The molecular structure of oxygen‐sensitive delayed‐rectifier K+ channels which are involved in hypoxic pulmonary artery (PA) vasoconstriction has yet to be elucidated. To address this problem, we identified the Shab K+ channel Kv2.1 and a novel Shab‐like subunit Kv9.3, in rat PA myocytes. Kv9.3 encodes an electrically silent subunit which associates with Kv2.1 and modulates its biophysical properties. The Kv2.1/9.3 heteromultimer, unlike Kv2.1, opens in the voltage range of the resting membrane potential of PA myocytes. Moreover, we demonstrate that the activity of Kv2.1/Kv9.3 is tightly controlled by internal ATP and is reversibly inhibited by hypoxia. In conclusion, we propose that metabolic regulation of the Kv2.1/Kv9.3 heteromultimer may play an important role in hypoxic PA vasoconstriction and in the possible development of PA hypertension.
Oxygen is an essential requirement for cell survival. However, the organism has the ability to adapt rapidly to hypoxia. Ion channels, in particular K+ channels, play a key role in adaptive hypoxic mechanisms and are thought to be involved in the sensing of oxygen (Lopez‐Barneo, 1994, 1996; Kozlowski, 1995; Weir and Archer, 1995; Lopez‐Barneo et al., 1997). One such adaptation is the hypoxia‐induced vasoconstriction of resistance pulmonary artery (PA) smooth muscle which leads to a redistribution of the non‐oxygenated blood towards better ventilated regions of the lung (for review, see Kozlowski, 1995; Weir and Archer, 1995). In the fetus, hypoxic pulmonary vasoconstriction (HPV) diverts blood through the ductus arteriosus and is essential for fetal survival. Although HPV fulfils an essential physiological function, it also contributes to the development of pulmonary hypertension in patients with chronic obstructive lung diseases (e.g. chronic bronchitis, emphysema) and people living at high altitudes (Barnes and Liu, 1995; Weir and Archer, 1995). It has been demonstrated that hypoxic vasoconstriction of resistance PA smooth muscle cells is mainly mediated by the closing of voltage‐dependent K+ channels leading to cell depolarization, calcium influx and myocyte contraction (Post et al., 1992; Archer et al., 1993; Yuan et al., 1993a; Cornfield et al., 1994; Osipenko et al., 1997). Chronic hypoxia has also been associated with reduced delayed‐rectifier K+ current in rat pulmonary artery smooth muscle cells (Smirnov et al., 1994). Recently, a novel oxygen‐sensitive non‐inactivating K+ current active at resting membrane potential (IK(N)) has been described in rabbit PA myocytes (Evans et al., 1996; Osipenko et al., 1997).
The molecular identity of these oxygen‐sensitive K+ channels and the nature of the oxygen sensor(s) remain unknown. The present work reports the molecular cloning, characterization and regulation by hypoxia of a novel voltage‐ and ATP‐dependent K+ channel subunit called Kv9.3, isolated from rat PA smooth muscle cells. The molecular identification of Kv9.3 could be of tremendous importance in the understanding of pulmonary hypertension and in the design of novel therapeutic strategies.
Delayed‐rectifier K+ channels in rat pulmonary artery myocytes
In order to record selectively the activity of voltage‐dependent K+ channels in PA smooth muscle, cells were voltage‐clamped in the whole‐cell configuration in the absence of external and internal calcium (10 mM EGTA) and in the presence of 5 mM internal ATP to avoid contamination with both large conductance calcium‐dependent K+ channels and KATP channels (Yuan et al., 1996). Figure 1A and B compare the I–V relationships of the currents recorded in freshly dissociated and cultured PA cells. The characteristics of the outward currents observed in freshly dissociated cells were identical to those in primary (I) culture. However, when the cells were trypsinized and plated in secondary (II) culture, the outward K+ currents were absent (Figure 1B). Similar data were obtained with passages III to VIII (not shown). During maintained depolarization, the outward K+ current declined with an exponential time course (τ = 271 ± 48 ms at + 50 mV, n = 18). Steady‐state activation and inactivation parameters of currents recorded in primary cultured PA cells are presented in Figure 1C. The activation voltage threshold was −50 mV, a value close to the resting membrane potential (−54 ± 4 mV, n = 6). About 15% of the channels failed to inactivate at +20 mV (Figure 1C). The activation and inactivation steady‐state curves overlapped, revealing a window K+ current. The pharmacological properties of the PA myocyte K+ channels are illustrated in Figure 1D. Charybdotoxin (CTX; 20 nM), dendrotoxin (DTX; 200 nM) and mast cell degranulating peptide (MCD; 300 nM), potent blockers of both Kv1.2 and Kv1.3, inhibited the outward current by ∼20%, with the effects of DTX and CTX being non‐additive. In agreement with previous reports (Post et al., 1995; Yuan, 1995; Archer et al., 1996), we observed that outward K+ currents in PA smooth muscle cells are relatively resistant to tetraethylammonium (TEA) but sensitive to 4‐aminopyridine (4‐AP). Finally, the appetite‐suppressant drug dex‐fenfluramine (DFF) which has been shown to induce PA vasoconstriction (Weir et al., 1996) inhibited the outward K+ current by 60% at a concentration of 1 mM. The sensitivity of resistance PA myocytes K+ channels to hypoxia is illustrated in Figure 1E. The hypoxic inhibition of K+ currents in the presence and in the absence of 200 nM DTX was compared (Figure 1F). Hypoxic inhibitions (25 ± 4% and 17 ± 2% in control and in the presence of DTX, respectively) were not statistically different (P <0.05) between the two experimental conditions.
Cloning of delayed‐rectifier K+ channel subunits from pulmonary artery myocytes
A degenerate PCR‐based strategy was used to identify at the molecular level, potential PA oxygen‐sensitive K+ channels. Our degenerate primers were designed in two highly conserved regions in Shaker, Shab, Shaw and Shal family members, namely a region upstream of S1 (the first transmembrane domain) and a region in the pore (the H5 domain) (Figure 2A). Following this procedure, Kv1.2, Kv1.3, Kv2.1 and a novel K+ channel, Kv9.3 were identified in PA myocytes (Figures 2A and 3A). As negative controls, specific oligonucleotides for Kv1.5 (Figure 3A) and Kv2.2 (data not shown) were used to show the absence of these transcripts in PA cell templates (conduit and resistance) although a strong signal was always observed within the brain. The amino acid sequence of Kv9.3 (through S6) is aligned with that of Kv2.1 in Figure 2A. Kv9.3 bears all the classical hallmarks of a voltage‐gated K+ channel with six potential transmembrane domains (S1–S6), S4 containing five positively‐charged amino acids and a conserved pore region, H5, between S5 and S6. Alignment of Kv9.3 with other voltage‐gated K+ channels shows that it is most homologous to Shab family members, sharing the highest identity with Kv9.2 at 52.4%. The phylogenetic tree presented in Figure 2B demonstrates that Kv9.3 belongs to a novel family of electrically silent K+ channel subunits including Kv5, Kv6, Kv8 and Kv9 (Salinas et al., 1997b) members. Northern blot analysis of various rat tissues reveals a single transcript for Kv9.3 at 3.2 kb, the most abundant of which is in lung (Figure 3B). In order to define the spatial expression of Kv1.2, Kv1.3, Kv2.1 and Kv9.3, RT–PCR was performed with RNA from different tissues including lung, brain, conduit artery and resistance artery cells (primary and secondary cultures) (Figure 3A). All the transcripts were found in the lung and brain. A differential pattern of expression is observed between conduit and resistance PA cells. Kv1.2 and Kv2.1 are expressed at higher levels in conduit artery. By contrast, Kv1.3 is only expressed in resistance artery whereas, Kv9.3 was similarly expressed in both tissues. Furthermore, only Kv9.3 was detected in cells maintained in secondary culture.
Functional expression of Kv9.3 in COS cells and Xenopus oocytes
The cDNA encoding Kv9.3 was cloned into the expression vector pCI and either transfected in COS‐M6 cells or injected into Xenopus oocyte nuclei (Figure 4). pCI–Kv9.3‐expressing cells did not express any exogenous channel activity (Figure 4B). However, when pCI–Kv9.3 was co‐expressed with pCI–Kv2.1 (also identified in PA cells), we observed an important modification of the Kv2.1 channel properties recorded in a physiological K+ gradient (Figure 4A and B). The activation threshold was shifted towards negative values (−50 mV) and the amplitude of the currents were enhanced in the presence of Kv9.3 (Figure 4B). Control oocytes had a RMP of −34 ± 3 mV (n = 10) and Kv2.1 and Kv2.1/Kv9.3‐expressing oocytes displayed RMPs of −53 ± 4 mV (n = 8) and −58 ± 4 mV (n = 9), respectively. The RMPs of control, Kv9.3‐, Kv2.1‐ and Kv2.1/Kv9.3‐expressing COS cells were 3.9 ± 5 mV (n = 10), 6.3 ± 3.6 mV (n = 7), −30.7 ± 1.3 mV (n = 10) and −50.6 ± 0.9 mV (n = 15), respectively. Tail current analysis was used to estimate the reversal potentials and to construct steady‐state activation curves. The reversal potential of the tail currents was identical for Kv2.1 (−84 ± 3 mV, n = 7) and Kv2.1/Kv9.3 (−86 ± 4 mV, n = 8). Both steady‐state activation and inactivation curves of Kv2.1 were displaced towards more negative values by ∼20 mV in the presence of Kv9.3 (Figure 4C). Besides the alteration of the steady‐state activation and inactivation parameters, Kv9.3 also had an important effect on the activation and deactivation kinetics (Figure 4A and D–F). Kv9.3 speeded up the activation and slowed down the deactivation of Kv2.1. To verify that the effects of Kv9.3 were specific for Kv2.1, pCI–Kv9.3 was co‐injected with either pCI–Kv1.2 or pCI–Kv1.3 (also identified in PA cells) into Xenopus oocytes. No effect was found on the biophysical properties of both Kv1.2 and Kv1.3 (n = 6; data not shown).
Single‐channel properties of Kv2.1/Kv9.3
Examination of the single‐channel properties of Kv2.1 and Kv2.1/Kv9.3 revealed that Kv9.3 alters the single‐channel conductance of Kv2.1 (Figure 5A and B). In the cell‐attached mode and in inside‐out patch configuration, Kv2.1 has a single‐channel conductance of 8.5 and 6.2 pS, respectively (physiological K+ gradient). However, when co‐expressed with Kv9.3, the single‐channel conductance was 14.5 pS and 12.7 pS (cell‐attached and inside‐out configurations). In some patches, from cells co‐expressing Kv9.3 and Kv2.1, both single‐channel conductances (8.5 and 14.5 pS) were observed. No intermediate single‐channel conductance was observed in patches from Kv2.1/Kv9.3‐expressing cells. Figure 5C shows single‐channel recordings from a cell‐attached patch at different potentials and demonstrates that the open state probability of Kv2.1/Kv9.3 was dependent on the voltage as expected from the measured whole‐cell currents (Figure 4). The I–V curve measured using a voltage ramp protocol shows that the activation threshold was −50 mV and that a mild inward rectification was observed at positive potentials (Figure 5D).
Kv2.1/Kv9.3 is regulated by internal ATP
The activity of both Kv2.1 and Kv2.1/Kv9.3 in excised inside‐out patches was sensitive to the presence of internal ATP. Figure 6A compares the effects of various ATP concentration conditions and the effects of ATPγs and AMP–PNP on Kv2.1/Kv9.3 channel activity. When ATP concentration was lowered from 5 mM to 1 mM, channel activity was reduced by 55%. ATP‐free solution or substitution of ATP by AMP–PNP both produced a decrease of ∼80% in Kv2.1/Kv9.3 channel activity. However, when ATP was substituted by ATPγs, channel activity was only reduced by 50%. Similar data were obtained with Kv2.1 alone (not shown). Channel activity was insensitive to internal ADP (Figure 6A). The possible implication of internal ATP in channel phosphorylation was then studied using alkaline phosphatase (n = 5). Figure 6B and C show that channel activity observed in the presence of 5 mM internal ATP could be reversibly inhibited by alkaline phosphatase.
Pharmacological properties of the Kv2.1/Kv9.3 channel
Figure 7 shows that the pharmacological properties of Kv2.1 were altered when co‐expressed with Kv9.3 in Xenopus oocytes. Both the sensitivity to TEA and 4‐AP were decreased by 3‐ and 10‐ fold, respectively (Figure 7A and B). Figure 7C shows the effect of 4‐AP on the I–V curve of Kv2.1/Kv9.3 recorded in a K+‐rich solution. This protocol allows the amplification of K+‐currents at negative potentials, in the range of the activation threshold and demonstrates that 4‐AP shifts the mid‐point activation potential of Kv2.1/Kv9.3. Kv2.1 and Kv2.1/Kv9.3 were insensitive to 50 nM CTX and 200 nM DTX (n = 3, not shown). Figure 7D and E shows that DFF is a potent blocker of Kv2.1 and Kv2.1/Kv9.3. Both channels displayed the same sensitivity to DFF (IC50 300 μM). The effect of increasing concentrations of DFF on the Kv2.1/Kv9.3 I–V curve recorded in COS cells is shown in Figure 7E.
Co‐immunoprecipitation of Kv9.3 with Kv2.1
The previous data suggested that Kv9.3 may associate with Kv2.1 to form a heteromultimer whose biophysical and pharmacological properties were different from the Kv2.1 homomultimer. In order to demonstrate a direct interaction between Kv2.1 and Kv9.3, immunoprecipitation experiments were performed with metabolically labelled Xenopus oocytes injected with pCI–Kv9.3, pCI–Kv2.1, or both. Figure 8 shows that anti‐Kv2.1 immunoprecipitates three major species (see inset) in Kv2.1‐expressing oocytes in the range of 110 kDa, which probably correspond to phosphorylated forms of Kv2.1 as has been previously reported (Shi et al., 1994). No expression of immunoreactive Kv2.1 protein was detected in control oocytes (lane 1). However, when Kv2.1 was co‐expressed with Kv9.3, an additional band of ∼56 kDa was detected. This band migrates in the same size range as the in vitro‐translated Kv9.3 (data not shown) and is absent in control oocytes. Protein A–Sepharose alone did not precipitate either Kv2.1 or Kv9.3 (data not shown).
Effects of hypoxia on Kv2.1 and Kv2.1/Kv9.3
In a subset of transfected COS cells, Kv2.1 (21% of the cells, n = 14) and Kv2.1/Kv9.3 (56% of the cells, n = 39) were reversibly inhibited by hypoxia (effect >10% inhibition). Figure 9 shows the reversible hypoxic inhibition of Kv2.1 and Kv2.1/Kv9.3. The mean hypoxic inhibitions of the responsive cells were 34 ± 5.5% and 28 ± 2.5% for Kv2.1 and Kv2.1/Kv9.3, respectively. Kv1.2 and Kv1.3 did not display any hypoxic sensitivity in COS cells (n = 11; data not shown). The I–V curves illustrated in Figure 9D show that the effects of hypoxia were independent of the voltage.
Molecular identification of K+ channel subunits in rat PA smooth muscle cells
The aim of the present work was to identify the oxygen‐sensitive voltage‐dependent delayed‐rectifier K+ channels in rat pulmonary artery smooth muscle cells. Using a PCR approach, we isolated two Shaker subunits Kv1.2 and Kv1.3 (Baumann et al., 1988; Stuhmer et al., 1989; Beckh and Pongs, 1990; Pongs, 1992), one Shab subunit Kv2.1 (Frech et al., 1989) and a novel Shab‐related subunit called Kv9.3. The presence of Kv1.2 and Kv1.3 was confirmed at the pharmacological level in PA cells using CTX (blocker of both Kv1.2 and Kv1.3) and DTX and MCD peptide (blockers of Kv1.2). This analysis revealed that the DTX‐sensitive channels underly ∼20% of the total K+ channel current in PA cells and do not play a significant role in the oxygen‐sensitive K+ channel complexes. Previous reports have clearly demonstrated that hypoxic pulmonary artery vasoconstriction is unaffected by TEA and CTX, blockers of both Kv1.2 and Kv1.3 (Post et al., 1995; Yuan, 1995; Archer et al., 1996). Therefore, these and our present data strongly suggest that Kv1.2 and Kv1.3 do not play a major role as oxygen‐sensitive K+ channels in PA myocytes. Delayed‐rectifier K+ channels IK(V) and IK(A) have been identified in PA myocytes (Post et al., 1992; Yuan et al., 1993b; Smirnov et al., 1994; Yuan, 1995; Archer, 1996; Archer et al., 1996). IK(V) activates and inactivates rather slowly, whereas IK(A) is a transient current. Kv1.3 shares similarities with the native IK(A) channel recorded in PA myocytes (Honoré et al., 1992), while the existence of both Kv2.1 and Kv1.2 may account for IK(V). Since Kv2.1, Kv1.2 and Kv1.3 are all sensitive to 4‐AP, they may account for the 4‐AP‐sensitive component of PA myocytes.
Kv9.3 associates with Kv2.1 and alters its biophysical and pharmacological properties
The novel Shab‐related subunit, Kv9.3 encodes an electrically silent K+ channel subunit when expressed in Xenopus oocytes and COS cells. However, this silent channel causes important alterations in the biophysical properties of the Shab channel Kv2.1. In particular, Kv9.3 consistently increased Kv2.1 channel current amplitude and shifted steady‐state activation towards negative values (−60 to −50 mV). Furthermore, we observed that Kv2.1/Kv9.3 expression induced a shift in the resting membrane potential of both Xenopus oocytes and transfected COS cells (from +4 to −51 mV). The ability of Kv9.3 to ‘drag’ the Kv2.1 activation voltage threshold into the range of PA myocytes RMP suggests that the Kv2.1/Kv9.3 channel complex may contribute to the setting of the RMP (−54 ± 4 mV) and, consequently, in the setting of the resting pulmonary arterial pressure. Kv9.3 also speeded up Kv2.1 activation and dramatically slowed down deactivation. The slowing down of the deactivation was particularly evident in the voltage range corresponding to the resting membrane potential (RMP) of PA myocytes (−50 mV). The steep voltage‐dependency of Kv2.1/Kv9.3 suggests that besides its possible role in the establishment of the RMP, this channel may also play an essential role in the control of the PA myocyte action potential duration. Recently, a novel K+ current IK(N) that is active at resting membrane potential, has been described in rabbit PA smooth muscle cells (Evans et al., 1996; Osipenko et al., 1997). This current is voltage‐gated with an activation threshold between −80 and −65 mV in K+‐rich solution. IK(N) was recorded at all levels of the pulmonary artery tree and was found to be blocked by 4‐AP with 50% inhibition at 10 mM (Evans et al., 1996). Most importantly, the close similarity between the biophysical and pharmacological characteristics of IK(N) and the IKv2.1/Kv9.3 suggests that the channels underlying these currents may be one and the same.
The alterations in the macroscopic properties of Kv2.1 channel current were confirmed at the single‐channel level. Kv9.3 increased the single‐channel conductance of Kv2.1, from 8.5 to 14.5 pS. At the pharmacological level, the sensitivity to both 4‐AP and TEA were reduced when Kv2.1 was co‐expressed with Kv9.3. The alterations in the biophysical and pharmacological properties of Kv2.1 by Kv9.3 suggest that these two subunits associate to form a heteromultimer whose properties are different from that of the Kv2.1 homomultimer. Indeed, immunoprecipitation experiments demonstrated a direct interaction between Kv2.1 and Kv9.3.
Kv9.3 belongs to a novel family of electrically silent K+ channels and is the first to be identified in vascular smooth muscle. Other members of this new family have previously been identified in brain (Drewe et al., 1992; Hugnot et al., 1996; Salinas et al., 1997a). These channel subunits do not express a K+ channel current by themselves, but induce profound changes in the properties of the Shab channels Kv2.1 and Kv2.2 (Hugnot et al., 1996; Post et al., 1996; Salinas et al., 1997a). Most interestingly, these silent subunits have the ability to create a diverse range of effects, since Kv8.1 acts as a dominant inhibitory subunit (Hugnot et al., 1996) while Kv9.3 behaves as a stimulatory one (the present report).
The Kv2.1/Kv9.3 complex is modulated by internal ATP
Our electrophysiological data demonstrate that internal ATP plays an essential role in the control of Kv2.1 and Kv2.1/Kv9.3 channel activity. In inside‐out patches, removal of ATP reversibly inhibits channel activity. This is in contrast to what is observed for KATP channels which are blocked by the presence of ATP (for review, see Quast and Cook, 1989). Similar inhibition was obtained when ATP was substituted with the poorly hydrolysable ATP analogue AMP–PNP. Moreover, the inhibition induced by alkaline phosphatase and the partial activation by ATPγs favour the hypothesis of an ATP regulation via a phosphorylation mechanism. The effects of ATP‐free solution and alkaline phosphatase could be obtained repetitively with complete recovery after washout in the absence of any added kinase. This indicates that an endogenous kinase may be closely associated with the channel or may even be a structural part of the channel structure itself. Both Kv2.1 and Kv9.3 contain numerous putative phosphorylation sites including PKA, PKC and calcium–calmodulin kinase sites. Additionally, Kv9.3 contains a putative phosphorylation site for tyrosine kinase. Further studies will be required to identify the exact nature of the modulation and the phosphorylation sites involved in the regulation of the Kv2.1/Kv9.3 complex by internal ATP.
Hypoxic inhibition of Kv2.1 and Kv2.1/Kv9.3
It has been hypothesized that the oxygen sensor could be a structural part of the oxygen‐sensitive K+ channels (for review, see Weir and Archer, 1995). During normoxia, the channel would be in an oxidized open state and during hypoxia, the reduced form of the channel would be closed (Ruppersberg et al., 1991; Duprat et al., 1995). Another hypothesis suggested that the K+ channels expressed in the oxygen‐sensitive cells (PA, type I carotid body and pulmonary neuroepithelial body cells) are closely associated with a membrane‐bound oxygen sensor such as an NADPH oxidase (Youngson et al., 1993; Wang et al., 1996). Alternatively, oxygen regulation has been proposed to be a consequence of variation in the level of intracellular metabolites such as ATP, oxyradicals, cytochrome P450 products and intracellular calcium (Archer et al., 1993; Yuan et al., 1994, 1995a,b, 1996; Bright et al., 1995; Post et al., 1995).
In the present study, we observed that in a subset of transfected COS cells, Kv2.1 and Kv2.1/Kv9.3 were reversibly inhibited by hypoxia. Since hypoxic sensitivity was not detected in all the cells tested, these results imply that the oxygen sensor is not a structural part of the Kv2.1/Kv9.3 complex, but rather an oxygen reactive structure endogenously expressed in only a subset of our COS cells.
Very recently, it has been demonstrated that the IK(N) current which is active at resting potential is reversibly inhibited by hypoxia and is involved in the hypoxic depolarization of rabbit PA myocytes (Osipenko et al., 1997). Considering the biophysical, pharmacological and hypoxic regulation properties of IK(N), it is tempting to suggest that Kv2.1/Kv9.3 may encode IK(N) in PA smooth muscle cells.
Metabolic regulation of Kv2.1/Kv9.3 and hypoxic pulmonary artery vasoconstriction
Our finding that intracellular ATP activates, and that hypoxia inhibits Kv2.1 and Kv2.1/Kv9.3, raises the question of the role of this regulation in PA smooth muscle cells and their possible implications for hypoxic pulmonary vasoconstriction (HPV). Several arguments strongly suggest that the PA hypoxic contractile response is at least partly dependent on the glycolytic pathway. First, in the perfused rat lung, inhibitors of oxidative phosphorylation and glycolysis cause transient pressor responses and potentiate hypoxic vasoconstriction (Rounds and McMurtry, 1981). Secondly, 2‐deoxyglucose and carbonyl cyanide p‐trifluoromethoxyphenylhydrazone (FCCP) decrease the activity of voltage‐dependent K+ channels, raise intracellular Ca2+ and trigger contraction of isolated PA smooth muscle cells (Yuan et al., 1994, 1996). Thirdly, rotenone and antimycin A which have been demonstrated to decrease the production of activated oxygen species from the electron transport chain and the synthesis of ATP, inhibit K+ currents in PA myocytes and mimic HPV (Archer et al., 1993). Fourthly, a direct effect of intracellular ATP on Kv channels in rabbit PA smooth muscle cells has been demonstrated, whereby an elevation in intracellular ATP levels induced an important increase in the delayed‐rectifier K+ current amplitude (Evans et al., 1994). Taken together, these data suggest that the delayed‐rectifier K+ channels, among them Kv2.1 and Kv2.1/Kv9.3, are regulated by local metabolic conditions in PA cells and that their activities are tightly linked to internal ATP and possibly to activated oxygen species from the electron transport chain. Most importantly, given the ATP sensitivity in the millimolar range of both Kv2.1 and Kv2.1/Kv9.3 near physiological concentrations, these channel complexes must be extremely sensitive to the metabolic status of the cell.
Segmental expression of Kv2.1/Kv9.3 in pulmonary artery
It has been suggested that a differential distribution of voltage‐dependent, delayed‐rectifier K+ channels in conduit and resistance arteries determines their response to hypoxia (Archer et al., 1996). Hypoxia causes sustained constriction of resistance arteries and conversely relaxation in conduit arteries (Bennie et al., 1991; Jin et al., 1992) These effects have been correlated with a higher expression of oxygen‐reactive, delayed‐rectifier K+ channels in resistance arteries (Archer, 1996; Archer et al., 1996). By contrast the oxygen‐sensitive IK(N) was found at all levels along the PA tree (Evans et al., 1996; Osipenko et al., 1997).
Kv2.1 transcript levels were higher in conduit compared with resistance arteries, while Kv9.3 was expressed at similar levels in both segments. These results suggest that differential translational and/or post‐translational controls rather than transcriptional control may be involved in the segmental expression of oxygen‐sensitive delayed‐rectifier IK(V) and IK(N) channels.
The anorexic agent dex‐fenfluramine blocks Kv2.1 and Kv2.1/Kv9.3 channels
Pulmonary hypertension is a dramatic disease which ultimately provokes right heart failure and death within 2 to 5 years. It occurs in people living at high altitude and in patients suffering from chronic obstructive lung diseases such as chronic bronchitis and emphysema (Barnes and Liu, 1995). Furthermore, it has been demonstrated that anorexic agents such as aminorex fumarate and DFF have caused an epidemic of pulmonary hypertension in Europe. Interestingly, these compounds have been recently shown to inhibit K+ currents in rat pulmonary artery myocytes and to cause pulmonary vasoconstriction (Weir et al., 1996). In the present report we confirm these data and further demonstrate that Kv2.1 and Kv2.1/Kv9.3 complexes are similarly sensitive to the hypertensive anorexic agent, DFF.
In conclusion, our results support the idea that, in PA myocytes, the Kv2.1 and Kv2.1/Kv9.3 complexes are components of the ATP‐sensitive delayed‐rectifier K+ channels. The voltage activation threshold of the Kv2.1/Kv9.3 complex indicates that it may play a role in the establishment of the resting membrane potential of PA myocytes and in the control of the action potential duration. The biophysical, pharmacological and regulation profiles of Kv2.1/Kv9.3 are identical to those of the IK(N) channels in PA myocytes, including sensitivity to 4‐AP, resistance to TEA and CTX, and inhibition by hypoxia. The understanding of the molecular nature of the K+ channels in PA myocytes will obviously have major therapeutic significance for important human pathologies such as pulmonary hypertension. An interesting issue would be to identify openers of these novel ATP‐dependent, delayed‐rectifier K+ channels. Opening of these channels would lead to repolarization of PA myocytes, closing of calcium channels, vasorelaxation of resistance arteries and thereby a decrease in arterial pressure. Moreover, the identification of these channels may help to define novel appetite‐suppressant agents devoid of side effects on the pulmonary vasculature.
Materials and methods
Isolation of K+ channel cDNAs
Degenerate primers were designed in two highly conserved regions of K+ channels, one upstream of S1 (5′‐ISARKWITWYTWYGAYMG‐3′) and the other in the H5 pore domain (5′‐RTAICCIACIGTISWCAT‐3′). These primers were used to amplify reverse‐transcribed total RNA from dissected pulmonary arteries (PA) freed of the endothelium (as described in ‘Electrophysiological studies’) with 40 rounds of PCR (total reaction volume 50 μl: 20 mM Tris–Cl, pH 8.4, 50 mM KCl, 2 mM MgCl2, 400 μM dNTPs, 10 μM each primer, 2.5 U of Gibco‐BRL Taq polymerase and 100 ng of PA cDNA). Cycling parameters were: denaturation for 5 min at 95°C, followed by 10 cycles of 95°C for 45 s, 45°C for 2 min, 72°C for 1 min and an additional 30 cycles of 95°C for 45 s, 50°C for 2 min, 72°C for 1 min. Ten μl of the PCR reaction was run on LMP (expected size between 900 and 1000 bp) and the amplified band excised and subjected to another round of PCR. Two μl of the resulting PCR reaction was cloned into the TA cloning vector (Invitrogen). The partial cDNAs obtained in this manner were subsequently sequenced (automatic sequencer, Applied Biosystems) and used in a BLAST search, leading to the identification of Kv1.2, Kv1.3, Kv2.1 and a novel gene Kv9.3 which was used to screen 106 clones of a λZAP rat brain cDNA library (Stratagene) at high stringency (50% formamide, 5× SSC, 4× Denhardt, 1% SDS, 100 μg/ml herring sperm DNA, 55°C). Two independent clones were obtained which did not correspond to the full‐length cDNA and lacked the 5′ end. The 5′ end was obtained from a rat kidney Lambda Fix II genomic library (Stratagene) and confirmed by RT–PCR.
Northern blot analysis
Total RNA was extracted from rat tissues following Chomcyzynski and Sacchi's procedure. Northern blot analysis was performed using 15 μg of total RNA. The probe used corresponded to the initial degenerate PCR fragment of 930 bp and was labelled using the random prime kit, Prime‐It (Stratagene) and [α‐32P]dCTP. Hybridization was carried out at high stringency (same conditions as for the library).
One μg of total RNA was reverse‐transcribed using 250 ng of random primers and SUPERSCRIPT II reverse transcriptase according to the manufacturer's protocol (Gibco‐BRL). PCR was performed in a volume of 25 μl in the presence of 1.5 μCi of [α‐32P]dCTP, 1.5 mM MgCl2, 20 mM Tris–Cl, pH 8.4, 50 mM KCl, 100 ng of each primer, 200 μM dNTPs and 50 ng of template. Cycling parameters: 95°C for 5 min, followed by 35 cycles of 94°C for 45 s, X°C for 45 s (where X is the annealing temperature), 72°C for 1 min. Two μl of each reaction was resolved on a 5% polyacrylamide gel. The gel was dried and exposed to film. PCR primer sets, predicted sizes and annealing temperatures are as listed in Table I.
Metabolic labelling with [35S]methionine and immunoprecipitation
Xenopus oocyte nuclei were injected with either 100 pg pCI (Promega), 100 pg pCI–Kv9.3, 100 pg pCI–Kv2.1 or 100 pg of pCI–Kv9.3 and 100 pg of pCI–Kv2.1 (final DNA concentrations were adjusted with pCI empty expression vector), incubated for 4 h in ND96, and then in ND96 containing 50 μCi/ml [35S]methionine for 2–3 days at 22°C. Eight oocytes were homogenized in 300 μl of solubilization buffer (20 mM Tris–Cl, pH 7.4, 5 mM EDTA, 5 mM EGTA, 100 mM NaCl, 50 μg/ml PMSF, 1 mM iodoacetamide, 1 μM pepstatin) and centrifuged at 1000 g for 10 min at 4°C. Triton X‐100 was added to the supernatant to a final concentration of 4% and the homogenates were spun as before. The supernatant was diluted to 1 ml with solubilization buffer and then incubated with a 1:200 dilution of anti‐Kv2.1 (Upstate biotechnology) for 16 h at 4°C. The antibody–antigen complex was incubated for 1 h with protein A–Sepharose (Pharmacia) and then pelleted by brief centrifugation. Immunoprecipitates were washed four times with solubilization buffer, once with PBS and then resuspended in 50 μl of SDS sample buffer. The samples were resolved (without boiling) on a 7.5% polyacrylamide–SDS gel, with molecular weight standards, the gel dried down and exposed to film.
Oocyte injections and COS transfections
100 pg of pCI–Kv2.1 and 200 pg pCI–Kv9.3 were routinely injected (10 nl) into Xenopus oocyte nuclei. COS‐M6 cells were transfected with 1 μg of pCI–Kv2.1, 2 μg of pCI–Kv9.3 and 1 μg of pCI–CD8 (as a marker of transfection) using the standard DEAE–dextran procedure. Final DNA concentrations were always adjusted with pCI empty expression vector.
Conduit (main right and left PA with an external diameter of 500–600 μm) and resistance (fourth and fifth divisions with a diameter <300 μm) pulmonary arteries were dissected in aseptic conditions from 1‐month‐old Sprague‐Dawley rats. The pulmonary branches were cleaned, under a dissecting microscope, of all visible parenchyma and connective tissues. The endothelium was removed mechanically by threading the vessels onto a lightly sanded surgical steel rod and rotating the vessel four times in each direction. Cell isolation procedures are identical to those of Guibert et al. (1996). PA myocytes were cultured in H21 medium supplemented with 15% fetal bovine serum. Cells were passaged every week. Electrophysiological procedures (oocyte two microelectrode voltage clamp, COS whole cell and single channel patch clamp) have been previously described elsewhere (Honoré et al., 1992).
For COS whole‐cell experiments, we used an external medium of the following composition: Na+ gluconate, 170 mM; KCl, 5 mM; CaCl2, 1 mM; MgCl2, 3 mM; HEPES, 10 mM; pH 7.4 with NaOH. The internal media was: K+ gluconate, 150 mM; MgCl2, 3 mM; EGTA, 10 mM; HEPES, 10 mM; Na2ATP, 5 mM; pH 7.2 with KOH. For cell‐attached single‐channel studies, cells were bathed with a K+‐rich solution of the following composition: K+ gluconate, 150 mM; MgCl2, 3 mM; EGTA, 10 mM; HEPES, 10 mM; pH 7.2 with KOH and the pipette contained the external solution used for whole‐cell experiments. For pulmonary artery myocyte electrophysiology, we used an external medium of the following composition: NaCl, 141 mM; KCl, 4.7 mM; MgCl2, 3 mM; HEPES, 10 mM; EGTA, 5 mM; glucose, 10 mM; pH 7.4 with NaOH. The internal solution contained: KCl, 125 mM; MgCl2, 4 mM; HEPES, 10 mM; EGTA, 10 mM; Na2ATP, 5 mM, pH 7.2 with KOH. For oocyte experiments we used ND96 saline solution. The effects of hypoxia were studied by switching between normoxic and hypoxic perfusate reservoirs. The hypoxic perfusate reservoir was bubbled at least 20 min before the beginning of perfusion with 100% N2. These procedures produced pO2s of 160 mmHg in normoxia and 35 mmHg in hypoxia in the recording chamber. The pH and temperature were maintained at 7.4 and 25°C respectively, throughout the experiments. Rate of solution exchange was <10 s. Alkaline phosphatase, ATPγs, AMP–PNP and ADP were obtained from Sigma. CTX, DTX and MCD peptide were purified in our laboratory. Dex‐fenfluramine was a gift from Servier (Paris). Dex‐fenfluramine was dissolved daily as a stock solution in saline solution at a concentration of 10 mM.
We are grateful to Dr F.Lesage for his advice during the progress of this work and to all the members of our laboratory for fruitful discussion. We thank D.Doume for her secretarial assistance. This work was funded by the Centre National de la Recherche Scientifique (CNRS), the Philippe Foundation and by the Marie Curie EEC programme.
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