The Eps homology (EH) domain is a recently described protein binding module that is found, in multiple or single copies, in several proteins in species as diverse as human and yeast. In this work, we have investigated the molecular details of recognition specificity mediated by this domain family by characterizing the peptide‐binding preference of 11 different EH domains from mammal and yeast proteins. Ten of the eleven EH domains could bind at least some peptides containing an Asn‐Pro‐Phe (NPF) motif. By contrast, the first EH domain of End3p preferentially binds peptides containing an His‐Thr/Ser‐Phe (HT/SF) motif. Domains that have a low affinity for the majority of NPF peptides reveal some affinity for a third class of peptides that contains two consecutive amino acids with aromatic side chains (FW or WW). This is the case for the third EH domain of Eps15 and for the two N‐terminal domains of YBL47c. The consensus sequences derived from the peptides selected from phage‐displayed peptide libraries allows for grouping of EH domains into families that are characterized by different NPF‐context preference. Finally, comparison of the primary sequence of EH domains with similar or divergent specificity identifies a residue at position +3 following a conserved tryptophan, whose chemical characteristics modulate binding preference.
The dynamic assembly of macromolecular complexes inside the cell is often mediated by relatively small protein recognition modules that are widespread in several proteins (Pawson and Scott, 1997). Each module family binds relatively short peptides with different chemical or structural characteristics. For instance, SH2 and PTB bind phosphotyrosine peptides, while SH3 domains require the target peptide to be folded into a proline type II helix. Within families, the recognition specificity of each member rests on subtle chemical variations, on a common structural theme, of the recognition domains that are reflected by complementary variations on the target peptide (Songyang et al., 1993, 1994, 1997; Rickles et al., 1994; Sparks et al., 1994; Dente et al., 1997).
EH is a recently described protein recognition domain that was first identified as a 100 amino acid module repeated three times in the N‐terminus of the epidermal growth factor (EGF) receptor substrate Eps15, and of the related protein Eps15R (Fazioli et al., 1993; Wong et al., 1995; Di Fiore et al., 1997). Searching the protein database for similar peptide sequences revealed that EH domains are found in several proteins in species as diverse as yeast and humans. When functional information is available, EH‐containing proteins are often associated with regulation of protein transport/sorting and membrane trafficking. Eps15 and Eps15R are both components of clathrin‐coated pits, and co‐localize with AP2 (Tebar et al., 1996; van Delft et al., 1997; Coda et al., 1998) and with synaptojanin 1 in coated endocytic intermediates in nerve terminals (Haffner et al., 1997). In vitro, their C‐terminal DPF‐rich domain has been shown to be essential for binding to the so‐called ‘ear’ of α‐adaptin (Benmerah et al., 1996; Iannolo et al., 1997). It was recently demonstrated that Eps15 is an essential component of the endocytic machinery, since endocytosis of EGF and transferrin can be blocked by anti‐Eps15 antibodies or by over‐expression of protein fragments encompassing either the N‐terminal EH domain or the C‐terminal DPF domain (Carbone et al., 1997; Benmerah et al., 1998). Furthermore, intersectin, or its Drosophila homolog Dap160, which contain two EH domains, bind to dynamin, another molecule whose participation in coated pits‐mediated endocytosis is clearly established (Roos and Kelly, 1998; Yamabhai et al., 1998). Yeast strains mutated in two of the three genes encoding EH‐containing proteins, PAN1 and END3, are defective in endocytosis (Benedetti et al., 1994; Munn et al., 1995; Tang et al., 1997; Wendland and Emr, 1998).
Actin cytoskeleton regulation is another function linked to EH‐containing proteins, as shown by the involvement of Pan1p and End3p in the organization of the actin cytoskeleton (Tang and Cai, 1996; Tang et al., 1997). Recently, an EH‐containing protein was found that binds RalBP1, a GTPase activating protein for CDC42 and Rac GTPases (Yamaguchi et al., 1997; Ikura et al., 1992).
It is assumed that the peptide recognition specificity of EH domains plays an important role in determining the biological properties of EH‐containing proteins, possibly by modulating the formation of macromolecular complexes. By screening a nonapeptide repertoire displayed by fusion to the major coat protein of filamentous phage (Felici et al., 1991), we have found that the N‐termini of Eps15 and Eps15R bind peptides that contain an NPF (Asn‐Pro‐Phe) motif (Salcini et al., 1997). Similarly, the EH domains of intersectin bind linear or constrained NPF‐containing peptides (Yamabhai et al., 1998). Indeed Eps15‐binding partners such as Rab, Numb, RabR, NumbR and synaptojanin contain single or multiple NPF motifs that, at least in the case of Numb, mediate in vivo recognition. Although only the NPF tripeptide was found to be essential for binding, alanine scanning mutagenesis of peptide targets suggested that positions +1, −1 and −2 with respect to NPF also contribute to modulate binding affinity (Salcini et al., 1997). The following studies were undertaken to investigate the molecular basis of EH recognition specificity.
The six EH domains of Eps15 and Eps15R display diverse recognition preferences
The six EH domains of Eps15 and Eps15R were expressed by fusing their coding sequences to the glutathione‐S‐transferase (GST) gene in pGEX expression vectors. The purified hybrid proteins were used to screen a multivalent nonapeptide phage display library as previously described (Felici et al., 1991; Dente et al., 1997). In each panning experiment, after two panning cycles ∼20 single clones were tested by phage ELISA, and those confirmed to be positive for binding were sequenced. The selected peptides (Figure 1) indicate that each individual EH domain from Eps15 and Eps15R can bind to NPF‐containing peptides. However, from the comparison of the peptide sequences in each series, it is possible to recognize a certain degree of recognition specificity. Both the first and third EH domains of Eps15R (EH1R and EH3R, respectively), with a single exception, selected peptides that display an R after the conserved NPF motif. Furthermore, EH3R displays a significant preference for Q at position +2. These results are consistent with the observation that Eps15R, but not Eps15, binds preferentially to NPFR peptides (Salcini et al., 1997). In contrast, the first two EHs of Eps15 (EH1 and EH2) and the second of Eps15R (EH2R) are less selective and tolerate several residues at position +1. Small hydrophilic residues such as T, N or S are found preferentially at position −1 in most of these domains.
The behaviour of the third domain of Eps15 (EH3) is strikingly different. Although two of the peptides selected by this domain contain the NPF motif, the majority is characterized by the presence of FW (Phe‐Trp). Since one peptide contains the sequence NPFW, it is possible that the FW dipeptide is part of an extended NPFW consensus.
To obtain direct evidence that different EH domains have distinct preferences when challenged with different peptides we selected 10 EH‐binding phage clones and measured their ability to bind to the EH domains by ELISA type assay (see Materials and methods). The results shown in Figure 2 support the notion that different domains preferentially recognize NPF peptides within specific contexts. EH2R is the less selective among the tested domains and binds to most NPF peptides with comparable efficiency. EH1 and EH2 display a similar pattern with somewhat reduced affinity. In agreement with the consensus sequences derived in Figure 1, EH1R and EH3R are highly selective for peptides with an R after the NPF consensus while EH3 hardly binds to NPF peptides, with the possible exception of NPFL and NPFW, and is the only domain that binds to FW‐containing peptides efficiently.
Qualitatively, the binding properties of the Eps15 and Eps15R fragments containing the three EH domains can be looked at as the sum of the binding properties of their constituent domains, suggesting that in the native protein, each domain is available for binding to its favourite target.
The diverse recognition specificity of the EH domains of Eps15 were further confirmed in experiments in which synthetic peptides were presented to the domains outside the phage capsid context (Figure 3).
Binding specificity of yeast EH domains
EH domains are found in proteins of many species, from lower eukaryotes to mammals. We have used the PSI_BLAST program to search the entire database for proteins containing single or multiple EH domains (Figure 4). As the entire nucleotide sequence of Saccharomyces cerevisiae is now available, it is possible to identify the entire ‘EH repertoire’ of a simple eukaryotic organism. Two reiterations of the PSI_BLAST program with an E‐value of 10−4, and utilizing as a bait the EH1 domain of Eps15, identified five yeast proteins with an E‐value above the threshold. Starting the search with a different EH domain did not alter the results substantially. Two of the yeast EH‐containing proteins, YBL047cp and Pan1p, contain multiple EH domains, three and two, respectively. The remaining three proteins, End3p, YKR019cp and YJL083wp, display a single EH domain. A second divergent EH domain could be identified in End3p at a lower stringency and was therefore included in our analysis (E‐value of 0.065 after two iterations with an E‐value of 0.001).
The nine EH‐domain coding sequences were cloned into pGEX expression vectors and the corresponding fusion proteins were purified by affinity chromatography. In the case of YKR019cp and YJL083wp, we have not been able to purify sufficient hybrid protein, possibly due to protease degradation. Thus, these two domains were not investigated further. The seven EH domains of the remaining three proteins were utilized in panning experiments, and with five of them we were able to identify target peptides after two or three selection cycles (Figure 5).
The first EH domain of YBL047cp binds preferentially to peptides displaying a (F/L)WR consensus, reminiscent of the FW consensus recognized by the EH3 domain of Eps15. However, one peptide was found that contains an NPF motif. NPF is also one of the consensus sequences derived from the peptides selected by the second EH domain, the other being WWxxad. Finally, the third YBL047cp EH domain has preferences for NPFR‐containing peptides.
NPF peptides could also be selected by panning with the second domain of Pan1p. However, with this domain, four panning cycles were necessary to enrich for binding peptides, and the eight phages that were characterized were found to display only three different peptides. As confirmed later by phage ELISA (not shown), this suggests that the selected phage binds poorly to the Pan1p‐EH2 domain. By contrast, the first EH domain of Pan1 did not select any phage in panning experiments. Furthermore, a GST‐fusion protein containing both the first and second domain of Pan1p bound to phages that were indistinguishable from those selected by EH2 alone (not shown).
H(S/T)F is the somewhat unorthodox consensus identified by peptides selected by the EH1 domain of End3p. Conversely, we could not enrich for any specific binding peptide after four panning cycles with the second EH domain of this protein. Also in this case, panning with an End3p protein fragment containing both domains resulted in selection of peptides very similar to those selected by EH1 alone (not shown). When the EH1 domain of End3p was used to pan a phage library displaying pentadecapeptides a different consensus could be deduced sWGxxxw.
The three peptide classes, selected by the second domain of Pan2p and by the first of End3p, can be aligned without shifting their frame, suggesting that one or more residues in the coat protein context may be important in modulating binding affinity. The coat residues that might contribute to binding are indicated in italic in Figure 5.
The C‐terminal boundary of the EH domain
By searching the database of proteins whose three‐dimensional structure has been determined (Protein Data Bank), with a protein profile derived from an alignment of EH domains, a significant homology was detected between a portion of the EH domain and proteins that display an EF‐hand fold, such as calmodulin, recoverin and sarcoplasmic binding protein. The structural significance of this homology has been confirmed recently by the determination of the solution structure of the second EH domain of Eps15 (de Beer et al., 1998). The N‐terminal 70‐80 residues of EH domain fold into two helix‐loop‐helix motifs connected by a short antiparallel β‐sheet. The C‐terminal 25 residues that are included in our standard constructions display a conserved pattern of repeated hydrophobic and proline residues that, in the three‐dimentional structure, zigzag over the third and fourth helix (Figure 4). However, the C‐terminal boundaries of a functional EH domain cannot be unequivocally identified by sequence alignment due to variable amino acid conservation, which is lower than in the N‐terminal portion.
In order to examine whether the C‐terminal regions of EH are necessary for peptide binding, we expressed derivatives of the three domains of Eps15 and the first domain of End3p, and tested their ability to bind to a set of target peptides by phage ELISA. We have observed that a deletion of ∼25 residues, including the hydrophobic‐Pro motif, in any of the three Eps15 domains completely abolishes binding (Figure 6). Thus, we conclude that the core EH domain containing only the two helix‐loop‐helix motifs is not sufficient for NPF peptide binding.
Similarly, the first domain of End3p requires a C‐terminal region that extends beyond the EF‐hand homology boundary since an End3p that terminates at residue K96 does not show any detectable binding to phages displaying various HTF or HSF peptides (Figure 6).
Residues that are involved in peptide binding
The residues corresponding to L165 and W169 in the Eps15‐EH2 sequence are among the most conserved in the EH domain family. We therefore tested whether these residues are involved in ligand binding. We constructed four mutant Eps15‐EH2 domains by changing L165 into A, and W169 into Y, F or A. All the mutant domains seem to fold properly, judging by the yield of soluble GST‐EH hybrid protein obtained in overproducer strains. However, both L165A and W169A have completely lost their ability to bind NPF‐containing peptides (Figure 7). In contrast, when W169 is changed into either F or Y, most of the binding activity is retained.
Two classes of peptides
The EH‐domain ligands, as deduced from our panning experiments, can be grouped into two different classes. Most EH domains select peptides that contain a variation of the typical NPF motif. In contrast, some domains like Eps15‐EH3, the first and second domain of YBL047cp, or the first of End3p, select peptides that are characterized by consensi containing aromatic and hydrophobic residues (FW, WW, SWG, etc.) (Figures 1 and 5). We term the latter class II peptides. Notably, some of the domains with a marked preference for NPF peptides, such as Eps15‐EH1, also displayed some affinity for class II peptides (not shown).
The third EH domain of Eps15 selects both NPF‐containing (class I) and FW‐containing (class II) peptides, thus representing a good model to test whether the two different classes of peptides bind to the same or different sites (Figure 1). Using competition experiments, we tested the ability of NPF and FW peptides to bind to the EH3 domain of Eps15. As shown in Figure 8, the synthetic GSTPGQVAFWDP peptide is equally efficient (IC50 ≈ 50 μM) in competing the binding of EH3 to either the cognate FW peptide (GSTPGQVAFWDP) or the NPFA (GSGSLWSSTNPFAD) and NPFW (GSMRNRANPFWDP) peptides. The same FW peptide, however, cannot compete with an NPFR peptide (GSAKTNPFRQQD) for binding to the EH3 domain of Eps15R, consistent with its inability to bind to this domain. Similarly, the NPFW peptide competes with the same efficiency (IC50 ≈ 200 μM) with binding of EH3 to both peptide classes This result strongly suggests that the binding sites for the two peptide classes are either coincident or very close.
A residue that modulates binding specificity
By comparing the primary sequences of domains with similar or divergent recognition specificity we have identified a residue, at position 172 (+3 with respect to the conserved tryptophan), whose chemical characteristics correlate with recognition specificity (see Figure 4 and Discussion). Domains that prefer NPF have an Ala or Ser at this position (Ala being preferred by those requiring an Arg after the NPF motif), while the domains that bind to class II peptides have slightly larger side chains, either Cys or Val.
To test whether this residue could modulate recognition specificity, we have constructed four derivatives of EH3 and EH3R by replacing the Cys that is present in EH3 at this position with either an Ala, or a Ser and the Ala of EH3R into either Cys or Val. We have then assayed, by phage ELISA, the ability of these mutants to bind to a sample of NFP or FW peptides.
The results reported in Figure 9 support our prediction, since EH3 domains that have Cys replaced by either Ala or Ser bind more efficiently to NPF‐containing peptides, while EH3 domains with Cys and Val acquire the ability to bind to FW and NPFW peptides.
The observed change in recognition patterns, however, cannot be simply described as a shift from NPF to FW specificity, but rather as a relaxation of recognition specificity. EH3 C→A, for instance, acquires the ability to bind to NPFR peptides but retains the ability to bind FW peptides. Conversely, EH3R A→C (or A→V) maintain their ability to bind NPFR peptides, albeit with somewhat reduced affinity.
Previous work (Salcini et al., 1997) had shown that the N‐termini of Eps15 and Eps15R, containing three 100‐amino‐acid repeats (EH domains), bind peptides that share a common NPF motif. Furthermore, it was concluded that binding of Eps15 to the protein NUMB in vivo is mediated by binding of the EH domain to an NPF tripeptide located near the C‐terminus (Salcini et al., 1997). Recently, we have found that the EH domains of intersectin also bind to NPF peptides (Yamabhai et al., 1998).
However, the molecular basis of recognition of specific peptides by individual EH domains remained to be elucidated. In this study we demonstrate that a large fraction of EH domains recognises NPF peptides albeit with a different sequence context preference.
Panning a phage‐displayed nonapeptide library with 13 different EH domains revealed that at least 10 can bind peptides containing the NPF motif. Among the remaining three, the first EH domain of the yeast protein Pan1p and the second of End3p could not find any specific target structure in our peptide library.
The first EH domain of End3p, in contrast, binds to peptides that share the HTF/HSF tripeptide consensus, thus defining a new EH domain recognition specificity. We considered the possibility that NPF and HTF/HSF peptides could share a common conformation, despite the sequence difference. Thus, we compared the conformation of the NPF or H(T/S)F peptides that are present in the proteins of known structure in the PDB database. We observed, however, that while a large fraction of NPF share a common turn‐like conformation, stabilized by a conserved pattern of hydrogen bonds, HTF peptides have a much wider distribution of conformations (not shown).
When all the selected NPF peptides are considered together, no strong preference can be identified in the position immediately preceding or following the NPF motif. However, the preference for Asn and Thr at position −1, Arg at position +1 and Gln at +2, is statistically significant, with an occurrence that is approximately four times higher than predicted by the respective codon frequency. More striking is the strong negative bias for some residues that are never found at specific positions. For instance, negatively charged residues are never found at positions −1 and +1. Ile, Val and Trp are also not tolerated at −1, while Ile and Tyr are heavily under‐represented at any position following NPF.
However, by grouping NPF binding domains into broad families, further regularities can be identified. There are domains belonging to the first family, such as the first and second domain of Eps15R or the third domain of the yeast protein YBL047c, which display a strong preference for R at position +1. The peptides selected by domains of this first group also display a statistically significant bias for positive residues at position −2 and −3, and for glutamine and alanine at position +2.
A second family of EH domains, including the first and second domain of Eps15 and the second domain of Eps15R, is less selective, showing only a slight preference (∼3‐fold) for Thr at position −1 and Ala at +1.
Finally, the last family possesses domains, such as the first and second domain of YBL047cp, the third domain of Eps15, or the second domain of Pan1p, which generally have a lower affinity for NPF peptides and show a marked preference for Asn at −1 and for Trp at +1. An additional characteristic of this last domain family is the ability to select another class of peptides (class II) that does not contain NPF and is characterized by two aromatic residues: FW in the case of YBL047cp‐EH1 and Eps15‐EH3, and WW for YBL047cp‐EH2. In addition, the End3p‐EH1 selects, aside from HTF peptides, a second peptide family containing a Trp (SWG peptides).
The functional significance of the NPF consensus, identified by phage display, has been demonstrated by the isolation of several EH‐binding proteins containing one or more NPF motifs (Haffner et al., 1997; Salcini et al., 1997; Yamabhai et al., 1998), and by the observation that the C‐terminal motif of the protein NUMB is essential for NUMB‐Eps15 co‐immunoprecipitation (Salcini et al., 1997). It is not clear at present whether class II peptides, displaying the consensus with two aromatic residues, would also represent a second functionally relevant binding mode of EH domain or if they should be considered as mimotopes.
The consensus sequences that we have determined can be used as bait to search protein databases with a pattern search algorithm for putative protein targets. In S.cerevisiae, where the entire genome sequence has been determined, this search can be exhaustive (Chervitz et al., 1997). In a typical search one may retrieve from a few to a few hundred putative targets, depending on the stringency of the pattern utilized in the search (not shown). Some judicious selection, for instance disregarding the putative target proteins that are known to be in a different cellular compartment to the bait, allows us to focus on a sufficiently low number of candidates to be approached experimentally. Recently, Wendland and Emr (1998) reported that the region encompassing the two EH domains of Pan1p binds yAP180Ap, one of the two yeast homologues of a class of clathrin assembly proteins (AP180). The same authors identified a genetic interaction between PAN1 and SJL1, one of the three synaptojanin‐like genes in yeast. Interestingly, yAP180Ap contains five NPF motifs and SJL1p has one NPFXD motif that exactly matches the consensus that we have identified by phage display. Furthermore, NPFXD was recently identified as a new class of endocytosis signal in S.cerevisiae (Tan et al., 1996).
The solution structure of the second domain of Eps15 was recently reported by de Beer et al. (1998). The core of the EH domain (∼75 residues), that shares a significant homology with EF‐hand proteins, folds into two helix‐loop‐helix motifs connected by a short β‐sheet. The C‐terminal 20 residues, which we have shown to contain information necessary for binding to NPF peptides, have a less regular structure. The residues that are involved in the formation of the ‘NPF’ binding pocket were identified by looking at the progressive changes in the 1H, 13C and 15N resonances upon titration with the peptide (PTGSSSTNPFL) corresponding to the C‐terminus of RAB (de Beer et al., 1998). NPF peptides bind to a hydrophobic pocket that is formed by Trp169, Leu155, Leu156 and Leu165 in Eps15‐EH2. In this work we have shown that when either Trp169 or Leu165 are changed into Ala, the ability to bind NPF peptides is lost completely. Consistent with the results of de Beer et al. (1998), more conservative changes at position 169 (W→Y and W→F) have less dramatic effects.
In an attempt to identify residues that modulate peptide recognition, we have tried to relate sequence recognition specificity to EH‐domain primary structure. By aligning and comparing EH‐domain sequences with identical or divergent recognition specificity, we could not identify simple revealing regularities in the primary structures of domains that bind to similar peptides. However, we have spotted a single position (three residues after the conserved tryptophan) whose side chains display some correlation with recognition specificity (Figure 4). We started by comparing the amino acid sequence of the third domain of Eps15 (EH3) with that of Eps15R (EH3R), which although very similar (63% identity), has distinct recognition specificity (Figure 2). EH3 has a negligible affinity for most NPF peptides and preferentially binds to FW‐containing peptides. In contrast, EH3R prefers peptides where the NPF motif is followed by an arginine. The EH3 and EH3R peptide sequences differ in 33 positions. When these variable residues are confronted with the residues that are present in more distant EH domains, a significant correlation between specificity and side‐chain characteristics is found only at position +3 following the conserved tryptophan (position 172 in the Eps15‐EH2 sequence); this is an Ala in EH3R and a Cys in EH3. However, only residues with small side chains are tolerated in the remaining EH domains. Significantly, all the domains that prefer NPF have an Ala or a Ser at this position (Ala being preferred by those requiring an Arg after the NPF motif), while the domains that bind to class II peptides have slightly larger side chains with either Cys or Val. By site‐directed mutagenesis we have shown that it is possible to modulate recognition specificity by directed substitutions at position 172, Ala and Ser favouring binding to NPF peptides, and Cys and Val promoting binding to FW peptides. However, our results suggest that it is not possible to shift recognition specificity from FW to NPF binding and vice versa by only introducing appropriate changes at position 172, and that other changes may be required.
Most EH proteins contain multiple repeats of the EH domain. It is not clear whether, in vivo, each EH domain binds to different protein targets or whether multiple EH domains cooperate in binding to the same protein target, thereby increasing the stability and rigidity of the complex. The relatively low affinity of a single EH‐NPF peptide interaction and the observation that most EH‐protein targets have multiple NPF sequences would favour the second model.
Approximately 50% of the NPF peptides that we have selected have either a Ser or a Thr at position −1 or −2 (Yamabhai et al., 1998; this study). Accordingly, an equivalent (or even larger) enrichment of Ser and Thr is observed in the corresponding positions in the binding peptides that are found in the EH‐domain protein targets (Salcini et al., 1997; Yamabhai et al., 1998). Since we have shown that negative charges at −1 and −2 would negatively affect NPF peptide binding to EH domains, one might speculate that peptide binding could be modulated by a mechanism involving Ser/Thr phosphorylation. Whether this convenient regulatory opportunity has been exploited by natural selection remains to be established.
Materials and methods
Strains and enzymes
Escherichia coli strain DH5α F+ (endA1 hsdR17 (r−k m k+) recA1 endA1 gyrA96 thi‐1 relA1 ▵upE44 f80lacZDM15) was utilized for expression of recombinant proteins and for growth of filamentous phage. Restriction enzymes were purchased from New England Biolabs, Taq polymerase from Perkin Helmer and T4 DNA ligase from Amersham. Oligonucleotides were purchased from Genset and oligopeptides from Genosys.
Expression plasmids were constructed by standard recombinant DNA technology by inserting DNA fragments encoding the relevant EH domain into an expression vector of the pGEX series (Pharmacia). The oligonucleotides utilized to amplify the different EH‐coding sequences and the recipient expression vectors are indicated in Table I. After ligation and electroporation, recombinant clones were identified by PCR, and the inserted DNA fragment was sequenced to exclude the possibility that the PCR procedure had introduced unwanted mutations. EH mutants were obtained by standard site‐directed mutagenesis techniques.
Biopanning (affinity selection)
The construction of the nonapeptide display library and the panning conditions were as previously described (Felici et al., 1991; Dente et al., 1997). The bait protein is prepared as fusion with the GST by affinity purification on a glutathione‐Sepharose resin (Pharmacia).
Every panning cycle was carried out with 4 μg of bait protein and 1010 transducing units (∼2 pmol of random nonamers) in 100 μl phosphate‐buffered saline (PBS), 3% bovine serum albumin (BSA) for 1 h at 4°C. The GST‐EH domain fusion protein was immobilized by binding to 20 μl of gluthatione‐Sepharose matrix. After two or three panning cycles, the selected phage clones were tested in ELISA against their bait proteins. Single‐stranded DNA was prepared as described previously (Dente et al., 1983) and sequenced using an ABI 310 Perkin‐Elmer instrument.
Each well was loaded with 1010 transducing units of the selected phage in 100 μl PBS, 3% BSA. The phage suspension was incubated for 8 h at 4°C, and after washing, 0.2 μg (5 pmol) of the appropriate GST‐fusion protein was applied to each well in 50 ml of PBS, 5% dry milk, and incubated for 2 h at 37°C. After washing, a 1:1000 dilution of an anti‐GST rabbit antiserum (a gift of F.Benfenati) was applied and incubated for 1 h at room temperature. Detection was performed using an alkaline phosphatase conjugated anti‐rabbit goat antibody, diluted 1:1000 (Sigma) and pre‐adsorbed for 2 h with 1011 particles of M13K07.
The chromogenic reaction was developed for 1 h at 37°C by adding 50 μl of 1mg/ml p‐Nitrophenylphosphate disodium, hexahydrate (PNPP; Sigma 104) in 2 mM MgCl2, 50 mM Na2CO3 pH 9.6 to each well. Reading was performed at 405 nm.
We wish to thank Giuliano Nardelli for providing plasmid pYEX and Fabio Benfenati for anti‐GST serum. Michele Quondam constructed the vector expressing one of the deletions in the End3‐EH1 domain. The work done in the laboratory of GC is supported by a grant from the Italian Association for Cancer Research (AIRC) and from MURST. This work was also supported by grants from AIRC to P.P.D.F. and P.G.P., from the Biomed‐2 Program of the European Union to P.P.D.F. and P.G.P., from the Ministero della Sanità (AIDS Program) to P.P.D.F., Armenise‐Harvard Foundation to P.G.P. and P.P.D.F., and from the Ferrero Foundation to P.P.D.F.
- Copyright © 1998 European Molecular Biology Organization