transmissions (He et al, 2004; Liang et al, 2004; Zhong, 2004; Song et al, 2005). We compared an S protein of virus isolated during this latter outbreak (GD03T0013; accession number AY525636; denoted GD herein) with that of virus obtained during the 2002–2003 outbreak (TOR2; AY274119) and with that isolated from palm civets (SZ3; AY304486) (Guan et al, 2003; Marra et al, 2003; Rota et al, 2003; He et al, 2004; Song et al, 2005). Figure 5A shows that the S1 domains of all three S proteins efficiently bound palm‐civet ACE2 (accession number AY881174), whereas only the S1 domain of TOR2 efficiently bound human ACE2. Of note, the S1 domain of virus isolated during the 2003–2004 outbreak bound palm‐civet ACE2 much more efficiently than it bound human ACE2. We then investigated the RBD of each of these S‐protein variants. The ability of these S1 domains to bind palm‐civet and human ACE2 was reflected in the ability of their respective RBDs to bind these receptors (Figure 5B). We also assayed the ability of the entire S protein to mediate infection of cells expressing human or palm‐civet ACE2. The efficiency of entry was consistent with the ability of the S1 domain of each variant to bind each ACE2 (Figure 6C). These data are consistent with the hypothesis that the palm civet is a source of SARS‐CoV, and suggest that the apparent lack of severity of disease during the 2003–2004 outbreak may be due in part to incomplete adaptation of GD virus to human ACE2.
S‐protein RBD determinants of association with human ACE2
The RBDs of TOR2 and SZ3 differ by four residues (Figure 6A). We investigated which of these residues contribute to the ability of TOR2 RBD to bind efficiently human ACE2. Each residue in the TOR2 RBD was altered to its SZ3 counterpart. Alteration of two residues, at positions 479 and 487, interfered with the association of the TOR2 RBD with human ACE2 (Figure 6B). Surface plasmon resonance studies further demonstrated a greater than 20‐fold decrease of affinity for human ACE2 when either residue 479 or 487, but not when residue 344 or 360, is altered to its palm‐civet counterpart (Table I and Supplementary Figure 2). Notably, alteration of threonine 487 to serine also affected the ability of the TOR2 RBD to associate with palm‐civet ACE2 (Figure 6B). Introduction of TOR2 residues at 479 or 487 substantially increased the ability of the full SZ3 S protein to infect cells expressing human ACE2. Introduction of SZ3 residues at these positions into the TOR2 S protein resulted in a 2‐ to 3‐fold decrease in infection of these cells (Figure 6C). These data suggest that adaptation of S protein to human ACE2 is facilitated by alteration of residue 479 to asparagine and of 487 to threonine.
Palm‐civet and human ACE2 determinants of differential S‐protein association
We also investigated determinants on civet ACE2 that participate in its ability to facilitate efficient infection by GD and SZ3. Two regions within the S‐protein‐binding site differed significantly between human and palm‐civet ACE2 (see Figure 7D and E). A region of α‐helix 1 (residues 30–40) varied at six residues between human and civet ACE2. Likewise, a loop initiating ACE2 α‐helix 3 differed by four residues (90–93). A glycosylation site at asparagine 90 of human ACE2 is part of this latter region and is not present in palm‐civet ACE2. Figure 7A shows that the S1 domains of GD and SZ3 bound substantially more efficiently when either of these regions was introduced into human ACE2, and that introduction of both regions resulted in receptor binding comparable to that with wild‐type palm‐civet ACE2. These observations were also reproduced in infection assays (Figure 7B). These data demonstrate that residues within the S‐protein‐binding domain of ACE2 largely determine the efficiency with which GD and SZ3 S proteins bind and utilize human and palm‐civet ACE2.
Binding of RBD chimeras to ACE2 chimeras
Figure 7C compares the ability of eight RBD variants to bind to human and civet ACE2, as well as to the chimeric molecules assayed in Figure 7A and to a point‐mutation variant of palm‐civet ACE2, in which aspartic acid 354 was altered to a glycine present in human ACE2. RBD variants were generated from that of TOR2 (left panels) or SZ3 (right panels), and altered at positions 479, 487, or both, as indicated. The panels of Figure 7C permit several conclusions. First, the rough equivalence between the left and right panels indicates that, as implied by Figure 6B and C, S‐protein residues 479 and 487 account for most of the differences between TOR2 and SZ3 RBD. Second, no consistent differences were observed between palm‐civet ACE2 and its variant with glycine at residue 354, indicating little or no contribution of this residue to S‐protein association. Third, consistent with infection data in Figure 6C, the presence of threonine at residue 487 enhanced the affinity of most RBDs for civet and human ACE2, and for variants of these receptors. (Compare, for example, K479/S487 RBD variants with K479/T487 variants for their ability to precipitate each ACE2 variant.) Fourth, and in contrast, substitution of lysine 479 for asparagine in most contexts increased the ability of each RBD variant to associate with human, but not with palm‐civet, ACE2. (Compare the binding of N479/T487 RBD with that of K479/T487 variants for binding to human ACE2 (lane 1) and palm‐civet ACE2 (lane 6); likewise for N479/S487 and K479/S487 variants.) This enhancement was also observed for ACE2 chimeras containing human α‐helix 1 residues (lanes 1 and 2), but not those of palm civet (lanes 3, 5, and 6), whereas residues 90–93 did not determine sensitivity to RBD residue 479. Fifth, consistent with infection data in Figure 7B and Supplementary Figure 3, all RBDs bound ACE2 variants bearing residues 90–93 of palm‐civet ACE2 substantially more efficiently than they bound equivalent variants with human ACE2 residues at these positions (compare lanes 1 and 2 and lanes 3 and 4 in each panel). Thus, the data of Figure 7C indicate that a lysine at S‐protein residue 479 interferes with RBD association with human, but not palm‐civet, ACE2. Supplementary Figure 4 shows data consistent with a steric interaction between lysine 31 of human ACE2 and lysine 479 of the SZ3 S protein. Our data also show that alteration of S‐protein serine 487 to threonine increases RBD affinity for both human and civet ACE2. Finally, they suggest that no S protein studied has fully adapted to human ACE2 residues 90–93, consistent with a recent zoonotic transmission of the virus.
Mapping determinants to the ACE2 surface
Figure 7D–F summarizes our findings. Figure 7D shows amino‐acid sequences of regions critical to S‐protein association for palm‐civet, rat, and human ACE2. Figure 7E shows ACE2 oriented with the C‐terminal membrane‐associated collectrin domain facing away from the viewer. Red indicates residues whose alteration transformed rat ACE2 to an efficient SARS‐CoV receptor. Orange indicates additional residues common to rat and human ACE2 whose alteration also interferes with S‐protein association. Yellow indicates residues along the α‐helix 1 ridge that are unique to palm‐civet ACE2, and which permit efficient association with RBD isolates from palm civet and likely interact with lysine 479 of the palm‐civet RBD. K31 of human ACE2, which interferes with palm‐civet RBD lysine 479, is labeled with white text in Figure 7E. Four residues at the beginning of α‐helix 4 that permit more efficient binding and infection by all S proteins assayed are shown in cyan, and the glycosylation site in this region, present in human but not palm‐civet ACE2, is shown in green.
ACE2 is a functional receptor for SARS‐CoV, and is likely to play a critical role in viral replication in an infected host (Li et al, 2003; Hamming et al, 2004; Nie et al, 2004). Here we describe the S‐protein‐binding domain of ACE2. In particular, residues along the first α‐helix, and lysine 353 and proximal residues at the N‐terminus of β‐sheet 5, participate in S‐protein binding and in infection. By altering histidine 353 in rat ACE2 and modifying a glyosylation site that may alter the shape of α‐helix 1, we converted rat ACE2 to an efficient receptor for SARS‐CoV. This S‐protein‐binding region of ACE2 remains intact in the presence of an inhibitor that dramatically alters the overall conformation of ACE2 (Dales et al, 2002; Towler et al, 2004), consistent with the inability of this inhibitor to block infection, and with the inability of the S protein to modulate ACE2 activity.
Although there can be multiple constraints on interspecies transmission of viruses (Webby et al, 2004), S‐protein alterations are sufficient to extend or alter the host range of a number of coronaviruses (Kuo et al, 2000; Casais et al, 2003; Haijema et al, 2003; Schickli et al, 2004). We have shown that entry is the primary barrier to SARS‐CoV infection of murine cells (Li et al, 2004). These observations suggest that S‐protein changes may be critical to or sufficient for the adaptation of SARS‐CoV to human cells. Accordingly, we compared the S proteins derived from the 2002–2003 outbreak (TOR2), from the less severe 2003–2004 outbreak (GD), and from apparently healthy palm civets (SZ3) (Guan et al, 2003; He et al, 2004). Strikingly, the receptor‐binding regions of each of these S proteins bound palm‐civet ACE2 efficiently, but only that from the 2002–2003 outbreak bound human ACE2 with comparable efficiency. These data are consistent with the absence of human‐to‐human transmission during the 2003–2004 outbreak, and with recent transmission of SARS‐CoV from palm civets to humans (Guan et al, 2003; Zhong, 2004; Song et al, 2005).
Differences among these S proteins permitted identification of key changes necessary for adaptation to the human receptor. In particular, changes at S‐protein residues 479 and 487 appear to be critical for high‐affinity association with human ACE2. The alteration at 479 to a small, uncharged residue is a consistent property of all described SARS‐CoV obtained from humans, whereas most civet‐derived viruses retain a basic residue at this position (Guan et al, 2003; Marra et al, 2003; Rota et al, 2003; He et al, 2004; Zhang et al, 2004; Song et al, 2005). Our data indicate that residue 479 interacts with residues along a ridge formed by ACE2 α‐helix 1, and in particular with lysine 31, which is present in human but not palm‐civet ACE2. Alteration of S‐protein residue 479 to the asparagine found in virus isolated from humans appears to accommodate this human ACE2 lysine.
Differences at S‐protein residue 487 are also of interest. A threonine at position 487 is absolutely conserved in all of the more than 100 S proteins isolated during the severe 2002–2003 outbreak. In contrast, the S proteins of viruses isolated during the 2003–2004 outbreak, and all 14 animal SARS‐CoV isolated, had a serine at this position (Guan et al, 2003; He et al, 2004; Zhang et al, 2004; Song et al, 2005). A threonine at position 487 increased affinity of most RBDs assayed for both human and palm‐civet ACE2, and all chimeras thereof, and substantially enhanced the efficiency with which palm‐civet‐derived S protein infected cells expressing human ACE2. These observations indicate that the additional methyl group of threonine 487 participates in the efficiency of infection of human and non‐human cells.
S‐protein alterations at residues 479 and 487 are important for high‐affinity association with human ACE2, and for efficient infection of cells expressing this receptor. Knowledge of these residues may be useful in assessing the risk posed by any new SARS‐CoV outbreak. Our data also show that, even with these and other changes outside the RBD, SARS‐CoV is imperfectly adapted to its human receptor. In particular, introduction of residues 90–93 of civet ACE2 into the human receptor increased binding of, and infection mediated by, all S proteins assayed. This effect may be due to removal of a glycosylation site at position 90 to which no SARS‐CoV has fully adapted. This observation raises the possibility that soluble human ACE2 lacking this glycosylation would more effectively inhibit SARS‐CoV replication than wild‐type human ACE2.
We have previously shown that replication of SARS‐CoV in a murine cell line is limited by the low affinity of the S protein for murine ACE2 (Li et al, 2004). Moreover, the affinity of S protein for the receptors of rats, mice, and humans correlates with the ability of virus to replicate in these animals. The lower affinity of palm‐civet‐derived S protein for the palm‐civet receptor is consistent with this pattern in that no overt disease was manifest in animals from which this virus was isolated (Guan et al, 2003), but disease was observed in palm civets challenged with isolates obtained during the 2002–2003 outbreak (Wu et al, 2005). Together, these observations suggest that the affinity of S protein for ACE2 is an important determinant in the overall rate of viral replication and in the severity of disease. If so, adaptations within the S protein that are critical for high‐affinity association with human ACE2 may have contributed to the unusual severity of SARS.
Materials and methods
Construction of S‐protein and ACE2 variants
Plasmid encoding a codon‐optimized form of the SARS‐CoV S protein of the TOR2 isolate (accession number AY274119) has been previously described (Li et al, 2003; Moore et al, 2004). Plasmids encoding the corresponding S proteins of the GD03T0013 isolate, isolated during the mild 2003–2004 outbreak (accession number AY525636; denoted GD herein), and the SZ3 isolate, isolated from palm civets (accession number AY304486), were generated de novo by recursive PCR. Plasmids encoding the S1 domain (residues 12–672) and the RBD (residues 318–510) of the TOR2 S protein, fused to the Fc domain of human IgG1 (S1‐Ig and RBD‐Ig, respectively), have been previously described (Li et al, 2003; Wong et al, 2004). Corresponding S1‐Ig and RBD‐Ig variants of the GD and SZ3 isolates and variant ACE2 molecules were generated by mutagenesis using the QuikChange method (Invitrogen). Human, rat, and palm‐civet ACE2 molecules were amplified from cDNA of corresponding tissue by PCR, and cloned into a vector encoding previously described amino‐ and carboxy‐terminal tags (Li et al, 2004).
Association of S1‐Ig or RBD‐Ig with ACE2 variants was determined by flow cytometry and by immunoprecipitation. Flow cytometry using ACE2‐expressing cells has been previously described (Li et al, 2003; Wong et al, 2004). Briefly, HEK293T cells were transfected with a plasmid encoding ACE2 variants, or with vector alone. At 2 days post‐transfection, cells were detached in PBS/5 mM EDTA and washed with PBS/0.5% BSA. S1‐Ig or RBD‐Ig, or variants thereof, or the anti‐tag antibody 9E10, were added to 106 cells, and the mixture was incubated on ice for 1 h. Cells were washed three times with PBS/0.5% BSA, and then incubated for 30 min on ice with anti‐human IgG FITC conjugate (Sigma). Cells were again washed with PBS/0.5% BSA, and analyzed.
Immunoprecipitations were performed as previously described (Li et al, 2003; Wong et al, 2004). Briefly, HEK293T cells were transfected with plasmid encoding ACE2 variants and radiolabeled with [35S]cysteine and [35S]methionine. After 2 days, transfected cells were harvested and lysed in PBS buffer containing 1% CHAPSO. Cell lysates were incubated with Protein A–Sepharose beads together with 2 μg S1‐Ig or RBD‐Ig variants, or with the antibodies 1D4, recognizing a carboxy‐terminal C9 tag on ACE2, or 9E10, recognizing an amino‐terminal myc tag. Protein A–Sepharose beads were washed three times in PBS/0.5% CHAPSO, and analyzed by SDS–PAGE. Immunoprecipitated ACE2 variants were quantified by phosphorimaging.
TOR2 RBD‐Ig variants were also assayed by surface plasmon resonance using a Biacore 3000. A 200 nM portion of purified RBD‐Ig of TOR2 variants was bound to an anti‐human antibody (Sigma I‐2136) immobilized on a CM5 sensor chip. Soluble human ACE2 in HBS‐EP buffer (Biacore) was introduced at a flow rate of 20 μl/min at concentrations of 700, 200, 40, 8, 1.6, and 0 nM. Kinetic parameters were determined with BIA‐EVALUATION software (Biacore).
Infection with S‐protein‐pseudotyped retrovirus
MLV expressing GFP and pseudotyped with SARS‐CoV S‐protein variants has been previously described (Moore et al, 2004). Briefly, MLV virions were generated by cotransfecting plasmid encoding MLV gag and pol genes, the pQCXIX vector (BD Sciences) expressing GFP, and plasmid encoding S‐protein variants. At 48 h post‐transfection, cell supernatants were normalized for reverse transcriptase activity and incubated with HEK293T cells transfected with ACE2 variants. At 48 h postincubation, GFP fluorescence of infected cells was measured by flow cytometry. In some cases, cells were preincubated for 1 h with the ACE2 inhibitor MLN‐4760 or with NH4Cl before infection, and equivalent concentrations were maintained during infection.
Infection was also assayed with a lentivirus expressing a luciferase reporter gene and pseudotyped with S‐protein variants, as previously described (Sui et al, 2005). Briefly, 293T cells were cotransfected with plasmid encoding S‐protein variants, a plasmid (pCMVΔR8.2) encoding HIV‐1 Gag‐Pol, and a plasmid (pHIV‐Luc) encoding the firefly luciferase reporter gene under control of the HIV‐1 long terminal repeat. At 2 days post‐transfection, viral supernatants were harvested and 3 ìl of S‐protein‐pseudotyped virus was used for infection of 6000 ACE2‐expressing 293T cells in a 96‐well plate. Infection efficiency was quantitated by measuring the luciferase activity in the target cells with an EG&G Berthold Microplate Luminometer LB 96V.
ACE2 enzymatic activity
The enzymatic activity of ACE2 was assayed using a fluorogenic substrate, 7‐methoxycoumarin‐YVADAPK(2,4‐dinitrophenyl)‐OH (R&D Systems). Cleavage of this peptide by ACE2 removes the 2,4‐dinitrophenyl moiety that quenches the fluorescence of the 7‐methoxycoumarin moiety. A 1 μg portion of a soluble form of ACE2 (Moore et al, 2004) was incubated in 100 mM Tris buffer with varying concentrations of the ACE2 inhibitor MLN‐4760 (Dales et al, 2002). Fluorescence was monitored at 5 min intervals using an excitation wavelength of 330 nm and emission wavelength of 450 nm.
Supplementary data are available at The EMBO Journal Online.
Supplementary Figure 1
Supplementary Figure 2
Supplementary Figure 3
Supplementary Figure 4
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