Eukaryotic cells respond to DNA damage and S phase replication blocks by arresting cell‐cycle progression through the DNA structure checkpoint pathways. In Schizosaccharomyces pombe, the Chk1 kinase is essential for mitotic arrest and is phosphorylated after DNA damage. During S phase, the Cds1 kinase is activated in response to DNA damage and DNA replication blocks. The response of both Chk1 and Cds1 requires the six ‘checkpoint Rad’ proteins (Rad1, Rad3, Rad9, Rad17, Rad26 and Hus1). We demonstrate that DNA damage‐dependent phosphorylation of Chk1 is also cell‐cycle specific, occurring primarily in late S phase and G2, but not during M/G1 or early S phase. We have also isolated and characterized a temperature‐sensitive allele of rad3. Rad3 functions differently depending on which checkpoint pathway is activated. Following DNA damage, rad3 is required to initiate but not maintain the Chk1 response. When DNA replication is inhibited, rad3 is required for both initiation and maintenance of the Cds1 response. We have identified a strong genetic interaction between rad3 and cds1, and biochemical evidence shows a physical interaction is possible between Rad3 and Cds1, and between Rad3 and Chk1 in vitro. Together, our results highlight the cell‐cycle specificity of the DNA structure‐dependent checkpoint response and identify distinct roles for Rad3 in the different checkpoint responses.
Throughout the cell cycle, checkpoint pathways monitor the status of the DNA, and arrest cell‐cycle progression in response to DNA damage or inhibition of DNA replication (Hartwell and Weinert, 1989; Carr, 1995). The significance of the checkpoint response in maintaining genomic stability is highlighted by the evolutionary conservation of these pathways, as mammalian homologues of several yeast checkpoint genes, including those of rad1 (hRad1), rad3 (ATR/ATM), rad9 (hRad9), rad17 (hRad17), hus1 (hHus1) and chk1 (hChk1), have been identified. Phenotypes have only been identified for mammalian homologues of the rad3 gene (Savitsky et al., 1995; Bentley et al., 1996; Lieberman et al., 1996; Flaggs et al., 1997; Sanches et al., 1997; Cliby et al., 1998; Kostrub et al., 1998).
In fission yeast, Rad3 is one of six checkpoint Rad proteins required for both the DNA damage and DNA replication checkpoints (Bentley et al., 1996). However, these pathways can be differentiated by varying degrees of dependency on Chk1 kinase, which functions in a Rad3‐dependent manner in response to DNA damage (Al‐Khodairy et al., 1994; Ford et al., 1994; Walworth and Bernards, 1996). chk1 null mutants are sensitive to DNA damage but not hydroxyurea treatment. Consistent with this, chk1 null cells are unable to delay mitotic entry after DNA damage, but arrest normally in hydroxyurea. Chk1 is phosphorylated in response to DNA damage, although it is not known whether Chk1 phosphorylation corresponds to activation (Walworth and Bernards, 1996). Work in human cells suggests a model in which Chk1 targets the cell‐cycle machinery by inhibiting the Cdc25 phosphatase (Peng et al., 1997; Sanches et al., 1997).
The Cds1 kinase is activated in response to both DNA damage and DNA replication blocks only during S phase (Lindsay et al., 1998). Similar to Chk1 phosphorylation, this response is dependent on the presence of the checkpoint Rad proteins, including Rad3. Cds1 is required for the delay of S phase progression in response to DNA damage, and is also required for cells to tolerate replication blocks imposed by hydroxyurea. Although neither cds1 or chk1 mutants show a clear mitotic arrest defect during incubation with hydroxyurea, a cds1 chk1 double mutant is unable to delay mitosis during treatment, and the cells die with a classic ‘cut’ phenotype (Lindsay et al., 1998). Chk1, which is not normally phosphorylated during hydroxyurea treatment, becomes phosphorylated during hydroxyurea treatment in cds1 null cells (Lindsay et al., 1998). This suggests that Cds1 and Chk1 are not simply functionally redundant, as has been suggested in a recent report by Boddy et al. (1998), but that in the absence of the Cds1 protein kinase, hydroxyurea causes DNA damage which is then capable of activating the Chk1 pathway. It is clear that Cds1 plays an important role in regulating DNA synthesis.
The observation that Cds1 activation is confined to S phase implies that the checkpoint response is regulated in a cell‐cycle‐dependent manner. Here we present evidence that the Chk1 response is also cell‐cycle specific. Chk1 phosphorylation occurs during late S phase and G2 in response to both UV and gamma irradiation. During G1/early S phase and mitosis there is no significant change in the phosphorylation status of Chk1 in response to damage. As anticipated, the activation of the DNA damage checkpoint during S and G2 required the presence of rad3.
We have also isolated and analysed a temperature‐sensitive allele of rad3 (rad3ts). We find that rad3 function is different for the distinct checkpoint responses. After DNA damage, Rad3 is required to initiate mitotic arrest and phosphorylate Chk1. However, once initiated, these responses are maintained independently of Rad3. In response to DNA replication blocks, Rad3 is required for both initiation and maintenance of mitotic arrest and Cds1 activity. We have identified genetic interactions between rad3 and cds1. Together with biochemical data, which demonstrate that Rad3 can both bind to and phosphorylate the Cds1 and Chk1 kinases in vitro, these results suggest that the link between Rad3 and the downstream kinases is direct. We discuss the possibility that these data reflect the formation of different Rad3 protein complexes.
Cell‐cycle specificity of Chk1 phosphorylation
G2‐delay in response to DNA damage requires the function of the Chk1 protein. Following DNA damage of asynchronous cells, Chk1 is phosphorylated and this modification can be detected as a decrease in mobility by SDS–PAGE. In order to establish whether the DNA damage checkpoint is regulated by the cell cycle, we synchronized HA‐epitope tagged chk1 cells (chk1::HA; Walworth and Bernards, 1996) using cdc10 and cdc25. When incubated at the restrictive temperature, cdc10 and cdc25 arrest in the G1 and G2 phases of the cell cycle, respectively. When shifted back to the permissive temperature, cells will re‐enter the cell cycle synchronously. chk1::HA cells were also arrested in early S phase using a hydroxyurea block. These cells proceeded through S phase synchronously when hydroxyurea was removed from the medium. Irradiation with both gamma (250 Gy) and UV (100 J/m2) of arrested cells, and of cells at specific time points after release from arrest, showed that there was a significant increase in the amount of shifted (phosphorylated) Chk1 (compared with the total amount of non‐shifted protein) during late S phase and G2, but not during G1/early S phase or mitosis (Figure 1A and B). These results were confirmed by irradiating (100 Gy) cdc+ chk1::HA cells that had been synchronized by elutriation (Figure 1C). If Chk1 functions after irradiation in G1/early S phase, these results indicate that this is distinct from its function during S phase and G2. To rule out the possibility that the DNA content of the cells is responsible for these observations, we analysed chk1::HA diploid cells. These cells behaved in a similar manner to the chk1::HA haploid cells after gamma irradiation (data not shown).
Isolation and characterization of rad3ts mutant allele
Schizosaccharomyces pombe rad3 is required for all DNA structure‐dependent checkpoints (Bentley et al., 1996). To understand the role of rad3 we isolated a temperature‐sensitive allele of rad3 (rad3ts) by transforming a rad3 null strain (rad3.d) with a randomly mutagenized genomic rad3 plasmid. A total of 60 000 colonies were screened for sensitivity to hydroxyurea at 35°C but not at 27°C. One plasmid was chosen for further analysis. Gene replacement of the rad3 deletion allele with the rad3ts allele confirmed that rad3ts gene conferred a clearly defined temperature‐sensitive phenotype. Analysis of survival following either UV or hydroxyurea treatment, plus analysis of the checkpoint response to gamma‐irradiation and hydroxyurea treatment, revealed that the rad3ts strain behaved like wild‐type cells at the permissive temperature (27°C) but had a phenotype equivalent to the rad3 null mutant at the restrictive temperature (35°C) (Figure 2A–C). Sequence analysis of the rad3ts plasmid revealed the presence of seven mutations, one of which was located at a conserved residue within the C‐terminal kinase domain, in close proximity to the ATP‐binding pocket (A2217V) (Figure 2D). We have confirmed that the A2217V mutation resulted in a similar phenotype to rad3ts by repeating the genomic integration using a rad3 sequence containing only this point mutation (data not shown).
Analysis of rad3ts following DNA damage
Although it is known that rad3 is required for the establishment of the DNA damage checkpoint response and for Chk1 phosphorylation, the temporal requirements for Rad3 are not known. When rad3ts cells were incubated at the restrictive temperature for 10 min prior to gamma or UV irradiation, cells behaved similarly to the rad3.d strain, with a significant decrease in cell survival and a complete absence of the DNA damage checkpoint (Figure 3A–C). In contrast, when rad3ts cells were shifted to the restrictive temperature, 5 or 10 min after irradiation with gamma or UV, the cells survived equally as well as wild‐type cells and maintained a virtually intact checkpoint response (Figure 3A–C). In addition, both cell survival and the damage checkpoint response (after UV and gamma irradiation) were similar in cells shifted to the restrictive temperature at different time intervals following irradiation (Figure 3A–C). These results suggest that rad3 is required to initiate the DNA damage checkpoint response, but that once activated, the response is maintained independently of rad3.
Phosphorylation of the Chk1 protein kinase
In wild‐type cells there is a temporal correlation between mitotic delay and Chk1 phosphorylation, as determined by decreased mobility on SDS–PAGE (Walworth and Bernards, 1996). To investigate whether Chk1 phosphorylation correlates with our visual analysis of the checkpoint, we created a rad3ts strain containing the genomic chk1::HA locus described by Walworth and Bernards (1996). At the permissive temperature (27°C) for the rad3ts allele, a decrease in the mobility of Chk1 was observed in response to gamma and UV irradiation, indicating that Chk1 was phosphorylated as a result of checkpoint pathway activation (Figure 4; data not shown). At the restrictive temperature (35°C), these modifications were not observed (Figure 4; data not shown). If the rad3ts chk1::HA strain was shifted to the restrictive temperature 10 min prior to irradiation, the Chk1 protein was not modified (Figure 4; data not shown). In contrast, if the rad3ts chk1::HA cells were shifted to the restrictive temperature 10 min after irradiation, Chk1 was modified to similar levels as seen in wild‐type cells (Figure 4; data not shown). These results are consistent with the physiological observations, and show that rad3 is primarily required to initiate the DNA damage checkpoint response and that once the checkpoint has been activated, this response is largely maintained independently of rad3.
Analysis of rad3ts in response to replication blocks
rad3 is required for the response to DNA replication blocks and for the activation of the Cds1 protein kinase during S phase, in response to both DNA replication blocks and DNA damage (Lindsay et al., 1998). Analysis of the rad3ts strain indicated that at 27°C, survival of hydroxyurea exposure and activation of the replication checkpoint was similar to wild‐type cells, while at 35°C it was similar to rad3.d cells (Figure 2A). Interestingly, the checkpoint response established by the rad3ts strain at the permissive temperature could not be maintained at the restrictive temperature and cells entered a catastrophic mitosis in a synchronous manner following a temperature shift to 35°C (Figures 2C and 5). A 60 min delay was observed between the time of temperature shift and subsequent entry into mitosis. This was observed even when cells had been incubated at the permissive temperature with hydroxyurea for a prolonged period.
Cds1 protein kinase activity
Cds1 activation is dependent on the ‘checkpoint rad’ genes. Cds1 is essential for the delay to S phase progression in response to DNA damage and is also essential for tolerance to replication blocks imposed by incubation with hydroxyurea (Murakami and Okayama, 1995; Lindsay et al., 1998). To determine whether the loss of cell‐cycle arrest observed in rad3ts cultures shifted to the restrictive temperature was related to the activity of the Cds1 protein kinase, we assayed for Cds1 protein kinase activity on the non‐physiological substrate, myelin basic protein (MBP). After shifting to the restrictive temperature, Cds1 protein kinase activity was reasonably high between 30 and 60 min, but beyond this time a decay was observed and Cds1 kinase activity was almost non‐detectable (Figure 5B). Thus, there is a strong correlation between the decrease in Cds1 kinase activity and subsequent entry into a catastrophic mitosis.
Taken together these results suggest that rad3 is required to both initiate and maintain the checkpoint response to DNA replication blocks. The temporal correlation between the decrease in Cds1 protein kinase activity and the time which cells enter a catastrophic mitosis suggests a functional dependency.
Multi‐copy suppressor screen for rad3ts hydroxyurea survival
The creation of the rad3ts allele gave us a tool to identify genes which genetically interacted with rad3. Screens were initiated to identify multi‐copy suppressors of the sensitivity of the rad3ts mutant to hydroxyurea at the semi‐permissive temperature (32°C). Of the 150 multi‐copy suppressors isolated, 40 were characterized as strong suppressors of the rad3ts hydroxyurea sensitivity. Sequence and Southern‐blot analysis revealed that 36 of these were plasmids containing the rnr3 gene, which encodes the catalytic subunit of ribonucleotide reductase and functions to maintain nucleotide pools. Given that hydroxyurea reduces nucleotide pools by inhibiting ribonucleotide reductase, it is perhaps not surprising that multiple clones of rnr3 were isolated. This presumably represents a classic bypass of the requirement for rad3 function, since cells do not elongate under these circumstances (data not shown).
Analysis of the remaining four strong suppressors identified the cds1 gene. The genetic interaction between rad3 and cds1 was found to be specific to the rad3ts allele, and specific to the semi‐permissive conditions at the expression levels used (multi‐copy genomic fragment under its own promoter). cds1 was neither able to suppress the hydroxyurea sensitivity of the rad3.d mutant (Figure 6A) or the hydroxyurea sensitivity of the rad3ts mutant at the restrictive temperature of 35°C (data not shown). These results represent a genetic interaction between rad3 and cds1: cds1 suppression requires the Rad3 protein in a partially active form, suggesting a close link exists between these proteins.
Chk1 is a multi‐copy suppressor of rad3ts UV sensitivity
Suppression of the sensitivity of the rad3ts mutant to replication blocks by the downstream kinase Cds1, suggested that the Chk1 kinase might suppress the sensitivity of rad3ts to DNA damage in a manner specific to the rad3ts allele. Analysis of survival to DNA damage of rad3ts and rad3 null cells over‐expressing chk1 demonstrated that chk1 was able to suppress the UV sensitivity of both the rad3ts and rad3.d mutants at the semi‐permissive temperature for the rad3ts allele (Figure 6B). chk1 over‐expression is known to delay the cell cycle independently of rad3 (Ford et al., 1994) and even a moderate cell‐cycle delay has been previously shown to partially rescue survival of checkpoint rad mutants (Al‐Khodairy and Carr, 1992). Thus, a genetic interaction, if it exists, might be masked by non‐specific effects.
Rad3 interacts with Cds1 and Chk1
To explore the interactions identified between rad3 and cds1, and possibly between rad3 and chk1, we asked whether the corresponding proteins interact biochemically. Co‐immunoprecipitation experiments were performed using a genomic myc‐tagged allele of rad3 (described elsewhere) but neither Cds1 nor Chk1 proteins at wild‐type levels or when over‐expressed using a plasmid‐borne attenuated nmt1 promoter could be detected (IP of myc‐tagged Rad3 requires cell lysis in mild detergent). However, when glutathione S‐transferase (GST)‐tagged Chk1 or Cds1 were co‐overexpressed with myc‐tagged Rad3, Rad3 was identified in both Chk1 and Cds1 precipitates (Figure 6C). When both HA‐tagged Rad3 and myc‐tagged Chk1 were overexpressed in wild‐type cells (we estimate the level of over‐expression to be ∼10‐ to 15‐fold), Chk1 was identified in Rad3 immunoprecipitates (Figure 6D). Under equivalent conditions Cds1 was not identified in Rad3 immunoprecipitates. These data suggest that Rad3 may interact with Cds1 and Chk1 in a transient manner which can only be detected by immunoprecipitation following over‐expression. To address whether Cds1 and Rad3 are found in a common complex in the soluble material (equivalent to that used for IP), we examined the size distribution of these two proteins using Superdex 200 column chromatography. In soluble extracts, Rad3 and Cds1 did not elute in common fractions (Figure 6F and G). We do not consider that the interactions detected when the proteins are over‐expressed are artefactual, since interactions between Rad3 and several other checkpoint proteins such as Rad1 and Rad17 were not identified using equivalent conditions (data not shown).
Rad3 phosphorylates Cds1 and Chk1 in vitro
Genetic suppression of the rad3.ts mutant strain by cds1 suggests that the kinase encoded by this gene is able to amplify the presumably weakened checkpoint signal transmitted by the defective form of the Rad3 protein, and therefore maintain the checkpoint response. In addition to the genetic interaction, Rad3‐dependent activation (Cds1) and modification (Cds1 and Chk1) suggest that these kinases may be substrates for Rad3. The potential for interactions identified between Rad3–Cds1 and Rad3–Chk1 would be consistent with a kinase–substrate relationship between Rad3 and these downstream kinases. To investigate this we tested kinase dead (KD) Cds1 and Chk1 as in vitro substrates for Rad3, immunoprecipitated from cells overexpressing rad3. Although the Rad3 protein has relatively weak in vitro kinase activity and does not phosphorylate a number of non‐physiological substrates (Bentley et al., 1996), immunoprecipitated Rad3 was shown to phosphorylate Cds1‐KD and Chk1‐KD substrates (Figure 6E). Control experiments using immunoprecipitated Rad3.KD indicate that Cds1 and Chk1 phosphorylation was dependent on the integrity of the Rad3 kinase. In these assays Chk1 is not converted to a lower mobility form. This may be because a kinase dead form of Chk1 was used and it has been reported that Chk1 kinase activity is required to see the mobility change (Walworth and Bernards, 1996). These results show that the Rad3 kinase is capable of phosphorylating both Cds1 and Chk1 protein kinases in vitro, although it does not establish that they are direct targets in vivo. The inability to detect Rad3 kinase activity using a variety of non‐physiological substrates, such as MBP, lends support to a biological significance for the phosphorylation of Cds1 and Chk1 by Rad3. Further analysis to establish whether or not this phosphorylation resulted in the activation of Cds1 and Chk1 by Rad3 proved inconclusive. This might be expected, given the complexity of the checkpoint response and that additional factors may be required for the activation of Rad3 substrates, which were not present in the in vitro conditions used.
Two main points emerge from our analysis of Rad3 and Chk1: first, the response of Chk1 to DNA damage is specific to the later stages of the cell cycle (post DNA replication). Secondly, the function of Rad3 in activating the Cds1 (S phase specific) and Chk1 (late S/G2 phase specific) responses is distinct. For the Chk1 response, Rad3 is required to initiate but not to maintain mitotic delay. For the Cds1 response, Rad3 is required for both initiation and maintenance. We also present genetic and biochemical data that are consistent with a direct interaction between Rad3 and these two downstream kinases.
Cell‐cycle specificity of checkpoint responses
The chk1 null mutant is sensitive to DNA damaging agents and is unable to delay mitotic entry in response to DNA damage (Walworth et al., 1993; Al‐Khodairy et al., 1994). Chk1 is phosphorylated after DNA damage, and there is a temporal correlation between Chk1 phosphorylation and the activation of the checkpoint response. Analysis in human cells suggests Chk1 prevents entry into mitosis following DNA damage by targeting the cell‐cycle machinery through inhibition of the Cdc25 phosphatase (Sanches et al., 1997). This is consistent with data from S.pombe (Furnari et al., 1997), although it has also been reported that Wee1 is a downstream target of Chk1 (O'Connell et al., 1997). The precise nature of the interactions between Chk1 and the cell‐cycle machinery thus awaits identification of the in vivo targets.
Our data provide evidence that activation of the DNA damage checkpoint is cell‐cycle specific. Chk1 is phosphorylated in response to damage during late S phase and G2. During G1/early S phase and mitosis there was no significant modification of Chk1 in comparison with the total amount of non‐shifted Chk1 protein. chk1::HA diploid cultures had a similar Chk1 phosphorylation profile in response to both UV and gamma irradiation, indicating that the cell‐cycle specificity identified for the DNA damage checkpoint is not caused simply by the DNA content of the cell. It is known that the Chk1 protein kinase is required to prevent catastrophic mitosis in cdc10 arrested cells (G1 phase cells), which suggested a role for the Chk1 protein kinase in G1 (Carr et al., 1995). From our data it is clear that if the Chk1 protein kinase performs different roles (or performs the same role in a different manner) during specific stages of the cell cycle, the onset of S phase and the beginning of DNA replication are required for the switch from the G1‐form of response to the G2‐form of response, which is seen following DNA damage.
Interestingly, a very weak modification of Chk1 protein in non‐irradiated cells can be seen at t30 and t60 (S phase), but not at t0, t90 and t120 after the release of the hydroxyurea block. This suggests that problems are caused by incubation of cells in hydroxyurea, but that these only cause modification of the Chk1 protein kinase once cells progress through S phase.
Cell‐cycle‐ and checkpoint‐specific roles of Rad3
Characterization of the rad3ts mutant showed a null phenotype at the restrictive temperature (35°C) and a wild‐type phenotype at the permissive temperature (27°C). We have used this allele to investigate the temporal requirement for rad3 in the checkpoint responses. rad3 is primarily required to initiate the DNA damage checkpoint. Once activated, this checkpoint is maintained largely independently of rad3. When the rad3ts strain was incubated at the restrictive temperature 10 min prior to UV or gamma irradiation, the DNA damage checkpoint was defective (determined by cell survival, cell‐cycle arrest and Chk1 phosphorylation). When the rad3ts strain was shifted to the restrictive temperature, 5 or 10 min after irradiation, the cells behaved in a similar manner to wild‐type cells.
The difference in phenotypes between cells shifted to the restrictive temperature 10 min before or 10 min after UV and gamma irradiation, and the fact that cells shifted to the restrictive temperature at different time points post‐irradiation show a similar survival and duration of cell‐cycle arrest, indicates that the Rad3ts protein is not simply being inactivated slowly. The similar response of a temperature‐sensitive allele of another DNA damage checkpoint gene, rad4/cut5 (unpublished data; McFarlane et al., 1997), suggests that these results reflect the overall organization of the DNA damage checkpoint and are not caused by an unusual feature of the rad3ts allele itself.
In contrast to the DNA damage checkpoint role, Rad3 is required to both activate and maintain the response to replication blocks. When rad3ts cells were incubated with hydroxyurea at the permissive temperature (27°C), the checkpoint response activated could not be maintained when cells were shifted to the restrictive temperature (35°C). The synchronous entry into mitosis observed at the restrictive temperature suggests that the Rad3ts protein is not slowly inactivated. Interestingly, a 60 min delay was observed between the shift to the restrictive temperature and entry into mitosis. The kinetics of this delay correlated with a decrease of Cds1 protein kinase activity, which was observed between 30 and 60 min.
The cds1 gene was isolated four times from a screen for multi‐copy suppressors of the hydroxyurea sensitivity of rad3ts cells at the semi‐permissive temperature (32°C). This suppression was specific to the rad3ts allele and did not occur at the restrictive temperature, suggesting a close association between rad3 and cds1. Attempts were made to detect a physical interaction between these two proteins at physiological levels. These demonstrated that Rad3 and Cds1 are not associated in soluble extracts. However, under conditions in which both Cds1 and Rad3 were moderately overexpressed (we estimate ∼10‐ to 15‐fold over wild‐type levels), Rad3 was reproducibly found in Cds1 precipitates. Furthermore, Rad3 could phosphorylate Cds1 in vitro. Together, these data indicate that Rad3 is capable of forming a kinase–substrate interaction with Cds1, and that the connection between these kinases is direct and does not require additional factors. It should also be remembered that co‐precipitation and co‐size fractionation experiments only address the state of interactions in soluble material. It remains possible that a weak but temporally long‐lasting interaction may occur in conjunction with DNA and DNA associated proteins that is not accessible to such techniques.
Using similar genetic and biochemical tools, we also asked whether Chk1 and Rad3 could associate. When overexpressed, the chk1 gene is able to partially suppress the UV sensitivity of the rad3ts mutant at the restrictive temperature. Yet, since chk1 is also able to suppress the UV sensitivity of the rad3 null mutant to equivalent levels as the rad3ts allele, the specificity of this suppression is unclear, especially when it is considered that chk1 over‐expression delays cell‐cycle progression independently of rad3 (Ford et al., 1994). The over‐expression of chk1 and rad3 in wild‐type cells, and subsequent precipitation of the Chk1 protein with Rad3 and vice versa, supported an interaction between Rad3 and Chk1, although this interaction did not occur at physiological levels of protein. Rad3 was able to phosphorylate Chk1 in vitro. Combined with the genetic data available, this suggests a direct link between Rad3 and Chk1.
A model for the cell‐cycle specificity of checkpoint responses
Cds1 activation is S phase specific and Chk1 phosphorylation is late S/G2‐phase specific. The role of Rad3 in these two events is also distinct. Since the Rad3 protein is required for all DNA structure dependent checkpoint responses, a model that could account for the cell‐cycle specificity would be the formation and/or utilization of different populations of Rad3 protein complexes during the cell cycle. At different stages of the cycle, Rad3 could be associated with different proteins (e.g. Cds1 and Chk1) and these might modulate its function.
The different temporal requirements for Rad3 in the Chk1 and Cds1 responses probably reflects the different functions of the two checkpoints. DNA damage appears to be rapidly recognized by the checkpoint machinery and signalled to Chk1, which then maintains the checkpoint for a period of time consistent with the phosphorylation status of Chk1. Thus, Chk1 dephosphorylation may be the key event in determining the duration of the delay. Such a model would define a period for repair which would be based on the initial intensity of the damage. In this way, the damage checkpoint would be independent of the DNA or DNA–protein structures which are generated by subsequent repair. Unlike the damage checkpoint, which is a response to sudden events, the DNA replication checkpoint monitors an ongoing process. The associated DNA and DNA protein structures would therefore be persistent. The replication checkpoint would be expected to respond to release from the replication block quite rapidly. Thus, the constant requirement for Rad3 is consistent with the continuous monitoring of a stable structure until the situation is resolved.
In humans, different complexes are likely to be required during different stages of the cell cycle. The two Rad3 related proteins ATM and ATR appear to share responsibility for the DNA structure checkpoints (Savitsky et al., 1995; Cliby et al., 1998). ATR and ATM complexes might be expected to monitor a number of distinct DNA structures, and the occurrence of these protein complexes might be associated with passage through G1, S and G2 phases of the cell cycle. Our analysis of the different modes of action for Rad3 in fission yeast and the differential phosphorylation of Chk1 during the cell cycle may therefore help to provide insight into the cellular mechanisms that maintain genomic integrity in humans.
Materials and methods
The strains used in this study are listed in Table I.
Genetic and cell biology techniques
Double mutants were created by standard genetic techniques (Gutz et al., 1974). Cell survival analysis and checkpoint measurements following DNA damage and replication blocks were performed using methods described previously (Al‐Khodairy et al., 1994). Cells were synchronized in G2 using centrifugal force [lactose gradients or elutriation (Edwards and Carr, 1997)], or a 4 h temperature shift from 27 to 35°C of the temperature‐sensitive mutant strain cdc25.22. Cells were synchronized in G1/S phase by either a 4 h temperature shift from 27 to 35°C of the temperature‐sensitive mutant strain cdc10.129, or by hydroxyurea‐induced DNA replication block (20 mM) and release by washing cells in fresh medium.
We noticed that, possibly due to the centrifugal stress cells suffer during the elutriation procedure, there is a decrease of the total amount of Chk1 protein in the first two time‐points after harvesting (this effect appears to be reproducible), and that a high degree of synchrony is required to detect a clear absence of Chk1 protein modification after DNA damage during mitosis and G1/early S phase because these periods are relatively short.
Isolation of a thermosensitive allele of rad3
The complete rad3 gene, cloned as a SacI–PstI fragment in the leucine selectable expression vector pAL, was mutagenized with hydroxylamine (Humphreys et al., 1976). Mutagenized plasmids were transformed into rad3.d and the resulting colonies selected on medium lacking leucine. To identify thermosensitive alleles of the rad3 gene, 60 000 colonies were screened by replica plating onto plates containing either 7.5 or 10 mM hydroxyurea at both 27°C and 35°C, respectively (Martinho and Carr, 1998). Twenty colonies which were able to grow at 27°C, which possessed an elongated cellular phenotype and which died at 35°C with a ‘cut’ phenotype were selected. Further characterization yielded a single colony with a well‐defined phenotype under these conditions. To generate a rad3ts strain, the plasmid from this colony was isolated and the SacI–PstI fragment encoding the mutagenized rad3 gene transformed into the rad3.d using FOA selection (Grimm et al., 1988). Genomic integration and replacement of the ura4+ marker in the rad3.d strain by the mutated rad3 clone was confirmed by Southern blotting. Sequence analysis was used to identify the mutations present in the rad3 clone. The causative mutation of the temperature‐sensitive phenotype (A2217V) was confirmed by genomic integration.
Isolation of multi‐copy suppressors of rad3ts
Unless stated otherwise, the semi‐permissive temperature used was 32°C and the restrictive temperature 35°C. The rad3ts strain was transformed with the pURSP1 and pURSP2 S.pombe genomic libraries (Barbet et al., 1992), and 50 000 colonies were screened by replica plating onto plates containing 4, 5 and 7.5 mM hydroxyurea at both the semi‐permissive and restrictive temperature, respectively (Martinho and Carr, 1998). Approximately 150 colonies, which were able to grow at the semi‐permissive temperature but not at the restrictive temperature in the presence of hydroxyurea, were isolated and characterized further. To identify the suppressers of the rad3ts hydroxyurea sensitivity, the library plasmids from these clones were recovered and analysed by restriction, Southern blot and sequence analyses. Analysis revealed multiple isolates of the rnr3 gene and the isolation of four cds1 clones. To characterize further cds1 suppression, cds1 under the control of its own promoter or the inducible nmt1 promoter, was transformed into both the rad3ts and rad3.d strains. The resulting transformants were serially diluted (107–104 cells/ml) and 2 μl were spotted onto plates with or without hydroxyurea (7.5 mM); plates were then incubated at the semi‐permissive temperature. To analyse the suppression of rad3 by chk1, the chk1 gene under the control of the inducible nmt1 promoter (Rep41) was transformed into both the rad3ts and rad3.d strains, which were assayed for survival with increasing doses of UV radiation (Edwards and Carr, 1997).
Cds1 kinase assays
Protein extraction and Cds1 kinase assays were performed as previously described (Lindsay et al., 1998). One milligram of total protein was immunoprecipitated using an affinity purified Cds1 kinase polyclonal antibody and protein A beads. The beads were washed three times with kinase buffer prior to incubation with 5 μl myelin basic protein (1 mg/ml of stock), 1 μl 2 mM ATP and 5 μCi [γ‐32P]ATP (ICN) at 30°C for 15 min. The reaction was stopped by the addition of 20 μl of 2× sodium dodecyl sulfate (SDS) sample buffer, and Cds1 kinase activity was analysed by SDS–PAGE.
Immunoprecipitation with Rad3
Wild‐type cells were transformed with either Rep1GSTcds1 or Rep1GSTchk1 and myc‐tagged rad3, or with Rep41 and/or Rep42 plasmids (enabled nmt1 inducible expression) containing HA‐tagged rad3 or myc‐tagged chk1, respectively (Griffiths et al., 1995; Bentley et al., 1996). The tagged proteins were expressed by growing in media lacking thiamine for 18 h (Maundrell, 1990). Yeast cells were lysed and 1 mg total protein was immunoprecipitated using appropriate antibodies, as described previously (Bentley et al., 1996). The immunoprecipitates were run on 8% polyacrylamide gels. Anti c‐Myc (PharMingen) and anti‐HA (Babco) antibodies, at dilutions of 1:1000, were used to probe the subsequent Western blots. Chemiluminescent detection of horse radish peroxidase (HRP)‐conjugated secondary antibodies was carried out using ECL (Amersham).
Extracts were prepared from a strain containing the rad3 gene tagged at the genomic locus with the myc epitope as follows: mid‐log phase cells were harvested, washed in ice‐cold water, washed again in ice‐cold buffer B (50 mM NaH2PO4/Na2HPO4 pH 7.0, 150 mM NaCl, 0.1% NP‐40, 10% glycerol, 0.5 mM DTT, 5 mM EGTA, 60 mM β‐glycerophosphate, 0.1 mM NaF, 1 mM Na‐orthovanadate) and resuspended in 1 ml buffer B plus protease inhibitors (BCL complete protease inhibitor cocktail). Cells were frozen in liquid nitrogen and ground to a fine powder using a mortar and pestle. Thawed powder was cleared by centrifugation (14 K in a microfuge 2× 5 min) and 200 μl of supernatant loaded onto a Superdex 200 column (Pharmacia HR10/30). Fractions (0.5 ml) were collected and aliquots analysed by SDS–PAGE and Western blotting with anti‐Myc monoclonal or anti‐Cds1 serum (Lindsay et al., 1998).
Kinase assays with Rad3
rad3.d was transformed with the nmt1 inducible expression vector containing either Rep41HA‐rad3 or Rep41HA‐rad3.KD, and the corresponding tagged proteins were induced in medium lacking thiamine. Protein extracts were prepared as described previously and 1 mg total protein immunoprecipitated with 1 ug anti‐HA antibody (Babco).
KD mutations were introduced into the cds1 and chk1 genes by site‐directed mutagenesis. A D318E mutation was introduced into the cds1 gene using the oligonucleotide GCCAAGCCAAATTCAGATATTTTAAGATGG, and a D155E mutation introduced into the chk1 gene using the oligonucleotide AGATGCAAAGCCGAATTCTGAAATCTTTAAG. Both mutations disrupt the conserved DFG motif in the kinase domain. The mutations were confirmed by sequence analysis. Cds1.KD and Chk1.KD protein kinase substrates were prepared by transforming cds1.d and chk1.d mutant strains with inducible plasmids containing GST‐tagged cds1.KD and chk1.KD genes, Rep1GSTcds1.KD and Rep1GSTchk1.KD, respectively. GST‐fusion proteins were expressed by growing in medium lacking thiamine for 18 h. The cells were disrupted in GST buffer (50 mM Tris pH 7.5, 80 mM β‐glycerophosphate, 250 mM NaCl, 15 mM nitrophenylphosphate, 50 mM NaF, 5 mM EDTA, 1 mM DTT, 0.1% NP‐40 and supplemented with protease inhibitors, AEBSF, leupeptin, aprotinin, pepstatin, bestatin, and E‐64, all at 10 μg/ml final concentration) with glass beads (BDH) using a mikrodismembranator (Braun) for 3× 1 min at 2000 r.p.m. Protein extracts were cleared in a microfuge at 14 000 r.p.m. for 5 min at 4°C. 1 mg total protein was mixed with glutathione–agarose beads (Sigma) for 1 h at 4°C, the beads were washed (×4) with GST buffer at 4°C, and the bound GST fusion proteins eluted in batch with 50 mM Tris (pH 8.0) containing 10 mM glutathione. The eluates were analysed by SDS–PAGE and then frozen as aliquots in liquid nitrogen.
Rad3 or Rad3.KD proteins were immunoprecipitated as described above. The beads were washed three times with lysis buffer and once with kinase buffer (10 mM HEPES pH 7.5, 75 mM KCl, 5 mM MgCl2, 0.5 mM EDTA, 1 mM DTT). Twenty microlitres of bead slurry was incubated with 10 μl 2× kinase buffer, 5 μl substrate and 10 μCi of [γ‐32P]ATP (ICN) at 30°C for 15 min. The reaction was stopped by the addition of 20 μl 2× SDS sample buffer and samples were analysed by SDS–PAGE.
We would like to thank Thomas Caspari for interesting and helpful discussions, and Thomas Caspari and Alan Lehman for critical reading of this manuscript. We would also like to thank Per Christenson for assistance with FACS analysis and Rhian Edwards for assistance with mutagenesis. R.G.M. is sponsored by the Portuguese Gulbenkian PhD program in Biology and Medicine.
- Copyright © 1998 European Molecular Biology Organization