Smac/DIABLO is a mitochondrial protein that potentiates some forms of apoptosis, possibly by neutralizing one or more members of the IAP family of apoptosis inhibitory proteins. Smac has been shown to exit mitochondria and enter the cytosol during apoptosis triggered by UV‐ or γ‐irradiation. Here, we report that Smac/DIABLO export from mitochondria into the cytosol is provoked by cytotoxic drugs and DNA damage, as well as by ligation of the CD95 death receptor. Mitochondrial efflux of Smac/DIABLO, in response to a variety of pro‐apoptotic agents, was profoundly inhibited in Bcl‐2‐overexpressing cells. Thus, in addition to modulating apoptosis‐associated mitochondrial cytochrome c release, Bcl‐2 also regulates Smac release, suggesting that both molecules may escape via the same route. However, whereas cell stress‐associated mitochondrial cytochrome c release was largely caspase independent, release of Smac/DIABLO in response to the same stimuli was blocked by a broad‐spectrum caspase inhibitor. This suggests that apoptosis‐associated cytochrome c and Smac/DIABLO release from mitochondria do not occur via the same mechanism. Rather, Smac/DIABLO efflux from mitochondria is a caspase‐catalysed event that occurs downstream of cytochrome c release.
Apoptosis is a cell‐autonomous programmed cell death mechanism that is utilized extensively during development, tissue homeostasis and maintenance of the immune system in adult organisms (Jacobson et al., 1997). The morphological changes typical of apoptosis, characterized by cell shrinkage, plasma membrane blebbing, chromatin compaction and nuclear fragmentation, are orchestrated by the activity of a family of cysteine proteases called caspases (Kerr et al., 1972; Martin and Green, 1995; Earnshaw et al., 1999).
One important route to caspase activation involves the translocation of cytochrome c from the mitochondrial intermembrane space into the cytosol (Liu et al., 1996). Following release from mitochondria, cytochrome c promotes the assembly of a protein complex called the apoptosome, which includes caspase‐9 bound to the CED‐4 homologue Apaf‐1 (Li et al., 1997; Zou et al., 1997; Adrain et al., 1999; Rodriguez and Lazebnick, 1999; Cain et al., 2000; Adrain and Martin, 2001). Upon activation, caspase‐9 instigates a proteolytic cascade involving multiple caspases, which culminates in the cleavage of numerous substrate proteins and, ultimately, in macrophage engulfment of apoptotic corpses (Srinivasula et al., 1998; Earnshaw et al., 1999; Nicholson, 1999; Slee et al., 1999a,b, 2001; Savill and Fadok, 2000).
Precisely how the efflux of pro‐apoptotic molecules from the mitochondrial intermembrane space occurs is not clear at present (for recent reviews, see Desagher and Martinou, 2000; Adrain and Martin, 2001; Martinou and Green, 2001; Zamzami and Kroemer, 2001). However, accumulating evidence suggests a model whereby the death‐promoting ‘BH‐3‐only’ members of the Bcl‐2 family, such as caspase‐cleaved Bid (tBid), induce the homo‐oligomerization of death promoting molecules Bax and/or Bak and their insertion into the outer mitochondrial membrane (Li et al., 1998; Gross et al., 1999; Desagher and Martinou, 2000; Wei et al., 2000; Antonsson et al., 2001; Martinou and Green, 2001; Wei et al., 2001). Several lines of evidence suggest that Bax or Bak oligomers may form cytochrome c‐conducting channels within the mitochondrial outer membrane (Antonsson et al., 2000; Saito et al., 2000). Bcl‐2, a key inhibitor of apoptosis, blocks cytochrome c release, possibly by preventing Bax/Bak oligomerization and consequent pore formation (Kluck et al., 1997; Yang et al., 1997; Mikhailov et al., 2001).
Recently, a novel mitochondria‐derived pro‐apoptotic molecule called Smac/DIABLO was identified (Du et al., 2000; Verhagen et al., 2000). Based largely upon in vitro studies, Smac/DIABLO appears to function by neutralizing the caspase‐inhibitory properties of the IAP family of proteins, particularly XIAP (Roy et al., 1997; Deveraux et al., 1998; Du et al., 2000; Verhagen et al., 2000). Current data suggest a model whereby the ability of XIAP to repress active caspase‐9 within the apoptosome complex is overcome by displacement of XIAP from caspase‐9 by Smac/DIABLO (Ekert et al., 2001; Srinivasula et al., 2001).
Similar to cytochrome c, Smac/DIABLO is encoded by a nuclear gene and is subsequently imported into mitochondria (Stuart and Neupert, 1990; Du et al., 2000; Verhagen et al., 2000). During mitochondrial import, the N‐terminus of Smac/DIABLO is removed by limited proteolysis to generate the mature form of the molecule (Chai et al., 2000; Du et al., 2000). Interestingly, mature Smac/DIABLO exists as a dimer, mediated by a hydrophobic interface within the N‐termini of individual Smac/DIABLO molecules (Chai et al., 2000). Mutations that disrupt Smac/DIABLO dimer formation abrogate the XIAP‐neutralizing ability of this molecule, suggesting that Smac/DIABLO dimerization is essential for its pro‐apoptotic activity (Chai et al., 2000). Because the Smac/DIABLO dimer behaves as an ∼100 kDa molecule, whereas cytochrome c has a mol. wt of ∼12 kDa, an important question that remains unresolved is whether the channel responsible for mediating cytochrome c release is large enough to accommodate Smac/DIABLO (Chai et al., 2000; Du et al., 2000; Martinou and Green, 2001).
In this study, we generated a Smac/DIABLO‐specific antibody to examine the efflux of endogenous Smac/DIABLO from mitochondria in response to diverse pro‐apoptotic stimuli. Using a range of cytotoxic drugs/DNA‐damaging stimuli, we show that mitochondrial Smac/DIABLO release is a general feature of apoptosis, and that Bcl‐2 regulates this event. However, in sharp contrast to the behaviour of cytochrome c during stress‐associated apoptosis, we find that Smac/DIABLO release from mitochondria is largely attenuated by caspase inhibition. Thus, Smac/DIABLO efflux from mitochondria is a caspase‐catalysed event that occurs downstream of cytochrome c translocation.
Generation and characterization of Smac/DIABLO‐specific antisera
To explore Smac/DIABLO redistribution from mitochondria to the cytosol during apoptosis, we generated polyclonal antibodies against this protein by immunizing rabbits with bacterially expressed polyhistidine‐tagged human Smac/DIABLO (Du et al., 2000). Following affinity purification, the specificity of the antiserum was confirmed by the detection of endogenous and overexpressed Smac/DIABLO in HEK 293T whole‐cell lysates (Figure 1A).
We also examined Smac/DIABLO expression in a panel of adult human tissues and in tumour cell lysates (Figure 1B and C). Consistent with data relating to Smac/DIABLO mRNA distribution in adult human tissues (Du et al., 2000), mature Smac/DIABLO protein was detected in the majority of tissues examined (Figure 1B). Smac/DIABLO was also ubiquitously expressed in a panel of tumour cell lines, including those derived from monocytic, T lymphocytic and B lymphocytic lineages, and in epitheloid‐like cells (Figure 1C).
Smac/DIABLO release from mitochondria is a general feature of apoptosis
Previous studies have reported the translocation of Smac/DIABLO from mitochondria to the cytosol in cells stimulated to undergo apoptosis by UVB‐irradiation, γ‐irradiation or glucocorticoids (Du et al., 2000; Verhagen et al., 2000; Chauhan et al., 2001; Ekert et al., 2001). However, it is unclear whether Smac/DIABLO release from mitochondria, like that of cytochrome c, is a general feature of apoptosis (Liu et al., 1996; Kluck et al., 1997; Yang et al., 1997; Bossy‐Wetzel et al., 1998; Li et al., 1998; Luo et al., 1998; Goldstein et al., 2000; Wei et al., 2000). To explore this, we treated Jurkat cells with a panel of pro‐apoptotic agents established to provoke cytochrome c release and apoptosis (Figure 2). Cells treated with staurosporine, actinomycin D and daunorubicin readily exhibited features typical of apoptosis, including nuclear condensation/fragmentation, activation of caspase‐3 and cleavage of the caspase‐3 substrate gelsolin (Figure 2A and B).
Using a digitonin‐based subcellular fractionation procedure, we analysed cytosolic and mitochondrial fractions obtained from these cells for the presence of cytochrome c and Smac/DIABLO, respectively (Figure 2C). Whereas untreated cells exhibited negligible redistribution of cytochrome c from mitochondria into the cytosol, cytochrome c was present in the cytosols of dying cells, as expected (Figure 2C). Smac/DIABLO similarly accumulated in the cytosols of dying cells and was lost from the mitochondrial pellet fractions (Figure 2C). Thus, taken together with previous reports on Smac/DIABLO release in the context of UV‐ and γ‐irradiation, redistribution of Smac/DIABLO appears to be a general feature of apoptosis.
Cytochrome c and Smac/DIABLO release occur with similar kinetics in death receptor‐induced apoptosis
In certain cells such as hepatocytes, apoptosis initiated via the CD95 (Fas/APO‐1) death receptor requires engagement of the mitochondrial pathway in order to complete the apoptotic programme (Scaffidi et al., 1998). In this context, cytochrome c release is achieved via caspase‐8‐mediated cleavage of Bid and the subsequent translocation of the Bid C‐terminus to mitochondria (Li et al., 1998; Luo et al., 1998; Gross et al., 1999). To explore whether death receptor‐initiated apoptosis was also associated with Smac/DIABLO redistribution to the cytosol, Jurkat cells were treated with agonistic anti‐Fas antibody, followed by assessment of cytochrome c and Smac/DIABLO release from mitochondria. Figure 3 illustrates that ligation of the CD95 receptor provoked extensive apoptosis, which was accompanied by the accumulation of cytochrome c in the cytosol and its loss from mitochondrial pellets. Smac/DIABLO was also detected in cytosolic fractions at all timepoints where cytochrome c was observed, beginning 3 h after treatment with anti‐Fas antibodies (Figure 3B). These data demonstrate that cytochrome c and Smac/DIABLO are both released from mitochondria in response to death receptor ligation, with similar kinetics.
Bcl‐2 blocks Smac/DIABLO release provoked by diverse pro‐apoptotic agents
Given their common location within the mitochondrial intermembrane space and their mutual efflux in response to divergent pro‐apoptotic stimuli (Figures 2 and 3), it is possible that the pore which enables cytochrome c efflux also permits Smac/DIABLO release. However, whereas cytochrome c has a mol. wt of ∼12 kDa, Smac/DIABLO has been reported to form ∼100 kDa dimers in solution (Du et al., 2000). In addition, although hypotonic buffers are generally sufficient to enable cytochrome c release from mitochondria, Smac/DIABLO release from similar organelle fractions has been reported to require the use of detergents (Du et al., 2000). Moreover, a recent study suggests that glucocorticoid‐induced Smac/DIABLO release from mitochondria proceeds in the absence of detectable cytochrome c export (Chauhan et al., 2001). Thus, it remains unclear whether the channel involved in modulating cytochrome c release also regulates Smac/DIABLO efflux.
It is well established that Bcl‐2 plays a critical role in regulating apoptosis by blocking efflux of cytochrome c from the mitochondrial intermembrane space (Kluck et al., 1997; Yang et al., 1997). To explore whether mitochondrial Smac/DIABLO release during apoptosis was similarly Bcl‐2 sensitive, we used a well‐characterized CEM cell line stably transfected with a Bcl‐2 expression plasmid (CEM.Bcl‐2), versus empty vector‐transfected control cells (CEM.vector). These cell lines were compared in terms of their response to a panel of pro‐apoptotic agents (Figure 4). Exposure of CEM.vector cells to a variety of stimuli, including staurosporine, actinomycin D and UVB‐irradiation, readily provoked morphological changes indicative of apoptosis in these cells (data not shown). CEM.Bcl‐2 cells were largely protected from apoptosis under the same conditions, exhibiting a morphology similar to untreated cells (data not shown). In response to these pro‐apoptotic agents, CEM.vector cells released substantial amounts of cytochrome c and Smac/DIABLO into the cytosol. In contrast, CEM.Bcl‐2 cells exhibited minimal accumulation of cytosolic cytochrome c or Smac/DIABLO under the same conditions (Figure 4). Comparison of the corresponding pellet fractions confirmed that loss of mitochondrial cytochrome c, or Smac/DIABLO, was substantially attenuated in CEM.Bcl‐2 cells (Figure 4).
Bcl‐2‐regulated cytochrome c and Smac/DIABLO efflux occur with similar kinetics
To investigate whether Smac/DIABLO and cytochrome c release generally occur within the same timeframe, we treated cells with UVB radiation or actinomycin D, and compared the kinetics of cytochrome c and Smac/DIABLO export in control versus Bcl‐2‐overexpressing cells (Figure 5).
As illustrated in Figure 5B, cytosolic fractions prepared from UVB‐treated CEM.vector cells had accumulated substantial amounts of cytochrome c within 3 h of treatment. Similarly, cytosolic fractions from actinomycin D‐treated vector transfectants contained substantial levels of cytochrome c within 7 h (Figure 5D). However, in response to both agents, the release of cytochrome c was potently blocked in the Bcl‐2 transfectants (Figure 5B and D). Probing the same fractions for Smac/DIABLO revealed that the latter accumulated in the CEM.vector cytosols over a similar timescale to cytochrome c and that its release was similarly blocked by Bcl‐2 (Figure 5B and D). Cells treated with the DNA‐damaging agent daunorubicin also exhibited similar kinetics of cytosolic cytochrome c and Smac/DIABLO accumulation (data not shown).
Death receptor‐initiated mitochondrial release of cytochrome c and Smac/DIABLO is caspase dependent
As discussed above, several lines of evidence suggest that the pore involved in mediating Smac/DIABLO release from mitochondria may be distinct from the cytochrome c‐releasing channel (Du et al., 2000; Chauhan et al., 2001; Martinou and Green, 2001). However, thus far, we have observed that Smac/DIABLO and cytochrome c efflux occur with similar kinetics, and that both events are repressed by Bcl‐2. This suggests that release of the two molecules may be regulated by the same mitochondrial pore (Figures 4 and 5).
With the important exception of caspase‐8‐catalysed Bid cleavage associated with death receptor engagement, mitochondrial cytochrome c release has been reported to be caspase independent in most cases (Bossy‐Wetzel et al., 1998; Li et al., 1998; Luo et al., 1998; Scaffidi et al., 1998; Zhuang and Cohen, 1998; Gross et al., 1999; Goldstein et al., 2000; Waterhouse et al., 2001). To explore whether the channel responsible for regulating Smac/DIABLO release was similarly caspase independent, we pre‐treated Jurkat cells with the pan‐caspase inhibitor, z‐VAD‐fmk, before stimulating them with a panel of pro‐apoptotic agents (Figure 6).
Initially, we examined Smac/DIABLO release in anti‐Fas‐treated cells, because it has been established that cytochrome c release is impaired by caspase inhibition in this cell death model (Bossy‐Wetzel and Green, 1999). As expected, anti‐Fas‐treated cells readily underwent apoptosis, which was substantially attenuated by pre‐incubation with z‐VAD‐fmk (data not shown). Analysis of subcellular fractions of these cells revealed that cytochrome c accumulation within the cytosol (and its loss from mitochondria) was potently repressed by z‐VAD‐fmk (Figure 6A). This is probably due to the requirement for caspase‐mediated cleavage of Bid (tBid), or a similar factor, to modulate cytochrome c release in this context (Li et al., 1998; Luo et al., 1998; Gross et al., 1999). Fas‐mediated Smac/DIABLO release from mitochondria was also substantially attenuated by pre‐treatment of the cells with z‐VAD‐fmk, suggesting a similar requirement for a caspase‐cleaved factor for Smac/DIABLO release in Fas‐induced death (Figure 6A). Thus, during death receptor‐induced apoptosis, active caspases are required to fuel Smac/DIABLO release.
Cell stress‐associated Smac/DIABLO efflux from mitochondria requires active caspases
We examined the ability of z‐VAD‐fmk to modulate Smac/DIABLO release in response to types of stimuli established to provoke caspase‐independent cytochrome c translocation (Kluck et al., 1997; Bossy‐Wetzel et al., 1998; Zhuang and Cohen, 1998; Chen et al., 2000). As anticipated, cells pre‐treated with z‐VAD‐fmk were protected from apoptosis induced by cytotoxic drugs and UVB‐irradiation, as judged by the paucity of apoptotic nuclei in these samples (Figure 6B), the absence of active caspase‐9 within the cytosols of the same cells or by the cleavage of the caspase‐3 substrate gelsolin (Figure 6C). As previously reported, the accumulation of cytochrome c within the cytosols of cells undergoing apoptosis in response to cytotoxic drugs and UV‐irradiation was largely insensitive to caspase inhibition (Figure 6C; Kluck et al., 1997; Bossy‐Wetzel et al., 1998; Zhuang and Cohen, 1998; Chen et al., 2000). However, in sharp contrast, the accumulation of Smac/DIABLO within the same cytosolic fractions was potently repressed in z‐VAD‐fmk‐treated cells, strongly suggesting that caspases were required to mediate Smac/DIABLO release in response to these pro‐apoptotic stimuli (Figure 6C).
To examine further the role of caspases in modulating Smac/DIABLO release from mitochondria, we also performed timecourse experiments (Figure 6D and E). Jurkat cells, pre‐incubated with z‐VAD‐fmk, were treated with actinomycin D and subcellular fractions were generated at the indicated timepoints (Figure 6E). In agreement with the endpoint experiment (Figure 6B and C), whereas cytosolic cytochrome c accumulation was largely uninhibited by caspase inhibition, z‐VAD‐fmk pre‐treated cells exhibited reduced levels of cytosolic Smac/DIABLO at all timepoints examined (Figure 6E). Similar results were obtained for cells induced to undergo apoptosis in response to UV‐irradiation (data not shown).
Immunolocalization of Smac/DIABLO and cytochrome c reveals divergent release patterns upon caspase inhibition
To confirm that caspase inhibition could uncouple cytochrome c and Smac/DIABLO release from mitochondria, we also performed immunolocalization experiments in HeLa cells. Figure 7A–C illustrates that cytochrome c and Smac/DIABLO co‐localized to mitochondria in viable (non‐apoptotic) cells, as expected. However, when cells were pre‐treated with z‐VAD‐fmk and induced to undergo apoptosis by exposure to UV radiation or actinomycin D, numerous cells could be found containing mitochondria that had clearly released cytochrome c but not Smac/DIABLO (Figure 7). In the latter cells, cytochrome c had assumed a diffuse staining pattern within the cell and could also be seen within the nucleus (Figure 7D–L). In sharp contrast, Smac/DIABLO was clearly retained within mitochondria and exhibited a punctate distribution similar to that seen in surrounding cells where cytochrome c release had not yet occurred (Figure 7D–L). Where z‐VAD‐fmk was not added to the culture medium, cells that had released cytochrome c from mitochondria also released Smac/DIABLO and rapidly progressed to a rounded and blebbed apoptotic phenotype. In the latter situation, both proteins exhibited a diffuse extramitochondrial distribution within the cell (Figure 8).
Thus, caspase inhibition attenuates the cytosolic accumulation of Smac/DIABLO but not cytochrome c. Taken together, these data suggest that whereas the pore responsible for mediating cytochrome c export is largely caspase‐independent, active caspases are required to enable Smac/DIABLO efflux from mitochondria provoked by the same stimuli.
Here we report that Smac/DIABLO release from mitochondria is a general feature of apoptosis. In cells stimulated to die in response to diverse pro‐apoptotic agents, including death receptor ligation, cytotoxic drugs and DNA‐damaging agents, Smac/DIABLO accumulation within the cytosol was readily detected (Figures 2 and 3). This coincided with a concomitant loss of Smac/DIABLO from mitochondria. Using a cell line that constitutively overexpresses Bcl‐2, we have also demonstrated that Bcl‐2 blocks apoptosis‐associated release of Smac/DIABLO from mitochondria (Figures 4 and 5). These data expand the already established anti‐apoptotic roles of Bcl‐2. Whereas cytochrome c release lies upstream of caspase activation in many forms of stress‐induced apoptosis, data presented herein suggest that active caspases are required to enable Smac/DIABLO release (Figures 6, 7 and 8). This would suggest a model whereby the ability of Bcl‐2 to inhibit Smac/DIABLO release from mitochondria may not relate to its ability to regulate a Smac/DIABLO‐exporting channel directly (Figure 9). Rather, by regulating cytochrome c translocation into the cytosol and preventing apoptosome assembly, it is conceivable that Bcl‐2 indirectly regulates Smac/DIABLO release by blocking caspase activation via the apoptosome (Figure 9).
Clearly, one implication of this observation is that the nature of the Smac/DIABLO‐escape channel may differ from that of the cytochrome c‐releasing pore. Given the significant difference in size between cytochrome c (∼12 kDa) and of Smac/DIABLO dimers (∼100 kDa), one possibility is that the latter molecule is simply too large to escape from the cytochrome c‐conducting channel (Martinou and Green, 2001). This is consistent with the observation that extraction of Smac/DIABLO from mitochondria requires the use of detergents, whereas cytochrome c extraction does not (Du et al., 2000). Our data further support the possibility that the mechanisms governing cytochrome c and Smac/DIABLO release differ. In contrast to cytochrome c, Smac/DIABLO release from mitochondria appears to require caspase‐catalysed attack of a mitochondrial component (Figure 9).
Within this context, it is interesting to note data concerning the role of caspases in promoting the loss of mitochondrial transmembrane potential (Bossy‐Wetzel et al., 1998; Marzo et al., 1998; Waterhouse et al., 2001). Apoptosis‐associated cytochrome c release appears to involve limited permeabilization of the outer mitochondrial membrane and maintenance of transmembrane potential. However, cytochrome c/apoptosome‐mediated caspase activation appears to result in swelling of the mitochondrial matrix and rupture of the inner and outer mitochondrial membranes (Martinou and Green, 2001; Zamzami and Kroemer, 2001). Interestingly, it has been established that the megapore spanning the inner and outer mitochondrial membranes created by permeability transition pore opening enables the efflux of molecules larger than cytochrome c (Marzo et al., 1998; Kroemer, 1999; Patterson et al., 2000). Thus, caspase‐dependent mitochondrial attack, which may result in permeability transition pore opening or generalized mitochondrial destruction, could facilitate Smac/DIABLO release from mitochondria downstream of cytochrome c efflux (Figure 9). Clearly, further studies will be required to evaluate this hypothesis.
Materials and methods
Anti‐cytochrome c, anti Bcl‐2, anti‐caspase‐3 and anti‐gelsolin antibodies were purchased from BD PharMingen (UK). Antibodies selectively recognizing active caspase‐9 (generated by cleavage at Asp330) were purchased from New England Biolabs (UK). Anti‐actin antibodies were from ICN (Aurora, OH). z‐VAD‐fmk was purchased from Bachem (UK). Human multi‐tissue lysates were purchased from Clontech (UK). The anti‐Fas agonistic antibody (clone CH‐11) was obtained from Kamiya Biomedical Co. Unless otherwise indicated, all other reagents were purchased from Sigma (Ireland) Ltd.
Generation and affinity purification of Smac/DIABLO‐specific antisera
BL21 bacteria, harbouring a plasmid encoding the mature form of human Smac/DIABLO, which lacks the mitochondrial targeting sequence (a kind gift of Dr Xiaodong Wang, University of Texas Medical Center), were induced to express recombinant His6‐Smac by the addition of 1 mM IPTG to culture media. The pellets from 1 l of bacterial culture were lysed by sonication in lysis buffer [20 mM Tris–Cl pH 7.9, 500 mM NaCl, 5 mM imidazole, 0.1% NP‐40, 100 μM phenymethylsulfonyl fluoride (PMSF), 10 μg/ml leupeptin, 2 μg/ml aprotinin]. Recombinant polyhistidine‐tagged Smac was captured from the clarified lysate by incubation with Ni‐agarose beads (Qiagen, UK) followed by extensive washing in wash buffer (20 mM Tris–HCl pH 7.9, 500 mM NaCl, 60 mM imidazole). Affinity‐purified Smac/DIABLO was eluted by incubation in wash buffer supplemented with 500 mM imidazole and the resultant soluble protein was washed extensively in phosphate‐buffered saline (PBS) pH 7.2 in a microfiltration unit (Vivascience Ltd, UK). A rabbit polyclonal anti‐Smac/DIABLO antibody was generated by immunizing rabbits with purified recombinant Smac/DIABLO protein. To purify the antibody, the crude antiserum was pre‐cleared with Ni‐agarose beads for 6 h at 4°C. Smac/DIABLO‐specific antibodies were subsequently captured for 12 h using bead‐immobilized recombinant Smac/DIABLO protein as an affinity matrix. Bead‐captured antibodies were washed extensively in PBS pH 7.2, then acid eluted into 100 mM glycine pH 3.0, followed by neutralization with one‐tenth of a volume of 1 M Tris, pH 8.0. Prior to use, purified antibodies were washed extensively in PBS and concentrated ∼8‐fold in a microfiltration unit.
Determination of Smac/DIABLO expression patterns
To determine the tissue distribution of Smac/DIABLO, a panel of human tissue lysates (Clontech) were subjected to standard 12% SDS–PAGE, followed by immunoblotting with Smac/DIABLO antibodies. To assess Smac/DIABLO expression levels in human tumour cells, a variety of cell lines were lysed at a density of 107 cells/ml in standard SDS–PAGE loading buffer. Samples (50 μg) were subjected to 12% SDS–PAGE and immunoblotted with anti‐Smac/DIABLO antibodies.
Induction and assessment of apoptosis
To induce apoptosis, cells were seeded at a final density of 2 × 106/ml in the presence of pro‐apoptotic agents at concentrations indicated in the Figure legends. Alternatively, cells were exposed to UVB‐irradiation for 90 s on a Bio‐Rad UV transilluminator 2000 (Bio‐Rad, UK). Where indicated, cells were pre‐incubated in medium containing 50 μM z‐VAD‐fmk for 2 h prior to the induction of apoptosis.
To assess the extent of apoptosis, cells (106) were harvested by centrifugation at 800 g, for 10 min. Cell pellets were resuspended in 100 μl of ice‐cold fixative solution (4% paraformaldehyde in PBS pH 7.2) supplemented with 10 μM Hoechst. The percentage of cells exhibiting apoptotic nuclei, as judged by chromatin condensation and nuclear fragmentation, was then determined by fluorescence microscopy.
Following induction of apoptosis, cytosolic and pellet (mitochondrial) fractions were generated using a digitonin‐based subcellular fractionation technique, essentially as described previously (Ekert et al., 2001; Waterhouse et al., 2001). Briefly, 107 cells were harvested by centrifugation at 800 g, washed in PBS pH 7.2, and re‐pelletted. Cells were digitonin‐permeabilized for 5 min on ice at a density of 3 × 107/ml in cytosolic extraction buffer (250 mM sucrose, 70 mM KCl, 137 mM NaCl, 4.3 mM Na2HPO4, 1.4 mM KH2PO4 pH 7.2, 100 μM PMSF, 10 μg/ml leupeptin, 2 μg/ml aprotinin, containing 200 μg/ml digitonin). Plasma membrane permeabilization of cells was confirmed by staining in a 0.2% trypan blue solution. Cells were then centrifuged at 1000 g for 5 min at 4°C. The supernatants (cytosolic fractions) were saved and the pellets solubilized in the same volume of mitochondrial lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 2 mM EDTA, 2 mM EGTA, 0.2 % Triton X‐100, 0.3% NP‐40, 100 μM PMSF, 10 μg/ml leupeptin, 2 μg/ml aprotinin), followed by pelletting at 10 000 g for 10 min at 4°C. For the detection of Smac/DIABLO or cytochrome c, equal volumes (28 μl) of cytosolic and pellet fractions were supplemented with 5× SDS–PAGE loading buffer, subjected to standard 12% SDS–PAGE and transferred to nitrocellulose membranes (Schleicher and Schuell, UK). Blots were probed with anti‐cytochrome c or anti‐Smac/DIABLO antibodies and detected with horseradish peroxidase‐coupled secondary antisera using the Supersignal West Pico or Supersignal West Dura chemiluminescence reagents (Pierce, UK).
Immunostaining of cytochrome c and Smac/DIABLO
HeLa cells were seeded on coverslips at a density of 5 × 104 cells/well in 6‐well tissue culture plates 48 h prior to treatment. Prior to induction of apoptosis, cells were pre‐incubated in control media or in the presence of 100 μM z‐VAD‐fmk for 2 h. Cells were then UVB‐irradiated for 7 min, or treated with 10 μM actinomycin D, to induce apoptotic changes. Eight hours later, cells were washed three times in PBS pH 7.2, followed by fixation in 3% paraformaldehyde/PBS for 10 min. Fixed cells were then washed three times in PBS and permeabilized in 0.15% Triton X‐100 in PBS for 15 min. Cells were blocked for 30 min in 2% bovine serum albumin (BSA)/PBS and probed with a 1:100 dilution of anti‐cytochrome c (clone 6H2.B4, BD PharMingen) and anti‐Smac/DIABLO antibodies (this study) for 1 h at room temperature. After incubation with primary antibodies, cells were washed three times (10 min per wash) in 2% BSA/PBS with gentle rocking, followed by probing for 45 min with a 1:250 dilution of fluorescein‐conjugated anti‐mouse and rhodamine‐conjugated anti‐rabbit antibodies (Molecular Probes, The Netherlands). Immuno stained cells were then washed three times in PBS and mounted in Slowfade Light Antifade mounting medium (Molecular Probes). Confocal images were obtained using a Bio‐Rad confocal microscope.
We thank Dr Xiaodong Wang for the provision of the Smac bacterial expression plasmid. E.M.C. was supported by a Health Research Board of Ireland Post‐Doctoral Fellowship. This work was funded by a Wellcome Trust Senior Fellowship Award in Biomedical Science (047580) and EU grant (QLG1‐1999‐00739) to S.J.M. We also thank Science Foundation Ireland for support of ongoing work in our laboratory.
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