We have exploited the recently developed ability to trans‐ fect the malaria parasite Plasmodium falciparum to investigate the role of polymorphisms in the enzyme dihydropteroate synthase (DHPS), identified in sulfadoxine‐resistant field isolates. By using a truncated form of the dhps gene, specific mutations were introduced into the endogenous gene by allelic replacement such that they were under the control of the endogenous promoter. Using this approach a series of mutant dhps alleles that mirror P.falciparum variants found in field isolates were found to confer different levels of sulfadoxine resistance. This analysis shows that alteration of Ala437 to Gly (A437G) confers on the parasite a 5‐fold increase in sulfadoxine resistance and addition of further mutations increases the level of resistance to 24‐fold above that seen for the transfectant expressing the wild‐type dhps allele. This indicates that resistance to high levels of sulfadoxine in P.falciparum has arisen by an accumulation of mutations and that Gly437 is a key residue, consistent with its occurrence in most dhps alleles from resistant isolates. These studies provide proof that the mechanism of resistance to sulfadoxine in P.falciparum involves mutations in the dhps gene and determines the relative contribution of these mutations to this phenotype.
Malaria parasites are the cause of approximately two million deaths annually, and the most severe form of the disease arises from infection with Plasmodium falciparum. The sulfone/sulfonamide (sulfa) group of compounds are potent inhibitors of P.falciparum and are used for the control of malaria. However, the widespread appearance of resistant parasites, particularly in southeast Asia, has reduced their utility (Landgraf et al., 1994). Importantly, this group of drugs is still effective in Africa where resistance is less prevalent. Sulfadoxine is the most commonly used sulfa drug and is usually used in a combination with pyrimethamine known as Fansidar, as the two drugs show a marked synergism in their antimalarial action (Chulay et al., 1984).
Sulfadoxine acts as a competitive inhibitor of the enzyme dihydropteroate synthase (DHPS) (Triglia et al., 1997) which is in the folate biosynthetic pathway and couples p‐aminobenzoic acid (pABA) with 6‐hydroxymethyldihydropterin pyrophosphate to yield 7,8‐dihydropteroate (Brown, 1971). In P.falciparum, DHPS is a bifunctional enzyme (PPPK‐DHPS) with 7,8‐dihydro‐6‐hydroxymethylpterin pyrophosphokinase (PPPK), the enzyme preceding DHPS in the folate biosynthetic pathway (Brooks et al., 1994; Triglia and Cowman, 1994). The P.falciparum dhps gene from sulfadoxine‐resistant and ‐sensitive parasites has been cloned and differences between the amino acid sequences encoded by them have been identified, suggesting that they may be involved in the mechanism of resistance to this drug (Brooks et al., 1994; Triglia and Cowman, 1994; Wang et al., 1995, 1997a; Triglia et al., 1997).
Expression of variant forms of P.falciparum PPPK‐DHPS in Escherichia coli and subsequent kinetic analysis of the purified enzymes has shown that the mutations decrease the affinity of binding to sulfadoxine, which is consistent with their importance in the mechanism of resistance to this drug (Triglia et al., 1997). Additional evidence for the role of DHPS in sulfadoxine resistance has been obtained by analysis of a genetic cross between sulfadoxine‐resistant and ‐sensitive P.falciparum parents in which the mutations in the dhps gene were shown to segregate with the drug‐resistant phenotype (Wang et al., 1997b). Results from this cross have also suggested that an additional gene that affects folate utilization can affect the level of sulfadoxine resistance.
The recent development of transfection in different Plasmodium species, including P.falciparum (van Dijk et al., 1995; Wu et al., 1995, 1996; Crabb and Cowman, 1996), has now made it possible to determine directly the role of allelic variants in phenotypes such as sulfadoxine resistance. Integration of plasmid constructs into the P.falciparum genome appears always to occur by single recombination events as no double recombination events have yet been described (Wu et al., 1996; Crabb et al., 1997a,b).
The inability to select double recombination events in this parasite makes it difficult to engineer gene replacements to determine the specific contribution of mutations in a gene to phenotypes such as drug resistance. In this study we have therefore made constructs containing truncated mutant dhps alleles which, when integrated into the P.falciparum genome, result in allelic exchange and expression of a functional mutant dhps gene under the control of its endogenous promoter. Exploiting a recently developed, highly sensitive assay that allows good resolution of sulfadoxine susceptibilities (Wang et al., 1997c), we show that six different pppk‐dhps mutant alleles transfected into sulfadoxine‐sensitive parasites confer different levels of sulfadoxine resistance. This demonstrates that mutations in the dhps gene are responsible for sulfadoxine resistance in P.falciparum.
Transfection of a full‐length pppk‐dhps gene results in pseudo‐polyploidy
In order to test if mutations identified in the dhps gene of P.falciparum are responsible for sulfadoxine resistance we initially made two constructs in the transfection plasmid pHC1 (Crabb et al., 1997b), both of which contain the full‐length pppk‐dhps gene (Brooks et al., 1994; Triglia and Cowman, 1994). The first encoded the wild‐type pppk‐dhps gene as a control, while the second contained the pppk‐dhps allele from W2mef, a highly sulfadoxine‐resistant isolate possessing three amino acid differences in the dhps domain compared with the wild‐type gene (Triglia et al., 1997). The constructs were transfected into the sulfadoxine‐sensitive cloned line D10 and transfectants selected for integration. Southern hybridization analysis of different cloned transfectants showed that the plasmid had integrated between one and four copies by single recombination events producing parasites with multiple copies of the pppk‐dhps gene under control of either the endogenous promoter or the introduced calmodulin promoter (data not shown). Analysis of the transfectants showed that those carrying the W2mef allele were resistant to considerably higher levels of sulfadoxine than those in which the wild‐type gene had been introduced. However, the presence of different numbers of integrated and expressed copies of the pppk‐dhps gene made it difficult to quantitate the contribution of the mutations to the drug resistance phenotype and to extend this type of analysis to the role of individual mutations.
Homologous recombination of a truncated pppk‐dhps gene leads to allelic exchange
We thus sought to ensure that integration of the pppk‐dhps transfection plasmids by a single recombination event would result in just a single active gene that would be driven from the endogenous promoter. To this end, we used a pppk‐dhps gene from which the 5′‐most 100 bp had been removed, corresponding to the codons for the initiation methionine and the subsequent 33 amino acids (Figure 1). Integration of this construct into the endogenous pppk‐dhps gene by homologous recombination should insert the mutations in dhps that are 3′ to the recombination event by allelic exchange. Importantly, multiple plasmid insertion events would not create additional full‐length pppk‐dhps genes as they would be truncated and unlikely to express functional enzyme.
Using this approach, truncated pppk‐dhps genes from a sulfadoxine‐sensitive isolate and five different resistant forms were inserted into the plasmid vector pHC1 (Crabb et al., 1997b) such that they would integrate into the endogenous pppk‐dhps gene as described above (Figure 1). Transfectants were selected on pyrimethamine and grown for 3‐week periods without drug pressure to select for parasites that contain integrated copies of each plasmid. All transfectants were cloned and one clone of each, called D10‐sakaa, D10‐sgkaa, D10‐sgkga, D10‐agkaa, D10‐ageaa and D10‐fgkas (Table I), was analysed further.
Integration of truncated dhps plasmids occurs at the pppk‐dhps gene on chromosome 8
To determine if integration of the transfection plasmids had occurred, chromosomes from each clone were separated by pulsed‐field gel electrophoresis (PFGE) (Chu et al., 1986), transferred to nylon filters and hybridized with both the dhps and Toxoplasma gondii dhfr genes (Figure 2). The ethidium bromide stained chromosome pattern was identical for D10 and the six transfected clones, indicating that all were derived from the parental, pyrimethamine‐sensitive parasite line. As expected, the P.falciparum dhps probe hybridized to chromosome 8 in the parental D10 line (Brooks et al., 1994; Triglia and Cowman, 1994) and in the transfected clones. The T.gondii dhfr probe did not hybridize to D10 but gave an otherwise identical hybridization pattern showing, in the six different transfected P.falciparum lines, that integration had occurred on chromosome 8 in each case.
To prove that integration of the plasmid on chromosome 8 had occurred at the dhps locus, Southern blot analysis was used to probe genomic DNA of both untransfected D10 and the six transfectants. The dhps probe was hybridized to EcoRI–ClaI‐digested DNA (Figure 3A). In D10 a band of 3.6 kb was observed, whereas the transfected clones gave bands of 2.9, 4.2 and 4.8 kb, indicating that the transfected plasmid construct had indeed integrated into the pppk‐dhps gene. It was evident from band intensities in the D10‐sgkaa and D10‐fgkas samples that the 4.2 kb EcoRI–ClaI fragment was present at a higher copy number with respect to the other transfected clones. The copy number of this band in the six transfectants was determined using phosphorimage analysis and found to be in a 3:1 ratio with respect to the 2.9 kb band in both D10‐sgkaa and D10‐fgkas, but in a 1:1 ratio in the other clones. This indicated that two copies of the transfection plasmid had integrated into D10‐sakaa, D10‐agkaa, D10‐sgkga and D10‐ageaa, whereas D10‐sgkaa and D10‐fgkas had each acquired four copies (Figure 3C).
Homologous recombination events occurred 5′ to the dhps mutations
Although integration of the plasmid constructs had occurred in the endogenous pppk‐dhps gene, it was possible that this had taken place 3′ to the mutations in dhps, which would result in the absence of these mutations in the expressed allele. It was thus important to verify that the recombination event had occurred 5′ of the mutations in dhps to give allelic exchange. The presence of an AvaII site, associated with the Ala–Gly substitution at amino acid 437, was used to determine if the D10‐sgkaa, D10‐sgkga, D10‐agkaa, D10‐ageaa and D10‐fgkas cloned lines had undergone integration events 5′ of this position. AvaII‐digested genomic DNA of both untransfected D10 and the six transfected clones was hybridized with the dhps probe (Figure 3B). If recombination had occurred 5′ to the AvaII site in the mutant dhps genes, a hybridizing DNA band of 3.1 kb would be expected, and this was observed for D10‐sgkaa, D10‐sgkga, D10‐agkaa, D10‐ageaa and D10‐fgkas. As the pppk‐dhps gene transfected into D10‐sakaa did not have the Gly437 codon mutation there was no AvaII site and bands of 8.0, 6.7 and 16 kb were obtained. These results confirmed that in all cases the recombination events had occurred 5′ of the 437 codon and that the endogenous allele had been replaced by the mutant dhps alleles.
The integrated dhps alleles are transcribed
To determine whether the integrated dhps alleles were transcribed, DNase‐treated poly(A)+ RNA was made from each clone and cDNA synthesized for reverse transcriptase–polymerase chain reaction (RT–PCR), prior to amplification with dhps (primer 1; Figure 1) and hsp86 3′ (primer 2; Figure 1) oligonucleotides. The expected product of 1323 bp was obtained for the six transfectants. When the PCR products were digested with AvaII, D10‐sgkaa, D10‐sgkga, D10‐agkaa, D10‐ageaa and D10‐fgkas gave fragments of 959 and 364 bp, indicating that a dhps gene encoding the Gly437 mutation is transcribed in these transfected lines (Figure 4). The D10‐sakaa RT–PCR product was resistant to digestion with AvaII as expected, as the pppk‐dhps allele transfected was the wild‐type. Similar RT–PCR experiments were performed as controls using two oligonucleotides that were both from the dhps gene, to test if the disrupted endogenous dhps gene was still transcribed. Residual transcription from this gene was at an almost undetectable level (<1% of total) in all of the P.falciparum lines transfected with a dhps gene encoding Gly437 (data not shown). In addition, this gene is truncated and unlikely to express a functional enzyme. Sequencing of RT–PCR products amplified with oligonucleotides #1 and #2 (Figure 1) confirmed that the genotype of the exchanged dhps allele in each clone was as expected for each transfectant (Table I). The cDNA synthesized from RNA isolated from each of the different lines was tested for the absence of DNA contamination by using oligonucleotides across the intron of the pabAB gene (T.Triglia and A.F.Cowman, in preparation). None of the RNA samples used for further analysis showed any such contamination. Therefore allelic exchange has replaced the endogenous pppk‐dhps and the new alleles with the desired mutations are all transcribed.
The new pppk‐dhps alleles are expressed
To determine whether the integrated pppk‐dhps alleles in the different transfectants were expressed, proteins from synchronized trophozoites of each parasite line were separated by SDS–PAGE and transferred to nitrocellulose. Antibodies to dhps detected a protein band of 83 kDa in the D10 parent and the six transfectants (Figure 5), a size which corresponds to the predicted molecular weight of the 706 amino acid bifunctional enzyme (Brooks et al., 1994; Triglia and Cowman, 1994). There was no evidence for translation of truncated proteins that may have initiated from internal methionine codons, although we did observe consistent reactivity with an unidentified 92 kDa band in all the transfected clones. Probing of an identical filter using anti‐hsp70 antibodies (Bianco et al., 1986) showed that there was an approximately equal amount of protein loaded in each lane, suggesting that the level of expression of the full‐length PPPK‐DHPS enzyme in the transfected parasite lines is similar to that in the untransfected D10 parent.
The mutant dhps alleles confer sulfadoxine resistance
To determine whether the different mutant alleles of pppk‐dhps render D10 parasites resistant to sulfadoxine, parasites were firstly adapted to growth in a low folate/pABA medium to permit accurate measurement of the inhibitory concentration of sulfadoxine that kills 50% of the parasites (IC50) (Wang et al., 1997b,c). The D10 parental line had an IC50 of 32 ± 8 ng/ml whereas the D10‐sakaa transgenic (transfected with the wild‐type allele of pppk‐dhps) was consistently more resistant to the drug (129 ± 63 ng/ml) (Table I). Both of these parasite lines express the wild‐type form of the PPPK‐DHPS enzyme and the increase in the level of resistance may reflect a small increase in the level of expression of the protein, or the addition of the modified T.gondii dhfr gene resistant to pyrimethamine may somehow affect the level of sulfadoxine resistance.
Importantly, a single mutation in the dhps gene converting Ala437 to Gly437 (A437G) increased the level of resistance of the parasite 5‐fold to 614 ± 229 ng/ml compared with that seen for D10‐sakaa (Table I). Addition of the further mutation A581G in D10‐sgkga or S436A in D10‐agkaa further increased the level of resistance to the drug, but by a smaller margin, suggesting that these amino acid changes have a lesser effect on the ability of sulfadoxine to inhibit the enzyme. The mutation at residue 540 (K540E) increased the level of resistance ∼2‐fold for the transfectant D10‐ageaa compared with D10‐sgkaa but was 10‐fold more resistant to the drug compared with the D10 allele (D10‐sakaa). The highest level of resistance was obtained with the W2mef allele of pppk‐dhps (D10‐fgkas), which originates from a highly sulfadoxine‐resistant line of P.falciparum. The insertion of the three amino acids corresponding to this allele yielded a sulfadoxine‐resistance phenotype that was 24‐fold higher compared with that obtained with the wild‐type allele (D10‐sakaa). The pattern of increasing resistance to sulfadoxine as the number of mutations rises suggests that resistance to this drug arose by an accumulation of mutations due to increasing levels of drug pressure. Importantly, these results prove conclusively that mutations in the dhps gene of P.falciparum confer resistance to this drug on the parasite.
The recently developed ability to transfect P.falciparum (Crabb and Cowman, 1996; Wu et al., 1996) provides a powerful means to investigate the role of dhps mutations in the mechanism of resistance to sulfadoxine. We have devised a novel allelic replacement strategy to introduce mutant pppk‐dhps genes at the endogenous locus, retaining transcriptional control from the autologous promoter. Although the 3′ non‐coding region of the pppk‐dhps gene is replaced with the 3′ end of hsp86 in these constructs (Figure 3C), this appears not to affect RNA or protein expression levels (Figures 4 and 5). This approach permits us for the first time to analyse specific alterations in the DHPS molecule against a constant genetic background outside of the dhps locus, and also represents the first systematic study of a phenotype in Plasmodium using transfection. Our analysis of the different transfectants that express mutant dhps enzymes proves that these alterations are responsible for the sulfadoxine‐resistance phenotype and allows a direct determination of the contribution of the different amino acid substitutions to the final level of drug resistance.
The most common mutation found in the sulfadoxine‐resistant isolates so far examined is A437G (Brooks et al., 1994; Triglia and Cowman, 1994; Plowe et al., 1997; Triglia et al., 1997; Wang et al., 1997a) and it has been observed in some samples to be the sole alteration in DHPS. This suggests that it is normally the first mutation selected by sulfadoxine drug pressure, although some exceptions have been seen (Wang et al., 1997a). This is supported by the observations that this single alteration leads to a 10‐fold increase in the Ki of the enzyme for sulfadoxine and can confer on transfected parasites a 5‐fold higher level of drug resistance. Thus A437G may be the closest equivalent in DHPS to the S108N alteration in P.falciparum DHFR that is almost always present in pyrimethamine‐resistant parasites (Cowman et al., 1988; Peterson et al., 1988; Wang et al., 1997a).
An additional DHPS mutation of S436A or A581G caused only a small increase in the level of sulfadoxine resistance, suggesting that these mutations play a lesser role in the phenotype. This is also in agreement with measurements for sulfadoxine using purified PPPK‐DHPS derived from the 3D7, K1 and Tak9/96 alleles, where the Ki increased by a relatively small amount in the case of each mutation (Triglia et al., 1997). This is in contrast to the addition of the K540E mutation to those at positions 436 and 437, which increased the level of sulfadoxine resistance a further 2‐fold, and ∼10‐fold with respect to the D10‐sakaa (wild‐type) transfected allele. The allele that gave the highest level of sulfadoxine resistance after transfection was W2mef, which occurs in the most highly resistant parasites found so far. The addition of S436F plus A613S to the A437G mutation increased sulfadoxine resistance in this transfectant a further 5‐fold (24‐fold relative to wild‐type) to an IC50 level of ∼3 μg/ml, very similar to that seen previously in isolates that naturally contain this allele (Wang et al., 1997b). The sulfadoxine resistance phenotypes measured in the in vitro cultures of the transfectants thus parallel closely the results seen both for the kinetic parameters of the corresponding purified enzymes (Triglia et al., 1997) and for natural isolates in culture (Wang et al., 1997b). We conclude that in these conditions the mutations observed in P.falciparum dhps are solely responsible for the sulfadoxine‐resistance phenotype.
Interestingly, transfection of the dhps allele found in sulfadoxine‐sensitive parasites consistently gave a somewhat higher level of resistance (by ∼100 ng/ml) when compared in parallel assays with the D10 parent. Analysis by immunoblots using anti‐DHPS antibodies indicated that there were approximately equal levels of expression of the PPPK‐DHPS enzyme; however, it is possible that a small elevation in protein expression, not easily apparent from the immunoblots, contributed to the increased level of resistance. Any increased expression could not be due to a more active promoter as the endogenous pppk‐dhps promoter controls expression of the inserted gene. However, the 3′ untranslated end of the expressed pppk‐dhps gene consists of hsp86 sequence, which may alter the stability of the mRNA and increase protein expression. A second possibility is that co‐transfection of a heterologous, mutant dhfr gene might have a small but significant effect on the absolute level of sulfadoxine resistance. The unnatural situation where two dhfr genes are expressed (the T.gondii gene on chromosome 8 and the P.falciparum wild‐type on chromosome 4) may affect folate pools such that sulfadoxine becomes somewhat less inhibitory. A similar effect is seen in T.gondii where transfection of the wild‐type DHFR gene increases the level of resistance to pyrimethamine (Reynolds and Roos, 1998). Whatever the cause of the baseline shift in sulfadoxine resistance, subtraction of its value from those measured for the transfectants carrying mutant alleles brings the latter into even closer agreement with previously derived figures for naturally occurring mutant strains (Wang et al., 1997b). It also suggests that the fold‐increases of resistance above the wild‐type level we report here are minimum estimates.
Although the highest values of both Ki and IC50 for the different alleles occur when the three mutations S436F, A437G and A613S are present, this combination has only rarely been seen to date in the many field samples analysed from diverse regions of the world. Most frequently, we have observed the double mutant A437G,A581G form in samples collected from areas of high‐level Fansidar resistance or from patients for whom antifolate treatment had failed (Wang et al., 1997a), indicating that this level of mutation may be sufficient in many cases to influence the outcome of Fansidar challenge. Two other triple mutant forms, the S436A,A437G,K540E type tested here and the A437G,K540E,A581G type, appear to be common in Thailand (Triglia et al., 1997) and parts of South America (Plowe et al., 1997), respectively. It is possible that the S436F,A437G,A613S form is not widely disseminated in the field because it suffers in some way from reduced fitness, despite its very high resistance to sulfadoxine.
The three‐dimensional crystal structure of the P.falciparum DHPS enzyme has not yet been obtained. However, insights can be gained into the effect of the various mutations on inhibitor and substrate binding by comparison with the recently solved structures of the Staphylococcus aureus (Hampele et al., 1997) and E.coli (Achari et al., 1997) homologues, particularly the latter, where crystals of the enzyme–inhibitor complex were analysed. Thus, an oxygen from the sulfonamide was shown to accept a hydrogen bond from the guanidinium group of Arg63 in E.coli DHPS and additionally, the aniline amine group of the drug and the carbonyl group of Thr62 are only 3.5 Å from the hydroxymethyl group of the dihydropterin substrate (Table II). Arg63 in E.coli DHPS corresponds to Gly437 in P.falciparum, which is the important mutant residue found in most sulfadoxine‐resistant parasites (Table II). Thr62 corresponds to Ser436 which, when changed to Ala or Phe, also increases sulfadoxine resistance levels in P.falciparum. This suggests that both residues 436 and 437 in P.falciparum DHPS are very close to, or involved in, both substrate‐ and inhibitor‐binding. Although the P.falciparum residues 581 and 613 do not correspond to residues in E.coli DHPS directly involved in substrate or inhibitor binding, they are next to, or very close to, residues whose bacterial counterparts are important in this binding (Achari et al., 1997). Interestingly, residue 540, where the Lys to Glu transition has a large effect on both Ki for sulfadoxine (Triglia et al., 1997) and the level of resistance in live parasites (Table I), is not close to any amino acid residues implicated in substrate or inhibitor binding. In fact, most mutations in the DHPS enzymes of S.aureus (Hampele et al., 1997) and E.coli, (Achari et al., 1997) that confer resistance to sulfa drugs are also not located in the substrate‐ or inhibitor‐binding sites, which defies a simple interpretation of their roles in the resistance mechanism.
The ability to transfect P.falciparum was developed only recently and integration occurs by a single recombination event (Crabb and Cowman, 1996; Wu et al., 1996; Crabb et al., 1997a). Previously, alleles of the dhfr‐ts gene had been transfected into P.falciparum to show that mutations in this gene (Cowman et al., 1988; Peterson et al., 1988) can confer resistance to pyrimethamine (Wu et al., 1996). However, these genes were used as the selectable marker and homologous recombination events were selected that created pseudo‐diploid and pseudo‐polyploid parasites with two or more functional copies of dhfr‐ts, one or more of which were under the control of the histidine‐rich protein 3 gene (hrp3) promoter (Wu et al., 1996). This makes it difficult to determine the contribution of specific amino acids to the particular phenotype, owing to the expression of an endogenous wild‐type gene as well as the multiple mutant dhfr‐ts genes that have integrated by homologous recombination. To overcome this problem, we transfected plasmid constructs that contained only a partial dhps gene so that integration by a single recombination event would insert the required mutations and reconstitute the gene by allelic exchange. Although insertion of multiple copies of the transfection plasmid increased the copy number of the dhps gene, the extra copies all have the 5′ end of the gene truncated and were shown not to be expressed. We were thus able to test directly the role of specific mutations in the DHPS enzyme on the sulfadoxine‐resistance phenotype.
The dhps transfectants obtained were found to have between two and four copies of the plasmid inserted into the endogenous pppk‐dhps gene. Insertion of these multiple copies was not due to challenge with higher concentrations of pyrimethamine, which would select for increased expression of DHFR, as lower levels of the drug also gave parasites with multiple inserts. It has been shown that plasmids transfected into P.falciparum replicate as episomes (Wu et al., 1995) and are present in forms larger than a single copy, probably as concatamers (Crabb et al., 1997a). Homologous recombination of such concatamers would result in insertion of multiple plasmids in a single crossover irrespective of the level of pyrimethamine used for selection. This is consistent with the observation that between one and four copies of the plasmid were inserted in independent clones obtained from the same transfection experiment.
In summary, we have used transfection to prove conclusively that the amino acid mutations identified in the DHPS enzyme of P.falciparum are responsible for the sulfadoxine‐resistance phenotype in live parasites. The demonstration that we can replace the wild‐type dhps allele to examine the effects on a drug resistance phenotype provides a powerful precedent for the analysis of other phenotypes where amino acid substitutions are implicated. This is of particular importance in P.falciparum where integration occurs via a single recombination event, precluding gene replacement by double crossover. Our studies have provided new insights into the evolution of sulfa drug resistance in P.falciparum and indicate that high level resistance results from a stepwise accumulation of mutations in the dhps gene due to selection with increasing drug pressure. This has important implications for the analysis of the development and spread of drug resistance alleles in malaria endemic areas.
Materials and methods
Parasites and transfection
Parasites were maintained (Trager and Jensen, 1978) and synchronized by standard procedures. D10 parasites were transfected as described for transient expression (Wu et al., 1995) with 150 μg plasmid DNA purified using CsCl density gradients. Parasites were cultured for 48 h post‐transfection prior to selection with 0.1 μM pyrimethamine (Crabb and Cowman, 1996). Cultures were maintained for a further 18–21 days with selection before parasites were seen. Parasites containing integrated forms of the construct were obtained by removing the drug for 3 weeks followed by further selection on 0.1 μM pyrimethamine until parasites re‐appeared. Parasites were cloned by the single‐cell method without drug selection.
Nucleic acids, pulsed‐field gel electrophoresis and DNA sequencing
Genomic DNA and intact chromosomes were extracted from trophozoites as previously described (Triglia and Cowman, 1994). Southern blotting was carried out using standard procedures. P.falciparum chromosomes were separated by PFGE using a contour‐clamped homogeneous electric field apparatus (Chu et al., 1986) at 4.2 V/cm and a pulse time of 225 s for 72 h.
Poly(A)+ RNA was purified from each cloned transfectant using the MicroFast RNA extraction kit (Invitrogen). Any remaining genomic DNA was removed by treatment with RNase‐free DNase I (Gibco‐BRL) before conversion to cDNA using the MicroFast cDNA synthesis kit (Invitrogen). For sequencing of cDNA from clones containing integrated dhps alleles, cDNA was amplified using an oligonucleotide to dhps, 5′‐GTATTTTTGTTGAACCTAAACG‐3′ (#1) and an oligonucleotide to the hsp86 3′ sequence, 5′‐TATATATGTATATTGGGGTGATG‐3′ (#2). This PCR product was sequenced with the #1 oligonucleotide and with the oligonucleotide 5′‐TCCAATTGTGTGATTTGTCCAC‐3′ (#3) (Figure 1).
Two DNA probes were used for Southern blots. The first was a 391 bp P.falciparum dhps fragment amplified using the oligonucleotides 5′‐AAGAAGAAGAACAAATTCTATAGTG‐3′ (#4) and #3. The second probe was a 210 bp T.gondii dhfr‐ts fragment amplified using the oligonucleotides 5′‐ATGCAGAAACCGGTGTGTCTGGTC‐3′ (#5) and 5′‐CGACTGATGGAGAGGGAAGTCC‐3′ (#6) as shown in Figure 1. Relative band intensities were quantitated by exposure of blots to a PhosphorImager using Image‐Quant software (Molecular Dynamics).
The full‐length pppk‐dhps genes from D10 and W2mef were obtained from the previously made pTrc constructs (Triglia et al., 1997) by digesting with BamHI–HindIII. These fragments were subcloned into pGEM11Z (Promega) which resulted in pppk‐dhps being flanked by XhoI sites at both ends. The XhoI fragments were finally cloned into the XhoI site of pHC1 resulting in D10‐FL and W2mef‐FL which were used for stable transfection (Crabb et al., 1997b).
Truncated pppk‐dhps constructs were made by a two‐step procedure. First, the AvrII site in pLITMUS 28 (New England Biolabs) was mutated to a XhoI site using PCR mutagenesis resulting in a vector with two XhoI sites flanking both a XbaI and an EcoRI site. Secondly, pTrc constructs for D10, 3D7, K1, Tak9/96, PR145 and W2mef (Triglia et al., 1997) were digested with XbaI–EcoRI, which resulted in an almost complete deletion (98 bp) of exon 1 within the pppk domain. The six truncated pppk‐dhps fragments were cloned into the XhoI‐modified pLITMUS vector and XhoI used to obtain each truncated fragment. These fragments were finally cloned into pHC1 as above (Crabb et al., 1997b) to give six constructs which, when transfected into the D10 parasite, integrated and cloned, resulted in the following parasites lines: D10‐sakaa, D10‐sgkaa, D10‐sgkga, D10‐agkaa, D10‐ageaa and D10‐fgkas (Table I).
Protein samples were analysed by SDS–PAGE and electrophoretically transferred to nitrocellulose as previously described (Triglia and Cowman, 1994). The anti‐P.falciparum DHPS antibodies were raised in rabbits to E.coli‐expressed DHPS enzyme. The antiserum was subsequently affinity‐purified (Triglia and Cowman, 1994). The antibodies to P.falciparum HSP70 protein have been previously described (Bianco et al., 1986). Bound antibody was detected with horse‐radish peroxidase coupled sheep anti‐rabbit IgG (Silenus Laboratories, Australia) and developed by ECL, a luminescence‐based method (Amersham International).
In vitro sulfadoxine assay
For determination of the sulfadoxine resistance of a cloned parasite, cultures were first transferred to a folate pABA‐free RPMI–HEPES medium containing 0.5% Albumax (Gibco‐BRL), 0.2% glucose, 1 μg/ml hypoxanthine, 50 μg/ml gentamycin and re‐supplemented with 100 ng/ml folate. After adaptation to this medium, parasites were transferred to the same medium devoid of folate for 48 h prior to drug challenge as described (Wang et al., 1997b,c). Serial dilutions of sulfadoxine were then added and cultures incubated for a further 48 h before addition of [3H]‐hypoxanthine to monitor growth. IC50 values were defined as the concentration of sulfadoxine where parasite growth was half that of untreated controls. Each IC50 value in Table I was derived from between six and eight separate experiments.
We would like to thank Drs B.Crabb and D.Roos for helpful advice and critical reading of the manuscript, and Martin Read for assistance with parasite culture. This research was supported by the Australian National Health and Medical Research Council, the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR), and a Wellcome Trust grant (No. 046643) to the UMIST group. A.F.C. was supported by an International Research Scholar's award from the Howard Hughes Medical Institute.
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