DNA from Kinetoplastida contains the unusual modified base β‐d‐glucosyl(hydroxymethyl)uracil, called J. Base J is found predominantly in repetitive DNA and correlates with epigenetic silencing of telomeric variant surface glycoprotein genes in Trypanosoma brucei. We have now identified a protein in nuclear extracts of bloodstream stage T.brucei that binds specifically to J‐containing duplex DNA. J‐specific DNA binding was also observed with extracts from the kinetoplastids Crithidia fasciculata and Leishmania tarentolae. We purified the 90 kDa C.fasciculata J‐binding protein 50 000‐fold and cloned the corresponding gene from C.fasciculata, T.brucei and L.tarentolae. Recombinant proteins expressed in Escherichia coli demonstrated J‐specific DNA binding. The J‐binding proteins show 43–63% identity and are unlike any known protein. The discovery of a J‐binding protein suggests that J, like methylated cytosine in higher eukaryotes, functions via a protein intermediate.
DNA modification is a feature of many prokaryotic and eukaryotic genomes. Cytosine methylation, a covalent modification of DNA at CpG dinucleotides, is the most prevalent and best studied DNA modification in eukaryotes. It is involved in gene silencing at the level of transcription initiation and elongation, it can act as an imprinting signal and it has been suggested to be required for the suppression of intragenomic parasitic sequences such as retroviruses, Alu elements and transposons (reviewed in Jaenisch, 1997; Kass et al., 1997; Yoder et al., 1997; Colot and Rossignol, 1999).
Although kinetoplastid flagellates such as the parasite Trypanosoma brucei contain no methylated cytosine, they do have β‐d‐glucosyl(hydroxymethyl)uracil (called J), a modified base that replaces a fraction of thymine in their DNA (Gommers‐Ampt et al., 1993; Borst and Van Leeuwen, 1997; Van Leeuwen et al., 1998c). J is made in two steps, thymine first being converted to 5‐(hydroxymethyl)uracil, which is subsequently glucosylated (Van Leeuwen et al., 1998b). Whilst J is enriched in the telomeric repeats of all Kinetoplastida, in T.brucei it is also found in other repetitive sequences and in the telomeric variant surface glycoprotein (VSG) gene expression sites involved in antigenic variation (Van Leeuwen et al., 1996, 1997). Here J is present in genes in silent sites but not in the active site (Bernards et al., 1984; Pays et al., 1984; Van Leeuwen et al., 1997). Recent experiments have shown that a decrease in the level of J in the genome can increase transcription near a silent VSG promoter (Van Leeuwen et al., 1998b). These data support the hypothesis that J is involved in the transcriptional repression of silent VSG gene expression sites, perhaps in a manner akin to methylated cytosine in higher eukaryotes.
J is conserved across the Kinetoplastida (Van Leeuwen et al., 1998c), most of which do not undergo antigenic variation. In T.brucei, J is abundant in and around non‐transcribed repetitive sequences, and clustering of J in other Kinetoplastida has also been observed. It is therefore possible that the primary function of J is not in regulating transcription of specific genes, but rather in suppressing recombination between repetitive DNA elements to maintain chromosome stability. Indeed, we have found recently that DNA rearrangements sometimes observed with VSG gene expression site switching in T.brucei are reduced when the level of J in the genome is increased (Van Leeuwen et al., 1998b). To accommodate the different putative roles of J in transcriptional repression and maintenance of genome integrity, we have proposed that J results in the formation or stabilization of a condensed chromatin structure (Van Leeuwen et al., 1998b,c). This is likely to occur through the recruitment of specific proteins that modify chromatin, as is the case for methylated cytosine in mammals, where proteins such as MeCP2 bind and mediate transcriptional repression (Meehan et al., 1989; Lewis et al., 1992; Hendrich and Bird, 1998). Another example is silencing in the yeast Saccharomyces cerevisiae (reviewed in Grunstein, 1997; Sherman and Pillus, 1997). Here a complex of silencing proteins is recruited to telomeric repeats and to the mating type loci.
Here we describe the identification of J‐binding proteins from T.brucei and the related kinetoplastid parasites Crithidia fasciculata and Leishmania tarentolae that specifically bind double‐stranded DNA containing J. Following purification of the C.fasciculata J‐binding protein, we identified the corresponding gene and then cloned genes for similar proteins from T.brucei and L.tarentolae. The J‐binding protein is conserved between these three species, but shows no significant homology with any other known protein.
A JBP activity in trypanosome nuclear extract
We detected J‐specific DNA‐binding proteins in nuclear extracts of T.brucei bloodstream form using a bandshift assay with chemically synthesized DNA oligonucleotides of different sequences that contain base J (Van Leeuwen et al., 1996, 1997, 1998a; see Table I): 4× telomere repeats (tel‐J) and a VSG gene sequence containing a PvuII restriction site (vsg‐J). To control for non‐specific binding, probes of the same sequence but with Thy in place of J were used. J‐specific binding was observed only with double‐stranded versions of the probes. Figure 1A shows that upon addition of extract to the reaction with probe tel‐J, several complexes were formed. The slowest migrating complex is likely to be J‐specific as it was absent from the reaction with probe tel, the J‐free version of tel‐J. This was verified by competition experiments (Figure 1A; summarized in Table I). A 27‐fold molar excess of unlabelled double‐stranded tel‐J abolished formation of the upper complex, as did double‐stranded vsg‐J; vsg‐J did not compete complex formation as effectively as tel‐J, but this was difficult to quantify due to the low abundance of the J‐specific complex. The J‐free versions of these DNAs showed no significant competition, neither did Escherichia coli or calf thymus double‐stranded DNA. The J‐specific complex was observed when vsg‐J was used as probe, and the effect of different competitor DNAs on complex formation with vsg‐J was the same as that seen with tel‐J (data not shown). None of the additional non‐J‐specific complexes observed in the bandshift assay were investigated any further.
Crithidia also have base J, so we repeated the binding studies with nuclear extract prepared from C.fasciculata (Figure 1B). A J‐specific bandshift with probe tel‐J was again observed, although compared with T.brucei the C.fasciculata complex migrated much further in the gel, and it was more abundant (see also Figure 2A) and less sensitive to the assay conditions. Complex formation was competed effectively only by J‐containing DNAs. In this case, vsg‐J competed ∼5‐fold less than tel‐J. Binding reactions with C.fasciculata extract and probe vsg‐J also produced the J‐specific complex (data not shown). The slower migrating non‐specific complex seen with tel‐J did not form, however, consistent with the observation that unlabelled vsg‐J could not compete this complex (Figure 1B). We found that nuclear extract derived from L.tarentolae promastigotes also contains a J‐specific binding activity (Figure 2A). In this case, the bandshift resembles the C.fasciculata J‐specific complex. Procyclic stage T.brucei do not contain J, so we tested nuclear extracts made from procyclic trypanosomes for J‐binding activity. Using probe tel‐J, procyclic extract gave non‐specific complexes similar to bloodstream form extract, but the J‐specific complex was absent (Figure 2A). Thus, of those cells tested, there is a firm correlation between the presence of J in DNA and the observation of J‐binding activity in nuclear extract. To determine the nature of the J‐binding activity, T.brucei bloodstream form and C.fasciculata nuclear extracts were pre‐treated with proteinase K before addition to the binding reaction (data not shown). In both cases, this abolished complex formation, which was restored when phenylmethylsulfonyl fluoride (PMSF) was included in the proteinase K treatment step, indicating that the J‐binding activity has a protein component (referred to as JBP).
J is made in two steps: first a thymine in DNA is converted to 5‐(hydroxymethyl)uracil, which in turn is glucosylated to form J (Van Leeuwen et al., 1998b). To determine whether the JBP found in trypanosome nuclear extract may have some affinity for the intermediate in J synthesis, we tested whether or not DNA containing 5‐(hydroxymethyl)uracil could inhibit JBP binding in the bandshift assay. First of all we found that bacteriophage φe DNA, in which all thymines are replaced by 5‐(hydroxymethyl)uracil, did not compete JBP binding even at 860‐fold molar excess (data not shown; Table I). Furthermore, oligo duplex tel‐V, which is the same as tel‐J but has 5‐(hydroxymethyl)uracil in place of J, had no effect on JBP complex formation (Figure 2B). We also tested pdJ and the nucleotide sugars UDPG and GDPG, none of which were found to compete the JBP complex (data not shown; Table I). These results suggest that JBP recognizes base J in DNA.
Purification of the C.fasciculata JBP
We concentrated on purifying the JBP from C.fasciculata as this seemed the most practical way to generate sufficient material for later analysis. Fractionation was monitored for JBP by bandshift assay. Precipitation of crude nuclear extract with 20% polyethylene glycol (PEG) left most of the JBP in the supernatant and fractionated away the non‐specific DNA‐binding activity detected by probe tel‐J (Figure 3A, lanes NE and P20 sup). Although the P20 sup fraction still contained a complex mixture of proteins as judged by SDS–PAGE and silver staining (Figure 3B), 80–85% of the bulk protein was removed routinely in this step. The P20 sup fraction was then passed over DNA–cellulose to remove any contaminating DNA‐binding proteins and nucleases. JBP was recovered without loss in the flow‐through (Figure 3A, lane DNA FT) and the total amount of protein in this fraction was unchanged. The final step in the purification scheme involved specific binding of JBP to J‐containing DNA, using Dynabeads coated with streptavidin to immobilize biotinylated J‐containing oligo duplex and retrieve JBP from the DNA FT fraction. We found that when duplex vsg‐J containing a single J was used as ligand during affinity chromatography, JBP activity was absent from the unbound fraction and reappeared following elution from the beads with high‐salt buffer (Figure 3A, lanes vsg‐J FT and E). JBP binding to the vsg‐J–beads approached 100%, and we were able routinely to recover 50–90% JBP activity in the eluate. To control for non‐specific binding to the oligo–beads, we also performed affinity chromatography using duplex vsg in place of vsg‐J. This time all of the JBP activity remained in the unbound fraction, with nothing in the high‐salt eluate (Figure 3A, lanes vsg FT and E). SDS–PAGE and silver staining of the eluate from the vsg‐J affinity chromatography identified a single protein of ∼90 kDa, which was absent from the control (Figure 3B, lanes vsg‐J E and vsg E, respectively). With protein from a larger scale purification, we were able to detect the 90 kDa JBP after staining with Coomassie Blue (data not shown). By comparison with protein molecular mass markers, we estimate that the yield of JBP is ∼1 μg per 2.5 × 1011 cells, an enrichment of ∼50 000‐fold.
Cloning the C.fasciculata, T.brucei and L.tarentolae JBP genes
Purified C.fasciculata JBP was analysed by SDS–PAGE, stained with silver, in‐gel digested with trypsin and subjected to mass spectrometric sequencing. The peptide sequences obtained were then used to design degenerate DNA oligonucleotides to amplify the corresponding cDNA, which was used to clone the JBP gene by screening a size‐fractionated C.fasciculata genomic library. Using this approach, we isolated a C.fasciculata gene with a predicted open reading frame of 811 amino acids, resulting in a protein with a molecular mass of 90 kDa (Figure 4). All seven of the peptide sequences obtained from the purified protein were found in the predicted JBP sequence.
To clone the T.brucei and L.tarentolae JBP genes by homology, we used a probe spanning amino acids 88–702 of the C.fasciculata JBP gene. Screening size‐fractionated genomic libraries yielded a T.brucei gene and an L.tarentolae gene homologous to the C.fasciculata JBP gene (Figure 4). No other genes homologous to the C.fasciculata JBP gene were detected in these libraries. The T.brucei and L.tarentolae JBPs are predicted to contain 839 and 827 amino acids, respectively, with a molecular mass of 95 and 93 kDa, respectively. Alignment of the predicted protein sequences reveals that the C.fasciculata and L.tarentolae JBPs are more closely related to each other (64% identity) than they are to T.brucei (both 43% identity). However, all three proteins show significant blocks of similarity at the levels of amino acid sequence and predicted secondary structure, particularly in the N‐terminal half. A computer‐assisted search of the available databases revealed JBP to be unlike any other known protein.
Recombinant C.fasciculata and T.brucei JBPs bind specifically to J‐containing DNA
To validate that the gene we had cloned really encodes the JBP, we produced full‐length C.fasciculata JBP in E.coli using the pET expression system. Although almost 90% of the recombinant JBP (rJBP) was insoluble in the lysate pellet fraction, sufficient quantities of soluble rJBP could be recovered in the supernatant. The rJBP was purified by metal affinity chromatography by virtue of the N‐terminal His6 tag engineered on to the protein (Figure 5A, left panel). J‐binding activity was then monitored by the bandshift assay. Figure 5A shows that rJBP formed a complex with probe tel‐J, which migrated similarly to that observed with C.fasciculata nuclear extract. Furthermore, complex formation was competed only by J‐containing DNA. Lysates from uninduced E.coli or from E.coli carrying the pET vector alone showed no J‐binding activity (data not shown). These results prove that the gene we cloned is indeed the JBP gene.
We also expressed His‐tagged recombinant T.brucei JBP in E.coli. In this case, expression was poor, resulting in amounts of soluble rJBP that could only be detected by immunostaining with antibodies against the N‐terminal His6 tag (Figure 5B, left panel). Nevertheless, T.brucei rJBP was found to bind specifically to probe tel‐J in the bandshift assay (Figure 5B, centre panel). Several complexes were formed, mostly migrating to a position in the gel similar to that seen for C.fasciculata JBP. This was unexpected since the J‐specific complex we had characterized from T.brucei nuclear extract migrates much more slowly in the gel. The difference is unlikely to be due to shorter forms of rJBP, as no significant degradation was observed following the DNA‐binding reaction (Figure 5B, left panel). To confirm these results, we used a UV cross‐linking assay to monitor J‐specific binding and to detect the protein bound to J‐containing DNA (Figure 5B, right panel). With T.brucei nuclear extract, a protein of ∼110 kDa was cross‐linked specifically to probe tel‐J. After correction for the mass of the attached oligo duplex (end‐labelled with 32P), we estimate the size of the J‐binding‐protein in nuclear extract to be ∼97 kDa. This closely matches the predicted 95 kDa encoded by the T.brucei JBP gene we have cloned. We were also able to detect specific cross‐linking of probe tel‐J to the recombinant JBP (Figure 5B, left panel), confirming the J‐binding activity of this protein.
We have identified a J‐binding protein in nuclear extracts of T.brucei bloodstream form and the related kinetoplastids C.fasciculata and L.tarentolae. Purification of the C.fasciculata JBP allowed the cloning of the corresponding gene and its homologues from T.brucei and L.tarentolae. JBP binds only to double‐stranded DNA containing the modified base J. DNA of the same nucleotide sequence but containing Thy instead of J is not recognized by JBP. In bloodstream form trypanosomes, J is found in such a variety of different sequence contexts that assigning a modification consensus site has not been possible (Van Leeuwen et al., 1997). We tested two different sequences as substrate for JBP: the telomere repeat and a segment of a VSG gene. A large proportion of J is found in telomeric repeats and its location has been mapped previously (Van Leeuwen et al., 1996), whilst the presence of J in the VSG gene has been inferred from a blocked restriction site (Van Leeuwen et al., 1997). Both J‐containing sequences bind the T.brucei and C.fasciculata JBPs, even though VSG genes are not found in C.fasciculata. By using two very different DNAs as probes for J binding, we focused on proteins that bind to J rather than to a particular nucleotide sequence. This was especially important with the telomere repeat, as proteins that bind to this sequence have already been identified in T.brucei (Eid and Sollner‐Webb, 1995, 1997; Field and Field, 1996), C.fasciculata (Tzfati et al., 1992) and Leptomonas (Field and Field, 1996). It is possible that some of the non‐J‐specific complexes we observed with the telomere repeat probe were due to these proteins or their homologues. None of these complexes appeared to be affected by the presence of J, so we did not investigate them any further. Although JBP can recognize base J in different sequences, it may do so with variable affinity. J‐containing telomeric repeats compete JBP binding more effectively than J‐containing VSG sequence, and the JBP–telomere repeat complex is resistant to high salt. This may be due to the differences in length, amount of J or sequence between the two duplex DNAs. Further experiments with a greater variety of J‐containing sequences and purified recombinant protein, together with the determination of the crystal structure of JBP complexed to DNA, should resolve this issue. What is clear is that JBP does not bind the free nucleotide J, suggesting that J in the context of DNA is the target. This is reinforced by the fact that the glucose‐free intermediate in J synthesis, 5‐(hydroxymethyl)uracil in DNA, is also not bound by JBP.
If J has evolved as a tag on DNA to direct the binding of a specific protein, those organisms that contain J should also possess a JBP. We found this to be true for the three species we tested: T.brucei, C.fasciculata and L.tarentolae. In addition, no JBP activity was detected in procyclic form T.brucei, a stage in the life cycle where J is absent. Northern blot analysis indicates that the JBP mRNA is absent from procyclic T.brucei (data not shown). The J‐specific complex we observed in the bandshift assay with C.fasciculata nuclear extract is most likely to be due to the binding of JBP alone to J‐modified DNA, since recombinant C.fasciculata JBP expressed in E.coli produced the same complex. Although we cloned the JBP gene from T.brucei by virtue of its homology to the C.fasciculata gene, we believe that it encodes the J‐binding activity we found in T.brucei nuclear extract for the following reasons. First, the encoded protein expressed in E.coli shows J‐specific DNA binding in both bandshift and UV cross‐linking assays. Secondly, the size of the J‐binding protein in nuclear extract, as detected by UV cross‐linking, corresponds to that predicted from the gene. Thirdly, the J‐specific, UV‐cross‐linked protein is absent from the nuclear extract of a JBP gene knockout cell line obtained very recently (M.Cross and P.Borst, unpublished results). Why the J‐specific complex formed by endogenous JBP in nuclear extract differs from that formed by recombinant JBP expressed in E.coli, as judged by native gel electrophoresis, is unclear. It is possible that the T.brucei JBP has a tendency to aggregate and form multimers or bind other proteins, and that this might be somewhat less for the His‐tagged protein than for the native JBP.
The amount of C.fasciculata JBP we were able to purify from nuclear extract was low. Given that we could recover >50% of the JBP present, as determined by bandshift assay, we estimate a yield of only 50 molecules of JBP per cell. As C.fasciculata contains ∼2.4 × 105 molecules of J per cell (Van Leeuwen et al., 1998c), it is likely that we have succeeded in purifying only a fraction of the actual amount of JBP present. No JBP activity could be detected in the cytoplasmic fraction of the cell extract, suggesting that leakage of JBP during the isolation of nuclei is not a major problem (data not shown). Instead it is possible that nuclear extraction strips away only a subpopulation of JBP. The greatest proportion of JBP is expected at the telomeres as this is where most of J is located. Since we found that JBP binds to the telomere repeat probe even at a high salt concentration, it is possible that most of the JBP is not recovered during cellular extraction. Additional stabilization of JBP by interactions with telomere‐binding proteins may also hinder extraction, similarly to what has been described in other organisms (Fang and Cech, 1995).
JBP is a novel protein and shows similarity throughout its sequence between T.brucei, C.fasciculata and L.tarentolae. Computer‐assisted domain searching of the PROSITE database predicts several interesting regions conserved in all three JBPs (Figure 4). Based upon the C.fasciculata JBP sequence, amino acids 253–276 show 80% similarity to the Myb DNA‐binding repeat signature (Biedenkapp et al., 1988). Furthermore, all three JBPs contain conserved putatively helical regions proximal to this motif, as would be expected for a derivative of this helix–turn–helix‐type DNA‐binding domain. The central region of JBP is rich in aliphatic residues and shows 75% similarity to the leucine zipper motif (Landschulz et al., 1999), and may therefore serve to recruit to J‐modified DNA other proteins that could have eluded the nuclear extraction procedure we used. In the C‐terminal half of JBP, amino acids 525–537 show 80% similarity to the EF‐hand calcium‐binding domain (Kawasaki and Kretsinger, 1995), and amino acids 544–556 contain the bipartite nuclear localization signal consensus sequence (Robbins et al., 1991). We have no experimental data as yet to suggest that calcium is required for the function of JBP.
Previously, we have investigated the effect on T.brucei of altering the level of J in the genome through the use of thymidine analogues (Van Leeuwen et al., 1998b). Lowering the J content caused a partial derepression of silent VSG gene expression site promoters. Raising the J content caused a reduction in the occurrence of chromosome breakage events that are sometimes associated with transcriptional switching between VSG gene expression sites. We speculated that these effects are mediated by the packaging of J‐containing DNA into a specialized chromatin structure via the recruitment for a protein factor. Our finding that base J directs the binding of a specific protein to DNA is consistent with this hypothesis. Little is known about chromatin structure in T.brucei, and it is a subject of debate with respect to VSG expression sites. Chromatin nuclease sensitivity assays to compare active and silent expression sites have given conflicting results (Greaves and Borst, 1987; Navarro and Cross, 1998). Recently, Navarro et al. (1999) showed that inactive expression site chromatin is accessible to bacteriophage T7 RNA polymerase. However, as the authors pointed out, this result does not rule out a role for chromatin modification in silencing expression sites. There are several well‐documented cases in eukaryotes of epigenetic silencing mediated by DNA‐binding proteins. Perhaps the closest analogy to base J is methylation of the 5‐position of cytosine in CpG dinucleotides, a covalent DNA modification that is involved in the mitotic inheritance of transcriptionally repressed chromatin states (reviewed in Kass et al., 1997). In vertebrates, differences in DNA methylation status are associated with gene imprinting, carcinogenesis and the suppression of mobile element activity (Jaenisch, 1997; Jones and Gonzalgo, 1997; Yoder et al., 1997). A conserved family of proteins has been described that specifically bind methyl‐CpG, and one of these, MeCP2, mediates transcriptional repression. Although there does not appear to be enough MeCP2 in the cell to occupy every methyl‐CpG, it has been shown recently that MeCP2 recruits a co‐repressor complex containing histone deacetylase activity (Jones et al., 1998; Nan et al., 1998). It now appears that methylated cytosine residues are important in guiding histone deacetylase to specific chromatin domains, and that the methylation‐dependent transcriptional silencing relies upon histone deacetylation. It remains to be seen if the modified base J, like methylated cytosine, acts as the signal to target chromatin for histone deacetylation (or other modification) and subsequent inactivity in trypanosomes. The identification of a J‐binding protein and the ability to produce large amounts of it in E.coli should allow us to address these questions.
Materials and methods
Cells and nuclear extracts
Trypanosoma brucei bloodstream form (clone 221a) and procyclic trypanosomes (strain 427) were grown as described (Van Leeuwen et al., 1997). Crithidia fasciculata were grown in 3.7% brain–heart infusion broth containing 20 μg of haemin/ml. Leishmania tarentolae (tarVIa) promastigotes were grown in SDM‐79 (Brun and Schoenenberger, 1979). Nuclear extracts were prepared according to Field and Field (1996), except that the high‐salt extracts were either dialysed against buffer H100 [25 mM HEPES–NaOH pH 7.9, 100 mM KCl, 5 mM MgCl2, 1 mM EDTA, 10% glycerol, 0.5 mM dithiothreitol (DTT)] or used directly in subsequent purification steps. The protein concentration of the nuclear extract was typically 2–4 mg/ml.
DNA oligonucleotides used in this study are listed in Table I. Oligos were end‐labelled with [γ‐32P]ATP and purified by exclusion chromatography. For double‐stranded DNA probes, the labelled oligo was annealed to its non‐labelled complementary strand. Annealing was controlled by electrophoretic analysis of the duplex DNAs. Double‐stranded oligo competitors were prepared in a similar manner except that both strands were non‐labelled. The E.coli and calf thymus DNA competitors were sonicated to fragments of ∼500 bp and annealed.
Binding reactions contained 150 fmol of probe mixed with 1 μg of nuclear extract (or the equivalent for purified fractions) in 25 mM HEPES–NaOH pH 7.9, 1 mM EDTA, 1 mM DTT, 10 μg of bovine serum albumin (BSA), 2 μg of poly(dI–dC)·poly(dI–dC) (Sigma) and 4 μg of α‐casein, in a final volume of 20 μl. KCl and MgCl2 concentrations were adjusted to 80 and 2.5 mM, respectively, for T.brucei extracts, and 100 and 5 mM, respectively, for both C.fasciculata and L.tarentolae extracts. If used, competitor DNAs were added to the reaction before the probe. Reactions were incubated at 30°C for 30 min. Complexes were resolved by electrophoresis at 150 V through a native 4.5% polyacrylamide (19:1) gel in 0.5× TBE and detected by autoradiography.
Purification of C.fasciculata JBP
The C.fasciculata JBP purification scheme was based upon the fractionation of J‐specific DNA‐binding activity. Crude nuclear extract was precipitated with 20% PEG 6000 for 60 min on ice. Under these conditions, JBP remains in solution. After centrifugation at 15 000 g for 10 min, the supernatant was recovered, diluted with two volumes of buffer H100 and dialysed against the same buffer, resulting in the PEG20 sup fraction. This was then passed over DNA cellulose (1:1 single‐stranded to double‐stranded calf thymus DNA; Pharmacia) equilibrated in buffer H100 at a flow rate of 14 ml/h, and the flow‐through collected (DNA FT fraction). JBP was then affinity purified from this fraction using a J‐containing DNA oligo. Briefly, 25 pmol of a biotinylated version of duplex oligo vsg‐J (see Table I) were coupled per milligram of Dynabeads–streptavidin (Dynal), according to the manufacturer. The oligo–beads were resuspended in buffer M (as buffer H100 but containing no MgCl2) and added to the DNA FT fraction at a concentration of 14 μg beads/μg of protein. EDTA was added to 5 mM, followed by an incubation on ice for 30 min. The beads were then washed three times for 20 min each with buffer M containing 0.32 mg/ml poly(dI–dC)·poly(dI–dC), the volume used being equal to the starting volume of the DNA FT fraction. JBP was then eluted from the beads by incubation on ice for 15 min with one volume of buffer M containing 500 mM KCl. The affinity‐purified fractions were precipitated with 10% trichloroacetic acid (TCA) and analysed by SDS–PAGE and silver staining (Bio‐Rad Silver Plus). Oligo–beads were regenerated for further use by incubation with 2 M KCl for 10 min and washing with buffer M.
Purified protein was separated by SDS–PAGE and stained with silver. The protein was in‐gel digested as described in Shevchenko et al. (1996) with the modification that the buffer contained 33% 18O‐labelled water. This leads to an isotopic labelling to 33% of all cleaved peptides at the C‐terminus. Peptides were extracted and analysed on an API III triple quadrupole tandem mass spectrometer (PE‐Sciex, Toronto, Canada) equipped with a nanoelectrospray ion source as described (Shevchenko et al., 1996; Neubauer and Mann, 1999). The peptides were sequenced de novo with mass spectrometry using a novel differential scanning technique. Briefly, two fragment spectra are acquired for each peptide, one from the 16O‐ and the 18O‐labelled peptide in one spectrum, the second only from the 18O isotope. This can be done without reducing the ion transmission through the instrument by increasing the resolution of the selecting quadrupole filter to isotopic resolution (M.Wilm, G.Neubauer, L.Taylor, A.Shevchenko and A.Bachi, in preparation). The sequence of the peptides was determined comparing the two fragment spectra by identifying C‐terminal fragment ions based on their different isotopic representation in both spectra.
Cloning of the C.fasciculata, T.brucei and L.tarentolae JBP genes
The following peptide sequence obtained from the purified C.fasciculata JBP was used successfully to design a degenerate oligonucleotide and clone a C.fasciculata cDNA: FTLHYPLR, corresponding to amino acids 743–750, was the basis for the complementary oligonucleotide 5′‐GCGAATTCGGGTAGTGVAKSGTGAA‐3′ containing an EcoRI site for cloning. This primer together with a primer specific for the mini‐exon were used to amplify first‐strand cDNA from C.fasciculata poly(A)+ RNA. A 1.8 kb PstI fragment from a cDNA with the correct predicted sequence was then used as a probe to make a genomic map of the C.fasciculata JBP locus and subsequently screen a size‐fractionated genomic library for a 7.5 kb HindIII–EcoRI fragment containing the JBP gene.
The 1.8 kb PstI C.fasciculata JBP probe was then used to identify homologous genes in T.brucei and L.tarentolae. For T.brucei, a size‐fractionated genomic library containing 1.3 kb HindIII fragments was screened under conditions of low stringency (filters were washed at 3× SSC, 0.1% SDS at 42°C). The resulting positive clone contained only part of the T.brucei JBP gene, so the 1.3 kb HindIII fragment was used to probe a size‐fractionated genomic library for a 7.5 kb SacI fragment containing the complete JBP gene. For L.tarentolae, the C.fasciculata JBP probe was used to screen a size‐fractionated genomic library (filters were washed at 0.1× SSC, 0.1% SDS at 55°C) for an 8 kb HindIII–ClaI fragment containing the complete JBP gene.
For all JBP gene clones, convenient restriction fragments were subcloned and sequenced.
Expression of recombinant JBPs in E.coli
Recombinant full‐length C.fasciculata and T.brucei JBPs were expressed in E.coli using the pET15b expression vector (Novagen). This system allows the production of recombinant protein containing an N‐terminal His6 tag to aid purification. Escherichia coli HMS 174(DE3)pLysS carrying the JBP expression plasmid were grown at 37°C to an A600 of 0.6, then isopropyl‐β‐d‐thiogalactopyranoside (IPTG) was added to 500 μM to induce protein expression and the cells allowed to grow for 7 h at 26°C. Cells were harvested by centrifugation, resuspended with lysis buffer (50 mM Tris–HCl pH 7.9, 500 mM NaCl, 5 μg/ml each of leupeptin and pepstatin) at 1/12.5 of the original culture volume, and frozen at −70°C. Following a 25°C thaw, the extract was passed several times through an 18 gauge needle. Cellular debris was removed by centrifugation at 10 000 g for 15 min at 4°C, and the resulting supernatant passed over 1 ml of TALON™ beads (Clontech) to purify the recombinant JBP, according to the manufacturer's instructions. Recombinant JBP was eluted with 50 mM imidazole. Approximately 5 ng of the resulting eluate were assayed for J‐specific DNA‐binding activity in the standard bandshift assay. Soluble C.fasciculata rJBP could be detected by Coomassie staining after SDS–PAGE, but soluble T.brucei rJBP could only be detected by Western blotting/ECL with antibodies against the His6 tag.
UV cross‐linking assay
J‐specific DNA binding was also assayed by UV cross‐linking. Binding reactions were carried out as described above, except that α‐casein was omitted and 0.2 pmol of probe were used. A 3.8 μg aliquot of T.brucei bloodstream form nuclear extract or 10 ng of T.brucei recombinant JBP was used per reaction. After binding, reactions were irradiated on ice in a Stratagene UV Stratalinker at 0.5 J/cm2. The samples were then boiled in loading buffer and proteins separated on an 8% polyacrylamide–SDS gel. Proteins cross‐linked to the labelled DNA oligo were detected by autoradiography.
We would like to thank Inês Chaves, Dennis Dooijes, Herlinde Gerrits, Rainer Mussmann and Ronald Plasterk for suggestions and critical reading of the manuscript, and Titia Sixma for help with protein structure predictions. M.C. was in part funded by an EC Marie Curie fellowship.
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