Members of the Plasmodium falciparum var gene family encode clonally variant adhesins, which play an important role in the pathogenicity of tropical malaria. Here we employ a selective panning protocol to generate isogenic P.falciparum populations with defined adhesive phenotypes for CD36, ICAM‐1 and CSA, expressing single and distinct var gene variants. This technique has established the framework for examining var gene expression, its regulation and switching. It was found that var gene switching occurs in situ. Ubiquitous transcription of all var gene variants appears to occur in early ring stages. However, var gene expression is tightly regulated in trophozoites and is exerted through a silencing mechanism. Transcriptional control is mutually exclusive in parasites that express defined adhesive phenotypes. In situ var gene switching is apparently mediated at the level of transcriptional initiation, as demonstrated by nuclear run‐on analyses. Our results suggest that an epigenetic mechanism(s) is involved in var gene regulation.
Controlled phenotypic variation of surface‐exposed antigenic determinants (antigenic variation) is a prime immune evasion mechanism utilized by several pathogenic bacteria and protozoa to maintain a chronic infection in the presence of constant immune pressure exerted by their hosts (Borst and Greaves, 1987; Deitsch et al., 1997). Antigenic variation has been extensively studied in the human pathogens Neisseria gonorrhoeae, Borrelia hermsii and African trypanosomes, where the clonally variant proteins are encoded by members of large gene families. Switching of expression between members of the corresponding gene families occurs through programmed DNA rearrangements (e.g. gene conversion and reciprocal recombination) moving a transcriptionally silent gene into an active expression site, which in the case of Borrelia and African trypanosomes are telomerically located (reviewed in Borst and Rudenko, 1994; Donelson, 1995; Vanhamme and Pays, 1995).
Plasmodium falciparum, the etiological agent of the most virulent malarial species that infects humans, also uses antigenic variation to maintain a persistent infection (Miller et al., 1994 and references therein). Recent studies have demonstrated that the parasite‐encoded erythrocyte membrane protein PfEMP1 is clonally variant. This protein is localized on the surface of the host erythrocyte where it mediates cytoadhesion of the parasitized erythrocytes (P‐RBCs) to either uninfected erythrocytes or the lining of the microvasculature to a wide range of cell surface receptors, including CD36, complement receptor 1 and heparan sulfate, amongst others (Baruch et al., 1995; Rowe et al., 1997; Chen et al., 1998). While sequestration of infected erythrocytes within the microvasculature prevents their splenic clearance, the subsequent occlusion of these capillaries is thought to be a prerequisite for cerebral malaria, a lethal complication of P.falciparum infection (Miller et al., 1994).
PfEMP1 is encoded by a large multigene family, termed var. (Baruch et al., 1995; Smith et al., 1995; Su et al., 1995). The majority of the molecule, encoded by exon I of var genes, seems to be exposed on the erythrocyte surface and has been implicated in host‐cell receptor binding. Exon I codes for 2–5 Duffy‐Binding‐Like (DBL) domains and a cysteine‐rich interdomain region interspersed between DBL1 and DBL2. Apart from short conserved sequence motifs in the first DBL domain (DBL‐1), exon I displays considerable sequence diversity between different var genes (Su et al., 1995). In comparison, exon II, a putative intracellular domain, is relatively well conserved between different var gene variants.
Each parasite contains ∼50–150 var genes per haploid genome, which are distributed throughout all 14 chromosomes, and are located at chromosome‐internal as well as within subtelomeric domains next to the non‐coding repetitive element rep20 (Su et al., 1995; Rubio et al., 1996; Fischer et al., 1997; Hernandez‐Rivas et al., 1997; Thompson et al., 1997). From studies correlating var gene expression with antigenic and adhesive properties of infected erythrocytes it appears that infected erythrocytes express only a limited number of, probably only one, var gene variant at any given time, with var gene switching occuring at a rate of ∼2% per generation in vitro (Roberts et al., 1992; Smith et al., 1995; Rowe et al., 1997; Chen et al., 1998). Although the mechanistic details of var gene expression and switching still remain to be elucidated, initial data have suggested that this mechanism is fundamentally different from that of vsg gene switching in African trypanosomes. var gene expression apparently does not occur from a unique expression site, as expressed var genes were mapped to several different chromosomal loci (Su et al., 1995; Fischer et al., 1997; Hernandez‐Rivas et al., 1997). However, the interpretation of these results has been complicated by the fact that they were derived from long‐term in vitro cultured lines of P.falciparum which were heterogeneous with respect to their antigenic and adhesive properties.
Here we have employed the use of a receptor‐binding panning assay in order to prepare parasite populations with defined adhesive phenotypes, as characterized by their ability to bind specifically to either CD36, intercellular adhesion molecule 1 (ICAM‐1) or chondroitin‐sulfate A (CSA). As each selected parasite population was found to express only a single and distinct var gene variant, this allowed us to investigate the molecular mechanism of var gene expression and switching in an isogenic background. The data presented support the model that var gene expression and switching occur in situ and are controlled at the transcriptional level through an as yet undefined restriction mechanism.
Isolation of parasite populations with defined adhesive phenotypes
In order to investigate the molecular mechanisms responsible for antigenic variation in P.falciparum‐infected erythrocytes, we first prepared parasite populations with defined adhesive phenotypes. Cloned Saimiri brain endothelial cell (SBEC) lines which express either CD36 (SBEC‐C2), ICAM‐1 (SBEC‐3A) or CSA (SBEC‐17) were used as adhesion receptors for P.falciparum‐infected erythrocytes in a panning assay. A long‐term in vitro culture of the P.falciparum clone FCR3, which expresses a heterogeneous set of var gene variants, was selected by four rounds of panning over each of the SBEC cell lines (Figure 1A). The panning selection procedure resulted in an ∼100‐fold enrichment for parasites binding to CSA, 30‐fold for ICAM‐1 and 20‐fold for CD36, compared with the initial unselected parasite culture. The parasite populations obtained are termed here FCR3CD36, FCR3ICAM‐1 and FCR3CSA.
The specificity of the adhesive phenotypes displayed by each of the selected parasite populations was verified as follows: FCR3CD36, FCR3ICAM‐1 and FCR3CSA parasite populations were each panned over Chinese hamster ovary (CHO) cell lines or transfectants thereof expressing high levels of either human CD36 or ICAM‐1 on their surface (Hasler et al., 1993). CSA is naturally expressed on the surface of CHO cells and was removed, where necessary, by incubating CHO cells with chondroitinase ABC prior to the adhesion assay (Rogerson et al., 1995). Each of the parasite populations was found to bind to the CHO cell line expressing the same adhesion receptor as did the corresponding SBEC clone used in the initial panning selection (Figure 1B). In cross‐panning experiments, FCR3CSA parasites did not adhere to CD36‐ or ICAM‐1‐expressing CHO cells. Correspondingly, nor did FCR3CD36 or FCR3ICAM‐1 parasite populations bind to untransfected CHO cells. However, some cross‐adhesive properties, although with reduced binding affinities, were observed for the FCR3CD36 and FCR3ICAM‐1 parasite populations that adhered to ICAM‐1‐ and CD36‐expressing CHO cells, respectively (Figure 1B). This phenomenon has previously been observed and is thought be the result of PfEMP‐1 molecules with more than one binding domain (Baruch et al., 1996; Gardner et al., 1996).
Binding of the selected parasite population was sensitive to the addition of antibodies directed against the corresponding adhesion receptor. For example, binding of FCR3CD36 parasites to SBEC‐C2 expressing CD36 was efficiently inhibited by the CD36‐specific monoclonal antibody FA6‐152 (70% at 5 μg/ml). Similarly, binding of FCR3CSA parasites to SBEC‐17 was abrogated upon the addition of either soluble CSA (>95% at 100 μg/ml) or by pre‐treatment of the CSA‐expressing cell line with chondroitinase ABC (>95% at 1 U/ml) (data not shown). Control experiments were performed to show that anti‐CD36 FA6‐152 antibody had no significant effect on the parasite binding to CSA (12% inhibition at 5 μg/ml). Likewise, soluble CSA or chondroitinase ABC did not significantly hinder parasite binding to CD36 (14% inhibition at 100 μg/ml CSA and 10% inhibition at 1 U/ml chondroitinase). These data demonstrate that parasite populations with defined adhesive phenotypes were obtained.
Each gene variant exhibits a defined adhesive phenotype
In order to determine the genetic basis for these adhesive phenotypes, total RNA from each selected parasite population was isolated (immediately after panning over their respective SBEC cell line) and subjected to reverse‐transcription–PCR (RT–PCR) amplification using the oligonucleotides varA5.2 and varB3.2 as primers. These oligonucleotides are degenerate and recognize conserved motifs within the DBL‐1 domains of var genes. Variability within the DBL1 domains results in PCR products that are specific for the respective var gene variant(s) expressed (Smith et al., 1995; Su et al., 1995; Fischer et al., 1997; Hernandez‐Rivas et al., 1997). The resulting PCR products, ∼500 bp in size, were cloned and 10 clones from each parasite population sequenced. In the case of both FCR3CSA and FCR3ICAM‐1, each of the 10 clones analysed contained an identical PCR product. A sequence analysis confirmed that the PCR products obtained were homologous to DBL‐1 domains of var gene variants, which henceforth are called varCSA and varICAM‐1. The experiment was independently repeated for the FCR3CSA parasite population, and a set of 40 PCR products analysed. All 40 PCR products were found to be identical, corresponding to the var gene variant varCSA identified in the previous experiment. For FCR3CD36, nine of the 10 PCR products analysed were identical and homologous to a DBL1 domain of a var gene variant henceforth termed varCD36. A comparative sequence analysis of the deduced amino acid sequences of the DBL1 domains of varCSA, varCD36 and varICAM‐1 revealed that they are distinct from one another, and also from the var gene variant varT3‐1 predominantly expressed in the parental FCR3 population (Hernandez‐Rivas et al., 1997; see Figure 2). These data indicate that each parasite population expresses a single and distinct var gene variant, which apparently mediates the defined adhesive phenotype responsible for binding to either CSA, ICAM‐1 or CD36.
To confirm the conclusion that each selected parasite population expresses a single and distinct var gene variant, cross‐hybridization experiments were performed. Sequence tags specific for the DBL1 domains of the var gene variants varCSA, varCD36 and varICAM‐1 were each hybridized against filters containing either size‐fractionated total RNA isolated from FCR3, FCR3CSA, FCR3CD36 and FCR3ICAM‐1, or the RT–PCR products generated from each of them using the degenerated primers to the DBL‐1 domain of var genes. It was found that each var gene tag hybridized only to the RNA or RT–PCR products of the parasite population from which it was derived, and no other (Figure 3A and B). The Northern analysis revealed that the varCD36 and varICAM‐1 transcripts are between 7 and 9 kb in size (Su et al., 1995), typical for var genes, whereas the varCSA transcript has an unprecedented small size of ∼4.4 kb, suggesting a var gene structure composed solely of one or perhaps two DBL domains. These data support our hypothesis that upon selection for defined adhesive properties, parasite populations are obtained that express a unique and distinct var gene variant.
In situ activation of var gene expression
Previous data, derived from parasite populations with heterogeneous antigenic and adhesive phenotypes, have suggested that var gene expression, unlike vsg gene expression in African trypanosomes, is not linked to a conserved expression site, but rather occurs in situ, irrespective of the chromosomal location (Fischer et al., 1997). To verify this hypothesis, we have mapped the chromosomal location of the var gene variants identified within the selected parasite populations in which the var gene variants varCSA, varCD36 and varICAM‐1 are either transcriptionally active or silent. Agarose blocks containing intact chromosomes from FCR3, FCR3CD36, FCR3ICAM‐1 and FCR3CSA were prepared, size‐fractionated and examined by Southern analysis using specific probes to varT3‐1, varCSA, varCD36 and varICAM‐1. The chromosomal location of each var gene variant investigated (mapping to chromosomes 3, 10, 13 and 12, respectively) remains unchanged irrespective of its transcriptional status (Figure 4A and B). Fine‐scale restriction mapping, using the endonucleases ApaI, BssHII, SmaI, RsaI, DraI and Sau3A, confirmed this finding (data not shown). varCSA and varCD36 consistently map to subtelomeric domains of the chromosomes 10 and 13, respectively, in close proximity to the rep20 sequence element, whereas varICAM‐1 always lies within a central domain of chromosome 12 (Figure 4C). Furthermore, no significant DNA rearrangements were observed in the immediate vicinity of the var gene variants investigated upon transcriptional activation or silencing. Thus, switching between var gene variants appears to occur in situ, independent of the chromosomal location, and apparently does not require the transposition of a var gene to a specific, possibly telomerically located, expression site.
The modification of nucleotides can play a significant role in the control of gene expression, as exemplified by 5′methylcytosines in higher eukaryotes and β‐d‐glucosyl‐hydroxymethyluracil, base J, in African trypanosomes (Ceder, 1988; Gommers‐Ampt et al., 1993). Given that there is evidence for DNA methylation in P.falciparum (Pollack et al., 1991), we explored the possibility that CpG methylation might play a role in differential var gene expression. DNA prepared from trophozoites of FCR3, FCR3CSA, FCR3CD36 and FRC3ICAM‐1 was restricted using the methylation‐sensitive endonucleases HpaII, HhaI, RsaI and Sau3A, size‐fractionated and hybridized with probes specific for the var gene variants varT3‐1, varCSA, varCD36 and varICAM‐1 (data not shown). The hybridization patterns observed are identical, irrespective of the transcriptional status of the var gene variant in each selected parasite population, which would suggest that changes in the methylation pattern play no role in var gene switching.
Allelic exclusion of var gene expression is transcriptionally regulated
That var genes are developmentally expressed has been inferred from studies using heterogeneous parasite populations, in which var gene transcripts have been detected only during the early stages of intra‐erythrocytic parasite development (Fischer et al., 1997). To define better the onset of var gene expression, a FCR3CSA parasite population was selected for CSA binding and the resulting parasites (early schizonts) allowed to reinvade. Total RNA was then isolated 10 h (rings) and 24 h (trophozoites) post‐invasion, and the var gene expression pattern analysed by RT–PCR using the degenerate oligonucleotide primers to the DBL‐1 domain of var genes (Figure 5A). A unique PCR product was obtained from the trophozoite stage RNA, which corresponds to varCSA, as demonstrated by analysis of 40 independent clones (Figure 5A and B). In comparison, an ill‐defined PCR product was obtained from the ring‐stage RNA, which, surprisingly, corresponds to a large set of at least 14 different var gene variants, as shown by the sequencing of 30 independent clones (Figure 5B). No PCR products were obtained in the absence of reverse transcriptase, indicating that genomic DNA did not contaminate the RNA preparations. The apparent ubiquitous transcription of var gene variants in rings, as compared with trophozoites, was confirmed by hybridization analyses in which the respective RT–PCR products were hybridized against filters containing specific sequence tags for 19 different var gene variants present in the FCR3 genome (Figure 5C). Sequence tags for var gene variants were generated by PCR amplification of genomic DNA using the degenerate oligonucleotides varA5.2 and varB3.2 as primers. Whereas the probe derived from trophozoite RNA identified the expected single fragment corresponding to varCSA, the probe derived from ring RNA hybridized to all sequence tags under stringent hybridization conditions, indicative of the expression of several members of the var gene family in ring‐stage parasites. Together, these findings suggest that allelic exclusion of var gene expression is regulated during parasite development, i.e. transcription of most, if not all, var gene variants in early ring stages is restricted to a single var gene variant as the parasite matures into a trophozoite.
The distinct expression of a var gene variant in a subsequent non‐selected infective cycle would suggest some form of imprinting. To exclude the possibility that our panning procedure causes an aberrant arrest in var gene switching, thereby fixing the var gene variant expressed, we tested the ability of a selected parasite population to switch to other var gene variants. A freshly selected FCR3CD36 parasite population was panned over CHO cells expressing CSA, and the var gene variant expressed in the resulting parasite population determined. It was found that the resulting parasite population adheres to CSA and expresses the var gene variant varCSA. This finding indicates that the original FCR3CD36 parasite population had maintained its ability to switch var gene expression.
Expression of var genes during parasite maturation, presumably resulting from some form of restriction in the expression of other members of the var gene repertoire, could be regulated either at the transcriptional or the post‐transcriptional level. To address this question, a nuclear run‐on analysis was carried out using nuclei prepared from trophozoites of both FCR3CSA and FCR3CD36 parasite populations. Radiolabelled nascent RNA, isolated from ∼3×109 infected erythrocytes, was hybridized to a filter containing DNA specific for the five var gene variants varT11‐1, varT3‐1, varCSA, varCD36 and varICAM‐1, the gene hrp2, an rRNA subunit gene and the unrelated pUC18 plasmid. Whereas nascent RNA isolated from both FCR3CSA and FCR3CD36 trophozoites hybridizes to both hrp2 and rRNA, but not pUC18, the RNA derived from FCR3CSA hybridizes exclusively to the varCSA gene variant, and vice versa the FCR3CD36 RNA to varCD36 (Figure 6). This finding clearly demonstrates that var gene expression is regulated at the level of transcription.
Our data set the framework for a better understanding of the mechanism(s) underpinning antigenic variation in P.falciparum. Var genes play an important role in the pathogenicity of P.falciparum, encoding immunovariant adhesins (PfEMP1) that mediate binding of the host erythrocyte to the endothelial lining of the microvasculature and uninfected erythrocytes. A wide range of different host cell surface molecules, including CSA, ICAM‐1 and CD36, serve as receptors for PfEMP1 binding and differences in parasite adhesion properties appear to influence disease outcome (Carlson et al., 1990; Turner et al., 1994; Fried and Duffy, 1996). Studies correlating var gene expression patterns with serological and adhesive phenotypes of the infected erythrocyte have suggested that var genes are differentially expressed in P.falciparum, i.e. a single cell expresses only a limited number of, possibly only one, var gene variant from its repertoire at any given time (Rowe et al., 1997; Chen et al., 1998). Verification of this model, however, has been difficult to obtain due to the heterogeneous nature of parasite populations cultured in vitro, which, in the absence of a selective pressure, express many different var gene variants at any given time. By employing a receptor panning assay, based on CHO or SBEC cells expressing different receptors for PfEMP1 binding on their surface, we were able to prepare large parasite populations with homogeneous and defined adhesive phenotypes. Genetic analysis of the var gene repertoire expressed in each selected parasite population shows that only a single var gene variant is expressed, with the exception of those parasites selected for CD36 binding where a second expressed var gene variant was detected, albeit at a very low level. No other expressed var gene variants were detected in the selected parasite populations inspite of an intensive search employing both RT–PCR and Northern analyses. This finding clearly demonstrates that each individual parasite encodes only a single var gene variant.
A comparative sequence analysis revealed that the var gene variants expressed in the different FCR3 parasite populations are distinct from one another, an observation confirmed by their different chromosomal locations. Interestingly, independent panning experiments consistently identified that the same var gene variant is responsible for a particular adhesive phenotype, irrespective of the adhesive properties of the starting parasite population. For example, the FCR3CD36 parasite population selected for CSA binding would always switch back to the same varCSA upon selection on CHO cells (data not shown). This finding poses the question of whether each var gene variant mediates a defined adhesive phenotype. Although only 13 targets for PfEMP1 binding have thus far been identified (namely, the endothelial receptors thrombospondin (Roberts et al., 1985), CD36 (Ockenhouse et al., 1989), ICAM‐1 (Berendt et al., 1989), E‐selectin (Ockenhouse et al., 1992), VCAM‐1 (Ockenhouse et al., 1992), CSA (Robert et al., 1995; Rogerson et al., 1995), PECAM‐1 (Treutiger et al., 1997), the erythrocyte receptors heparan sulfate (Carlson et al., 1992; Chen et al., 1998), bloodgroup sugars A and B (Carlson and Wahlgren, 1992), complement receptor 1 (Rowe et al., 1997), and the serum proteins IgG/IgM and fibrinogen (Scholander et al., 1996), this number is steadily increasing as we investigate this pathogenic adhesive phenomenon in more detail. The parasite clone FCR3 is able to bind to most if not all of these host molecules, and, significantly, expresses a different var gene variant to mediate each adhesive phenotype upon selection (Treutiger et al., 1997; Chen et al., 1998; Fernandez et al., 1998; this study).
Although it is possible that each var gene variants mediates a defined adhesive phenotype, other interpretations of the data need to be considered. The panning procedure employed in this study may consistently select for that var gene variant with the highest affinity for the corresponding receptor. Accordingly, there may be other var gene variants in the FCR3 parasite genome that mediate binding to CD36, ICAM‐1 or CSA, although with a lower affinity. The ability to bind CD36 has been suggested to be an intrinsic adhesive property of infected erythrocytes, as inferred from binding studies using clinical isolates (Ockenhouse et al., 1991; Newbold et al., 1997). This property has been attributed to a conserved binding domain of PfEMP1, such as the CIDR domain (Baruch et al., 1995, 1997). That parasite populations selected for CSA binding did not bind CD36, unlike the FCR3ICAM‐1 parasite subpopulation, may be attributed to the unusual composition of the PfEMP1 variant encoded by varCSA. Preliminary results show that it contains only two DBL domains and a region with little homology to the CIDR domain implicated in CD36 binding (unpublished data).
The ability of infected erythrocytes to bind to CSA appears to be an important pathogenic factor in maternal malaria, as previously suggested (Fried and Duffy, 1996). Interestingly, CSA binding is not a general property of all P.falciparum strains. A screening of several geographically distinct P.falciparum isolates revealed that only some are capable of binding CSA, despite several rounds of selection (data not shown). Moreover, non‐CSA binding parasites do not hybridize with the varCSA specific probe, whereas parasites capable of binding CSA do, as demonstrated by Southern analysis (data not shown). This finding supports our model that individual var genes mediate defined adhesive phenotypes, which, when displayed, may affect the outcome of the disease.
Since all of the three parasite populations are derived from the same parental clone and are interconvertable in terms of their var gene variant expressed upon appropriate selection, the opportunity arises of investigating var gene switching within isogenic parasites. Mapping the location of expressed and silent var gene variants within these isogenic parasite populations clearly indicates that var gene expression is not linked to a specific expression site, but rather occurs in situ, irrespective of a particular chromosomal location. While the varCSA and varCD36 gene variants are expressed from subtelomeric locations within chromosomes 10 and 3, respectively, varICAM‐1 is expressed from a central location within chromosome 12. In situ activation of var genes is supported by previous data which show expression of var genes from both subtelomeric and chromosome internal domains of numerous chromosomes (Su et al., 1995; Fischer et al., 1997; Hernandez‐Rivas et al., 1997). Thus, the mechanism of var gene switching in P.falciparum is fundamentally different from switching immunovariant genes in other pathogens (Borst and Greaves, 1987; Deitsch et al., 1997). For example, in African trypanosomes, the active vsg gene is transcribed from a specific telomeric expression site, with antigenic variation being habitually mediated through replacing the active vsg gene variant with one of the 1000 silent copies present elsewhere in the genome. However, in this parasite antigenic variation can also occur by switching expression between different vsg genes, suggesting that some form of allelic exclusion is operating in African trypanosomes as well.
While var gene expression is tightly regulated in trophozoites, ubiquitous transcription of all var gene variants appears to occur in early ring stages, with no predominance of any one var gene variant. It has been speculated that the var genes could be controlled at the level of RNA processing by partial degradation of all mRNAs but one (Borst et al., 1995). Although differential RNA degradation would be a plausible interpretation of these data, additional experimental evidence points to a different switching mechanism. First, relaxed gene transcription in ring stages is not limited solely to var genes, rather there appears to be a general pattern of relaxed transcription of many, if not all, P.falciparum genes at this point in the parasite's life‐cycle. Specific RT–PCR reactions identified transcription of both gametocyte and sporozoite specific genes in early rings, including the Pf11‐1 and circumsporozoite (CS) genes, although at a very low level (data not shown). Secondly, the phenomenon of in situ var gene switching is apparently mediated at the level of transcriptional initiation, as demonstrated by nuclear run‐on analyses. Radiolabelled nascent RNA isolated from trophozoite parasite populations selected for CSA binding hybridized only to a probe corresponding to the varCSA gene variant. Similarly, radiolabelled nascent RNA isolated from trophozoites selected over CD36 identified only the varCD36 gene variant, and no other. Taken together, these data indicate that each var gene is an independent transcription unit in which promoter activity determines the expression status. Activation and silencing of var gene promoter activity seems not to involve DNA rearrangements, nor changes in the DNA methylation pattern, as demonstrated by extensive restriction analyses.
Given that maturation of the parasite results in the mutually exclusive expression of a single var gene variant, this would indicate that some mechanism is responsible for silencing the activity of all but one var gene promoter. It is tempting to speculate that the control of var gene switching is mediated through this silencing mechanism, focusing towards a var gene variant different from that expressed in the previous cycle, albeit at a low rate. How this effect is brought about remains to be determined. Drawing together our results, we hypothesize that an epigenetic mechanism(s) is involved in var gene regulation. It has been demonstrated that transcriptional silencing can be overcome by changes in chromatin states through reversible histone acetylation (Wolffe, 1996). Recently, histone acetylation/deacetylation was described in P.falciparum blood stage parasites (Darkin‐Rattray et al., 1996). Thus, chromatin structure analysis might give us a deeper understanding of the factors that regulate the in situ activation of the multigene var family.
Materials and methods
Parasites and cell lines
P.falciparum FCR3 parasites were cultivated according to Trager and Jensen (1976). Stable transformants of CHO cells expressing CD36 or ICAM‐1 have been described elsewhere (Hasler et al., 1993). Recently, several clones of Saimiri brain microvascular endothelial cells (SBEC) were isolated that express either CD36 (SBEC‐C2), ICAM‐1 (SBEC‐3A) or CSA (SBEC‐17) as parasite adhesion receptor (Gay et al., 1995; Robert et al., 1995). Cell culture and P‐RBC cytoadherence assays of SBEC were performed as described previously (Gay et al., 1995; Robert et al., 1995). CSA inhibition assays and the removal of chondroitin‐sulfate A from SBEC or CHO cells was performed as described (Robert et al., 1995; Rogerson et al., 1995). Inhibition studies of cytoadherence of P‐RBCs using anti‐CD36 mAb FA6‐152 (obtained from Dr L.Edelman, Institut Pasteur, Paris, France) and anti‐ICAM‐1 mAb 84H10 (Immunotech) were carried out as described previously (Gysin et al., 1997; Pouvelle et al., 1997).
Panning of parasites on endothelial cells
The initial FCR3 parasites showed low binding or no detectable binding to the SBEC cells used in this study. The panning assay was performed essentially as described previously (Pouvelle et al., 1997). For the isolation of large amounts of adherent parasites, 150 cm2 culture flasks containing semiconfluent SBEC were used. Bound parasites were detached using an automatic pipette aid and a 5 ml pipette. Free P‐RBCs were centrifuged and used to prepare DNA and total RNA. In some instances, the parasites were allowed to reinvade before the DNA and RNA isolation.
Nuclear run‐on analysis
Nuclear run‐on analysis on P.falciparum asexual bloodstage parasites was performed as described previously (Lanzer et al., 1994); 0.5 pmol of P.falciparum gene fragments cloned into pUC18 were denatured and transferred onto a nylon membrane (Hybond N+, Amersham) using a dot blot apparatus as described previously (Ausubel et al., 1998). The following P.falciparum genes were used: rRNA (Langsley et al., 1983), HRPII (Wellems and Howard, 1986), FCR3 DBL‐1 PCR fragments of varT3‐1 and varT11‐1 (Hernandez‐Rivas et al., 1997). varCSA, varCD36 and varICAM‐1 are described in this work.
Pulsed‐field gel‐electrophoresis (PFGE) and two‐dimensional (2‐D) PFGE of P.falciparum chromosomes was performed in contour‐clamped homogeneous electric field apparatus as described (Hernandez‐Rivas and Scherf, 1997).
RT–PCR and Northern analysis
Total RNA was prepared using the Qiagen RNA isolation kit according to the recommendations of the manufacturer. The RNA was treated with DNase free of RNase (Pharmacia) and repurifed on the Qiagen RNA columns. Ten micrograms of total RNA was fractionated on a 1% agarose‐formaldehyde gel, transferred to a nylon membrane (Hybond N+) and hybridized with random‐primer‐labelled probes (Hernandez‐Rivas et al., 1997). For first‐strand cDNA synthesis, 1 μg of total RNA treated with RNase‐free DNase was obtained by annealing RNA with oligo (dT)‐primers and extending the strand with reverse transcriptase as recommended by the manufacturer (Perkin–Elmer–Cetus). varA5.2 and varE3.2 were used to amplify DBL domain 1 (Hernandez‐Rivas et al., 1997). RT–PCR without the addition of the reverse transcriptase and RNA treated with RNase were used as controls. RT–PCR products were extracted from agarose gels using the Qiagen PCR purification kit.
Southern blot and hybridization
Digested DNA was separated on agarose gels and transferred to Hybond N+ (Amersham) membrane as recommended by the manufacturer. DNA probes were hybridized in 7% SDS, 1% bovine serum albumin (BSA) and 0.5 M NaPO4 pH 7.4, and washed under stringent wash conditions: 0.1× SSC, 0.1% SDS at 60°C. Dot blot hybridization of various var DBL‐1 DNA inserts was performed as described (Ausubel et al., 1998) using a dot blot apparatus (Schleicher and Schüll) and 20 ng of DNA per dot on a Hybond N+ (Amersham) membrane.
We thank Paul Horrocks and Charles Roth for critically reading the manuscript and invaluable discussions. This work has been supported by a grant from the Commission of the European Communities for research and technical development (Contract No. CT96‐0071), GDR and French Army grant (Contract No. DSP/STTC‐ 97/070). P.B. was supported by Fondation Mérieux and Fond d'Etudes de l'Assistance Publique/Hopitaux de Paris.
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