The molecular mechanisms underlying gravity perception and signal transduction which control asymmetric plant growth responses are as yet unknown, but are likely to depend on the directional flux of the plant hormone auxin. We have isolated an Arabidopsis mutant of the AtPIN2 gene using transposon mutagenesis. Roots of the Atpin2::En701 null‐mutant were agravitropic and showed altered auxin sensitivity, a phenotype characteristic of the agravitropic wav6‐52 mutant. The AtPIN2 gene was mapped to chromosome 5 (115.3 cM) corresponding to the WAV6 locus and subsequent genetic analysis indicated that wav6‐52 and Atpin2::En701 were allelic. The AtPIN2 gene consists of nine exons defining an open reading frame of 1944 bp which encodes a 69 kDa protein with 10 putative transmembrane domains interrupted by a central hydrophilic loop. The topology of AtPIN2p was found to be similar to members of the major facilitator superfamily of transport proteins. We have shown that the AtPIN2 gene was expressed in root tips. The AtPIN2 protein was localized in membranes of root cortical and epidermal cells in the meristematic and elongation zones revealing a polar localization. These results suggest that AtPIN2 plays an important role in control of gravitropism regulating the redistribution of auxin from the stele towards the elongation zone of roots.
All plants exhibit gravitropic growth, but how they sense gravity and transduce this physical stimulus into physiological responses is a central question in plant biology (Firn and Digby, 1980; Pickard, 1985; Moore and Evans, 1986; Moore, 1991; Nedukha, 1997; Sievers et al., 1997). Plants respond to gravity by directional growth orientating roots vertically downwards (positive gravitropism) and shoots upwards (negative gravitropism). It is a common view that roots perceive gravity in the columella cells of the root cap through the sedimentation of dense starch grains (amyloplasts or statoliths) onto endoplasmic reticulum complexes, the cytoskeleton or the plasma membrane (Baluska and Hasenstein, 1997). This mechanism is believed to initiate a signal transduction cascade which subsequently transfers the gravity signal to cells of the root elongaton zone inducing differential cell elongation. As a result of asymmetric elongation, root curvature is induced (Young et al., 1990; Evans, 1991).
Since Cholodny (1927) and Went (1933) found an involvement of directional auxin transport in gravitropic responses, numerous studies have confirmed that this plant hormone is indeed essential for the mediation of tropic growth responses (Kaufman et al., 1995). Polar auxin transport occurs from auxin‐synthesizing tissues (e.g. young leaves or the shoot apex) through the vascular tissues of stems towards the root tips (Lomax et al., 1995). In roots, auxin is transported within stelar tissues in an acropetal direction towards the root tip. It has been proposed that around the meristematic zone auxin is loaded from the central stele into the cortical and epidermal cell layers where it is transported backwards in basipetal orientation to the elongation zones (Tsurumi and Ohwaki, 1978; Estelle, 1996).
Polar transport of auxin from cell to cell is thought to be primarily mediated by specific efflux carrier proteins polarly located at one end of transport‐competent cells. This polar localization of carrier proteins is hypothesized to be responsible for the polarity of auxin transport (Lomax et al., 1995). The elucidation of the localization of these auxin carriers in plant cells is expected to provide evidence supporting the Cholodny–Went hypothesis. To understand the mechanisms regulating auxin redistribution and tropic responses, physiological studies have been performed over several decades (reviewed in Kaufman et al., 1995), but despite extensive information, we still lack a detailed understanding of the molecular aspects underlying these processes. To dissect genetically the components of this pathway, mutants affected in various steps of gravity perception and transduction including the regulation of polar auxin transport were isolated (Maher and Martindale, 1980; Mirza et al., 1984; Kelly and Bradford, 1986; Eason et al., 1987; Caspar and Pickard, 1989; Bell and Maher, 1990; Okada and Shimura, 1990; Simmons et al., 1995; Yamauchi et al., 1997). The gene for one of these agravitropic mutants, aux1, has been cloned and shown to encode a permease‐like protein that has been proposed to encode a component of the auxin uptake carrier (Bennett et al., 1996).
Here we describe the molecular characterization of another agravitropic mutant, Atpin2::En701, isolated from an En‐1 transposon mutagenized Arabidopsis population. Atpin2::En701 displays severe defects in root growth and gravitropism, and is allelic to wav6‐52, a mutant which was previously shown to be allelic to agr1 (Bell and Maher, 1990; Okada and Shimura, 1990). The AtPIN2 gene encodes a protein showing structural similarity to the major facilitator‐like transporter family. The molecular properties and cellular expression profile of AtPIN2p suggested a function in polar auxin transport mediating the gravitropic growth in Arabidopsis roots.
Isolation and structure of the AtPIN2 gene
The AtPIN2 gene and the corresponding cDNA were isolated using a probe derived from the AtPIN1 gene which is involved in the control of polar auxin transport in Arabidopsis plants (L.Gälweiler, C.Guan, A.Müller, E.Wisman, K.Mendgen, A.Yephremov and K.Palme, submitted). A genomic clone was obtained spanning a region of ∼4 kb. Screening of several cDNA libraries resulted only in truncated AtPIN2 cDNAs. Therefore, we reconstructed a full‐length cDNA clone by PCR amplification of 269 bp from the genomic clone and ligation of this PCR fragment to the 5′ end of an incomplete cDNA. Sequence comparison of the genomic clone with the cDNA showed that the coding sequence of the AtPIN2 gene was interrupted by eight introns (Figure 1). Several in‐frame stop codons upstream of the coding region of 1944 bp and polyadenylation signals located at cDNA positions 1967, 2088 and 2135 indicated that the cDNA contained the full coding region. In addition, the sequence around the initiation codon confirmed Kozak's rules (Kozak, 1991). To determine the map position of the AtPIN2 gene, we hybridized the CIC YAC library with an AtPIN2 probe and identified a YAC contig consisting of CIC9E2, CIC10B4 and CIC9C9 (Creusot et al., 1995) located in the region of 115.34 cM (m558A) and 115.26 cM (mi70) on the lower arm of Arabidopsis chromosome 5 (Figure 1).
AtPIN2p is a protein with structural similarity to transmembrane transporters
The AtPIN2 gene encodes a protein of 647 amino acids with a predicted molecular mass of 69 kDa (Figure 2A). The predicted AtPIN2 protein sequence consisted of >35% hydrophobic amino acids, yielding a calculated isoelectric point of 9.37 and a net charge of +12.36 at pH 7.2. The protein showed high similarity to the AtPIN1 protein (64% identity; L.Gälweiler, C.Guan, A.Müller, E.Wisman, K.Mendgen, A.Yephremov and K.Palme, submitted), and to other members of the PIN gene family (unpublished data). Hydropathy analysis revealed three characteristic regions: a hydrophobic region with five to six transmembrane segments (residues 1–163), a predominantly hydrophilic core extending from residue 164 to 482 which was followed by another hydrophobic region with about four to five transmembrane segments between residues 483 and 647 (Figure 2B and C). The position of the transmembrane segments was equally detected by programs PSORT, PredictProtein, Tmpred and PeptideStructure. PSORT data suggested at a probability of 64% that AtPIN2p was a type‐III membrane protein anchored through internal sequences in the plasma membrane (Singer, 1990).
The Atpin2::En701 mutants exhibit agravitropism and altered auxin physiology
For functional analysis of the AtPIN2 gene, we screened for a knock‐out mutant in a collection of Arabidopsis plants mutagenized by the maize transposon En‐1 (E.Baumann, J.Lewald, H.Saedler, B.Schultz and E.Wisman, submitted; Wisman et al., 1998). We identified a mutant, Atpin2::En701, in which the En‐1 transposon was inserted into the second exon of the AtPIN2 gene (between nucleotide positions 701 and 702) thereby disrupting the codon for Leu151 (Figure 1).
To characterize the Atpin2::En701 mutant with respect to growth of aerial and root organs, homozygous progeny were grown on agar plates. Roots of mutant plants displayed an agravitropic phenotype coupled to reduced root elongation growth (Figure 3A and B). Physical mapping revealed that the chromosomal position of AtPIN2 corresponded to the WAV6 locus. Since the wav6‐52 EMS mutant exhibited a similar agravitropic phenotype as Atpin2::En701 (Okada and Shimura, 1990), we performed crosses between the homozygous wav6‐52 and Atpin2::En701 mutants to test for allelism. Analysis of the F1 progeny indeed showed that wav6‐52 and Atpin2::En701 were allelic (data not shown). We have tested the auxin sensitivity of root elongation for the Atpin2::En701 and wav6‐52 alleles, since several agravitropic mutants have been observed to exhibit altered auxin response (Hobbie and Estelle, 1995; Simmons et al., 1995). Seeds were first germinated on hormone‐free Murashige and Skoog (MS) agar medium, then transfered onto MS agar plates containing various concentrations of 1‐naphthylacetic acid (1‐NAA; Figure 4A and B), indole‐3‐acetic acid (IAA; Figure 4C and D) or 2,4‐dichlorophenoxyacetic acid (2,4‐D; Figure 4E and F). Additional root growth following transfer was measured after 3 days and the percentage of root elongation was calculated relative to growth on media containing no auxin. The results revealed that elongation of Atpin2::En701 mutant roots was more sensitive than wild‐type at elevated concentrations of 1‐NAA (p <0.0001 student t‐test at 3.3×10−7 M; Figure 4A), and IAA (p <0.0001 Student's t‐test at 10−7 M; Figure 4C), but no difference in 2,4‐D sensitivity was found between them (Figure 4E). Similar responses have been observed for the wav6‐52 allele (Figure 4B, D and F). The greater sensitivity of Atpin2::En701 and wav6‐52 mutant roots towards the auxins 1‐NAA > IAA > 2,4‐D (Figure 4) is consistent with the known substrate specificity of the auxin efflux carrier (Delbarre et al., 1996).
Expression analyses revealed a polar localization of AtPIN2p in root cells
Northern blot analysis showed that AtPIN2 was expressed in young seedlings and roots of plants grown on agar and in liquid culture, respectively (Figure 5A). This was confirmed by Western blot analyses using affinity‐purified polyclonal anti‐AtPIN2p antibodies raised against a polypeptide extending from residues 275–487 of AtPIN2p. The anti‐AtPIN2p antibodies detected a specific signal corresponding to the expected size of 69 kDa in membrane fractions of Arabidopsis roots (Figure 5B). In order to localize AtPIN2 expression, the spatial distribution of its mRNA was determined by whole‐mount in situ hybridization using 3‐day‐old seedlings (Figure 6). AtPIN2 expression was observed in a region spanning meristematic and distal elongation zones of wild‐type seedlings (Figure 6), whereas no signals were detected in the Atpin2::En701 mutant (data not shown). To localize the AtPIN2 protein within root cells, whole‐mount in situ immunostaining analysis of 4‐day‐old seedlings was performed using affinity purified anti‐AtPIN2p primary antibodies with fluorescein isothiocyanate (FITC)‐coupled secondary antibodies. Immunohistochemical staining of wild‐type plants revealed AtPIN2p‐specific signals in a region spanning meristematic and distal elongation zones, consistent with the AtPIN2 mRNA in situ hybridization results (Figure 7). In roots of homozygous Atpin2::En701 mutant plants we observed only diffuse background staining as expected for a loss‐of‐function mutant (Figure 7C). In wild‐type seedlings, the AtPIN2p‐specific FITC fluorescence was localized exclusively in membranes of root cortical and epidermal cell files, starting three to five cells distant from the quiescent center (Figure 7B and D). No signal was observed in the stele, pericycle or endodermis. The fluorescence was very weak, if not completely absent, at the periclinal side of cortical cells contacting the endodermal cells. Stronger signals were observed at the anti‐ and periclinal sides of cortical cells adjacent to other cortical and epidermal cells (Figure 7B, D and E). In the epidermal cell file AtPIN2p was preferentially localized to the anticlinal side of the cells (Figure 7D and E). As the epidermal cell file extended towards the elongation zone, the signal was present at the basal side of cells oriented towards the elongation zone (Figure 7F). This striking cellular polarity of AtPIN2p is highly reminiscent of the basal localization of the homologous AtPIN1 protein in Arabidopsis inflorescence axes (L.Gälweiler, C.Guan, A.Müller, E.Wisman, K.Mendgen, A.Yephremov and K.Palme, submitted). This common basal localization of AtPIN1 and AtPIN2 proteins in different auxin‐transport‐competent cells is consistent with the proposed direction of auxin flux in each of these tissues (Lomax et al., 1995).
The mechanisms underlying gravitropic growth have been intensively investigated since Cholodny and Went independently proposed their elegant models, leading to the formulation of the Cholodny–Went hypothesis (Evans, 1991; Estelle, 1996). This hypothesis predicts that gravitropic curvature of plant organs results from redistribution of the plant hormone auxin. By differential transport processes, auxin accumulates at one side of the organ thereby affecting the differential elongation growth at one side of the tissue causing directional curvature (Harrison and Pickard, 1989; Young et al., 1990). Direct demonstration of a gravity‐induced auxin gradient in roots has been problematic, mainly due to artifacts which can occur when radioactively labeled auxin is applied exogenously to roots (Pernet and Pilet, 1976) and the lack of better analytical techniques.
To study the mechanisms involved in gravitropism control, mutants altered in their response to gravity provide important tools. Thus, several mutants impaired in distinct steps of gravitropic control have been isolated. Interestingly, most of these mutants were derived from genetic screens for auxin resistance (Hobbie and Estelle, 1995; Bennett et al., 1996), suggesting a strong link between defects in root auxin physiology and control of gravitropism. Although most available mutants still require a detailed functional characterization, physiological experiments using roots incubated with either exogenous auxin or polar auxin transport inhibitors such as N‐1‐naphthylphthalamic acid (NPA) have demonstrated that auxin transport plays an essential role in the regulation of gravitropism. Roots of some agravitropic mutants, such as axr4 (rgr1), are resistant to natural and synthetic auxins like IAA and 2,4‐D as well as to polar auxin transport inhibitors (Hobbie and Estelle, 1995; Simmons et al., 1995).
In our study, we describe the isolation and characterization of an Arabidopsis mutant, Atpin2::En701, and the corresponding AtPIN2 gene. We demonstrate that this agravitropic mutant displays reduced root elongation growth. Reduced root elongation of Atpin2::En701, even on medium without exogenous auxin, mimics the effect of high auxin concentrations or polar auxin transport inhibitors such as NPA on wild‐type root growth (Muday and Haworth, 1994), consistent with a lack of auxin efflux carrier function in cortical and epidermal cell files. Furthermore, NPA causes agravitropic root growth as has been observed for Atpin2::En701 and several other auxin‐transport‐deficient mutants (Muday and Haworth, 1994; Bennett et al., 1996; Garbers et al., 1996). The altered auxin response of Atpin2::En701 roots and the agravitropism can therefore be rationalized as a result of a defect in auxin transport processes.
The predicted structure of the AtPIN2 protein suggests that it performs a transport function, sharing a similar topology with carrier proteins from the major facilitator superfamily, a widely conserved class of transport proteins in bacteria and eukaryotes. These transporter proteins typically contain 12 transmembrane domains which are interrupted by a central and apparently dispensable hydrophilic loop resulting in a 6 + 6 transmembrane segment pattern. Members of this transporter superfamily catalyze the transport of a wide variety of drugs such as quinolone or tetracycline, sucrose, lactose, arsenic acid and organic phosphate esters (Marger and Saier, 1993; Diorio et al., 1995; Hoenke et al., 1997).
As a member of the PIN gene family, AtPIN2p shares sequence and topology similarity with AtPIN1p. Mutations within the AtPIN1 gene result in a drastically reduced level of basipetal auxin transport within the inflorescence tissues, leading to the proposal that the AtPIN1 protein plays an important role in the control of cellular auxin efflux (L.Gälweiler, C.Guan, A.Müller, E.Wisman, K.Mendgen, A.Yephremov and K.Palme, submitted; Goto et al., 1991; Okada et al., 1991). Mutations within AtPIN2 confer an auxin‐sensitive phenotype which is consistent with a loss of auxin efflux carrier function in root cells (Figure 4). We rationalize that exogenous auxin will accumulate within elongating root cells in the absence of an auxin efflux carrier, resulting in an auxin‐sensitive phenotype and subsequently causing inhibition of root growth. Furthermore, Atpin2 mutant roots exhibit greater sensitivity towards the auxins 1‐NAA > IAA > 2,4‐D (Figure 4), which is consistent with the known substrate specificity of the auxin efflux carrier (Delbarre et al., 1996). The characteristics of the Atpin2 mutant contrast with that described for the putative auxin influx carrier mutant aux1 which confers reduced auxin sensitivity, consistent with an impaired ability of the mutant to accumulate inhibitory levels of auxin (Bennett et al., 1996).
Auxin is unique amongst plant hormones in demonstrating a polarity in its movement (Goldsmith, 1977). Rubery, Sheldrake and Raven have proposed within their chemiosmotic hypothesis that the polarity of IAA transport reflects the asymmetric subcellular distribution of auxin efflux (and possibly auxin influx) carrier proteins (Rubery and Sheldrake, 1974; Raven, 1975).
The AtPIN1 protein has been localized to the basal side of auxin‐transport‐competent cells within root and shoot vascular tissues (L.Gälweiler, C.Guan, A.Müller, E.Wisman, K.Mendgen, A.Yephremov and K.Palme, submitted). The cellular immunolocalization of AtPIN2p revealed a basal distribution within epidermal root cells in the meristematic and the elongation zone (Figure 7). In the epidermis, auxin is thought to be transported basipetally from meristematic tissues towards the elongation zone of roots (Ohwaki and Tsurumi, 1976; Lomax et al., 1995). The asymmetric cellular localization of AtPIN1p in inflorescence tissues (L.Gälweiler, C.Guan, A.Müller, E.Wisman, K.Mendgen, A.Yephremov and K.Palme, submitted) and AtPIN2p in the root epidermis is consistent with a role in mediating directional auxin fluxes within these cell types.
The transporter‐like topology of the AtPIN2 protein, its homology to AtPIN1p as well as to several other transporters, the remarkable polarized localization of AtPIN2p in root cells and the Atpin2 mutant phenotype suggest that AtPIN2p influences root gravitropism by functioning as a transmembrane component of the auxin efflux carrier complex. Morris et al. (1991) proposed that the auxin efflux carrier complex consists of at least three components: a transmembrane carrier protein, an NPA‐binding protein and a third component of high turnover. Ruegger et al. (1997) have described the isolation of the tir3 mutant which exhibits significantly reduced levels of NPA binding, leading to the suggestion that TIR3 may encode (or regulate the activity of) the NPA‐binding protein associated with the efflux carrier. AtPIN2p is likely to represent the transmembrane component of the auxin efflux carrier complex.
In summary, our results have led us to formulate a model featuring AtPIN2p regulating directional auxin efflux in cortical and epidermal root cells. As depicted schematicaly in Figure 8, auxin, supplied from the shoot to the root tip via the stele into cortical and epidermal cells, is basipetally transported backward towards the elongation and differentiation zone by AtPIN2p (Lomax et al., 1995; Estelle, 1996). This hypothesis is supported by various physiological measurements in maize roots which demonstrate the existence of such basipetal auxin transport (Pernet and Pilet, 1976; Martin et al., 1978). We propose that the loss of AtPIN2p function impairs basipetal auxin transport, hence disrupting the response of the elongation zone upon gravistimulation.
Materials and methods
Plant material and growth conditions
Seeds of Arabidopsis thaliana Heynh., ecotype Columbia (Col‐O) or Landsberg erecta (Ler), were grown on soil under white light in a controlled environment chamber (irradiance 150 μE/m2/s, 16 h light, 60% humidity, 20–25°C day temperature, 10–15°C night temperature). For in vitro culture, seeds were surface sterilized using 5% sodium hypochloride and plated on agar MS medium (4.3 g/l MS salts, 1–3% sucrose, 1% bactoagar pH 6.0 with 1 M KOH; Murashige and Skoog, 1962). To break dormancy, seeds were incubated for 1–2 days at 4°C. The hormones NAA and IAA were dissolved in 1.0 M NaOH, whilst 2,4‐D was resuspended in 100% dimethyl sulfoxide (DMSO). The resuspended hormones were diluted with water (30:70) to provide stock solutions which were later added at various dilutions to plant growth media.
Auxin resistance assays
Seedlings were grown on MS agar in constant white light at 22°C for 3 days. Seedlings of similar size were transferred to MS agar containing various concentrations of auxins and the position of primary root tips was marked. The seedlings were grown vertically for 3 days under the same growth conditions, then additional root growth was measured. The percentage growth relative to the control growing on media with no auxin addition was calculated.
The Atpin2::En701 mutant was identified by reverse genetic screening of an En‐1 mutagenized collection of Arabidopsis plants. PCR‐based screens were performed according to a three dimensional grid as described (Baumann et al., submitted). The following primers specific for the AtPIN2 gene were used: P2‐En4: 5′‐GAAGAAAAGGGCATGTGGAAA‐3′; P2‐En5: 5′‐CCTCTGGTCATAGCATTCTT‐3′.
General cloning procedures and database searches
All cloning procedures were performed according to standard methods (Ausubel et al., 1994). Arabidopsis genomic and cDNA libraries were screened using a probe derived from the AtPIN1 gene (DDBJ/EMBL/GenBank accession No. AF089084; Gälweiler et al., submitted). Sequencing was performed using an ABI sequencer (377) according to the manufacturer's instruction. Sequences were aligned and analyzed using Gap, Map and Pileup programs of the GCG program package (version 8; Genetics Computer Group, Madison, WI; Devereux et al., 1984). The nucleotide and predicted amino acid sequences were compared with the database using BLASTN and TFASTA algorithm (Altschul et al., 1990; Pearson, 1990). Oligonucleotides were designed using the Oligo4S program and obtained from commercial suppliers (MWG, Ebersbach). For polypeptide topology prediction, the following servers were used: http://psort.nibb.ac… (Psort; Nakai and Kanehisa, 1992), http://www.embl-heidelberg.de/predictprotein/predictprotein.h… (PHD topology; Rost, 1996), http://ulrec3.unil.ch/software/TMPRED_form.html (Tmpred; Hofmann and Stoffel, 1993) and Peptidestructure (GCG program package; Devereux et al., 1984).
Arabidopsis plants were grown either in vitro on agar plates for 3 days, in liquid culture for 2 weeks or in the soil for 3 weeks. Organs were collected, frozen in liquid nitrogen and stored at −80°C. Isolation of mRNA and Northern blot hybridizations were performed as described (Teichmann et al., 1997). Expression of RPL4 (ribosomal protein large subunit 4; Kim et al., 1990) was used to monitor uniformity of loading of RNA.
In situ hybridization
A region of AtPIN2 cDNA corresponding to nucleotides 841–1461 was cloned in pBluescript and used as a probe. Digoxigenin‐labeled sense and antisense probes were prepared by in vitro transcription (Boehringer Mannheim). Three‐day‐old seedlings were fixed, permeabilized and hybridization performed as described (Ludevid et al., 1992). After hybridization, the samples were washed once with 4× SSC for 10 min at 20°C, 2× SSC for 30 min at 20°C, and then with 0.2× SSC at 65°C for 30 min.
Immunolocalization and Western blot analysis
To generate polyclonal AtPIN2p‐specific antibodies, a PCR fragment carrying the cDNA sequence coding for a peptide between amino acids 275–487 was synthesized and inserted into pT7H6(GS)2 (Studier et al., 1990). Expression of the recombinant protein containing an N‐terminal His6 tag was performed in Escherichia coli BL21 after induction with CE6 phages. The 24 kDa recombinant protein was extracted from bacteria, purified by Ni2+–NTA chromatography and used for immunization of rabbits. The rabbit polyclonal anti‐AtPIN2p antiserum was affinity purified according to standard procedures (Ausubel et al., 1994) and analyzed by Western blotting using the recombinant antigen and Arabidopsis microsomal fractions. Microsomal fractions of Arabidopsis roots from plants grown in liquid medium or on agar plates were isolated by differential centrifugation (Zettl et al., 1994), incubated in SDS–PAGE sample buffer for 5 min at 42°C, and separated by electrophoresis on 10% SDS–PAGE gels. Proteins were electrotransferred to PVDF membranes, the blots were blocked with phosphate‐buffered saline/4 % low fat milk powder and incubated with anti‐AtPIN2p antibodies. Signal detection was achieved using peroxidase‐conjugated secondary antibodies and by performing chemiluminescence reaction (ECL kit; Pierce) followed by X‐ray film exposure.
Four‐day‐old Arabidopsis seedlings were incubated for 1 h under vacuum in 50 mM PIPES, 5 mM EGTA, 5 mM MgSO4 pH 7.0 (MTSB). Seedlings were subsequently washed three times for 15 min in MTSB/0.1% Triton X‐100, three times in water/0.1% Triton X‐100 and then incubated in MTSB/2% Driselase (Sigma, Deisenhofen) for 15–30 min. After washing three times with MTSB, seedlings were permeabilized by incubation in 0.5% nonident P‐40/10% DMSO in MTSB for 1 h, washed again three times with MTSB and incubated in blocking buffer (3% bovine serum albumin/MTSB) for 30 min. Permeabilized seedlings were incubated with affinity‐purified anti‐AtPIN2p antibodies in a humid chamber for 1 h, washed three times for 5 min in MTSB and further incubated for 1–2 h at 20°C with FITC‐conjugated anti‐rabbit IgG antibodies (Boehringer Mannheim) diluted in blocking buffer. Before mounting, seedlings were washed three times for 5 min with MTSB followed by washing twice with water. Fluorescent signal detection and documentation was performed with the help of a confocal laser scanning microscope (Leica DMIRBE, TCS 4D with digital image processing) using a 530 ± 15 nm band‐pass filter for FITC‐specific detection and a 580 ± 15 nm band‐pass filter for autofluorescence detection. For histological signal localization, both images were electronically overlayed resulting in a red autofluorescence and green–yellow AtPIN2p‐specific fluorescence. Images were further processed by Photoshop 4.0 for graphic presentation.
Note added in proof
During revision of this manuscript, Lusching et al. reported the isolation and characterization of the Arabidopsis EIR1 gene (Genes Dev., 12, 2175–2187). The agravitropic mutation eirl‐3 was shown to be allelic to the previously described mutations wav6‐52/agr1.
The authors wish to thank Mrs E.Baumann for expert technical assistance during part of the work, the ADIS group for DNA sequence analysis, Dr Renate Schmidt for providing YAC contig data and Dr C.Koncz for critical evaluation of the manuscript. This work was supported by research grants provided by the European Communities's BIOTECH programme (PL96‐0487) and the Arabidopsis programme of the DFG.
- Copyright © 1998 European Molecular Biology Organization