Tissue plasminogen activator (tPA) is a serine protease involved in the degradation of blood clots through the activation of plasminogen to plasmin. Here we report on the identification of tPA as a specific protease able to activate platelet‐derived growth factor C (PDGF‐C). The newly identified PDGF‐C is secreted as a latent dimeric factor (PDGF‐CC) that upon proteolytic removal of the N‐terminal CUB domains becomes a PDGF receptor α agonist. The CUB domains in PDGF‐CC directly interact with tPA, and fibroblasts from tPA‐deficient mice fail to activate latent PDGF‐CC. We further demonstrate that growth of primary fibroblasts in culture is dependent on a tPA‐mediated cleavage of latent PDGF‐CC, generating a growth stimulatory loop. Immunohistochemical analysis showed similar expression patterns of PDGF‐C and tPA in developing mouse embryos and in tumors, indicating both autocrine and paracrine modes of activation of PDGF receptor‐mediated signaling pathways. The identification of tPA as an activator of PDGF signaling establishes a novel role for the protease in normal and pathological tissue growth and maintenance, distinct from its well‐known role in plasminogen activation and fibrinolysis.
Platelet‐derived growth factor (PDGF) signaling is critical for normal tissue growth and maintenance, and is mediated through two structurally related tyrosine kinase receptors, PDGFR‐α and PDGFR‐β. The PDGF family of growth factors consist of disulfide‐bonded dimers involving four polypeptide chains: the classical PDGF‐A and PDGF‐B chains (Heldin and Westermark, 1999), and the novel PDGF‐C (Li et al, 2000), and PDGF‐D chains (Bergsten et al, 2001; LaRochelle et al, 2001). Unique for the novel PDGF chains are that they share a two‐domain organization not found within the classical PDGF chains, with an N‐terminal CUB domain in front of the conserved growth factor domain. The role of the CUB domain is currently not fully understood; however, proteolytic removal of the CUB domain is required before PDGF‐CC and PDGF‐DD can bind to and activate their cognate PDGFRs. Activated PDGF‐C, like PDGF‐A, signals through PDGFR‐α, and activated PDGF‐D through PDGFR‐β, whereas PDGF‐B binds to and activates both PDGFRs (Heldin and Westermark, 1999; Li and Eriksson, 2003). Other groups have demonstrated that both PDGF‐C and PDGF‐D are able to activate PDGFR‐α/β heterodimeric complexes as well (Gilbertson et al, 2001; LaRochelle et al, 2001; Cao et al, 2002).
The identity of the proteases responsible for activation of the novel PDGFs remains elusive, although there are data indicating the involvement of serum‐derived factors (Gilbertson et al, 2001; LaRochelle et al, 2001). Here we report that tissue plasminogen activator (tPA) cleaves and activates latent PDGF‐CC. This is a novel role for tPA, which is a secreted serine protease with highly restricted substrate specificity. tPA is best characterized for its role in releasing the broad‐specificity protease plasmin from the inactive zymogen plasminogen (Plg), which then digests the fibrin network of blood clots to form soluble products. Since the activity of tPA is substantially accelerated in the presence of fibrin (Hoylaerts et al, 1982; Ranby, 1982) thereby facilitating a localized generation of plasmin, tPA has been investigated as a potential thrombolytic agent. In fact, tPA is currently the only treatment of acute ischemic stroke approved by the FDA (The National Institute of Neurological Disorders and Stroke rtPA Stroke Study Group, 1995). Recently, there have been several reports suggesting that tPA plays normal and pathological roles that do not require plasminogen (Wu et al, 2000; Nicole et al, 2001; Yepes et al, 2002, 2003), but so far only one other substrate, apart from plasminogen, has been reported for tPA, that is, the NR1 subunit of the NMDA receptor (Nicole et al, 2001). The identification of latent PDGF‐CC as a bona fide substrate for tPA suggests an important role of the protease in PDGF‐mediated signaling.
Identification and cloning of a PDGF‐CC processing protease
In order to identify enzymes capable of activating latent PDGF‐CC, conditioned media from different in vitro‐grown cell lines were screened for expression of endogenous PDGF‐CC, and for the capacity to cleave and activate the secreted latent growth factor. The human fibroblastic cell line AG1523 efficiently secreted full‐length PDGF‐CC, and also displayed the capacity to cleave specifically full‐length PDGF‐C chains, and thus releasing a distinct 22 kDa species under reducing conditions (Figure 1A). This species migrated similarly to the recombinant active growth factor domain of PDGF‐C expressed in insect cells (Li et al, 2000).
In an in vitro assay, the properties of the enzyme(s) involved in cleavage and activation of PDGF‐CC were studied by mixing serum‐free conditioned media from AG1523 cells with His6‐tagged recombinant full‐length PDGF‐CC. Control analysis demonstrated that immunoreactivity toward the His6 epitope was found only in recombinant PDGF‐CC, and not in conditioned medium from AG1523 cells (Figure 1B). SDS–PAGE analysis under reducing and nonreducing conditions, and immunoblotting using an anti‐His6 antibody, showed that increasing amounts of conditioned media from the AG1523 cells sequentially released the CUB domains of latent human PDGF‐CC in a dose‐dependent manner (Figure 1C and D). These data show that the enzymatic activity responsible for the cleavage of full‐length PDGF‐CC is derived from a secreted protease(s) present in the conditioned media from AG1523 cells.
The class of enzyme(s) responsible for cleavage and activation of latent PDGF‐CC was established by generating an enzyme inhibitor profile of the enzymatic activity (Figure 1E). Eight different protease inhibitors (see Figure 1F) were separately preincubated with conditioned media from AG1523 cells, and then incubated with His6‐tagged recombinant full‐length PDGF‐CC. Analysis of the incubation mixtures by SDS–PAGE and immunoblotting revealed that inhibitors of serine proteases (AEBSF, leupeptin, and aprotinin) inhibited the proteolytic cleavage of latent PDGF‐CC (Figure 1E), while inhibitors of other protease classes, including matrix metalloproteinases, failed to inhibit efficiently the processing. These results suggest that a secreted trypsin‐like serine protease is responsible for the proteolytic activation of latent PDGF‐CC.
A coupled reverse transcription–polymerase chain reaction (RT–PCR) assay was employed to clone trypsin‐like serine proteases expressed by AG1523 cells. Based on conserved amino‐acid sequences around the catalytic triad in the serine protease domain, degenerate oligonucleotide mixtures were included in the RT–PCR reactions using single‐stranded cDNA from the AG1523 cells as the template. Amplified products ranging from 500 to 650 bp were visualized by agarose gel electrophoresis (Figure 2A), subcloned, and inserts with the expected size range of approximately 550–600 bp were sequenced. The results revealed that the most abundant amplified cDNA was derived from tPA, while neurotrypsin (NT), coagulation factor X, and trypsinogen IV were other known serine proteases expressed by the AG1523 cells (Figure 2B).
tPA is a specific activator of latent PDGF‐CC
A cotransfection assay was established to identify serine proteases able to cleave and activate latent PDGF‐CC. Expression plasmids encoding the relevant enzymes and full‐length PDGF‐C were cotransfected into COS‐1 cells, and aliquots of the conditioned media from the transfectants were subjected to SDS–PAGE and immunoblotting using antibodies to the growth factor domain of PDGF‐C. The results showed that tPA released the growth factor domain of latent PDGF‐CC, and the fragment migrated as a 22 kDa species under reducing conditions (Figure 3A). In contrast, NT lacked proteolytic activity toward latent PDGF‐CC. As a specificity control, we analyzed the ability of tPA and NT to use full‐length PDGF‐DD as the substrate in the cotransfection assay. The results revealed that neither of the two enzymes was able to cleave and activate latent PDGF‐DD (Figure 3B). Using purified tPA and recombinant latent PDGF‐CC, or recombinant latent PDGF‐DD, in an in vitro assay, we confirmed these observations showing that PDGF‐CC, but not PDGF‐DD, is a substrate for tPA (Figure 3C and D). One difference in the latter results, as compared with the results from the cotransfection assay, was that purified tPA generated a second intermediate species of 32 kDa using latent PDGF‐CC as the substrate. It is possible that this intermediate is the result of digestion by plasmin contamination in the tPA preparation, since the size of the fragment is similar to that of plasmin‐cleaved PDGF‐CC previously reported (Li et al, 2000).
To ensure that the cleavage of PDGF‐C observed in the cotransfection assay was a direct effect of tPA, and not an indirect effect due to cleavage by remnants of plasmin, we cultured the COS‐1 cells in the absence or presence of the specific plasmin inhibitor α2‐anti‐plasmin or in Plg‐depleted medium prior to transfection (Figure 3E). Neither α2‐anti‐plasmin treatment nor culturing in Plg‐depleted medium had any effect on the processing of PDGF‐C, showing that the cleavage of PDGF‐C is performed by tPA directly.
To demonstrate that the proteolytic activity of tPA accounted for the major PDGF‐CC processing activity produced by AG1523 cells, a well‐characterized inhibitor of tPA, tPA‐STOP™ (Sturzebecher et al, 1997), and the serine protease inhibitor aprotinin (see above) were added to the serum‐free culture medium of growing AG1523 cells. Analysis of conditioned media showed that tPA‐STOP™, in a dose‐dependent way, prevented processing of full‐length PDGF‐CC (Figure 4A). Similarly, aprotinin efficiently inhibited processing of latent PDGF‐CC in comparison with the untreated control. These results showed that tPA accounts for a majority of the PDGF‐CC processing activity in conditioned media from AG1523 cells.
We also examined the ability of primary cultures of lung and kidney fibroblasts from wild‐type and tPA‐deficient mice to produce and activate latent PDGF‐CC. SDS–PAGE and immunoblotting analyses of TCA‐precipitated proteins from serum‐free conditioned media showed that the primary fibroblasts secreted latent PDGF‐CC migrating as a 48 kDa species in SDS–PAGE under reducing conditions (Figure 4B). In the medium from wild‐type cells, processing of latent PDGF‐CC into species migrating as 35 kDa species and as double bands of 22–25 kDa was seen. In contrast, in medium from tPA‐deficient cells, the generation of double species migrating as 22–25 kDa was reduced to less than 10%, and the intensity of the 35 kDa species was also significantly reduced. These data demonstrate an essential role of tPA in activation of latent PDGF‐CC in vivo.
tPA‐mediated activation of PDGF‐CC generates a PDGFR‐α agonist
We verified that the growth factor domain in PDGF‐CC released by tPA‐mediated proteolysis is an efficient PDGFR‐α ligand. Conditioned media from transfected COS‐1 cells were applied onto porcine aortic endothelial (PAE) cells with stable expression of PDGFR‐α (Figure 5A). Stimulation of the cells using conditioned medium from mock‐transfected COS‐1 cells, or media from transfected COS‐1 cells separately expressing tPA, or latent PDGF‐CC, failed to induce receptor activation measured as induction of receptor tyrosine phosphorylation. In contrast, stimulation using medium from COS‐1 cells coexpressing tPA and full‐length PDGF‐CC induced strong PDGFR‐α activation. This showed that the growth factor domain of full‐length PDGF‐CC released by tPA is a bona fide ligand and activator of PDGFR‐α.
The possibility of a direct protein–protein interaction between tPA and latent PDGF‐CC was explored by developing a pull‐down assay. Ni‐NTA beads were allowed to bind recombinant His6‐tagged latent PDGF‐CC or PDGF‐DD, and purified tPA was added and incubated. Following extensive washings, bound proteins were subsequently eluted with an imidazole‐containing buffer, and the eluates were analyzed by immunoblotting using specific antibodies. The results showed that full‐length PDGF‐CC‐coated beads specifically bound tPA, while uncoated Ni‐NTA beads or PDGF‐DD‐coated beads failed to do so (Figure 5B). Similar experiments using Ni‐NTA beads separately coated with recombinant ‘free’ CUB domain or recombinant core domain of PDGF‐CC showed that both domains were able to interact with tPA.
We have previously identified a conserved site of four amino acids containing three basic amino‐acid residues (amino‐acid residues ‐R‐K‐S‐R‐) as a potential site for proteolytic activation of latent PDGF‐CC (Li et al, 2000). It is notable that the corresponding regions in PDGF‐A and PDGF‐B are the cleavage sites for furine‐like proteases that act in the exocytic pathway during secretion of these PDGFs (Östman et al, 1992; Siegfried et al, 2003). To verify this, a mutant with the tribasic site replaced with alanine residues was created (schematically illustrated in Figure 5C). Analysis using the cotransfection assay verified that the mutant was resistant to tPA‐mediated cleavage, while the wild‐type PDGF‐CC was readily cleaved (Figure 5D). These data suggest that tPA cleaves latent PDGF‐CC in, or at least around, the conserved tribasic site.
tPA‐dependent activation of latent PDGF‐CC drives proliferation of primary fibroblasts
We observed that primary fibroblasts derived from tPA‐deficient mice grew slower in culture than fibroblasts derived from wild‐type animals, raising the possibility that activation of latent PDGF‐CC by tPA generated autocrine and paracrine growth stimulatory loops for primary fibroblasts in culture. To analyze this effect, isolated wild‐type and tPA‐deficient fibroblasts were serum‐starved overnight, and the growth of the cells during the next 24 h was monitored using an enzyme‐based viability assay (see Materials and methods). The results confirmed our initial observation and showed that tPA‐deficient cells displayed a reduced growth rate in serum‐free medium as compared to wild‐type cells (Figure 6A). Rescue of the tPA‐deficient cells by the addition of 50 ng ml−1 of activated PDGF‐CC or recombinant tPA to the serum‐free culture medium allowed the cells to grow similar to the wild‐type fibroblasts.
To further demonstrate that growth of primary fibroblasts in culture was dependent on a tPA‐mediated growth stimulatory loop, serum‐starved fibroblast cultures were labeled with 5‐bromo‐2′‐deoxyuridine (BrdU) for 24 h in order to identify dividing cells. Cell nuclei were visualized with 4′,6‐diamidine‐2′‐phenylindole dihydrochloride (DAPI), and BrdU‐labeled cells were determined by immunofluorescence using antibodies to BrdU (Figure 6B). Quantification of the results showed that the fraction of BrdU‐labeled nuclei were significantly higher in wild‐type fibroblasts as compared to the tPA‐deficient cells (Figure 6C). Addition of 50 ng ml−1 of activated PDGF‐CC or recombinant tPA strongly stimulated BrdU incorporation in the tPA‐deficient cells but had less effect on wild‐type cells. These data suggest that autocrine and paracrine growth stimulatory loops are present in primary fibroblasts, and that these loops are generated by a tPA‐mediated activation of latent PDGF‐CC.
It is known that constitutive activation of PDGFRs by PDGFs leads to receptor desensitization (Heldin and Westermark, 1999), and we therefore investigated whether the differences observed in growth between the wild‐type and tPA‐deficient fibroblasts upon PDGF‐CC treatment were due to differential activation of PDGFR‐α. Recombinant PDGF‐CC protein was applied onto the primary fibroblasts and receptor activation was measured as induction of PDGFR‐α tyrosine phosphorylation (Figure 6D, upper panel). Stimulation of PDGFR‐α was more pronounced in the tPA‐deficient cells as compared to wild type, which might explain the efficient stimulation of proliferation seen in these cells following PDGF‐CC treatment.
To determine whether PDGF‐C and tPA are coexpressed, or at least expressed in adjacent cells, both proteins were localized by immunohistochemistry using tissue sections from E14.5 mouse embryos and T241 tumor xenografts (Figure 7). Furthermore, the expressions of PDGF‐C and tPA reported in previous publications were compiled and compared (Carroll et al, 1994; Ding et al, 2000; Aase et al, 2002). The results from these analyses suggest that PDGF‐C and tPA are coexpressed in several locations in the developing embryo such as the kidney and the surface ectoderm of the skin (Figure 7A–D). In tumor tissue sections, PDGF‐C expression was observed mostly in tumor cells located at the center of the tumor in close apposition to larger blood vessels, while tPA was mainly expressed in the endothelium of the tumor blood vessels (Figure 7E and F). Scattered PDGF‐C‐positive tumor cells were also seen at the edge of the tumor. These observations support our findings and suggest that PDGF‐CC can be activated by tPA in vivo.
It is well established that PDGF‐CC expression is widespread in both normal adult and embryonic tissues, as well as in several pathological conditions including tumors. In order to understand the physiological roles of PDGF‐CC‐mediated signal transduction in these processes, it is important to gain further insight into how latent PDGF‐CC becomes proteolytically activated to generate a PDGF receptor agonist. We have previously shown that the relatively nonspecific protease plasmin can be used to activate both PDGF‐CC and PDGF‐DD from their latent precursors (Li et al, 2000; Bergsten et al, 2001); however, given the wide substrate specificity of plasmin, we considered this protease unlikely to be the physiologically relevant protease in activation of the novel PDGFs. Elucidating the identity, localization, and regulation of this protease(s) will greatly enhance our understanding of PDGF regulation in vivo.
To identify the enzyme responsible for activation of latent PDGF‐CC, we developed an in vitro assay to monitor cleavage of latent PDGF‐CC, and by using a combination of protease inhibitor profiling (so‐called reverse biochemistry; Takeuchi et al, 1999), molecular cloning with RT–PCR using degenerate primers, and a functional assay, we identified tPA as a specific protease able to activate latent PDGF‐CC. Despite the close structural similarities between PDGF‐C and PDGF‐D, the latter factor was not activated by tPA, demonstrating that distinct pathways are involved in activation of the two factors.
tPA is a multidomain trypsin‐like serine protease best known for its role in fibrinolysis via proteolytic activation of plasminogen into plasmin (for reviews, see Vassalli et al, 1991; Collen, 2001). However, the expression pattern of tPA in the mouse embryo, especially in neuronal tissue and in areas undergoing extensive tissue remodeling, suggests that the protease may serve additional functions (Rickles and Strickland, 1988; Carroll et al, 1994). Also, several reports have suggested that tPA plays normal and pathological roles that do not require plasminogen activation (Strickland, 2001; Tsirka, 2002), but apart from plasminogen, only one additional substrate has been identified, that is, the NR1 subunit of the NMDA receptor (Nicole et al, 2001). Our identification of tPA as a specific activator of latent PDGF‐CC is thus rather unexpected, but it provides additional evidence for roles of tPA in nonthrombolytic events, including fibrosis, angiogenesis, and tumor growth (see below).
The mechanisms underlying the specific cleavage and activation of latent PDGF‐CC by tPA involve the formation of a stable substrate·protease complex. Our data show that tPA specifically interacts with both the CUB and the PDGF/VEGF‐like growth factor domain in PDGF‐CC. The specific binding of tPA to the CUB domain of PDGF‐C, and not that of PDGF‐D, is required for proteolytic activation of the factor (our unpublished observation). Thus, the role of the CUB domain in PDGF‐CC appears two‐fold: to prevent an agonistic role of the unprocessed growth factor (Li et al, 2000) and to bind specifically tPA to allow a site‐specific cleavage of the factor. It is well documented that CUB domains in different proteins are involved in protein·protein interactions (e.g., see Thielens et al, 1999; Nakamura and Goshima, 2002), and it is possible that the released CUB domains might act as a competitive inhibitor in the activation of latent PDGF‐CC. At present, the structural domains of tPA interacting with the CUB domain of PDGF‐C are unknown. In Figure 8A, we have summarized our findings regarding the complex formation of full‐length PDGF‐CC and tPA, and the functional consequences of the growth factor when only one or both CUB domains have been removed by tPA‐mediated proteolysis.
The tight complex formation of tPA and PDGF‐CC allows a precise cleavage of the substrate. Previously, we suggested that a conserved tribasic region (amino‐acid residues ‐R231‐K232‐S233‐R234‐ in human PDGF‐C), 15 amino‐acid residues N‐terminal of the first cysteine in the PDGF/VEGF‐like domain, represented a putative proteolytic cleavage site (Li et al, 2000). This suggestion was based on the location of this site in relation to the well‐defined cleavage sites found in the intracellular proforms of PDGF‐A and PDGF‐B. Our data verify that the corresponding site in PDGF‐C is the cleavage site for tPA.
Components of the fibrinolytic system, including tPA, urokinase‐type plasminogen activator (uPA), the urokinase‐type plasminogen activator receptor (uPAR), and the plasminogen activator inhibitors (PAIs), are often overexpressed in tumors (Kwaan, 1992 and references therein). So far, strong evidence suggests that overexpression of uPA, uPAR, and PAIs is linked to increased tumor growth, invasion, and metastatic spreading, whereas less is known about the role of tPA in these processes. In addition, many types of tumors overexpress PDGF‐C (Uutela et al, 2001; Zwerner and May, 2001; Andrae et al, 2002; Dijkmans et al, 2002; Lokker et al, 2002; U Eriksson, unpublished observation), raising the possibility that in PDGF‐C‐expressing tumors, tPA would contribute to the activation of the growth factor. Several studies have shown that PDGF‐C overexpression in tumor cells enhances tumor growth by promoting cellular transformation, and stimulates stromogenesis and tumor vascularization (Zwerner and May, 2001; Cao et al, 2002; Li et al, 2003). The source of tPA could either be PDGF‐CC‐expressing tumor cells themselves or as shown here for the T241 tumor the enzyme may be released by the invading endothelial cells of the tumor vasculature (Figure 7F).
tPA administration is the only FDA‐approved thrombolytic therapy for acute ischemic stroke, and increasing evidence from studies in animal models of embolic stroke cautions against the use of tPA, as it might mediate neuronal damage (Tsirka, 2002). At least part of the neuronal damage might be caused by a tPA‐dependent, plasminogen‐independent opening of the blood–brain barrier mediated via the low‐density lipoprotein receptor‐related protein (LRP) and the cleavage of an as yet unidentified substrate (Yepes et al, 2003). Interestingly, LRP is a negative regulator of PDGF signaling (Boucher et al, 2003), raising the possibility that part of the plasminogen‐independent action of tPA is indeed mediated via modulation of PDGF signaling.
The finding that the growth of fibroblasts is dependent on a tPA‐mediated activation of latent PDGF‐CC, thus generating autocrine and paracrine growth stimulatory loops, raises several possible roles of PDGF‐CC in normal and pathological conditions involving fibroblast growth and recruitment. For example, such conditions may include tissue morphogenesis and regeneration, wound healing, and tumor growth (see Figure 8B). In part, this mechanism may also be the explanation for the long‐standing observation that it is relatively easy to establish primary cultures of fibroblasts in comparison to most other cell types.
In summary, the identification of tPA as a potent activator of latent PDGF‐CC has provided novel insights into PDGF‐mediated signaling with broad implications in normal and pathological conditions, in particular in tumor biology and cardiovascular medicine.
Materials and methods
All cells used were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (FCS), 2 mM glutamine, 100 U ml−1 penicillin, and 100 μg ml−1 streptomycin, except PAE cells that were kept in supplemented F12 medium. The cells were cultured at 37°C in a humidified 5% CO2 atmosphere. Kidney and lung primary fibroblast cultures were prepared essentially as described (Eghbali et al, 1991) from 5‐week‐old wild‐type (+/+) and tPA‐deficient (−/−) mice (Carmeliet et al, 1994) (kindly provided by Prof. P Carmeliet, Leuven). In short, kidneys and lungs were dissected, washed in ice‐cold PBS, cut into smaller pieces, and incubated with trypsin/collagenase in PBS for 20 min at 37°C. Dissociated cells were pelleted and plated. Experiments were performed on cells at passages 4–7.
Protein expression and immunoblotting
To test the endogenous expression of PDGF‐CC, subconfluent AG1523 cells and primary fibroblast cultures were cultured in serum‐free DMEM overnight. Recombinant His6‐tagged human PDGF‐CC species and full‐length PDGF‐DD were expressed in serum‐free medium from Sf9 insect cells using the baculovirus expression system as described previously (Li et al, 2000; Bergsten et al, 2001). To explore the extracellular proteolytic activities in conditioned serum‐free AG1523 medium, the medium was coincubated with recombinant latent PDGF‐CC‐containing medium (ratios 1:2, 3:2, and 10:2) at 37°C overnight. To identify PDGF‐CC activating serine proteases, the protease expression constructs were cotransfected with full‐length PDGF‐C (Li et al, 2000), full‐length PDGF‐D (Bergsten et al, 2001), or PDGF‐C cleavage site mutant constructs into subconfluent COS‐1 cells using LipofectaminePlus in serum‐free DMEM (Life Technology). Transfection with empty vectors served as negative control. After 24 h, the transfection medium was replaced by DMEM only, with or without the addition of α2‐anti‐plasmin (10 ng–1 μg, #4030, American Diagnostica Inc.), for an extra 24 h. In addition, the COS‐1 cells were grown in DMEM supplemented with 10% Plg‐depleted FCS prior to transfection. Plg was removed from the FCS by affinity chromatography on lysine‐Sepharose (Deutsch and Mertz, 1970) and the Plg‐depleted FCS was tested by immunoblotting with rabbit anti‐human Plg (A0081, DAKO). The conditioned serum‐free medium was collected, and proteins were TCA precipitated as described previously (Li et al, 2000). In the case of the primary cultures, total protein concentration was measured and normalized (Bradford, 1976). All precipitates were subjected to SDS–PAGE under reducing conditions if not stated otherwise, immunoblotting, and visualization by chemiluminescence. PDGF‐C and PDGF‐D were detected by immunoblotting using affinity‐purified polyclonal rabbit antibodies against PDGF‐C (Li et al, 2000) and PDGF‐D (Bergsten et al, 2001), respectively. The His6‐tagged proteins were detected using an anti‐His monoclonal antibody (C‐terminal, Invitrogen). tPA was detected using sheep polyclonal antibodies against human tPA (ab9030, Abcam).
All protease inhibitors were purchased from Sigma and the concentrations used were as follows: AEBSF 1 mM, bestatin 100 μM, leupeptin 100 μM, pepstatin A 10 μM, E64 100 μM, aprotinin 100 μM (∼3 TIU), EDTA 50 mM, and phosphoramidon 100 μM. The protease inhibitors were preincubated with conditioned AG1523 medium at room temperature for 30 min, and then incubated with recombinant PDGF‐CC (ratio 10:2) at 37°C overnight. Recombinant PDGF‐CC species were analyzed by immunoblotting as above.
To determine whether tPA is the major proteolytic enzyme responsible for the PDGF‐CC processing in AG1523 conditioned medium, AG1523 cells were cultured in serum‐free medium, with or without the addition of a synthetic tPA inhibitor tPA‐STOP™ (3.5–35 μM, #544, American Diagnostica Inc.) or 100 μM aprotinin as a positive control. The conditioned serum‐free medium was collected, and proteins were precipitated before SDS–PAGE and immunoblotting using antibodies against PDGF‐C (see above).
Cloning of serine proteases and plasmid construction
To clone trypsin‐like serine proteases in AG1523 fibroblastic cells, total cellular RNA was prepared using the guanidinium thiocyanate/acid phenol method (Chomczynski and Sacchi, 1987). Single‐stranded cDNA was synthesized using AMV Reverse Transcriptase (Amersham) and oligo‐dT to prime the reaction. Degenerate oligonucleotide primers flanking the conserved histidine and serine residues in the catalytic triad were designed as follows: 5′‐CAR TGG GTN YTN WCN GCN GCN CAY TG (corresponding to the amino‐acid sequence Q W V L/F S/T A A H C, forward) and 5′‐NCC NCC NGA RTC NCC YTG RCA NGC RTC (corresponding to the amino‐acid sequence D A C Q G D S G G, reverse). The oligonucleotides were used to prime PCRs utilizing cDNA from the AG1523 cells as template. The PCR products were cloned into the pCR2.1‐TOPO vector (TOPO TA Cloning kit, Invitrogen) and clones of the expected size of 500–600 bp were sequenced.
Full‐length human tPA was amplified by PCR using cDNA from the AG1523 cells as template and the 1750‐bp product was subcloned into the pCR2.1‐TOPO vector. The primers used, including a BamH1 site (underlined), were as follows: 5′‐CGGGATCCGCCGTGAATTTAAGGGAC (forward) and 5′‐CGGGATCCTTGCTTTTGAGGAGTCGG (reverse). The BamH1 fragment was excised and cloned into the eukaryotic expression vector pSG5.
The fully sequenced MGC clone containing the 5′ part of human NT in the pOTB7 vector was purchased from Research Genetics whereas the 3′ part was amplified by PCR using AG1523 cDNAs as template. The primers used were as follows: 5′‐GAGCTGAATACATACGTG (forward) and 5′‐GCAGATCTGCTGCTTTGAAGTTTCCA (reverse, including a BglII site, underlined). The resulting 1400‐bp 3′ fragment was subcloned into the pCR2.1‐TOPO vector and then excised with NdeI–BglII. A full‐length cDNA for hNT was constructed by fusing the excised 3′ fragment with NdeI–BglII‐digested 5′‐hNT/pOTB7. The full‐length cDNA for hNT was excised and directionally cloned into the EcoRI–BglII sites of the eukaryotic expression vector pSG5.
To generate the cleavage site mutant, mouse PDGF‐C cDNA was used as template. The predicted processing site in murine PDGF‐C, amino‐acid residues ‐K‐K‐S‐K‐, was replaced by four alanines. The N‐terminal fragment of PDGF‐C, containing an EcoRI and a NotI site (underlined), and the C‐terminal fragment, containing a NotI and an XbaI site (underlined), were amplified using the following primers: 5′‐GGAATTCAGCCAAATGCTCCTCCTCGGCCTC (forward, N‐terminal) and 5′‐TGCCGCGGCCGCCCCATACAGGAAAGCCTT (reverse, N‐terminal, alanine replacement in bold), 5′‐GCGGCCGCGGCAGTGGTGAATCTGAATCTCCTC (forward, C‐terminal, alanine replacement in bold), and 5′‐GCTCTAGACTGCAGTTACCCTCCTGCGTT (reverse, C‐terminal). The amplified fragments were ligated and cloned in‐frame into pcDNA3.1 (+) expression vector.
To produce recombinant CUB domain of human PDGF‐C using the baculovirus system, the sequence encoding amino‐acid residues 23–163 of PDGF‐C was amplified by PCR. Primers used were as follows: 5′‐CGGGATCCCGAATCCAACCTGAGTAG (forward, including a BamHI site for in‐frame cloning) and 5′‐CCGGAATTCCTAATGGTGATGGTGATGATGTTTGTCATCGTCGTCGACAATGTTGTAGTG (reverse, including an EcoRI site and sequences encoding a C‐terminal His6 tag). The amplified product was cloned into the baculovirus expression vector pAcGP67A.
All primers used were purchased from Invitrogen and all the constructs were verified by nucleotide sequencing. The nucleotide and amino‐acid sequences of human tPA can be found in the GenBank under accession number NM_000930 and of hNT under accession number NM_003619. The MGC clone containing the 5′ part of hNT has GenBank accession ID BC007761.
In vitro cleavage and protein–protein interaction studies
Recombinant latent PDGF‐CC and PDGF‐DD were digested with human tPA in 100 mM Tris–HCl pH 7.5, 0.1% Tween 20, and 0.1 mg ml−1 CNBr activated fibrinogen (Sigma) for 4 h at 37°C using 0.2–20 μg ml−1 tPA purified from human melanoma cells (T7776, Sigma). The digestions were analyzed by SDS–PAGE under reducing conditions and immunoblotted using affinity‐purified antibodies against PDGF‐C and PDGF‐D, respectively (see above).
To determine a direct protein–protein interaction between tPA and PDGF‐CC, His6‐tagged recombinant protein species were bound to Ni‐NTA‐agarose (Qiagen) and then incubated with 1 μg of purified tPA for 2 h at room temperature. Uncoated and PDGF‐DD‐coated Ni‐NTA beads were used as controls. The beads were washed thoroughly, and His6‐tagged proteins were specifically eluted with 400 mM imidazole. Eluted proteins were analyzed by SDS–PAGE under reducing conditions and immunoblotted with antibodies against human tPA (see above). The membranes were subsequently stripped and reprobed with specific antibodies.
Receptor activation and proliferation analysis
To monitor growth factor‐induced tyrosine phosphorylation of PDGFR‐α, serum‐starved PAE cells stably expressing human PDGFR‐α were incubated for 120 min on ice with conditioned medium from COS‐1 cells transfected with full‐length PDGF‐C in the absence or presence of tPA. Alternatively, primary wild‐type and tPA‐deficient fibroblasts were stimulated with 100 ng ml−1 activated PDGF‐CC protein. The cells were lysed as described previously (Li et al, 2000) and PDGFR‐α was immunoprecipitated using a specific antiserum (Eriksson et al, 1992). Precipitated proteins were separated by SDS–PAGE under reducing conditions. Tyrosine‐phosphorylated receptors were detected by immunoblotting using an antiphosphotyrosine antibody (PY99, Santa Cruz). The membranes were stripped and reprobed using a polyclonal antibody against the C‐terminal of the PDGFRs (CED) to detect receptor expression levels.
To monitor cell growth, both the cell proliferation reagent WST‐1 (Roche) and BrdU (Sigma) were used. A total of 0.4 × 104 (WST‐1) or 1 × 104 (BrdU) wild‐type and tPA‐deficient fibroblasts were seeded in triplicate–hexaplicate, and after attachment they were serum‐starved overnight. Serum‐starved cells were counted (WST‐1 seeding control) and alternatively incubated for 24 h in serum‐free medium supplemented with 1 mg ml−1 BSA, and 50 μM BrdU in the BrdU experiment, in the absence or presence of 50 ng ml−1 activated PDGF‐CC or tPA protein (#116, American Diagnostica Inc.). Upon counting, WST‐1 reagent was added and measured according to the manufacturer's protocol using an ELISA reader. In the BrdU experiment, the cells were fixed in 4% paraformaldehyde in PBS for 30 min at room temperature and the DNA was denatured in 2 M HCl for 20 min at room temperature and then blocked in 0.5% BSA, 0.5% Tween, and 10% goat serum in PBS. BrdU was localized by a monoclonal anti‐BrdU antibody (DAKO), and proliferating cells were visualized by an Alexa594‐conjugated mouse secondary antibody (Molecular Probe). To visualize all nuclei, DAPI (1 μg ml−1, Roche) was included in the secondary antibody solution. Quantification of the BrdU‐positive cells was performed by counting all cells along the vertical and horizontal diameters of all wells.
Immunohistochemical analysis of PDGF‐C and tPA expression
Expression analysis of PDGF‐C and tPA was performed by immunohistochemistry using tissue sections from E14.5 mouse embryos and T241 tumor xenografts generated from syngenic mice essentially as described previously (Aase et al, 2002). The primary antibodies used were affinity‐purified rabbit antibodies directed against human PDGF‐C and rabbit anti‐mouse tPA IgG (#387, American Diagnostica Inc.). As negative controls, the sections were incubated only with secondary Ig or preimmune rabbit IgG, and in all cases only background staining was observed.
We thank Erika Folestad for the kind gifts of recombinant PDGF‐D and antibodies to PDGF‐D, and Peter Carmeliet for the gift of tPA‐deficient mice. This work was supported by grants from The Novo Nordisk Foundation, The Swedish Research Council, and Karolinska Insitutet.
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