GPR1 encodes a putative G protein‐coupled receptor that associates with the Gpa2p Gα subunit and functions in a Ras‐independent pathway

Yong Xue, Montserrat Batlle, Jeanne P. Hirsch

Author Affiliations

  1. Yong Xue1,
  2. Montserrat Batlle1 and
  3. Jeanne P. Hirsch*,1
  1. 1 Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, NY, 10029, USA
  1. *Corresponding author. E-mail: hirsch{at}
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The yeast RAS1 and RAS2 genes appear to be involved in control of cell growth in response to nutrients. Here we show that this growth control also involves a signal mediated by the heterotrimeric G protein α subunit homolog encoded by GPA2. A GPA2 null allele conferred a severe growth defect on cells containing a null allele of RAS2, although either mutation alone had little effect on growth rate. A constitutive allele of GPA2 could stimulate growth of a strain lacking both RAS genes. Constitutive GPA2 conferred heat shock sensitivity on both wild‐type cells and cells lacking RAS function, but had no effect in a strain containing a null allele of SCH9, which encodes a kinase related to protein kinase A. The GPR1 gene was isolated and was found to encode a protein with the characteristics of a G protein‐coupled receptor. Double Δgpr1 Δras2 mutants displayed a severe growth defect that was suppressed by expression of the constitutive allele of GPA2, confirming that GPR1 acts upstream of GPA2. Gpr1p is expressed on the cell surface and requires sequences in the membrane‐proximal region of its third cytoplasmic loop for function, as expected for a G protein‐coupled receptor. GPR1 RNA was induced when cells were starved for nitrogen and amino acids. These results are consistent with a model in which the GPR1/GPA2 pathway activates the Sch9p kinase to generate a response that acts in parallel with that generated by the Ras/cAMP pathway, resulting in the integration of nutrient signals.

  • Received December 15, 1997.
  • Revision received February 4, 1998.
  • Accepted February 4, 1998.
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